{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:06:11Z","timestamp":1761091571230,"version":"build-2065373602"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"School of Pharmacy & Health Professions"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmacological and Toxicological Methods"],"published-print":{"date-parts":[[2019,7]]},"DOI":"10.1016\/j.vascn.2019.106592","type":"journal-article","created":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T16:58:07Z","timestamp":1559235487000},"page":"106592","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Standardization of a new method for assessing the development of cataract in cultured bovine lenses"],"prefix":"10.1016","volume":"98","author":[{"given":"Segewkal","family":"Heruye","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonce N.","family":"Maffofou N.","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neetu U.","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Munt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Fatou","family":"Njie-Mbye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunny E.","family":"Ohia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine A.","family":"Opere","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vascn.2019.106592_bb0005","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/j.1749-6632.2000.tb06877.x","article-title":"The use of explant lens culture to assess cataractogenic potential","volume":"919","author":"Aleo","year":"2000","journal-title":"Annals of the New York Academy of Sciences"},{"key":"10.1016\/j.vascn.2019.106592_bb0010","series-title":"Principle, practices in ophthalmology","first-page":"1428","article-title":"Biochemical mechanisms of age-related cataract","author":"Andley","year":"2002"},{"key":"10.1016\/j.vascn.2019.106592_bb0015","doi-asserted-by":"crossref","first-page":"4076","DOI":"10.1167\/iovs.12-9471","article-title":"Spectral transmission of the human crystalline lens in adult and elderly persons: Color and total transmission of visible light","volume":"53","author":"Artigas","year":"2012","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"10.1016\/j.vascn.2019.106592_bb0020","series-title":"Mechanisms of cataract formation in the human Lens","first-page":"72","article-title":"Protein modification in cataract: Possible oxidative mechanisms","author":"Augusteyn","year":"1981"},{"key":"10.1016\/j.vascn.2019.106592_bb0025","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1080\/02713680802676885","article-title":"Prevention of selenite-induced cataractogenesis by N-acetylcysteine in rats","volume":"34","author":"Aydin","year":"2009","journal-title":"Current Eye Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0030","first-page":"323","article-title":"Hsp70 in bovine lenses during temperature stress","volume":"9","author":"Banh","year":"2003","journal-title":"Molecular Vision"},{"key":"10.1016\/j.vascn.2019.106592_bb0035","first-page":"1911","article-title":"Cataract as a protein condensation disease: The proctor lecture","volume":"38","author":"Benedek","year":"1997","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"10.1016\/j.vascn.2019.106592_bb0040","first-page":"81","article-title":"Assessment of observer variability in the classification of human cataracts","volume":"55","author":"Cicchetti","year":"1982","journal-title":"The Yale Journal of Biology and Medicine"},{"key":"10.1016\/j.vascn.2019.106592_bb0045","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1006\/exer.1993.1111","article-title":"The effect and recovery of long-term H2O2 exposure on lens morphology and biochemistry","volume":"57","author":"Cui","year":"1993","journal-title":"Experimental Eye Research"},{"issue":"1","key":"10.1016\/j.vascn.2019.106592_bb0050","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0041-008X(84)90276-X","article-title":"State of sulfhydryl in selenite cataract","volume":"74","author":"David","year":"1984","journal-title":"Toxicology and Applied Pharmacology"},{"key":"10.1016\/j.vascn.2019.106592_bb0055","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1089\/jop.2000.16.121","article-title":"Glutathione: a vital lens antioxidant","volume":"16","author":"Giblin","year":"2000","journal-title":"Journal of Ocular Pharmacology and Therapeutics"},{"key":"10.1016\/j.vascn.2019.106592_bb0060","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0014-4835(87)80080-5","article-title":"Peroxide-induced