{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:02:48Z","timestamp":1759104168603,"version":"3.44.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Immunology and Immunopathology"],"published-print":{"date-parts":[[2020,10]]},"DOI":"10.1016\/j.vetimm.2020.110101","type":"journal-article","created":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T11:56:08Z","timestamp":1596714968000},"page":"110101","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Detection of allergen-specific antibody-secreting cells in dogs by ELISPOT"],"prefix":"10.1016","volume":"228","author":[{"given":"Michael P.","family":"Pelst","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Maina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Vandenabeele","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myriam","family":"Hesta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Cox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetimm.2020.110101_bib0005","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.immuni.2019.01.018","article-title":"The secret life of IgE-producing cells","volume":"50","author":"Aranda","year":"2019","journal-title":"Immunity"},{"key":"10.1016\/j.vetimm.2020.110101_bib0010","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1111\/all.12796","article-title":"Patterns of IgE sensitization in house dust mite-allergic patients: implications for allergen immunotherapy","volume":"71","author":"Batard","year":"2016","journal-title":"Allergy"},{"key":"10.1016\/j.vetimm.2020.110101_bib0015","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.vetimm.2012.01.003","article-title":"Patch testing and allergen-specific serum IgE and IgG antibodies in the diagnosis of canine adverse food reactions","volume":"145","author":"Bethlehem","year":"2012","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"19\u201324","key":"10.1016\/j.vetimm.2020.110101_bib0020","first-page":"e15","article-title":"Serum anti-Staphylococcus pseudintermedius IgE and IgG antibodies in dogs with atopic dermatitis and nonatopic dogs","volume":"24","author":"Bexley","year":"2013","journal-title":"Vet. Dermatol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0025","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1111\/j.1398-9995.2007.01393.x","article-title":"Allergenic pollen and pollen allergy in Europe","volume":"62","author":"D\u2019Amato","year":"2007","journal-title":"Allergy"},{"key":"10.1016\/j.vetimm.2020.110101_bib0030","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1111\/j.1439-0442.2005.00756.x","article-title":"The effect of different isolation procedures on canine leucocyte populations and on lectin\u2010induced lymphocyte proliferation","volume":"52","author":"De Bruin","year":"2005","journal-title":"J. Vet. Med. Ser. A"},{"key":"10.1016\/j.vetimm.2020.110101_bib0035","doi-asserted-by":"crossref","first-page":"103","DOI":"10.21825\/vdt.v88i2.16034","article-title":"Allergie bij de hond: een bevraging van dierenartsen en eigenaars over de toestand in Vlaanderen","volume":"88","author":"Depessemier","year":"2019","journal-title":"Vlaams Diergeneeskundig Tijdschrift"},{"key":"10.1016\/j.vetimm.2020.110101_bib0040","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.immuni.2006.12.006","article-title":"Unique maturation program of the IgE response in vivo","volume":"26","author":"Erazo","year":"2007","journal-title":"Immunity"},{"key":"10.1016\/j.vetimm.2020.110101_bib0045","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/vde.12412","article-title":"Western blot analysis of sera from dogs with suspected food allergy","volume":"28","author":"Favrot","year":"2017","journal-title":"Vet. Dermatol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0050","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0165-2427(03)00033-3","article-title":"Serum IgE and IgG responses to food antigens in normal and atopic dogs, and dogs with gastrointestinal disease","volume":"92","author":"Foster","year":"2003","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0055","doi-asserted-by":"crossref","first-page":"291","DOI":"10.2460\/ajvr.2000.61.291","article-title":"Bovine respiratory syncytial virus-specific IgE is associated with interleukin-2 and -4, and interferon-gamma expression in pulmonary lymph of experimentally infected calves","volume":"61","author":"Gershwin","year":"2000","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.vetimm.2020.110101_bib0060","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/vde.12137","article-title":"Food-specific serum IgE and IgG reactivity in dogs with and without skin disease: lack of correlation between laboratories","volume":"25","author":"Hardy","year":"2014","journal-title":"Vet. Dermatol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0065","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1186\/s12917-015-0515-5","article-title":"Canine atopic