{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:50:54Z","timestamp":1761007854234,"version":"build-2065373602"},"reference-count":31,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmacological and Toxicological Methods"],"published-print":{"date-parts":[[2013,9]]},"DOI":"10.1016\/j.vascn.2013.02.007","type":"journal-article","created":{"date-parts":[[2013,2,28]],"date-time":"2013-02-28T19:16:16Z","timestamp":1362078976000},"page":"269-274","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["In-vitro maintenance and functionality of primary renal tubules and their application in the study of relative renal toxicity of nephrotoxic drugs"],"prefix":"10.1016","volume":"68","author":[{"given":"Jinsheng","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Patton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon K.","family":"Jackson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy M.","family":"Purcell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vascn.2013.02.007_bb0005","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1016\/0006-2952(90)90010-I","article-title":"Primary culture of proximal tubular cells from normal rat kidney as in vitro model to study mechanisms of nephrotoxicicity: Toxicity of nephrotoxicants at low concentrations during prolonged exposure","volume":"39","author":"Boogaard","year":"1990","journal-title":"Biochemistry and Pharmacology"},{"key":"10.1016\/j.vascn.2013.02.007_bb0010","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/0887-2333(89)90032-5","article-title":"Use of monolayers of primary rat kidney cortex cells for nephrotoxicity studies","volume":"3","author":"Bruggeman","year":"1989","journal-title":"Toxicology in Vitro"},{"key":"10.1016\/j.vascn.2013.02.007_bb0015","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/S0090-9556(25)05659-4","article-title":"Ethoxyresorufin: Direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene","volume":"2","author":"Burke","year":"1974","journal-title":"Drug Metabolism and Disposition"},{"key":"10.1016\/j.vascn.2013.02.007_bb0020","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/0160-5402(86)90003-3","article-title":"The use of renal tubule fragments isolated from the rat to investigate aspects of gentamicin nephrotoxicity","volume":"15","author":"Chahwala","year":"1986","journal-title":"Journal of Pharmacological Methods"},{"key":"10.1016\/j.vascn.2013.02.007_bb0025","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1097\/00004032-200401000-00003","article-title":"Model results of kidney burdens from uranium intakes","volume":"86","author":"Chen","year":"2004","journal-title":"Health Physics"},{"key":"10.1016\/j.vascn.2013.02.007_bb0030","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1006\/taap.2000.8994","article-title":"Cytochrome P450-dependent monooxygenase activities and their inducibility by classic P450 inducers in the liver, kidney, and nasal mucosa of male adult ring-necked pheasants","volume":"167","author":"Giorgi","year":"2000","journal-title":"Toxicology and Applied Pharmacology"},{"key":"10.1016\/j.vascn.2013.02.007_bb0035","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/j.tiv.2004.05.001","article-title":"Expression and activities of several drug-metabolizing enzymes in LLC-PK1 cells","volume":"18","author":"Gonzalez","year":"2004","journal-title":"Toxicology In Vitro"},{"key":"10.1016\/j.vascn.2013.02.007_bb0040","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1203\/00006450-199310000-00006","article-title":"Developmental and tissue-specific modulation of rat galactose-1-phosphate uridyltransferase steady state messenger RNA and specific activity levels","volume":"34","author":"Heidenreich","year":"1994","journal-title":"Pediatric Research"},{"year":"1989","series-title":"Basic histology","author":"Junqueira","key":"10.1016\/j.vascn.2013.02.007_bb0045"},{"key":"10.1016\/j.vascn.2013.02.007_bb0050","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1016\/S0006-2952(98)00066-5","article-title":"Role of nitric oxide in the inhibition of cytochrome P450 in the liver of mice infected with Chlamydia trachomatis","volume":"55","author":"Khatsenko","year":"1998","journal-title":"Biochemistry and Pharmacology"},{"key":"10.1016\/j.vascn.2013.02.007_bb0055","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0305-7372(95)90010-1","article-title":"Anticancer drug renal toxicity and elimination: dosing guidelines for altered renal function","volume":"21","author":"Kintzel","year":"1995","journal-title":"Cancer Treatment Reviews"},{"key":"10.1016\/j.vascn.2013.02.007_bb0060","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.tox.2007.07.016","article-title":"Cytotoxicity, metabolism and cellular uptake of the mycotoxin deoxynivalenol in human proximal tubule cells and lung fibroblasts in primary culture","volume":"240","author":"K\u00f6nigs","year":"2007","journal-title":"Toxicology"},{"key":"10.1016\/j.vascn.2013.02.007_bb0065","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1023\/A:1019536115152","article-title":"Effects of cadmium and uranium on some in vitro renal targets","volume":"18","author":"L'Azou","year":"2002","journal-title":"Cell Biology and Toxicology"},{"key":"10.1016\/j.vascn.2013.02.007_bb0070","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0014-5793(98)01283-6","article-title":"Endogenous nitric oxide is responsible for the early loss of P450 in cultured rat hepatocytes","volume":"438","author":"L\u00f3pez-Garc\u00eda","year":"1998","journal-title":"FEBS Letters"},{"key":"10.1016\/j.vascn.2013.02.007_bb0075","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1002\/jcb.10730","article-title":"Biochemical and functional changes of rat liver spheroids during spheroid formation and maintenance in culture: I. Morphological maturation and kinetic changes of energy metabolism, albumin synthesis and activities of some enzymes","volume":"90","author":"Ma","year":"2003","journal-title":"Journal of Cellular Biochemistry"},{"key":"10.1016\/j.vascn.2013.02.007_bb0080","first-page":"1","article-title":"Use