{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T13:28:41Z","timestamp":1765546121110,"version":"3.40.5"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Baoqing Guo for rescuing rJXwn06 virus from the infectious clone cDNA; Jason Crabtree and Jason Huegel for animal care and handling; Bruce Pesch for flow cytometry and David Michael"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Immunology and Immunopathology"],"published-print":{"date-parts":[[2016,10]]},"DOI":"10.1016\/j.vetimm.2016.08.007","type":"journal-article","created":{"date-parts":[[2016,8,11]],"date-time":"2016-08-11T06:40:39Z","timestamp":1470897639000},"page":"70-76","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Enhancement of innate immunity with granulocyte colony-stimulating factor did not mitigate disease in pigs infected with a highly pathogenic Chinese PRRSV strain"],"prefix":"10.1016","volume":"179","author":[{"given":"Sarah N.","family":"Schlink","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly M.","family":"Lager","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan L.","family":"Brockmeier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Crystal L.","family":"Loving","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8946-9416","authenticated-orcid":false,"given":"Laura C.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann C.","family":"Vorwald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han-Chun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"Marcus E.","family":"Kehrli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kay S.","family":"Faaberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetimm.2016.08.007_bib0005","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.3201\/eid1709.110411","article-title":"Highly pathogenic porcine reproductive and respiratory syndrome virus, Asia","volume":"17","author":"An","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0010","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.cimid.2010.06.004","article-title":"Cytokine expression, glucocorticoid and growth hormone changes after porcine reproductive and respiratory syndrome virus (PRRSV-1) infection in vaccinated and unvaccinated naturally exposed pigs","volume":"34","author":"Borghetti","year":"2011","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0015","doi-asserted-by":"crossref","first-page":"521","DOI":"10.2460\/ajvr.2001.62.521","article-title":"Effects of intranasal inoculation with Bordetella bronchiseptica, porcine reproductive and respiratory syndrome virus, or a combination of both organisms on subsequent infection with Pasteurella multocida in pigs","volume":"62","author":"Brockmeier","year":"2001","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0020","doi-asserted-by":"crossref","first-page":"2484","DOI":"10.1073\/pnas.84.8.2484","article-title":"In vivo stimulation of granulopoiesis by recombinant human granulocyte colony-stimulating factor","volume":"84","author":"Cohen","year":"1987","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0025","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1093\/annonc\/mdg019","article-title":"A randomized double-blind multicenter phase III study of fixed-dose single-administration pegfilgrastim versus daily filgrastim in patients receiving myelosuppressive chemotherapy","volume":"14","author":"Green","year":"2003","journal-title":"Ann. Oncol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0030","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.virol.2012.09.013","article-title":"Experimental infection of United States swine with a Chinese highly pathogenic strain of porcine reproductive and respiratory syndrome virus","volume":"435","author":"Guo","year":"2013","journal-title":"Virology"},{"key":"10.1016\/j.vetimm.2016.08.007_bib0035","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.virol.2013.08.008","article-title":"Chinese and Vietnamese strains of HP-PRRSV cause different pathogenic outcomes in United States high health swine","volume":"446","author":"Guo","year":"2013","journal-title":"Virology"},{"key":"10.1016\/j.vetimm.2016.08.007_bib0040","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1136\/vr.151.12.344","article-title":"Effects of different us isolates of porcine reproductive and respiratory syndrome virus (PRRSV) on blood and bone marrow parameters of experimentally infected pigs","volume":"151","author":"Halbur","year":"2002","journal-title":"Vet. Rec."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0045","doi-asserted-by":"crossref","first-page":"231","DOI":"10.2460\/ajvr.76.3.231","article-title":"Efficacy of polyethylene glycol-conjugated bovine granulocyte colony-stimulating factor for reducing the incidence of naturally occurring clinical mastitis in periparturient dairy cows and heifers","volume":"76","author":"Hassfurther","year":"2015","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0050","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1200\/JCO.2002.20.3.727","article-title":"Blinded, randomized, multicenter study to evaluate single administration pegfilgrastim once per cycle versus daily filgrastim as an adjunct to chemotherapy in patients with high-risk stage II or stage III\/IV breast cancer","volume":"20","author":"Holmes","year":"2002","journal-title":"J. Clin. Oncol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0055","first-page":"72","article-title":"Assessment of the economic impact of porcine reproductive and respiratory syndrome virus on United States pork producers","volume":"21","author":"Holtkamp","year":"2013","journal-title":"J. Swine Health Prod."