dermatitis: detailed guidelines for diagnosis and allergen identification","volume":"11","author":"Hensel","year":"2015","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.vetimm.2020.110101_bib0070","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0165-2427(94)05298-7","article-title":"Concentrations of total serum IgE, IgA, and IgG in atopic and parasitized dogs","volume":"44","author":"Hill","year":"1995","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0075","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1136\/vr.158.16.533","article-title":"Survey of the prevalence, diagnosis and treatment of dermatological conditions in small animals in general practice","volume":"158","author":"Hill","year":"2006","journal-title":"Vet. Rec."},{"key":"10.1016\/j.vetimm.2020.110101_bib0080","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0165-2427(01)00313-0","article-title":"The ACVD task force on canine atopic dermatitis (XVII): intradermal testing","volume":"81","author":"Hillier","year":"2001","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0085","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0165-2427(01)00296-3","article-title":"The ACVD task force on canine atopic dermatitis (I): incidence and prevalence","volume":"81","author":"Hillier","year":"2001","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0090","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/j.1939-1676.2004.tb00131.x","article-title":"Lymphocyte blastogenic responses to inciting food allergens in dogs with food hypersensitivity","volume":"18","author":"Ishida","year":"2004","journal-title":"J. Vet. Intern. Med."},{"key":"10.1016\/j.vetimm.2020.110101_bib0095","doi-asserted-by":"crossref","first-page":"397","DOI":"10.4168\/aair.2013.5.6.397","article-title":"The effects of storage conditions on the stability of house dust mite extracts","volume":"5","author":"Jeong","year":"2013","journal-title":"Allergy Asthma Immunol. Res."},{"key":"10.1016\/j.vetimm.2020.110101_bib0100","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1002\/eji.200322919","article-title":"IL-10 and TGF-beta cooperate in the regulatory T cell response to mucosal allergens in normal immunity and specific immunotherapy","volume":"33","author":"Jutel","year":"2003","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0105","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1002\/eji.200535495","article-title":"Inefficient processing of mRNA for the membrane form of IgE is a genetic mechanism to limit recruitment of IgE-secreting cells","volume":"36","author":"Karnowski","year":"2006","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0110","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0022-1759(97)00030-6","article-title":"Combined immunomagnetic cell sorting and ELISPOT assay for the phenotypic characterization of specific antibody-forming cells","volume":"203","author":"Lakew","year":"1997","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.vetimm.2020.110101_bib0115","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000151243","article-title":"The principle of homologous groups in regulatory affairs of allergen products--a proposal","volume":"148","author":"Lorenz","year":"2009","journal-title":"Int. Arch. Allergy Immunol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0120","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1111\/vde.12536","article-title":"An assessment of a Western blot method for the investigation of canine cutaneous adverse food reactions","volume":"29","author":"Maina","year":"2018","journal-title":"Vet. Dermatol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0125","doi-asserted-by":"crossref","first-page":"194","DOI":"10.2460\/javma.241.2.194","article-title":"Current understanding of the pathophysiologic mechanisms of canine atopic dermatitis","volume":"241","author":"Marsella","year":"2012","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"10.1016\/j.vetimm.2020.110101_bib0130","doi-asserted-by":"crossref","DOI":"10.1155\/2011\/302739","article-title":"Serum and salivary IgE, IgA, and IgG4 antibodies to Dermatophagoides pteronyssinus and its major allergens, Der p1 and Der p2, in allergic and nonallergic children","volume":"2011","author":"Miranda","year":"2011","journal-title":"Clin. Dev. Immunol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0135","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1111\/j.1751-0813.1997.tb11264.x","article-title":"Serum and skin IgA concentrations in normal and atopic dogs","volume":"75","author":"Mueller","year":"1997","journal-title":"Aust. Vet. J."