{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:54:33Z","timestamp":1761008073595,"version":"build-2065373602"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","doi-asserted-by":"publisher","award":["476060\/2010-9"],"award-info":[{"award-number":["476060\/2010-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","doi-asserted-by":"publisher","award":["E-26\/102.300\/2013"],"award-info":[{"award-number":["E-26\/102.300\/2013"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmacological and Toxicological Methods"],"published-print":{"date-parts":[[2017,3]]},"DOI":"10.1016\/j.vascn.2016.10.009","type":"journal-article","created":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T18:17:24Z","timestamp":1478024244000},"page":"51-56","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Validation of a Na+-shift binding assay for estimation of the intrinsic efficacy of ligands at the A2A adenosine receptor"],"prefix":"10.1016","volume":"84","author":[{"given":"Fran\u00e7ois","family":"No\u00ebl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando M.","family":"do Monte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vascn.2016.10.009_bb0005","doi-asserted-by":"crossref","unstructured":"Alexander, S.P., & Millns, P.J. (2010). [(3)H]ZM241385\u2014An antagonist radioligand for adenosine A(2A) receptors in rat brain. European Journal of Pharmacology, 411, 205\u2013210.","DOI":"10.1016\/S0014-2999(00)00899-2"},{"key":"10.1016\/j.vascn.2016.10.009_bb0010","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1038\/nrd3955","article-title":"Adenosine receptors as drug targets\u2014What are the challenges?","volume":"12","author":"Chen","year":"2013","journal-title":"Nature Reviews Drug Discovery"},{"key":"10.1016\/j.vascn.2016.10.009_bb0015","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1007\/s00018-006-6216-2","article-title":"Heterodimeric adenosine receptors: A device to regulate neurotransmitter release","volume":"63","author":"Ciruela","year":"2006","journal-title":"Cellular and Molecular Life Sciences"},{"key":"10.1016\/j.vascn.2016.10.009_bb0020","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1016\/j.bbamem.2011.02.007","article-title":"Adenosine receptor containing oligomers: Their role in the control of dopamine and glutamate neurotransmission in the brain","volume":"1808","author":"Ciruela","year":"2011","journal-title":"Biochimica et Biophysica Acta"},{"key":"10.1016\/j.vascn.2016.10.009_bb0025","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/BF00168627","article-title":"Evidence for high-affinity binding sites for the adenosine A2A receptor agonist [3H] CGS 21680 in the rat hippocampus and cerebral cortex that are different from striatal A2A receptors","volume":"353","author":"Cunha","year":"1996","journal-title":"Naunyn-Schmiedeberg's Archives of Pharmacology"},{"key":"10.1016\/j.vascn.2016.10.009_bb0030","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0165-6147(89)90266-6","article-title":"Calculating receptor number from binding experiments using same compound as radioligand and competitor","volume":"10","author":"DeBlasi","year":"1989","journal-title":"Trends in Pharmacological Sciences"},{"issue":"1\u20132","key":"10.1016\/j.vascn.2016.10.009_bb4051","first-page":"1","article-title":"Receptor-ligand binding assays: technologies and applications. Journal of Chromatography","volume":"829","author":"de Jong","year":"2015","journal-title":"B: Analytical Technologies in the Biomedical and Life Sciences"},{"key":"10.1016\/j.vascn.2016.10.009_bb0035","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1124\/pr.110.003285","article-title":"International Union of Basic and Clinical Pharmacology. LXXXI. Nomenclature and classification of adenosine receptors\u2014An update","volume":"63","author":"Fredholm","year":"2011","journal-title":"Pharmacological Reviews"},{"key":"10.1016\/j.vascn.2016.10.009_bb0040","doi-asserted-by":"crossref","first-page":"71","DOI":"10.3389\/fendo.2014.00071","article-title":"Diversity and bias through receptor\u2013receptor interactions in GPCR heteroreceptor complexes. Focus on