{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:15:26Z","timestamp":1790727326667,"version":"4.1.0"},"reference-count":29,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2005,8,1]],"date-time":"2005-08-01T00:00:00Z","timestamp":1122854400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2005,8]]},"DOI":"10.1016\/j.vetmic.2005.05.002","type":"journal-article","created":{"date-parts":[[2005,6,10]],"date-time":"2005-06-10T15:55:01Z","timestamp":1118418901000},"page":"89-94","source":"Crossref","is-referenced-by-count":45,"title":["Evaluation of the recombinant LipL32 in enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis"],"prefix":"10.1016","volume":"109","author":[{"given":"Maria Rosa Quaresma","family":"Bomfim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Ko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matilde Cota","family":"Koury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2005.05.002_bib1","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/0378-1135(82)90050-5","article-title":"Bovine IgM and IgG response to Leptospira interrogans serovar hardjo as measured by enzyme-immunoassay","volume":"7","author":"Adler","year":"1982","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2005.05.002_bib2","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/0378-1135(90)90036-U","article-title":"Evaluation of an ELISA for the diagnosis of experimentally induced and naturally occurring Leptospira hardjo infections in cattle","volume":"21","author":"Bercovich","year":"1990","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2005.05.002_bib3","first-page":"285","article-title":"Diagnostic specificity, sensitivity and cross-reactivity of an enzyme-linked immunosorbent assay for the detection of antibody against L. interrogans serovars pomona, sejroe and hardjo in cattle","volume":"53","author":"Cho","year":"1989","journal-title":"Can. J. Vet. Res."},{"key":"10.1016\/j.vetmic.2005.05.002_bib4","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/0378-1135(85)90026-4","article-title":"The use of enzyme-linked immunosorbent assay (ELISA) to detect the IgM and IgG antibody response to Leptospira interrogans serovar hardjo, pomona and tarassovi in cattle","volume":"10","author":"Cousins","year":"1985","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2005.05.002_bib5","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.vetmic.2004.07.018","article-title":"Recombinant LipL32 antigen-based single serum dilution ELISA for detection of canine leptospirosis","volume":"103","author":"Dey","year":"2004","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2005.05.002_bib6","first-page":"39","article-title":"Nutrition of Leptospira pomona and growth of 13 other serotypes: a serum-free medium employing oleic albumin complex","volume":"26","author":"Ellinghausen","year":"1965","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.vetmic.2005.05.002_bib7","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/0167-5877(84)90085-0","article-title":"Bovine leptospirosis in tropics: prevalence, pathogenesis and control","volume":"2","author":"Ellis","year":"1984","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.vetmic.2005.05.002_bib8","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0749-0720(15)30532-6","article-title":"Leptospirosis as a cause of reproductive failure","volume":"10","author":"Ellis","year":"1994","journal-title":"Vet. Clin. North Am. Food Anim. Pract."},{"key":"10.1016\/j.vetmic.2005.05.002_bib9","volume":"67","author":"Faine","year":"1982"},{"key":"10.1016\/j.vetmic.2005.05.002_bib10","first-page":"1422","article-title":"Antibody response to genus and serovar-specific leptospiral antigens in leptospira-infected cows","volume":"46","author":"Fairbrother","year":"1985","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.vetmic.2005.05.002_bib11","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.1128\/JCM.39.9.3303-3310.2001","article-title":"Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of Leptospirosis","volume":"39","author":"Flannery","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetmic.2005.05.002_bib12","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0378-1135(91)90055-K","article-title":"The serological response of calves to Leptospira interrogans serovar hardjo vaccines and infection as measured by the microscopic agglutination test and anti-IgM and anti-IgG enzyme-linked immunosorbent assay","volume":"26","author":"Goddard","year":"1991","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2005.05.002_bib13","doi-asserted-by":"crossref","first-page":"2276","DOI":"10.1128\/IAI.68.4.2276-2285.2000","article-title":"The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection","volume":"68","author":"Haake","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetmic.2005.05.002_bib14","doi-asserted-by":"crossref","first-page":"2818","DOI":"10.1128\/JB.186.9.2818-2828.2004","article-title":"Molecular evolution and mosaicism of leptospiral outer membrane proteins involves horizontal DNA transfer","volume":"186","author":"Haake","year":"2004","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.vetmic.2005.05.002_bib15","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1128\/JCM.36.1.11-14.1998","article-title":"Evaluation of the indirect hemagglutination assay for diagnosis of acute leptospirosis","volume":"36","author":"Levett","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetmic.2005.05.002_bib16","unstructured":"National Research Council (1996). Guide for the Care and Use of Laboratory Animals. National Academy Press, Washington, DC."