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0060","doi-asserted-by":"crossref","first-page":"4399","DOI":"10.3168\/jds.S0022-0302(91)78636-0","article-title":"Immunobiology of hematopoietic colony-stimulatory factors: potential application to disease prevention in the bovine","volume":"74","author":"Kehrli","year":"1991","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0065","doi-asserted-by":"crossref","first-page":"2448","DOI":"10.3168\/jds.S0022-0302(91)78420-8","article-title":"Effects of granulocyte colony-stimulating factor administration to periparturient cows on neutrophils and bacterial shedding","volume":"74","author":"Kehrli","year":"1991","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0070","doi-asserted-by":"crossref","DOI":"10.3791\/51301","article-title":"Bioenergetics and the oxidative burst: protocols for the isolation and evaluation of human leukocytes and platelets","author":"Kramer","year":"2014","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0075","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.tvjl.2007.07.032","article-title":"Emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus in the Mid-Eastern region of China","volume":"174","author":"Li","year":"2007","journal-title":"Vet. J."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0080","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jcpa.2015.04.002","article-title":"Comparative respiratory pathogenicity and dynamic tissue distribution of chinese highly pathogenic porcine reproductive and respiratory syndrome virus and its attenuated strain in piglets","volume":"153","author":"Liu","year":"2015","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0085","first-page":"2085","article-title":"Kinetics of neutrophil production in normal and neutropenic animals during the response to filgrastim (r-metHu G-CSF) or filgrastim SD\/01 (PEG-r-metHu G-CSF)","volume":"7","author":"Lord","year":"2001","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0090","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.biologicals.2013.07.001","article-title":"Porcine granulocyte-colony stimulating factor (G-CSF) delivered via replication-defective adenovirus induces a sustained increase in circulating peripheral blood neutrophils","volume":"41","author":"Loving","year":"2013","journal-title":"Biologicals"},{"key":"10.1016\/j.vetimm.2016.08.007_bib0095","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1099\/vir.0.2008\/001529-0","article-title":"An infectious cDNA clone of a highly pathogenic porcine reproductive and respiratory syndrome virus variant associated with porcine high fever syndrome","volume":"89","author":"Lv","year":"2008","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0100","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1038\/ni.2921","article-title":"Neutrophils at work","volume":"15","author":"Nauseef","year":"2014","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0105","doi-asserted-by":"crossref","first-page":"3286","DOI":"10.3168\/jds.S0022-0302(89)79490-X","article-title":"Effects of recombinant granulocyte colony-stimulating factor on Staphylococcus aureus mastitis in lactating dairy cows","volume":"72","author":"Nickerson","year":"1989","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0110","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.dci.2006.06.002","article-title":"Phenotypic and functional characterization of porcine granulocyte developmental stages using two new markers","volume":"31","author":"Perez","year":"2007","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0115","doi-asserted-by":"crossref","first-page":"9538","DOI":"10.1128\/JVI.00177-13","article-title":"An intact sialoadhesin (Sn\/SIGLEC1\/CD169) is not required for attachment\/internalization of the porcine reproductive and respiratory syndrome virus","volume":"87","author":"Prather","year":"2013","journal-title":"J. Virol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0120","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1177\/104063879400600102","article-title":"Experimental porcine reproductive and respiratory syndrome virus infection in one- four-, and 10-week-old pigs","volume":"6","author":"Rossow","year":"1994","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0125","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1038\/nbt725","article-title":"Rational cytokine design for increased lifetime and enhanced potency using pH-activated histidine switching","volume":"20","author":"Sarkar","year":"2002","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0130","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1089\/088282403322396154","article-title":"Interleukin-10, interleukin-12, and interferon-gamma levels in the respiratory tract following