{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:57:03Z","timestamp":1791910623579,"version":"4.3.4"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary and Animal Science"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.vas.2026.100802","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T06:55:03Z","timestamp":1786172103000},"page":"100802","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Supplementation of lupeol during in vitro maturation improves preimplantation embryonic development in pigs"],"prefix":"10.1016","volume":"33","author":[{"given":"Qingyi","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshige","family":"Otoi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oky Setyo","family":"Widodo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theerawat","family":"Tharasanit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaywalee","family":"Chatdarong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rentsenkhand","family":"Sambuu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megumi","family":"Nagahara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Hirata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuminori","family":"Tanihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Namula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vas.2026.100802_bib0001","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s13596-019-00409-6","article-title":"Antimicrobial, cytotoxic, phytochemical and biological properties of crude extract and solid phase fractions of Monotheca buxifolia","volume":"20","author":"Ali","year":"2020","journal-title":"Advances in Traditional Medicine"},{"issue":"4","key":"10.1016\/j.vas.2026.100802_bib0002","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.1016\/0006-291X(77)91428-0","article-title":"The effect of dimethylsulfoxide and other hydroxyl radical scavengers on the oxidation of ethanol by rat liver microsomes","volume":"78","author":"Cederbaum","year":"1977","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"3","key":"10.1016\/j.vas.2026.100802_bib0003","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1111\/j.1740-0929.2011.00883.x","article-title":"In vitro production of porcine embryos: Current status, future perspectives and alternative applications","volume":"82","author":"Dang-Nguyen","year":"2011","journal-title":"Animal Science Journal"},{"issue":"1","key":"10.1016\/j.vas.2026.100802_bib0004","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1530\/ror.0.0020048","article-title":"Reactive oxygen species and sperm physiology","volume":"2","author":"De Lamirande","year":"1997","journal-title":"Reviews of Reproduction"},{"issue":"2","key":"10.1016\/j.vas.2026.100802_bib0005","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.repbio.2015.01.002","article-title":"Astaxanthin present in the maturation medium reduces negative effects of heat shock on the developmental competence of porcine oocytes","volume":"15","author":"Do","year":"2015","journal-title":"Reproductive Biology"},{"issue":"1","key":"10.1016\/j.vas.2026.100802_bib0006","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1002\/mrd.23440","article-title":"Procyanidin B1 promotes in vitro maturation of pig oocytes by reducing oxidative stress","volume":"88","author":"Gao","year":"2021","journal-title":"Molecular Reproduction and Development"},{"issue":"1","key":"10.1016\/j.vas.2026.100802_bib0007","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0378-8741(01)00175-1","article-title":"Anti-inflammatory activity of lupeol and lupeol linoleate in rats","volume":"76","author":"Geetha","year":"2001","journal-title":"Journal of Ethnopharmacology"},{"issue":"s2","key":"10.1016\/j.vas.2026.100802_bib0008","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/j.1439-0531.2010.01623.x","article-title":"Advances in swine In vitro embryo production technologies","volume":"45","author":"Gil","year":"2010","journal-title":"Reproduction in Domestic Animals"},{"issue":"2","key":"10.1016\/j.vas.2026.100802_bib0009","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0303-7207(01)00683-9","article-title":"Oogenesis as a foundation for embryogenesis","volume":"186","author":"Gosden","year":"2002","journal-title":"Molecular and Cellular Endocrinology"},{"issue":"1","key":"10.1016\/j.vas.2026.100802_bib0010","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0891-5849(93)90126-F","article-title":"Increased generation of reactive oxygen species in embryos cultured in vitro","volume":"15","author":"Goto","year":"1993","journal-title":"Free Radical Biology and Medicine"},{"issue":"2","key":"10.1016\/j.vas.2026.100802_bib0011","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3390\/biochem3020007","article-title":"DMSO alleviates LPS-induced inflammatory responses in RAW264.7 macrophages by inhibiting NF-\u03bab and MAPK activation","volume":"3","author":"Han","year":"2023","journal-title":"BioChem"},{"issue":"3","key":"10.1016\/j.vas.2026.100802_bib0012","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/S0093-691X(99)00014-X","article-title":"Allopurinol, an