{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T10:23:19Z","timestamp":1781173399299,"version":"3.54.1"},"reference-count":41,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"National Basic Research and Development Program of China","award":["2012CB518805"],"award-info":[{"award-number":["2012CB518805"]}]},{"name":"National Spark Program","award":["2014GA690017"],"award-info":[{"award-number":["2014GA690017"]}]},{"name":"Research and Development Program of Jiangsu","award":["BE2015343"],"award-info":[{"award-number":["BE2015343"]}]},{"name":"NSF of Yangzhou","award":["YZ2014027"],"award-info":[{"award-number":["YZ2014027"]}]},{"name":"Priority Academic Development Program of Jiangsu Higher Education Institutions"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Immunology and Immunopathology"],"published-print":{"date-parts":[[2015,11]]},"DOI":"10.1016\/j.vetimm.2015.09.001","type":"journal-article","created":{"date-parts":[[2015,9,7]],"date-time":"2015-09-07T14:01:32Z","timestamp":1441634492000},"page":"111-117","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Screening putative antigens as stimulators in the Mycobacterium bovis interferon-gamma release assay for cattle"],"prefix":"10.1016","volume":"168","author":[{"given":"Chuang","family":"Meng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengzhong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fa","family":"Shan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuelan","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinan","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetimm.2015.09.001_bib0005","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.prevetmed.2010.06.007","article-title":"Detection of bovine tuberculosis in herds with different disease prevalence and influence of paratuberculosis infection on PPDB and ESAT-6\/CFP10 specificity","volume":"96","author":"Aagaard","year":"2010","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0010","doi-asserted-by":"crossref","first-page":"4326","DOI":"10.1128\/JCM.01184-06","article-title":"Optimizing antigen cocktails for detection of Mycobacterium bovis in herds with different prevalences of bovine tuberculosis: ESAT6-CFP10 mixture shows optimal sensitivity and specificity","volume":"44","author":"Aagaard","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0015","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.3201\/eid1608.100314","article-title":"Novel Mycobacterium tuberculosis complex pathogen M. mungi","volume":"16","author":"Alexander","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0020","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.vetmic.2008.09.060","article-title":"Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay","volume":"135","author":"Alvarez","year":"2009","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0025","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/S0140-6736(00)02742-2","article-title":"Specific immune-based diagnosis of tuberculosis","volume":"356","author":"Andersen","year":"2000","journal-title":"Lancet"},{"key":"10.1016\/j.vetimm.2015.09.001_bib0030","series-title":"Bovine Tuberculosis, Diagnostic Techniques. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 2.4.7","first-page":"686","author":"Anon","year":"2009"},{"key":"10.1016\/j.vetimm.2015.09.001_bib0035","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1051\/vetres:2006021","article-title":"Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis","volume":"37","author":"Aranaz","year":"2006","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0040","first-page":"924","article-title":"Bovine tuberculosis: an old disease but a new threat to Africa","volume":"8","author":"Ayele","year":"2004","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0045","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1128\/CVI.00519-13","article-title":"Clinical and diagnostic developments of a gamma interferon release assay for use in bovine tuberculosis control programs","volume":"20","author":"Bass","year":"2013","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0050","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1126\/science.284.5419.1520","article-title":"Comparative genomics of BCG vaccines by whole-genome DNA microarray","volume":"284","author":"Behr","year":"1999","journal-title":"Science"},{"key":"10.1016\/j.vetimm.2015.09.001_bib0055","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1186\/1471-2334-10-273","article-title":"Rv1985c, a promising novel antigen for diagnosis of tuberculosis infection from BCG-vaccinated controls","volume":"10","author":"Chen","year":"2010","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0060","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1128\/CVI.00209-06","article-title":"Field evaluation of a novel differential diagnostic reagent for detection of Mycobacterium bovis in cattle","volume":"13","author":"Cockle","year":"2006","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0065","doi-asserted-by":"crossref","first-page":"59","DOI":"10.3201\/eid0401.980108","article-title":"Zoonotic tuberculosis due to Mycobacterium bovis in developing countries","volume":"4","author":"Cosivi","year":"1998","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0070","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.rvsc.2005.11.005","article-title":"Ante mortem diagnosis of tuberculosis in cattle: a review of the tuberculin tests, gamma-interferon assay and other ancillary diagnostic techniques","volume":"81","author":"de la Rua-Domenech","year":"2006","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0075","doi-asserted-by":"crossref","first-page":"e52851","DOI":"10.1371\/journal.pone.0052851","article-title":"High prevalence of bovine tuberculosis in dairy cattle in central ethiopia: implications for the dairy industry and public health","volume":"7","author":"Firdessa","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.vetimm.2015.09.001_bib0080","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1128\/CVI.00486-08","article-title":"An improved whole-blood gamma interferon assay based on the CFP21-MPT64 fusion protein","volume":"16","author":"Fu","year":"2009","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0085","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.vetimm.2014.03.015","article-title":"Agreement between assays of cell-mediated immunity utilizing Mycobacterium bovis-specific antigens for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer)","volume":"160","author":"Goosen","year":"2014","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0090","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.vetimm.2004.08.002","article-title":"The effect of the tuberculin test and the consequences of a delay in blood culture on the sensitivity of a gamma-interferon assay for the detection of Mycobacterium bovis infection in cattle","volume":"102","author":"Gormley","year":"2004","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0095","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.diagmicrobio.2013.12.018","article-title":"Utility of B-cell epitopes based peptides of RD1 and RD2 antigens for immunodiagnosis of pulmonary tuberculosis","volume":"78","author":"Goyal","year":"2014","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0100","doi-asserted-by":"crossref","first-page":"e5","DOI":"10.1371\/journal.ppat.0010005","article-title":"Ancient origin and gene mosaicism of the progenitor of Mycobacterium tuberculosis","volume":"1","author":"Gutierrez","year":"2005","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0105","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.1016\/j.vaccine.2012.01.048","article-title":"A