{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,14]],"date-time":"2026-11-14T01:15:29Z","timestamp":1794618929870,"version":"4.9.1"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:00:00Z","timestamp":1538352000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:00:00Z","timestamp":1538352000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2018,10,10]],"date-time":"2018-10-10T00:00:00Z","timestamp":1539129600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Immunology and Immunopathology"],"published-print":{"date-parts":[[2018,10]]},"DOI":"10.1016\/j.vetimm.2018.10.001","type":"journal-article","created":{"date-parts":[[2018,10,11]],"date-time":"2018-10-11T13:35:09Z","timestamp":1539264909000},"page":"52-58","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":34,"special_numbering":"C","title":["Detection of chicken interleukin-10 production in intestinal epithelial cells and necrotic enteritis induced by Clostridium perfringens using capture ELISA"],"prefix":"10.1016","volume":"204","author":[{"given":"Youngsub","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6874-6787","authenticated-orcid":false,"given":"Woo H.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-jin","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun S.","family":"Lillehoj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetimm.2018.10.001_bib0005","doi-asserted-by":"crossref","first-page":"430","DOI":"10.3382\/ps\/pev365","article-title":"Interleukin-10 neutralizing antibody for detection of intestinal luminal levels and as a dietary additive in Eimeria challenged broiler chicks","volume":"95","author":"Arendt","year":"2016","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0010","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.vetimm.2007.10.014","article-title":"Coccidia-induced mucogenesis promotes the onset of necrotic enteritis by supporting Clostridium perfringens growth","volume":"122","author":"Collier","year":"2008","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0015","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.vetpar.2009.02.001","article-title":"Host response to simultaneous infections with Eimeria acervulina, maxima and tenella: a cumulation of single responses","volume":"162","author":"Cornelissen","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0020","doi-asserted-by":"crossref","first-page":"5771","DOI":"10.4049\/jimmunol.180.9.5771","article-title":"IL-10: the master regulator of immunity to infection","volume":"180","author":"Couper","year":"2008","journal-title":"J. Immunol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0025","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1177\/104063870701900321","article-title":"Toxinotypes of clostridium perfringens isolated from sick and healthy avian species","volume":"19","author":"Crespo","year":"2007","journal-title":"J. Vet. Diagnostic Invest."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0030","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1084\/jem.20061462","article-title":"Resolution of a chronic viral infection after interleukin-10 receptor blockade","volume":"203","author":"Ejrnaes","year":"2006","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0035","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0378-1135(03)00106-8","article-title":"Molecular typing of isolates of Clostridium perfringens from healthy and diseased poultry","volume":"94","author":"Engstr\u00f6m","year":"2003","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0040","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s13567-016-0336-y","article-title":"The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis","volume":"47","author":"Goossens","year":"2016","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0045","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1046\/j.1365-2958.1997.6161993.x","article-title":"The carboxy-terminal C2-like domain of the \u03b1-toxin from Clostridium perfringens mediates calcium-dependent membrane recognition","volume":"26","author":"Guillouard","year":"1997","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0050","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1080\/03079457.2015.1005573","article-title":"Inflammatory responses to a Clostridium perfringens type A strain and \u03b1-toxin in primary intestinal epithelial cells of chicken embryos","volume":"44","author":"Guo","year":"2015","journal-title":"Avian. Pathol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0055","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.exppara.2012.08.017","article-title":"Enhanced expression of IL-10 in contrast to IL-12B mRNA in poultry with experimental coccidiosis","volume":"132","author":"Haritova","year":"2012","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0060","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.vetimm.2006.07.007","article-title":"Analysis of chicken cytokine and chemokine gene expression following Eimeria acervulina and Eimeria tenella infections","volume":"114","author":"Hong","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0065","doi-asserted-by":"crossref","first-page":"e26","DOI":"10.1371\/journal.ppat.0040026","article-title":"NetB, a new toxin that is associated with avian necrotic enteritis caused by Clostridium perfringens","volume":"4","author":"Keyburn","year":"2008","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0070","doi-asserted-by":"crossref","first-page":"433","DOI":"10.3382\/ps.2007-00383","article-title":"Immune-related gene expression in two B-complex disparate genetically inbred Fayoumi chicken lines following Eimeria maxima infection","volume":"87","author":"Kim","year":"2008","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0075","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.dci.2017.02.008","article-title":"Development and characterization of mouse monoclonal antibodies reactive with chicken CXCLi2","volume":"72","author":"Kim","year":"2017","journal-title":"Dev. Comparat. Immunol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0080","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.vetimm.2011.08.001","article-title":"Development and characterization of mouse monoclonal antibodies reactive with chicken interleukin-2 receptor alpha chain (CD25)","volume":"144","author":"Lee","year":"2011","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0085","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.cimid.2011.01.003","article-title":"Development and characterization of mouse monoclonal antibodies reactive with chicken CD80","volume":"34","author":"Lee","year":"2011","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0090","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1017\/S0007114512006083","article-title":"Dietary supplementation of young broiler chickens with Capsicum and turmeric oleoresins increases resistance to necrotic enteritis","volume":"110","author":"Lee","year":"2013","journal-title":"Br. J. Nutr."