inhibitor of xanthine oxidase, improves the development of IVM\/IVF bovine embryos (>4 cell) in vitro under certain culture conditions","volume":"51","author":"Iwata","year":"1999","journal-title":"Theriogenology"},{"issue":"1","key":"10.1016\/j.vas.2026.100802_bib0013","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/j.1600-079X.2008.00602.x","article-title":"Effects of melatonin on in vitro maturation of porcine oocyte and expression of melatonin receptor RNA in cumulus and granulosa cells","volume":"46","author":"Kang","year":"2009","journal-title":"Journal of Pineal Research"},{"key":"10.1016\/j.vas.2026.100802_bib0014","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.theriogenology.2017.11.017","article-title":"Lupeol supplementation improves the developmental competence of bovine embryos in vitro","volume":"107","author":"Khan","year":"2018","journal-title":"Theriogenology"},{"issue":"2","key":"10.1016\/j.vas.2026.100802_bib0015","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1095\/biolreprod60.2.336","article-title":"Developmental competence, after transfer to recipients, of porcine oocytes matured, fertilized, and cultured in vitro","volume":"60","author":"Kikuchi","year":"1999","journal-title":"Biology of Reproduction"},{"issue":"10","key":"10.1016\/j.vas.2026.100802_bib0016","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0311819","article-title":"The antioxidant betulinic acid enhances porcine oocyte maturation through Nrf2\/Keap1 signaling pathway modulation","volume":"19","author":"Kim","year":"2024","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.vas.2026.100802_bib0017","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.theriogenology.2012.01.024","article-title":"The effects of resveratrol on porcine oocyte in vitro maturation and subsequent embryonic development after parthenogenetic activation and in vitro fertilization","volume":"78","author":"Kwak","year":"2012","journal-title":"Theriogenology"},{"issue":"2","key":"10.1016\/j.vas.2026.100802_bib0018","doi-asserted-by":"crossref","first-page":"69","DOI":"10.3727\/096504005775082057","article-title":"Expression of estrogen receptor alpha gene in breast cancer cells treated with transcription factor decoy is modulated by Bangladeshi natural plant extracts","volume":"15","author":"Lambertini","year":"2005","journal-title":"Oncology Research"},{"issue":"18","key":"10.1016\/j.vas.2026.100802_bib0019","doi-asserted-by":"crossref","first-page":"8800","DOI":"10.1158\/0008-5472.CAN-07-0801","article-title":"Lupeol suppresses cisplatin-induced nuclear factor-kappaB activation in head and neck squamous cell carcinoma and inhibits local invasion and nodal metastasis in an orthotopic nude mouse model","volume":"67","author":"Lee","year":"2007","journal-title":"Cancer Research"},{"key":"10.1016\/j.vas.2026.100802_bib0020","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.theriogenology.2022.12.015","article-title":"Porcine embryo development and inactivation of microorganisms after ultraviolet-C irradiation at 228 nm","volume":"197","author":"Lin","year":"2023","journal-title":"Theriogenology"},{"issue":"1","key":"10.1016\/j.vas.2026.100802_bib0021","first-page":"5","article-title":"The role of glutathione in mammalian gametes","volume":"5","author":"Luberda","year":"2005","journal-title":"Reproductive Biology"},{"issue":"3","key":"10.1016\/j.vas.2026.100802_bib0022","article-title":"Pentacyclic triterpene bioavailability: An overview of In vitro and In vivo studies","volume":"22","author":"NA","year":"2017","journal-title":"Molecules"},{"issue":"1","key":"10.1016\/j.vas.2026.100802_bib0023","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1186\/s12917-025-05049-4","article-title":"Indole-3-propionic acid supplementation during in vitro maturation improves in vitro production of porcine embryos","volume":"21","author":"Nakayama","year":"2025","journal-title":"BMC Veterinary Research"},{"issue":"3","key":"10.1016\/j.vas.2026.100802_bib0024","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.toxlet.2005.09.001","article-title":"Effects of dimethylsulphoxide on mice arsenite-induced dysmorphogenesis in embryo culture and cytotoxicity in embryo cells","volume":"161","author":"P\u00e9rez-Past\u00e9n","year":"2006","journal-title":"Toxicology Letters"},{"issue":"8","key":"10.1016\/j.vas.2026.100802_bib0025","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1111\/1567-1364.12091","article-title":"Dimethyl sulfoxide induces oxidative stress in the yeast saccharomyces cerevisiae","volume":"13","author":"Sadowska-Bartosz","year":"2013","journal-title":"FEMS Yeast Research"},{"issue":"3","key":"10.1016\/j.vas.2026.100802_bib0026","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1262\/jrd.18124","article-title":"Effects of purple sweet potato anthocyanins on development and intracellular redox status of bovine preimplantation embryos exposed to