effect on lens cation transport following inhibition of glutathione reductase activity in vitro","volume":"45","author":"Giblin","year":"1987","journal-title":"Experimental Eye Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0065","doi-asserted-by":"crossref","first-page":"175","DOI":"10.4103\/0301-4738.49390","article-title":"Advances in pharmacological strategies for the prevention of cataract development","volume":"57","author":"Gupta","year":"2009","journal-title":"Indian Journal of Ophthalmology"},{"key":"10.1016\/j.vascn.2019.106592_bb0070","doi-asserted-by":"crossref","first-page":"3935","DOI":"10.1021\/pr070138h","article-title":"Post-translational modifications in the nuclear region of young, aged, and cataract human lenses","volume":"6","author":"Hains","year":"2007","journal-title":"Journal of Proteome Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0075","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1016\/j.bbapap.2008.07.016","article-title":"Proteomic analysis of the oxidation of cysteine residues in human age-related nuclear cataract lenses","volume":"1784","author":"Hains","year":"2008","journal-title":"Biochimica et Biophysica Acta"},{"issue":"8","key":"10.1016\/j.vascn.2019.106592_bb0080","doi-asserted-by":"crossref","first-page":"4735","DOI":"10.1074\/jbc.274.8.4735","article-title":"Thiolation of the gamma B-crystallins in intact bovine lens exposed to hydrogen peroxide","volume":"274","author":"Hanson","year":"1999","journal-title":"The Journal of Biological Chemistry"},{"key":"10.1016\/j.vascn.2019.106592_bb0085","first-page":"281","article-title":"Prevention of selenite-induced cataract by L-cysteine and vitamin C in rats","volume":"10","author":"Jahadi Hosseini","year":"2008","journal-title":"Iranian Red Crescent Medical Journal"},{"key":"10.1016\/j.vascn.2019.106592_bb0090","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/0014-4835(92)90016-L","article-title":"Ubiquitin and ubiquitin conjugates in human lens","volume":"55","author":"Jahngen-Hodge","year":"1992","journal-title":"Experimental Eye Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0095","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/BF02867672","article-title":"Effect of ketoacids on H2O2-induced cataract","volume":"18","author":"Jain","year":"2003","journal-title":"Indian Journal of Clinical Biochemistry"},{"key":"10.1016\/j.vascn.2019.106592_bb0100","doi-asserted-by":"crossref","first-page":"6762","DOI":"10.1167\/iovs.15-17201","article-title":"Number of people blind or visually impaired by cataract worldwide and in world regions, 1990 to 2010","volume":"56","author":"Khairallah","year":"2015","journal-title":"Investigative Ophthalmology & Visual Science"},{"issue":"7","key":"10.1016\/j.vascn.2019.106592_bb0105","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/S1995-7645(13)60094-2","article-title":"Alpha lipoic acid protects lens from H2O2-induced cataract by inhibiting apoptosis of lens epithelial cells and inducing activation of anti-oxidative enzymes","volume":"6","author":"Li","year":"2013","journal-title":"Asian Pacific Journal of Tropical Medicine"},{"key":"10.1016\/j.vascn.2019.106592_bb0110","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1016\/S1350-9462(03)00050-8","article-title":"Redox regulation in the lens","volume":"22","author":"Lou","year":"2003","journal-title":"Progress in Retinal and Eye Research"},{"issue":"6","key":"10.1016\/j.vascn.2019.106592_bb0115","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/0014-4835(90)90133-F","article-title":"The role of protein-thiol mixed disulfides in cataractogenesis","volume":"50","author":"Lou","year":"1990","journal-title":"Experimental Eye Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0120","doi-asserted-by":"crossref","DOI":"10.1186\/s12886-017-0443-1","article-title":"Prevention and reversal of selenite-induced cataracts by N-acetylcysteine amide in Wistar rats","volume":"17","author":"Maddirala","year":"2017","journal-title":"BMC Ophthalmology"},{"issue":"1568","key":"10.1016\/j.vascn.2019.106592_bb0125","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1098\/rstb.2010.0300","article-title":"The ageing lens and cataract: A model of normal and pathological ageing","volume":"366","author":"Michael","year":"2011","journal-title":"Philosophical Transactions of