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0095","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.rvsc.2017.03.019","article-title":"Dietary Allium hookeri reduces inflammatory response and increases expression of intestinal tight junction proteins in LPS-induced young broiler chicken","volume":"112","author":"Lee","year":"2017","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0100","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.3382\/ps\/pey031","article-title":"Allium hookeri supplementation improves intestinal immune response against necrotic enteritis in young broiler chickens","volume":"97","author":"Lee","year":"2018","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0105","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1637\/11507-093016-Reg.1","article-title":"Characterization of Clostridium perfringens strains isolated from healthy and necrotic enteritis-afflicted broiler Chickens","volume":"61","author":"Li","year":"2017","journal-title":"Avian Dis."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0110","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1016\/j.bjm.2017.06.002","article-title":"Adhesion and invasion of Clostridium perfringens type a into epithelial cells","volume":"48","author":"Llanco","year":"2017","journal-title":"Braz. J. Microbiol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0115","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.3382\/ps.2012-02903","article-title":"Characterization of clostridium perfringens isolates from healthy turkeys and from turkeys with necrotic enteritis","volume":"92","author":"Lyhs","year":"2013","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0120","doi-asserted-by":"crossref","first-page":"6858","DOI":"10.1016\/j.vaccine.2017.10.030","article-title":"Oral vaccination of broiler chickens against necrotic enteritis using a non-virulent NetB positive strain of Clostridium perfringens type A","volume":"35","author":"Mishra","year":"2017","journal-title":"Vaccine"},{"key":"10.1016\/j.vetimm.2018.10.001_bib0125","first-page":"1372","article-title":"Processing of gene expression data generated by quantitative real-time RT-PCR","volume":"32","author":"Muller","year":"2002","journal-title":"Biotechniques"},{"key":"10.1016\/j.vetimm.2018.10.001_bib0130","doi-asserted-by":"crossref","first-page":"557","DOI":"10.3382\/japr.2013-00925","article-title":"Necrotic enteritis: Applications for the poultry industry","volume":"23","author":"Paiva","year":"2014","journal-title":"J. Appl. Poult. Res."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0135","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1637\/7997-041707-Reg","article-title":"Immunopathology and cytokine responses in broiler chickens coinfected with Eimeria maxima and clostridium perfringens with the use of an animal model of necrotic enteritis","volume":"52","author":"Park","year":"2008","journal-title":"Avian Dis."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0140","doi-asserted-by":"crossref","DOI":"10.2307\/1589625","article-title":"The prevention of experimentally induced necrotic enteritis in chickens by avoparcin","volume":"23","author":"Prescott","year":"1979","journal-title":"Avian Dis."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0145","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1071\/MA15039","article-title":"Clostridium perfringens extracellular toxins and enzymes: 20 and counting","volume":"36","author":"Revitt-Mills","year":"2015","journal-title":"Microbiol. Aust."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0150","doi-asserted-by":"crossref","DOI":"10.4049\/jimmunol.173.4.2675","article-title":"Cloning and characterization of chicken IL-10 and its role in the immune response to Eimeria maxima","volume":"173","author":"Rothwell","year":"2004","journal-title":"J. Immunol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0155","first-page":"37","article-title":"Pattern of cytokine (IL-6 and IL-10) level as inflammation and anti-inflammation mediator of multiple organ dysfunction syndrome (MODS) in polytrauma","volume":"6","author":"Sapan","year":"2016","journal-title":"Int. J. Burns Trauma"},{"key":"10.1016\/j.vetimm.2018.10.001_bib0160","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1016\/j.bbamcr.2011.01.034","article-title":"The pro- and anti-inflammatory properties of the cytokine interleukin-6","volume":"1813","author":"Scheller","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.vetimm.2018.10.001_bib0165","first-page":"23","article-title":"Coccidiosis: recent advancements in the immunobiology of Eimeria species, preventive measures, and the importance of vaccination as a control tool against these Apicomplexan parasites","volume":"5","author":"Shivaramaiah","year":"2014","journal-title":"Vet. Med. Res. Reports."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0170","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1128\/CMR.9.2.216","article-title":"Clostridial enteric diseases of domestic animals","volume":"9","author":"Songer","year":"1996","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0175","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/nm.1929","article-title":"Effector T cells control lung inflammation during acute influenza virus infection by producing IL-10","volume":"15","author":"Sun","year":"2009","journal-title":"Nat. Med."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0180","doi-asserted-by":"crossref","first-page":"361","DOI":"10.2217\/fmb.13.168","article-title":"Towards an understanding of the role of Clostridium perfringens toxins in human and animal disease","volume":"9","author":"Uzal","year":"2014","journal-title":"Future Microbiol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0185","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1080\/03079450400013162","article-title":"Clostridium perfringens in poultry: an emerging threat for animal and public health","volume":"33","author":"Van Immerseel","year":"2004","journal-title":"Avian Pathol."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0190","first-page":"16","article-title":"The true cost of necrotic enteritis","volume":"31","author":"Wade","year":"2015","journal-title":"World Poult."},{"key":"10.1016\/j.vetimm.2018.10.001_bib0195","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.dci.2016.04.016","article-title":"Analysis of the function of IL-10 in chickens using specific neutralising antibodies and a sensitive capture ELISA","volume":"63","author":"Wu","year":"2016","journal-title":"Dev. Comp. Immunol."}],"container-title":["Veterinary Immunology and Immunopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165242718303283?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165242718303283?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T07:24:33Z","timestamp":1759044273000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0165242718303283"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10]]},"references-count":39,"alternative-id":["S0165242718303283"],"URL":"https:\/\/doi.org\/10.1016\/j.vetimm.2018.10.001","relation":{},"ISSN":["0165-2427"],"issn-type":[{"value":"0165-2427","type":"print"}],"subject":[],"published":{"date-parts":[[2018,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detection of chicken interleukin-10 production in intestinal epithelial cells and necrotic enteritis induced by Clostridium perfringens using capture ELISA","name":"articletitle","label":"Article Title"},{"value":"Veterinary Immunology and Immunopathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetimm.2018.10.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}