{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T07:15:06Z","timestamp":1782458106467,"version":"3.54.5"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,3,26]],"date-time":"2020-03-26T00:00:00Z","timestamp":1585180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012895","name":"Zoetis.","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Immunology and Immunopathology"],"published-print":{"date-parts":[[2020,5]]},"DOI":"10.1016\/j.vetimm.2020.110034","type":"journal-article","created":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T11:36:59Z","timestamp":1583494619000},"page":"110034","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"special_numbering":"C","title":["T cell epitope content comparison (EpiCC) analysis demonstrates a bivalent PCV2 vaccine has greater T cell epitope overlap with field strains than monovalent PCV2 vaccines"],"prefix":"10.1016","volume":"223","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2349-0818","authenticated-orcid":false,"given":"Meggan","family":"Bandrick","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres H.","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prerak","family":"Desai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6149-9103","authenticated-orcid":false,"given":"Gonzalo","family":"Rincon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William D.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances E.","family":"Terry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne S.","family":"De Groot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis L.","family":"Foss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetimm.2020.110034_bib0005","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1111\/tbed.12721","article-title":"Retrospective study of porcine circovirus type 2 infection reveals a novel genotype PCV2f","volume":"65","author":"Bao","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.vetimm.2020.110034_bib0010","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.vaccine.2010.10.050","volume":"29","author":"Beach","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.vetimm.2020.110034_bib0015","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1093\/molbev\/mst028","article-title":"Hierarchical and spatially explicit clustering of DNA sequences with BAPS software","volume":"30","author":"Cheng","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0020","first-page":"1","article-title":"Antigenic determinants of possible vaccine escape by porcine circovirus subtype 2b viruses","author":"Constans","year":"2015","journal-title":"Bioinform. Biol. Insights"},{"key":"10.1016\/j.vetimm.2020.110034_bib0025","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.virol.2011.10.014","article-title":"Low levels of diversity among genomes of porcine circovirus type 1 (PCV1) points to differential adaptive selection between Porcine circoviruses","volume":"422","author":"Cortey","year":"2012","journal-title":"Virology"},{"key":"10.1016\/j.vetimm.2020.110034_bib0030","doi-asserted-by":"crossref","first-page":"6297","DOI":"10.1128\/JVI.78.12.6297-6303.2004","article-title":"A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs","volume":"78","author":"Fenaux","year":"2004","journal-title":"J. Virol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0035","doi-asserted-by":"crossref","first-page":"12813","DOI":"10.1128\/JVI.01719-09","article-title":"Insights into the evolutionary history of an emerging livestock pathogen: porcine circovirus 2","volume":"83","author":"Firth","year":"2009","journal-title":"J. Virol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0040","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.vaccine.2007.12.019","article-title":"Porcine circovirus type 2 (PCV2) vaccination of conventional pigs prevents viremia against PCV2 isolates of different genotypes and geographic origins","volume":"26","author":"Fort","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.vetimm.2020.110034_bib0045","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0208585","article-title":"Porcine circovirus 2 (PCV-2) genotype update and proposal of a new genotyping methodology","volume":"13","author":"Franzo","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.vetimm.2020.110034_bib0050","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.ympev.2016.04.028","article-title":"Phylodynamic analysis of porcine circovirus type 2 reveals global waves of emerging genotypes and the circulation of recombinant forms","volume":"100","author":"Franzo","year":"2016","journal-title":"Mol. Phylogenet. Evol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0055","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.rvsc.2013.06.004","article-title":"Association of concurrent porcine circovirus (PCV) 2a and 2b infection with PCV associated disease in vaccinated