the Royal Society, B: Biological Sciences"},{"issue":"5","key":"10.1016\/j.vascn.2019.106592_bb0130","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.molmed.2012.03.005","article-title":"Protein Misfolding and aggregation in cataract disease and prospects for prevention","volume":"18","author":"Moreau","year":"2012","journal-title":"Trends in Molecular Medicine"},{"issue":"3","key":"10.1016\/j.vascn.2019.106592_bb0135","doi-asserted-by":"crossref","first-page":"00178","DOI":"10.15406\/aovs.2017.06.00178","article-title":"The pathophysiology of cataract and major interventions to retarding its progression: A mini review","volume":"6","author":"Nartey","year":"2017","journal-title":"Advances in Ophthalmology & Visual System"},{"key":"10.1016\/j.vascn.2019.106592_bb0140","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1136\/bjo.83.9.1064","article-title":"Concentration dependent effects of hydrogen peroxide on lens epithelial cells","volume":"83","author":"Ohguro","year":"1999","journal-title":"British Journal of Ophthalmology"},{"issue":"2","key":"10.1016\/j.vascn.2019.106592_bb0145","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/BF02150636","article-title":"Superoxide dismutase (SOD) in normal and cataractous human lenses","volume":"222","author":"Ohrloff","year":"1984","journal-title":"Graefe's Archive for Clinical and Experimental Ophthalmology"},{"issue":"1","key":"10.1016\/j.vascn.2019.106592_bb0150","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0009-9120(01)00284-3","article-title":"Lens superoxide dismutase and catalase activities in diabetic cataract","volume":"35","author":"\u00d6zmena","year":"2002","journal-title":"Clinical Biochemistry"},{"issue":"2","key":"10.1016\/j.vascn.2019.106592_bb0155","first-page":"11","article-title":"Effect of endogenous antioxidants on hydrogen peroxide induced experimental cataract","volume":"3","author":"Padalkar","year":"2013","journal-title":"International Journal of Health Sciences & Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0160","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1167\/iovs.10-5866","article-title":"Low levels of hydrogen peroxide stimulate corneal epithelial cell adhesion,migration, and wound healing","volume":"52","author":"Pan","year":"2011","journal-title":"Investigative Ophthalmology & Visual Science"},{"issue":"1","key":"10.1016\/j.vascn.2019.106592_bb0165","first-page":"204","article-title":"Attenuation of experimental cataract by vitamin c in rabbits","volume":"30","author":"Peighmbarzadeh","year":"2014","journal-title":"WALIA Journal"},{"key":"10.1016\/j.vascn.2019.106592_bb0170","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1159\/000265449","article-title":"Species survey of glutathione peroxidase and glutathione reductase: Search for an animal model of the human lens","volume":"18","author":"Rathbun","year":"1986","journal-title":"Ophthalmic Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0175","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0014-4835(91)90075-P","article-title":"Age-related cysteine uptake as rate-limiting in glutathione synthesis and glutathione half-life in the cultured human lens","volume":"53","author":"Rathbun","year":"1991","journal-title":"Experimental Eye Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0180","first-page":"166","article-title":"Protective effect of amino acids on eye lenses against oxidative stress induced by hydrogen peroxide","volume":"3","author":"Rathore","year":"2010","journal-title":"Asian Journal of Pharmaceutical and Clinical Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0185","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1111\/j.1749-6632.1989.tb14936.x","article-title":"Vitamin E intake and risk of cataracts in humans","volume":"570","author":"Robertson","year":"1989","journal-title":"Annals of the New York Academy of Sciences"},{"issue":"5","key":"10.1016\/j.vascn.2019.106592_bb0190","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0891-5849(02)01304-7","article-title":"Vitamin C and vitamin E restore the resistance of GSH-depleted lens cells to H2O2","volume":"34","author":"Shang","year":"2003","journal-title":"Free Radical Biology and Medicine"},{"issue":"12","key":"10.1016\/j.vascn.2019.106592_bb0195","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1096\/fasebj.9.12.7672510","article-title":"Oxidative stress-induced