examples from dopamine D2 receptor heteromerization","volume":"5","author":"Fuxe","year":"2014","journal-title":"Frontiers in Endocrinology"},{"key":"10.1016\/j.vascn.2016.10.009_bb0050","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.1111\/j.1476-5381.2012.01897.x","article-title":"Functional efficacy of adenosine A\u2082A receptor agonists is positively correlated to their receptor residence time","volume":"166","author":"Guo","year":"2012","journal-title":"British Journal of Pharmacology"},{"key":"10.1016\/j.vascn.2016.10.009_bb0055","doi-asserted-by":"crossref","first-page":"3577","DOI":"10.1073\/pnas.051632598","article-title":"Most central nervous system D2 dopamine receptors are coupled to their effectors by Go","volume":"98","author":"Jiang","year":"2001","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"10.1016\/j.vascn.2016.10.009_bb0060","doi-asserted-by":"crossref","first-page":"2365","DOI":"10.1016\/0006-2952(92)90681-8","article-title":"Effects of mono- and divalent ions on the binding of the adenosine analogue CGS 21680 to adenosine A2 receptors in rat striatum","volume":"44","author":"Johansson","year":"1992","journal-title":"Biochemical Pharmacology"},{"key":"10.1016\/j.vascn.2016.10.009_bb0065","first-page":"2664","article-title":"Mechanisms of hormone-effector coupling: The B-adrenergic receptor and adenylate cyclase","volume":"41","author":"Lefkowitz","year":"1982","journal-title":"Federation Proceedings"},{"key":"10.1016\/j.vascn.2016.10.009_bb0070","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1126\/science.1219218","article-title":"Structural basis for allosteric regulation of GPCRs by sodium ions","volume":"337","author":"Liu","year":"2012","journal-title":"Science"},{"key":"10.1016\/j.vascn.2016.10.009_bb0075","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin-phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/j.vascn.2016.10.009_bb0085","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0165-6147(02)00027-5","article-title":"Principles: Extending the utility of [35S]GTPgS binding assays","volume":"24","author":"Milligan","year":"2003","journal-title":"Trends in Pharmacological Sciences"},{"key":"10.1016\/j.vascn.2016.10.009_bb0090","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0026-895X(25)15016-5","article-title":"The kinetics of competitive radioligand binding predicted by the Law of Mass Action","volume":"25","author":"Motulsky","year":"1983","journal-title":"Molecular Pharmacology"},{"key":"10.1016\/j.vascn.2016.10.009_bb0095","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1124\/pr.55.4.4","article-title":"International Union of Pharmacology Committee on Receptor Nomenclature and Drug Classification. XXXVIII. Update on terms and symbols in quantitative pharmacology","volume":"55","author":"Neubig","year":"2003","journal-title":"Pharmacological Reviews"},{"key":"10.1016\/j.vascn.2016.10.009_bb0100","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/S0026-895X(25)11023-7","article-title":"Regulation of dopamine D2 receptors by sodium and pH","volume":"39","author":"Neves","year":"1991","journal-title":"Molecular Pharmacology"},{"key":"10.1016\/j.vascn.2016.10.009_bb0105","first-page":"169","article-title":"Radioligand binding assays in the drug discovery process: Potential pitfalls of high throughput screenings","volume":"51","author":"No\u00ebl","year":"2001","journal-title":"Naunyn-Schmiedeberg's Archives of Pharmacology"},{"key":"10.1016\/j.vascn.2016.10.009_bb0110","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.vascn.2014.03.002","article-title":"Functional binding assays for estimation of the intrinsic efficacy of ligands at the 5-HT1A receptor: Application for screening drug candidates","volume":"70","author":"No\u00ebl","year":"2014","journal-title":"Journal of Pharmacological and Toxicological Methods"},{"key":"10.1016\/j.vascn.2016.10.009_bb0115","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1126\/science.182.4119.1359","article-title":"Opiate agonists and antagonists discriminated by receptor binding in