},{"key":"10.1016\/j.vetimm.2020.110101_bib0140","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12917-016-0633-8","article-title":"Critically appraised topic on adverse food reactions of companion animals (2): common food allergen sources in dogs and cats","volume":"12","author":"Mueller","year":"2016","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.vetimm.2020.110101_bib0145","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/s13567-015-0210-3","article-title":"Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae","volume":"46","author":"Nguyen","year":"2015","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetimm.2020.110101_bib0150","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/s12917-017-0973-z","article-title":"Critically appraised topic on adverse food reactions of companion animals (3): prevalence of cutaneous adverse food reactions in dogs and cats","volume":"13","author":"Olivry","year":"2017","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.vetimm.2020.110101_bib0155","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1111\/j.1365-3164.2010.00889.x","article-title":"Treatment of canine atopic dermatitis: 2010 clinical practice guidelines from the International Task Force on Canine Atopic Dermatitis","volume":"21","author":"Olivry","year":"2010","journal-title":"Vet. Dermatol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0160","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/vde.12201","article-title":"Review: the role of antibodies, autoantigens and food allergens in canine atopic dermatitis","volume":"26","author":"Pucheu-Haston","year":"2015","journal-title":"Vet. Dermatol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0165","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1136\/vr.100710","article-title":"Characterisation of dog sensitisation to grass pollen in western France from 1999 to 2010","volume":"172","author":"Roussel","year":"2013","journal-title":"Vet. Rec."},{"key":"10.1016\/j.vetimm.2020.110101_bib0170","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1038\/nprot.2013.058","article-title":"Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood","volume":"8","author":"Saletti","year":"2013","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.vetimm.2020.110101_bib0175","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0165-2427(99)00040-9","article-title":"Canine atopic dermatitis in Greece: clinical observations and the prevalence of positive intradermal test reactions in 91 spontaneous cases","volume":"69","author":"Saridomichelakis","year":"1999","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0180","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1365-3164.2008.00654.x","article-title":"Assessment of cross-reactivity among five species of house dust and storage mites","volume":"19","author":"Saridomichelakis","year":"2008","journal-title":"Vet. Dermatol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0185","doi-asserted-by":"crossref","first-page":"S73","DOI":"10.1016\/j.jaci.2009.11.017","article-title":"IgE, mast cells, basophils, and eosinophils","volume":"125","author":"Stone","year":"2010","journal-title":"J. Allergy Clin. Immunol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0190","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0165-2427(94)05306-D","article-title":"Canine atopic disease: the prevalence of positive intradermal skin tests at two sites in the north and south of Great Britain","volume":"44","author":"Sture","year":"1995","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetimm.2020.110101_bib0195","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.tvjl.2018.12.014","article-title":"Testing for food-specific antibodies in saliva and blood of food allergic and healthy dogs","volume":"245","author":"Udraite Vovk","year":"2019","journal-title":"Vet. J."},{"key":"10.1016\/j.vetimm.2020.110101_bib0200","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1136\/bmj.325.7361.411","article-title":"Sensitisation to airborne moulds and severity of asthma: cross sectional study from European Community respiratory health survey","volume":"325","author":"Zureik","year":"2002","journal-title":"BMJ"}],"container-title":["Veterinary Immunology and Immunopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165242720301276?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165242720301276?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T07:26:13Z","timestamp":1759044373000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0165242720301276"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":40,"alternative-id":["S0165242720301276"],"URL":"https:\/\/doi.org\/10.1016\/j.vetimm.2020.110101","relation":{},"ISSN":["0165-2427"],"issn-type":[{"type":"print","value":"0165-2427"}],"subject":[],"published":{"date-parts":[[2020,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detection of allergen-specific antibody-secreting cells in dogs by ELISPOT","name":"articletitle","label":"Article Title"},{"value":"Veterinary Immunology and Immunopathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetimm.2020.110101","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"110101"}}