{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:11:21Z","timestamp":1785370281127,"version":"3.55.0"},"reference-count":18,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmacological and Toxicological Methods"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1016\/j.vascn.2022.107186","type":"journal-article","created":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T12:32:17Z","timestamp":1653568337000},"page":"107186","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Development and validation of a LC-MS\/MS method for simultaneous quantification of Ivabradine and Metoprolol in rat plasma"],"prefix":"10.1016","volume":"116","author":[{"given":"M. Mukkanti","family":"Eswarudu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. Lakshmana","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Vijay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vascn.2022.107186_bb0005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/chir.22102","article-title":"Stereo selective Determination of Metoprolol and its Metabolite \u03b1-Hydroxy metoprolol in Plasma by LC-MS\/MS: Application to Pharmacokinetics during Pregnancy","volume":"25","author":"Antunes","year":"2012","journal-title":"Chirality."},{"key":"10.1016\/j.vascn.2022.107186_bb0010","series-title":"Department of health and human services, food and drug administration, Rockville, MD, USA","first-page":"1","author":"Bioanalytical Method Validation","year":"2018"},{"key":"10.1016\/j.vascn.2022.107186_bb0015","series-title":"Bioanalytical Method Validation - Guidance for Industry","first-page":"1","year":"2018"},{"issue":"2","key":"10.1016\/j.vascn.2022.107186_bb0020","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/S0378-4347(00)00275-9","article-title":"Simultaneous determination of Ivabradine and its metabolites in human plasma by liquid chromatography-tandem mass spectroscopy","volume":"745","author":"Bouchard","year":"2000","journal-title":"Journal of Chromatography. Biomedical Sciences and Applications."},{"issue":"2","key":"10.1016\/j.vascn.2022.107186_bb0025","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.apsb.2012.01.004","article-title":"Simultaneous determination of Ivabradine and N-desmethyl Ivabradine in human plasma and urine using a LC-MS\/MS method: Application to a pharmacokinetic study","volume":"2","author":"Chengtao","year":"2012","journal-title":"Acta Pharmaceutica Sinica B"},{"issue":"5","key":"10.1016\/j.vascn.2022.107186_bb0030","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/nrcardio.2009.47","article-title":"Angina: Ivabradine for treatment of stable angina pectoris","volume":"6","author":"De Silva","year":"2009","journal-title":"Nature Reviews. Cardiology"},{"issue":"4","key":"10.1016\/j.vascn.2022.107186_bb0035","doi-asserted-by":"crossref","first-page":"313","DOI":"10.2174\/187152506778520709","article-title":"Heart rate lowering by inhibition of the pacemaker current: A new therapeutic perspective in cardiovascular disease","volume":"4","author":"Dilaveris","year":"2006","journal-title":"Cardiovascular & Hematological Agents in Medicinal Chemistry"},{"key":"10.1016\/j.vascn.2022.107186_bb0040","series-title":"Guideline on Bioanalytical Method Validation, EMEA\/CHMP\/EWP\/192217\/2009","author":"EMA","year":"2011"},{"issue":"7","key":"10.1016\/j.vascn.2022.107186_bb0045","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1185\/030079905X50543","article-title":"Cardiac pacemaker I(f) current and its inhibition by heart rate-reducing agents","volume":"21","author":"Francesco","year":"2005","journal-title":"Current Medical Research and Opinion"},{"issue":"12","key":"10.1016\/j.vascn.2022.107186_bb0050","first-page":"79","article-title":"Evolution of oral drug forms of metoprolol: Advantages of long-acting modified release forms with modified release","volume":"50","author":"Leonova","year":"2010","journal-title":"Kardiologiia."},{"issue":"3","key":"10.1016\/j.vascn.2022.107186_bb0055","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.3390\/molecules17032663","article-title":"Rapid and sensitive LC-MS\/MS method for the determination of metoprolol in beagle dog plasma with a simple protein precipitation treatment and its pharmacokinetic applications","volume":"17","author":"Li","year":"2012","journal-title":"Molecules."},{"issue":"1","key":"10.1016\/j.vascn.2022.107186_bb0060","first-page":"1","article-title":"Bioanalytical method development and validation for simultaneous determination of chlorthalidone and cilnidipine drugs in human plasma by RP-HPLC","volume":"9","author":"Mukkanti Eswarudu","year":"2019","journal-title":"International Journal of Research in Pharmacy and Chemistry"},{"key":"10.1016\/j.vascn.2022.107186_bb0065","series-title":"The Merck Index: An Encyclopaedia of Chemicals, Drugs and Biologicals","volume":"90","author":"O\u2019Neil","year":"2006"},{"issue":"12","key":"10.1016\/j.vascn.2022.107186_bb0070","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1038\/nrd3576","article-title":"Exploring HCN channels as novel drug targets. Nature reviews","volume":"10","author":"Postea","year":"2011","journal-title":"Drug discovery"},{"issue":"3","key":"10.1016\/j.vascn.2022.107186_bb0075","doi-asserted-by":"crossref","first-page":"49","DOI":"10.17145\/jab.17.008","article-title":"A validated high-resolution accurate mass LC-MS assay for quantitative determination of metoprolol and \u03b1-hydroxy metoprolol in human serum for application in pharmacokinetics","volume":"3","author":"Postma-Kunnen","year":"2017","journal-title":"Journal of applied Bioanalysis"},{"key":"10.1016\/j.vascn.2022.107186_bb0080","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jchromb.2013.01.016","article-title":"A validated, rapid UPLC\u2013MS\/MS method for simultaneous Ivabradine, Reboxetine, and metoprolol analysis in human plasma and its application to clinical trial samples","volume":"927","author":"Zoerner","year":"2013","journal-title":"Journal of Chromatography B"},{"key":"10.1016\/j.vascn.2022.107186_bb0085","doi-asserted-by":"crossref","first-page":"8459","DOI":"10.1039\/C5AY01657F","article-title":"Simultaneous determination of Ivabradine, metoprolol and their metabolites in rat plasma by ultra-performance liquid chromatography tandem mass spectrometry and its application in a pharmacokinetic study","volume":"7","author":"Sun","year":"2015","journal-title":"Analytical Methods"},{"key":"10.1016\/j.vascn.2022.107186_bb0090","first-page":"543","article-title":"Quantitative determination of metoprolol by liquid chromatography mass spectrometry in rat plasma","volume":"33","author":"Zhang","year":"2009","journal-title":"Bulletin of the Academy of Military Medical Sciences."}],"container-title":["Journal of Pharmacological and Toxicological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1056871922000338?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1056871922000338?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:05:09Z","timestamp":1761005109000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1056871922000338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":18,"alternative-id":["S1056871922000338"],"URL":"https:\/\/doi.org\/10.1016\/j.vascn.2022.107186","relation":{},"ISSN":["1056-8719"],"issn-type":[{"value":"1056-8719","type":"print"}],"subject":[],"published":{"date-parts":[[2022,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and validation of a LC-MS\/MS method for simultaneous quantification of Ivabradine and Metoprolol in rat plasma","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmacological and Toxicological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vascn.2022.107186","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"107186"}}