brain","volume":"182","author":"Pert","year":"1973","journal-title":"Science"},{"key":"10.1016\/j.vascn.2016.10.009_bb0120","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.ejmech.2013.05.027","article-title":"Synthesis and pharmacological evaluation of new N-phenylpiperazine derivatives designed as homologues of the antipsychotic lead compound LASSBio-579","volume":"66","author":"Pompeu","year":"2013","journal-title":"European Journal of Medicinal Chemistry"},{"key":"10.1016\/j.vascn.2016.10.009_bb0125","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1111\/j.1476-5381.2010.00963.x","article-title":"Use of the GTPgS ([35S]GTPgS and Eu-GTPgS) binding assay for analysis of ligand potency and efficacy at G protein-coupled receptors","volume":"161","author":"Strange","year":"2010","journal-title":"British Journal of Pharmacology"},{"key":"10.1016\/j.vascn.2016.10.009_bb0130","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/s00210-014-1073-2","article-title":"Modulation of GPCRs by monovalent cations and anions","volume":"388","author":"Strasser","year":"2015","journal-title":"Naunyn-Schmiedeberg's Archives of Pharmacology"},{"issue":"7","key":"10.1016\/j.vascn.2016.10.009_bb9000","first-page":"507","article-title":"How were new medicines discovered? Nature Reviews Drug Discovery","volume":"10","author":"Swinney","year":"2011"},{"issue":"13","key":"10.1016\/j.vascn.2016.10.009_bb0135","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1016\/j.lfs.2004.10.027","article-title":"Kinetic and functional properties of [3H]ZM241385, a high affinity antagonist for adenosine A2A receptors","volume":"76","author":"Uustare","year":"2005","journal-title":"Life Sciences"},{"key":"10.1016\/j.vascn.2016.10.009_bb0140","doi-asserted-by":"crossref","first-page":"6641","DOI":"10.1016\/j.bmc.2015.09.012","article-title":"1,3,7-Triethyl-substituted xanthines possess nanomolar affinity for the adenosine A1 receptor","volume":"23","author":"Van der Walt","year":"2015","journal-title":"Bioorganic & Medicinal Chemistry"},{"key":"10.1016\/j.vascn.2016.10.009_bb0145","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1021\/jm970508l","article-title":"5\u2032-substituted adenosine analogs as new high-affinity partial agonists for the adenosine A1 receptor","volume":"41","author":"van der Wenden","year":"1998","journal-title":"Journal of Medicinal Chemistry"},{"key":"10.1016\/j.vascn.2016.10.009_bb0150","doi-asserted-by":"crossref","first-page":"285","DOI":"10.2174\/1386207054020778","article-title":"G-protein coupled receptor assays: To measure affinity or efficacy that is the question","volume":"8","author":"Williams","year":"2005","journal-title":"Combinatorial Chemistry & High Throughput Screening"},{"key":"10.1016\/j.vascn.2016.10.009_bb0155","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1124\/mol.51.6.1060","article-title":"Somatostatin5 receptor-mediated [35S]guanosine-5\u2032-O-(3-thio)triphosphate binding: Agonist potencies and the influence of sodium chloride on intrinsic activity","volume":"51","author":"Williams","year":"1997","journal-title":"Molecular Pharmacology"}],"container-title":["Journal of Pharmacological and Toxicological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1056871916301460?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1056871916301460?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:29Z","timestamp":1761004829000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1056871916301460"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3]]},"references-count":31,"alternative-id":["S1056871916301460"],"URL":"https:\/\/doi.org\/10.1016\/j.vascn.2016.10.009","relation":{},"ISSN":["1056-8719"],"issn-type":[{"type":"print","value":"1056-8719"}],"subject":[],"published":{"date-parts":[[2017,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Validation of a Na+-shift binding assay for estimation of the intrinsic efficacy of ligands at the A2A adenosine receptor","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmacological and Toxicological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vascn.2016.10.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}