heat shock","volume":"53","author":"Sakatani","year":"2007","journal-title":"Journal of Reproduction and Development"},{"key":"10.1016\/j.vas.2026.100802_bib0027","doi-asserted-by":"crossref","DOI":"10.1016\/j.theriogenology.2026.117971","article-title":"Effects of in vitro maturation medium supplemented with melatonin on the development and gene expression of deoxynivalenol-exposed porcine oocytes","volume":"261","author":"Shen","year":"2026","journal-title":"Theriogenology"},{"issue":"1","key":"10.1016\/j.vas.2026.100802_bib0028","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0011-2240(82)90125-0","article-title":"Effects of dimethyl sulfoxide on the globally ischemic heart: Possible general relevance to hypothermic organ preservation","volume":"19","author":"Shlafer","year":"1982","journal-title":"Cryobiology"},{"issue":"7","key":"10.1016\/j.vas.2026.100802_bib0029","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.lfs.2010.11.020","article-title":"Beneficial health effects of lupeol triterpene: A review of preclinical studies","volume":"88","author":"Siddique","year":"2011","journal-title":"Life Sciences"},{"issue":"5823","key":"10.1016\/j.vas.2026.100802_bib0030","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1126\/science.1138236","article-title":"Regulation of the oocyte-to-zygote transition","volume":"316","author":"Stitzel","year":"2007","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.vas.2026.100802_bib0031","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1530\/rep.0.1220155","article-title":"Translocation of active mitochondria during pig oocyte maturation, fertilization and early embryo development in vitro","volume":"122","author":"Sun","year":"2001","journal-title":"Reproduction"},{"issue":"5","key":"10.1016\/j.vas.2026.100802_bib0032","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/S0367-326X(01)00259-3","article-title":"Hepatoprotective effect of lupeol and lupeol linoleate on tissue antioxidant defence system in cadmium-induced hepatotoxicity in rats","volume":"72","author":"Sunitha","year":"2001","journal-title":"Fitoterapia"},{"issue":"16","key":"10.1016\/j.vas.2026.100802_bib0033","first-page":"13","article-title":"Melatonin supplementation during in vitro maturation of porcine oocytes alleviates oxidative stress and endoplasmic reticulum stress induced by imidacloprid exposure","author":"Wang","year":"2023","journal-title":"Animals: An Open Access Journal From MDPI"},{"issue":"4","key":"10.1016\/j.vas.2026.100802_bib0034","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1095\/biolreprod60.4.1020","article-title":"Morphologic evaluation and actin filament distribution in porcine embryos produced In vitro and In Vivo1","volume":"60","author":"Wang","year":"1999","journal-title":"Biology of Reproduction"},{"key":"10.1016\/j.vas.2026.100802_bib0035","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.theriogenology.2024.09.018","article-title":"Oleanolic acid promotes porcine oocyte maturation by activating the Nrf2\/HO-1 signalling pathway","volume":"230","author":"Wang","year":"2024","journal-title":"Theriogenology"},{"key":"10.1016\/j.vas.2026.100802_bib0036","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.theriogenology.2020.02.045","article-title":"DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality","volume":"148","author":"Ynsaurralde-Rivolta","year":"2020","journal-title":"Theriogenology"},{"issue":"1","key":"10.1016\/j.vas.2026.100802_bib0037","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1002\/mrd.1080350113","article-title":"Role of glutathione in the maturation and fertilization of pig oocytes in vitro","volume":"35","author":"Yoshida","year":"1993","journal-title":"Molecular Reproduction and Development"},{"issue":"3","key":"10.1016\/j.vas.2026.100802_bib0038","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1262\/jrd.20001","article-title":"Defined system for in vitro production of porcine embryos using a single basic medium","volume":"54","author":"Yoshioka","year":"2008","journal-title":"Journal of Reproduction and Development"}],"container-title":["Veterinary and Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2451943X26002310?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2451943X26002310?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:37:21Z","timestamp":1791909441000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2451943X26002310"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":38,"alternative-id":["S2451943X26002310"],"URL":"https:\/\/doi.org\/10.1016\/j.vas.2026.100802","relation":{},"ISSN":["2451-943X"],"issn-type":[{"value":"2451-943X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Supplementation of lupeol during in vitro maturation improves preimplantation embryonic development in pigs","name":"articletitle","label":"Article Title"},{"value":"Veterinary and Animal Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vas.2026.100802","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"100802"}}