pigs","volume":"95","author":"Gerber","year":"2013","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.vetimm.2020.110034_bib0060","doi-asserted-by":"crossref","DOI":"10.1186\/s12859-015-0724-8","article-title":"Development and validation of an epitope prediction tool for swine (PigMatrix) based on the pocket profile method","volume":"16","author":"Guti\u00e9rrez","year":"2015","journal-title":"BMC Bioinformatics"},{"key":"10.1016\/j.vetimm.2020.110034_bib0065","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0159237","article-title":"In vivo validation of predicted and conserved t cell epitopes in a swine influenza model","volume":"11","author":"Guti\u00e9rrez","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.vetimm.2020.110034_bib0070","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1111\/irv.12513","article-title":"T-cell epitope content comparison (EpiCC) of swine H1 influenza A virus hemagglutinin","volume":"11","author":"Guti\u00e9rrez","year":"2017","journal-title":"Influenza Respir. Viruses"},{"key":"10.1016\/j.vetimm.2020.110034_bib0075","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.virusres.2007.10.013","article-title":"Evidence for recombination between PCV2a and PCV2b in the field","volume":"132","author":"Hesse","year":"2008","journal-title":"Virus Res."},{"issue":"1999\u20134915","key":"10.1016\/j.vetimm.2020.110034_bib0080","doi-asserted-by":"crossref","first-page":"99","DOI":"10.3390\/v9050099","article-title":"Porcine circovirus type 2 (PCV2) vaccines in the context of current molecular epidemiology","volume":"9","author":"Karuppannan","year":"2017","journal-title":"Viruses"},{"key":"10.1016\/j.vetimm.2020.110034_bib0085","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0090","doi-asserted-by":"crossref","DOI":"10.1186\/1297-9716-45-29","article-title":"Pigs naturally exposed to porcine circovirus type 2 (PCV2) generate antibody responses capable to neutralise PCV2 isolates of different genotypes and geographic origins","volume":"45","author":"Kurtz","year":"2014","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetimm.2020.110034_bib0095","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1007\/s00705-009-0379-8","article-title":"Recombination of two porcine circovirus type 2 strains","volume":"154","author":"Lefebvre","year":"2009","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0100","doi-asserted-by":"crossref","first-page":"8135","DOI":"10.1128\/JVI.78.15.8135-8145.2004","article-title":"Epitope mapping of the major capsid protein of type 2 porcine circovirus (PCV2) by using chimeric PCV1 and PCV2","volume":"78","author":"Lekcharoensuk","year":"2004","journal-title":"J. Virol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0105","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1146\/annurev-animal-031412-103720","article-title":"Porcine circovirus type 2 (PCV2): pathogenesis and interaction with the immune system","volume":"1","author":"Meng","year":"2013","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"10.1016\/j.vetimm.2020.110034_bib0110","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.4161\/hv.24615","article-title":"The two-faced T cell epitope: examining the host-microbe interface with JanusMatrix","volume":"9","author":"Moise","year":"2013","journal-title":"Hum. Vaccin. Immunother."},{"key":"10.1016\/j.vetimm.2020.110034_bib0115","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.virol.2006.07.047","article-title":"Molecular evolution of porcine circovirus type 2 genomes: phylogeny and clonality","volume":"357","author":"Olvera","year":"2007","journal-title":"Virology"},{"key":"10.1016\/j.vetimm.2020.110034_bib0120","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.vaccine.2012.11.030","article-title":"A PCV2 vaccine based on genotype 2b is more effective than a 2a-based vaccine to protect against PCV2b or combined PCV2a\/2b viremia in pigs with concurrent PCV2, PRRSV and PPV infection","volume":"31","author":"Opriessnig","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.vetimm.2020.110034_bib0125","doi-asserted-by":"crossref","first-page":"4342","DOI":"10.1016\/j.vaccine.2014.06.004","article-title":"Commercial PCV2a-based vaccines are effective in protecting naturally PCV2b-infected finisher pigs against experimental challenge with a 2012 mutant PCV2","volume":"32","author":"Opriessnig","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vetimm.2020.110034_bib0130","doi-asserted-by":"crossref","first-page":"e01879","DOI":"10.1128\/JVI.01879-16","article-title":"A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure","volume":"91","author":"Palinski","year":"2017","journal-title":"J. Virol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0135","doi-asserted-by":"crossref","first-page":"e9490","DOI":"10.1371\/journal.pone.0009490","article-title":"FastTree 2--approximately maximum-likelihood trees for large