{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T23:01:32Z","timestamp":1792364492299,"version":"4.4.0"},"reference-count":38,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2007,3]]},"DOI":"10.1016\/j.vetmic.2006.11.008","type":"journal-article","created":{"date-parts":[[2006,11,22]],"date-time":"2006-11-22T15:22:48Z","timestamp":1164208968000},"page":"29-38","source":"Crossref","is-referenced-by-count":68,"title":["Cloning and expression of Rift Valley fever virus nucleocapsid (N) protein and evaluation of a N-protein based indirect ELISA for the detection of specific IgG and IgM antibodies in domestic ruminants"],"prefix":"10.1016","volume":"121","author":[{"given":"Jos\u00e9 Manuel","family":"Fafetine","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"Tijhaar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janusz T.","family":"Paweska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds C.B.G.","family":"Neves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Hendriks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Swanepoel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.A.W.","family":"Coetzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herman F.","family":"Egberink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor P.M.G.","family":"Rutten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2006.11.008_bib1","first-page":"247","article-title":"Protein G ELISA for detection of antibodies against Toxoplasma SAG1 in dromedaries","volume":"32","author":"Abdu-Zeid","year":"2002","journal-title":"J. Egypt Soc. Parasitol."},{"key":"10.1016\/j.vetmic.2006.11.008_bib2","first-page":"155","article-title":"Rift Valley fever vaccine\u2014antibody and immune response in cattle to a live and an inactivated vaccine","volume":"50","author":"Barnard","year":"1979","journal-title":"J. S. Afr. Vet. Assoc."},{"key":"10.1016\/j.vetmic.2006.11.008_bib3","series-title":"OIE Manual of Standards for Diagnostic Tests and Vaccines","first-page":"144","article-title":"Rift Valley Fever","author":"Barnard","year":"2000"},{"issue":"4","key":"10.1016\/j.vetmic.2006.11.008_bib4","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1007\/BF02013104","article-title":"Streptococcal protein G: a sensitive tool for detection of antibodies to human immunodeficiency virus protein in Western blot analysis","volume":"6","author":"Bjorck","year":"1987","journal-title":"Eur. J. Clin. Microbiol."},{"issue":"6","key":"10.1016\/j.vetmic.2006.11.008_bib5","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.1128\/JCM.37.6.2010-2012.1999","article-title":"Detection of Toscana virus-specific immmunoglobulins G and M by enzyme-linked immunosorbent assay based on recombinant viral nucleoprotein","volume":"37","author":"Ciufolini","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetmic.2006.11.008_bib6","series-title":"Methods in Molecular Biology","article-title":"ELISA theory and practice","author":"Crowther","year":"1995"},{"issue":"3","key":"10.1016\/j.vetmic.2006.11.008_bib7","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1016\/j.bbrc.2004.07.189","article-title":"The solubility and stability of recombinant proteins are increased by their fusion to NusA","volume":"322","author":"De Marco","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"5","key":"10.1016\/j.vetmic.2006.11.008_bib8","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1128\/JCM.35.5.1122-1130.1997","article-title":"Serological diagnosis of Hantavirus infections by an enzyme-linked immunosorbent assay based on detection of immunoglobulin G and M responses to recombinant nucleocapsid proteins of five viral serotypes","volume":"35","author":"Elgh","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetmic.2006.11.008_bib9","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1128\/JCM.10.4.583-585.1979","article-title":"Detection of antibodies to alphavirus by enzyme-linked immunosorbent assay","volume":"10","author":"Forghani","year":"1979","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetmic.2006.11.008_bib10","doi-asserted-by":"crossref","first-page":"4456","DOI":"10.1128\/JCM.39.12.4456-4461.2001","article-title":"Quantitative real-time PCR detection of Rift Valley fever virus and its application to evaluation of antiviral compounds","volume":"39","author":"Garcia","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetmic.2006.11.008_bib11","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0022-1759(95)00078-O","article-title":"Two-graph receiver operating characteristics (TG-ROC)\u2014a Microsoft-Excel template for the selection of cut-off values in diagnostic tests","volume":"185","author":"Greiner","year":"1995","journal-title":"J. Immunol. Meth."