mycoplasma hyopneumoniae and PRRSV infection in pigs","volume":"16","author":"Thanawongnuwech","year":"2003","journal-title":"Viral Immunol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0135","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0165-2427(97)00078-0","article-title":"Effect of porcine reproductive and respiratory syndrome virus (PRRSV) (isolate ATCC VR-2385) infection on bactericidal activity of porcine pulmonary intravascular macrophages (PIMs): in vitro comparisons with pulmonary alveolar macrophages (PAMs)","volume":"59","author":"Thanawongnuwech","year":"1997","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0140","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0165-2427(01)00243-4","article-title":"Differential production of proinflammatory cytokines: in vitro PRRSV and Mycoplasma hyopneumoniae co-infection model","volume":"79","author":"Thanawongnuwech","year":"2001","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0145","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.vetmic.2004.03.005","article-title":"Genetics and geographical variation of porcine reproductive and respiratory syndrome virus (PRRSV) in Thailand","volume":"101","author":"Thanawongnuwech","year":"2004","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0150","doi-asserted-by":"crossref","first-page":"e526","DOI":"10.1371\/journal.pone.0000526","article-title":"Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark","volume":"2","author":"Tian","year":"2007","journal-title":"PLoS One"},{"key":"10.1016\/j.vetimm.2016.08.007_bib0155","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.3201\/eid1309.070399","article-title":"Highly pathogenic porcine reproductive and respiratory syndrome","volume":"13","author":"Tong","year":"2007","journal-title":"China. Emerg. Infect. Dis."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0160","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.prevetmed.2014.08.009","article-title":"Emergence of highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) in medium-scale swine farms in southeastern Cambodia","volume":"118","author":"Tornimbene","year":"2015","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0165","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.virusres.2015.07.004","article-title":"Emergence of a virulent porcine reproductive and respiratory syndrome virus in vaccinated herds in the United States","volume":"210","author":"Wang","year":"2015","journal-title":"Virus Res."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0170","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1084\/jem.165.4.941","article-title":"Recombinant human granulocyte colony-stimulating factor. Effects on hematopoiesis in normal and cyclophosphamide-treated primates","volume":"165","author":"Welte","year":"1987","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0175","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1186\/1743-422X-7-184","article-title":"Secondary infection with Streptococcus suis serotype 7 increases the virulence of highly pathogenic porcine reproductive and respiratory syndrome virus in pigs","volume":"7","author":"Xu","year":"2010","journal-title":"Virol. J."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0180","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1354\/vp.41-6-649","article-title":"Effect of bovine granulocyte colony-stimulating factor on the development of pneumonia caused by Mannheimia haemolytica","volume":"41","author":"Youssef","year":"2004","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0185","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1172\/JCI107591","article-title":"Human lymphocyte subpopulations. Effect of corticosteroids","volume":"53","author":"Yu","year":"1974","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.vetimm.2016.08.007_bib0190","doi-asserted-by":"crossref","first-page":"5156","DOI":"10.1128\/JVI.02678-08","article-title":"The 30-amino-acid deletion in the nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence","volume":"83","author":"Zhou","year":"2009","journal-title":"J. Virol."}],"container-title":["Veterinary Immunology and Immunopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165242716301532?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165242716301532?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,9]],"date-time":"2018-09-09T04:56:13Z","timestamp":1536468973000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0165242716301532"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":38,"alternative-id":["S0165242716301532"],"URL":"https:\/\/doi.org\/10.1016\/j.vetimm.2016.08.007","relation":{},"ISSN":["0165-2427"],"issn-type":[{"type":"print","value":"0165-2427"}],"subject":[],"published":{"date-parts":[[2016,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Enhancement of innate immunity with granulocyte colony-stimulating factor did not mitigate disease in pigs infected with a highly pathogenic Chinese PRRSV strain","name":"articletitle","label":"Article Title"},{"value":"Veterinary Immunology and Immunopathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetimm.2016.08.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}