alignments","volume":"5","author":"Price","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.vetimm.2020.110034_bib0140","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.vetmic.2015.07.026","article-title":"Genetic variability of porcine circovirus 2 (PCV2) field isolates from vaccinated and non-vaccinated pig herds in Germany","volume":"180","author":"Reiner","year":"2015","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0145","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.vetmic.2011.11.030","article-title":"Antigenic subtyping and epitopes\u2019 competition analysis of porcine circovirus type 2 using monoclonal antibodies","volume":"157","author":"Saha","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0150","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.virusres.2011.10.007","article-title":"Porcine circovirus type 2 (PCV2) infections: clinical signs, pathology and laboratory diagnosis","volume":"164","author":"Segal\u00e9s","year":"2012","journal-title":"Virus Res."},{"key":"10.1016\/j.vetimm.2020.110034_bib0155","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1586\/14760584.2015.983084","article-title":"Best practice and future challenges for vaccination against porcine circovirus type 2","volume":"14","author":"Segal\u00e9s","year":"2015","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.vetimm.2020.110034_bib0160","series-title":"PCV-2 Genotype Definition and Nomenclature, Veterinary Record","first-page":"867","author":"Segal\u00e9s","year":"2008"},{"key":"10.1016\/j.vetimm.2020.110034_bib0165","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.rvsc.2015.10.006","article-title":"Genetic diversity of porcine circovirus type 2 and implications for detection and control","volume":"103","author":"Ssemadaali","year":"2015","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.vetimm.2020.110034_bib0170","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1089\/vim.2006.0106","article-title":"T lymphocyte epitope mapping of porcine circovirus type 2","volume":"20","author":"Stevenson","year":"2007","journal-title":"Viral Immunol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0175","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/9858","article-title":"Generation of tissue-specific and promiscuous HLA ligand databases using DNA microarrays and virtual HLA class II matrices","volume":"17","author":"Sturniolo","year":"1999","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0180","doi-asserted-by":"crossref","first-page":"4079","DOI":"10.1016\/j.vaccine.2012.04.022","article-title":"Antibody responses following vaccination versus infection in a porcine circovirus-type 2 (PCV2) disease model show distinct differences in virus neutralization and epitope recognition","volume":"30","author":"Trible","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vetimm.2020.110034_bib0185","doi-asserted-by":"crossref","first-page":"1830","DOI":"10.1099\/vir.0.000100","article-title":"Global molecular genetic analysis of porcine circovirus type 2 (PCV2) sequences confirms the presence of four main PCV2 genotypes and reveals a rapid increase of PCV2d","volume":"96","author":"Xiao","year":"2015","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0190","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.vetmic.2016.11.009","article-title":"PCV2d-2 is the predominant type of PCV2 DNA in pig samples collected in the U.S. during 2014\u20132016","volume":"197","author":"Xiao","year":"2016","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetimm.2020.110034_bib0195","doi-asserted-by":"crossref","first-page":"e383","DOI":"10.1111\/tbed.12768","article-title":"Phylogenetic and genetic variation analyses of porcine circovirus type 2 isolated from China","volume":"65","author":"Yang","year":"2017","journal-title":"Transbound. Emerg. Dis."}],"container-title":["Veterinary Immunology and Immunopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016524272030060X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016524272030060X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T07:25:26Z","timestamp":1759044326000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016524272030060X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5]]},"references-count":39,"alternative-id":["S016524272030060X"],"URL":"https:\/\/doi.org\/10.1016\/j.vetimm.2020.110034","relation":{},"ISSN":["0165-2427"],"issn-type":[{"value":"0165-2427","type":"print"}],"subject":[],"published":{"date-parts":[[2020,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"T cell epitope content comparison (EpiCC) analysis demonstrates a bivalent PCV2 vaccine has greater T cell epitope overlap with field strains than monovalent PCV2 vaccines","name":"articletitle","label":"Article Title"},{"value":"Veterinary Immunology and Immunopathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetimm.2020.110034","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"110034"}}