},{"key":"10.1016\/j.vetmic.2005.05.002_bib17","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0378-1135(99)00133-9","article-title":"A rapid latex agglutination test for detection of leptospiral antibodies","volume":"70","author":"Ramadass","year":"1999","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2005.05.002_bib18","first-page":"260","article-title":"Development and initial evaluation of an indirect enzyme-linked immunosorbent assay for detection of Leptospira interrogans serovar hardjo antibodies in bovine sera","volume":"61","author":"Surujballi","year":"1997","journal-title":"Can. J. Vet. Res."},{"key":"10.1016\/j.vetmic.2005.05.002_bib19","first-page":"267","article-title":"Development of a monoclonal antibody-based competitive enzyme-linked immunosorbent assay for detection of Leptospira borgpetersenii serovar hardjo type hardjobovis antibodies in bovine sera","volume":"61","author":"Surujballi","year":"1997","journal-title":"Can. J. Vet. Res."},{"key":"10.1016\/j.vetmic.2005.05.002_bib20","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1128\/CDLI.8.1.40-43.2001","article-title":"Competitive enzyme-linked immunosorbent assay for detection of Leptospira interrogans serovar pomona antibodies in bovine sera","volume":"8","author":"Surujballi","year":"2001","journal-title":"Clin. Diagn. Lab. Immun."},{"key":"10.1016\/j.vetmic.2005.05.002_bib21","first-page":"1","article-title":"An indirect enzyme-linked immunosorbent assay for the detection of bovine antibodies to multiple Leptospira serovars","volume":"68","author":"Surujballi","year":"2004","journal-title":"Can. J. Vet. Res."},{"key":"10.1016\/j.vetmic.2005.05.002_bib22","first-page":"2244","article-title":"Bovine leptospirosis: bacteriologic versus serologic diagnosis of cows at slaughter","volume":"44","author":"Thiermann","year":"1983","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.vetmic.2005.05.002_bib23","first-page":"97","article-title":"Detection of the enzyme-linked immunosorbent assay (ELISA) as new method for serological diagnosis of bovine leptospirosis","volume":"3","author":"Thiermann","year":"1983","journal-title":"Int. Symp. Vet. Lab. Diagn."},{"key":"10.1016\/j.vetmic.2005.05.002_bib24","first-page":"884","article-title":"Enzyme-linked immunosorbent assay for the detection of antibodies to L. interrogans serovar hardjo and pomona in cattle","volume":"44","author":"Thiermann","year":"1983","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.vetmic.2005.05.002_bib25","first-page":"758","article-title":"Isolation of leptospires in diagnosis of leptospirosis","author":"Thiermann","year":"1984","journal-title":"Modern Vet. Pract."},{"key":"10.1016\/j.vetmic.2005.05.002_bib26","first-page":"722","article-title":"Leptospirosis: current developments and trends","volume":"184","author":"Thiermann","year":"1984","journal-title":"Am. Vet. Med. Assoc."},{"key":"10.1016\/j.vetmic.2005.05.002_bib27","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1177\/104063879000200413","article-title":"Evaluation of an enzyme immunoassay for diagnosis of bovine leptospirosis caused by L. interrogans serovar hardjo type hardjobovis","volume":"2","author":"Trueba","year":"1990","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.vetmic.2005.05.002_bib28","unstructured":"Vasconcellos, S.A., Barbarini, J.O., Umehara, O., Morais, Z.M., Cortez, A., Pinheiro, S.R., Ferreira, F., F\u00e1vero, A.C.M., Neto, J.S.F., 1997. Leptospirose bovina. N\u00edveis de ocorr\u00eancia e sorotipos predominantes em rebanhos dos estados de Minas Gerais, S\u00e3o Paulo, Rio de Janeiro, Paran\u00e1, Rio Grande do Sul e Mato Grosso do Sul. Per\u00edodo de janeiro a abril de 1996. Arq. Inst. Biol. S\u00e3o Paulo 64, 7\u201317."},{"key":"10.1016\/j.vetmic.2005.05.002_bib29","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0378-1135(99)00103-0","article-title":"Development of an ELISA to detect antibodies to a protective lipopolysaccharide fraction of Leptospira borgpetersenii serovar hardjo in catlle","volume":"69","author":"Yan","year":"1999","journal-title":"Vet. Microbiol."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113505001562?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113505001562?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T16:03:54Z","timestamp":1586275434000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113505001562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,8]]},"references-count":29,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2005,8]]}},"alternative-id":["S0378113505001562"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2005.05.002","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2005,8]]}}}