of renal tubule suspensions for in vitro toxicity studies","volume":"6","author":"McGuinness","year":"1993","journal-title":"Toxicology in Vitro"},{"key":"10.1016\/j.vascn.2013.02.007_bb0085","first-page":"205","article-title":"Multiplicity and tissue specific expression of camel cytochrome P450(s)","volume":"121","author":"Raza","year":"1998","journal-title":"Comparative Biochemistry and Physiology. C"},{"key":"10.1016\/j.vascn.2013.02.007_bb0090","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/0929-7855(95)00021-H","article-title":"Renal cytochrome P450","volume":"12","author":"Schwartzman","year":"1995","journal-title":"Journal of Lipid Mediators and Cell Signalling"},{"year":"1998","series-title":"Human histology","author":"Stevens","key":"10.1016\/j.vascn.2013.02.007_bb0095"},{"key":"10.1016\/j.vascn.2013.02.007_bb0110","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1054\/mehy.1997.0655","article-title":"Important role of the kidney in human carbohydrate metabolism","volume":"52","author":"Stumvoll","year":"1999","journal-title":"Medical Hypotheses"},{"key":"10.1016\/j.vascn.2013.02.007_bb0105","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1007\/s001250050745","article-title":"Renal glucose production and utilization: New aspects in humans","volume":"40","author":"Stumvoll","year":"1997","journal-title":"Diabetologia"},{"key":"10.1016\/j.vascn.2013.02.007_bb0100","doi-asserted-by":"crossref","first-page":"2528","DOI":"10.1172\/JCI118314","article-title":"Uptake and release of glucose by the human kidney: Postabsorptive rates and responses to epinephrine","volume":"96","author":"Stumvoll","year":"1995","journal-title":"Journal of Clinical Investigation"},{"key":"10.1016\/j.vascn.2013.02.007_bb0115","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ymgme.2011.09.028","article-title":"Innovative therapy for classic galactosemia \u2014 Tale of two HTS","volume":"105","author":"Tang","year":"2012","journal-title":"Molecular Genetics and Metabolism"},{"key":"10.1016\/j.vascn.2013.02.007_bb0145","doi-asserted-by":"crossref","first-page":"861","DOI":"10.2174\/138955709788452577","article-title":"Current considerations in model and high throughput drug safety screening testing","volume":"9","author":"Xu","year":"2009","journal-title":"Mini-Review in Medical Chemistry"},{"key":"10.1016\/j.vascn.2013.02.007_bb0120","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0003-2697(02)00400-1","article-title":"Optimising the enzymatic determination of galactose in the culture media of rat liver and Hepg2 cell spheroids","volume":"311","author":"Xu","year":"2002","journal-title":"Analytical Biochemistry"},{"key":"10.1016\/j.vascn.2013.02.007_bb0125","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1002\/jcb.10731","article-title":"Biochemical and functional changes of rat liver spheroids during spheroid formation and maintenance in culture: II. Nitric oxide synthesis and related changes","volume":"90","author":"Xu","year":"2003","journal-title":"Journal of Cellular Biochemistry"},{"key":"10.1016\/j.vascn.2013.02.007_bb0130","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/S0041-008X(03)00089-9","article-title":"Characterisation of some cytotoxic endpoints using rat liver and Hepg2 spheroids as in vitro models and their application in hepatotoxicity studies: I. glucose metabolism and enzyme release as cytotoxic markers","volume":"189","author":"Xu","year":"2003","journal-title":"Toxicology and Applied Pharmacology"},{"key":"10.1016\/j.vascn.2013.02.007_bb0135","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/S0041-008X(03)00090-5","article-title":"Characterisation of some cytotoxic endpoints using rat liver and Hepg2 spheroids as in vitro models and their application in hepatotoxicity studies: II. Spheroid cell spreading inhibition as a new cytotoxic marker","volume":"189","author":"Xu","year":"2003","journal-title":"Toxicology and Applied Pharmacology"},{"key":"10.1016\/j.vascn.2013.02.007_bb0140","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.taap.2006.05.015","article-title":"Energy metabolism and transformation as endpoints to screen hepatotoxicity using a liver spheroid model","volume":"216","author":"Xu","year":"2006","journal-title":"Toxicology and Applied Pharmacology"},{"key":"10.1016\/j.vascn.2013.02.007_bb0150","doi-asserted-by":"crossref","first-page":"2899","DOI":"10.1016\/j.biomaterials.2009.01.046","article-title":"The impact of extracellular matrix coatings on the performance of human renal cells applied in bioartificial kidneys","volume":"30","author":"Zhang","year":"2009","journal-title":"Biomaterials"},{"key":"10.1016\/j.vascn.2013.02.007_bb0155","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/0012-1606(68)90072-9","article-title":"Gluconeogenesis in mouse kidney cortex: II. Glucose production and enzyme activities in newborn and early postnatal animals","volume":"17","author":"Zorzoli","year":"1968","journal-title":"Developmental Biology"}],"container-title":["Journal of Pharmacological and Toxicological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1056871913002104?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1056871913002104?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T23:57:52Z","timestamp":1761004672000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1056871913002104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["S1056871913002104"],"URL":"https:\/\/doi.org\/10.1016\/j.vascn.2013.02.007","relation":{},"ISSN":["1056-8719"],"issn-type":[{"type":"print","value":"1056-8719"}],"subject":[],"published":{"date-parts":[[2013,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In-vitro maintenance and functionality of primary renal tubules and their application in the study of relative renal toxicity of nephrotoxic drugs","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmacological and Toxicological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vascn.2013.02.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}