recombinant adenovirus expressing immunodominant TB antigens can significantly enhance BCG-induced human immunity","volume":"30","author":"Hoft","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vetimm.2015.09.001_bib0110","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1111\/j.1365-2249.2005.02882.x","article-title":"Exposure to Mycobacterium avium induces low-level protection from Mycobacterium bovis infection but compromises diagnosis of disease in cattle","volume":"141","author":"Hope","year":"2005","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0115","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.diagmicrobio.2009.09.005","article-title":"Utility of a combination of RD1 and RD2 antigens as a diagnostic marker for tuberculosis","volume":"66","author":"Kalra","year":"2010","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0120","doi-asserted-by":"crossref","first-page":"e34991","DOI":"10.1371\/journal.pone.0034991","article-title":"The secreted lipoprotein, MPT83, of Mycobacterium tuberculosis is recognized during human tuberculosis and stimulates protective immunity in mice","volume":"7","author":"Kao","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.vetimm.2015.09.001_bib0125","doi-asserted-by":"crossref","first-page":"463","DOI":"10.4102\/ojvr.v79i2.463","article-title":"Bovine tuberculosis at the human\u2013livestock\u2013wildlife interface: is it a public health problem in Tanzania? A review","volume":"79","author":"Katale","year":"2012","journal-title":"Onderstepoort J. Vet. Res."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0130","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1128\/jb.178.5.1274-1282.1996","article-title":"Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis","volume":"178","author":"Mahairas","year":"1996","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0135","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.cimid.2009.08.003","article-title":"The use of MPB70 and MPB83 to distinguish between bovine tuberculosis and paratuberculosis","volume":"33","author":"Marassi","year":"2010","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0140","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.vetmic.2009.08.029","article-title":"Mycobacterium bovis at the animal\u2013human interface: a problem, or not?","volume":"140","author":"Michel","year":"2010","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0145","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1365-3083.2008.02221.x","article-title":"Th1 cell reactivity and HLA-DR binding prediction for promiscuous recognition of MPT63 (Rv1926c), a major secreted protein of Mycobacterium tuberculosis","volume":"69","author":"Mustafa","year":"2009","journal-title":"Scand. J. Immunol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0150","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1111\/j.1365-3083.2009.02312.x","article-title":"Regions of differences encoded antigens as targets for immunodiagnosis of tuberculosis in humans","volume":"70","author":"Parkash","year":"2009","journal-title":"Scand. J. Immunol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0155","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1053\/tvjl.2001.0655","article-title":"Mycobacterium bovis infection and tuberculosis in cattle","volume":"163","author":"Pollock","year":"2002","journal-title":"Vet. J."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0160","first-page":"205","article-title":"Bovine tuberculosis: a review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication","volume":"57","author":"Schiller","year":"2010","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0165","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1136\/vr.c3403","article-title":"Comparison of tuberculin activity in the interferon-gamma assay for the diagnosis of bovine tuberculosis","volume":"167","author":"Schiller","year":"2010","journal-title":"Vet. Rec."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0170","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1128\/CVI.00150-09","article-title":"Optimization of a whole-blood gamma interferon assay for detection of Mycobacterium bovis-infected cattle","volume":"16","author":"Schiller","year":"2009","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0175","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1111\/j.1365-3083.2009.02325.x","article-title":"New alternative vaccine component against Mycobacterium tuberculosis \u2013 heat shock protein 16.3 or its T-cell epitope","volume":"70","author":"Shi","year":"2009","journal-title":"Scand. J. Immunol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0180","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1128\/CDLI.7.2.155-160.2000","article-title":"Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP10","volume":"7","author":"van Pinxteren","year":"2000","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0185","first-page":"38","article-title":"The scientific case for the gamma interferon BOVIGAM assay","volume":"19","author":"Vordermeier","year":"2008","journal-title":"Gov. Vet. J."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0190","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1128\/CVI.00481-12","article-title":"Mycobacterium tuberculosis region of difference (RD) 2 antigen Rv1985c and RD11 antigen Rv3425 have the promising potential to distinguish patients with active tuberculosis from M. bovis BCG-vaccinated individuals","volume":"20","author":"Wang","year":"2013","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0195","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/S1286-4579(02)01562-9","article-title":"Control of Mycobacterium bovis infections and the risk to human populations","volume":"4","author":"Wedlock","year":"2002","journal-title":"Microbes Infect."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0200","doi-asserted-by":"crossref","first-page":"3176","DOI":"10.1128\/JCM.00420-10","article-title":"Development of a skin test for bovine tuberculosis for differentiating infected from vaccinated animals","volume":"48","author":"Whelan","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetimm.2015.09.001_bib0205","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1054\/tube.2000.0272","article-title":"BOVIGAM: an in vitro cellular diagnostic test for bovine tuberculosis","volume":"81","author":"Wood","year":"2001","journal-title":"Tuberculosis (Edinb.)"}],"container-title":["Veterinary Immunology and Immunopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165242715001981?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165242715001981?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T07:23:29Z","timestamp":1759044209000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0165242715001981"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11]]},"references-count":41,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["S0165242715001981"],"URL":"https:\/\/doi.org\/10.1016\/j.vetimm.2015.09.001","relation":{},"ISSN":["0165-2427"],"issn-type":[{"value":"0165-2427","type":"print"}],"subject":[],"published":{"date-parts":[[2015,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Screening putative antigens as stimulators in the Mycobacterium bovis interferon-gamma release assay for cattle","name":"articletitle","label":"Article Title"},{"value":"Veterinary Immunology and Immunopathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetimm.2015.09.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}