cataract: Mechanism of action","volume":"9","author":"Spector","year":"1995","journal-title":"The FASEB Journal"},{"key":"10.1016\/j.vascn.2019.106592_bb0200","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/S0014-4835(81)80107-8","article-title":"Hydrogen peroxide and human cataract","volume":"33","author":"Spector","year":"1981","journal-title":"Experimental Eye Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0205","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/j.exer.2004.12.007","article-title":"Age-related nuclear cataract-oxidation is the key","volume":"80","author":"Truscott","year":"2005","journal-title":"Experimental Eye Research"},{"issue":"4","key":"10.1016\/j.vascn.2019.106592_bb0210","doi-asserted-by":"crossref","first-page":"294","DOI":"10.3109\/15569527.2013.857679","article-title":"Protective effects of N-acetylcysteine on triamcinolone acetonide-induced lens damage in rats","volume":"33","author":"Tuzcu","year":"2014","journal-title":"Cutaneous and Ocular Toxicology"},{"key":"10.1016\/j.vascn.2019.106592_bb0215","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3109\/02713688408997186","article-title":"Oxidative stress on lens and cataract formation: Role of light and oxygen","volume":"3","author":"Varma","year":"1984","journal-title":"Current Eye Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0220","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1016\/0014-4835(86)90091-6","article-title":"Bovine lens neutral proteinase preferentially hydrolyses oxidatively modified glutamine synthetase","volume":"43","author":"Wagner","year":"1986","journal-title":"Experimental Eye Research"},{"key":"10.1016\/j.vascn.2019.106592_bb0225","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/j.1463-5224.2006.00498.x","article-title":"Oxidation, antioxidants and cataract formation: A literature review","volume":"9","author":"Williams","year":"2006","journal-title":"Veterinary Ophthalmology"},{"author":"World Health Organization (WHO)","key":"10.1016\/j.vascn.2019.106592_bb0230"},{"key":"10.1016\/j.vascn.2019.106592_bb0235","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1006\/exer.1994.1009","article-title":"A physiological level of ascorbate inhibits galactose cataract in Guinea pigs by decreasing plyol accumulation in the lens epithelium: A dehydroascorbate-linked mechanism","volume":"58","author":"Yokoyama","year":"1994","journal-title":"Experimental Eye Research"},{"issue":"4","key":"10.1016\/j.vascn.2019.106592_bb0240","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1172\/JCI117514","article-title":"Tamoxifen blocks chloride channels. A possible mechanism for cataract formation","volume":"94","author":"Zhang","year":"1994","journal-title":"Journal of Clinical Investigation"},{"key":"10.1016\/j.vascn.2019.106592_bb0245","first-page":"862","article-title":"Effects of N-acetylcysteine and glutathione ethyl ester drops on streptozotocin-induced diabetic cataract in rats","volume":"14","author":"Zhang","year":"2008","journal-title":"Molecular Vision"},{"issue":"10","key":"10.1016\/j.vascn.2019.106592_bb0250","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/S0891-5849(03)00505-7","article-title":"Tempol-H inhibits opacification of lenses in organ culture","volume":"35","author":"Zigler","year":"2003","journal-title":"Free Radical Biology & Medicine"}],"container-title":["Journal of Pharmacological and Toxicological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1056871919300772?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1056871919300772?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:02:53Z","timestamp":1761004973000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1056871919300772"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7]]},"references-count":50,"alternative-id":["S1056871919300772"],"URL":"https:\/\/doi.org\/10.1016\/j.vascn.2019.106592","relation":{},"ISSN":["1056-8719"],"issn-type":[{"type":"print","value":"1056-8719"}],"subject":[],"published":{"date-parts":[[2019,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Standardization of a new method for assessing the development of cataract in cultured bovine lenses","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmacological and Toxicological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vascn.2019.106592","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"106592"}}