},{"key":"10.1016\/j.vetmic.2006.11.008_bib12","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0022-1759(95)00121-P","article-title":"A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests","volume":"185","author":"Greiner","year":"1995","journal-title":"J. Immunol. Meth."},{"key":"10.1016\/j.vetmic.2006.11.008_bib13","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0167-5877(00)00116-1","article-title":"Application of diagnostic tests in veterinary epidemiological studies","volume":"45","author":"Greiner","year":"2000","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.vetmic.2006.11.008_bib14","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1006\/mcpr.1996.0075","article-title":"Detection of Rift Valley fever virus in mosquitoes by RT-PCR","volume":"11","author":"Ibrahim","year":"1997","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.vetmic.2006.11.008_bib15","doi-asserted-by":"crossref","first-page":"469","DOI":"10.20506\/rst.17.2.1119","article-title":"Validation of serological assays for diagnosis of infectious diseases","volume":"17","author":"Jacobson","year":"1998","journal-title":"Rev. Sci. Tech. Off. Int. Epiz."},{"issue":"3","key":"10.1016\/j.vetmic.2006.11.008_bib16","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1128\/JCM.31.3.677-680.1993","article-title":"Puumala virus antibody and immunoglobulin G avidity assays based on a recombinant nucleocapsid antigen","volume":"31","author":"Kallio-Kokko","year":"1993","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetmic.2006.11.008_bib17","doi-asserted-by":"crossref","first-page":"547","DOI":"10.4269\/ajtmh.1934.s1-14.547","article-title":"Laboratory infections with the virus of Rift Valley fever","volume":"14","author":"Kitchen","year":"1934","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.vetmic.2006.11.008_bib18","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jviromet.2004.12.002","article-title":"Preparation of recombinant African horse sickness virus VP7 antigen via a simple method and validation of a VP7-based indirect ELISA for the detection of group-specific IgG antibodies in horse sera","volume":"125","author":"Maree","year":"2005","journal-title":"J. Virol. Meth."},{"key":"10.1016\/j.vetmic.2006.11.008_bib19","doi-asserted-by":"crossref","first-page":"2157","DOI":"10.1099\/0022-1317-75-9-2157","article-title":"Detection of human antibodies to Crimean-Congo haemorrhagic fever virus using expressed viral nucleocapsid protein","volume":"75","author":"Marriott","year":"1994","journal-title":"J. Gen. Virol."},{"issue":"7","key":"10.1016\/j.vetmic.2006.11.008_bib20","first-page":"1138","article-title":"Enzyme-linked immunosorbent assay for detection of antibodies to Rift Valley fever virus in ovine and bovine sera","volume":"48","author":"Meegan","year":"1987","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.vetmic.2006.11.008_bib21","first-page":"227","article-title":"The use of sucrose\u2013acetone-extracted Rift Valley fever virus antigen derived from cell culture in an indirect enzyme-linked immunosorbent assay and haemagglutination-inhibition test","volume":"62","author":"Paweska","year":"1995","journal-title":"Onderstepoort J. Vet. Res."},{"key":"10.1016\/j.vetmic.2006.11.008_bib22","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0166-0934(03)00228-3","article-title":"IgG-sandwich and IgM capture enzyme-linked immunosorbent assay for the detection of antibodies to Rift Valley fever virus in domestic ruminants","volume":"113","author":"Paweska","year":"2003","journal-title":"J. Virol. Meth."},{"key":"10.1016\/j.vetmic.2006.11.008_bib23","first-page":"49","article-title":"Indirect enzyme-linked immunosorbent assay for the detection of antibody against Rift Valley fever virus in domestic and wild ruminant sera","volume":"70","author":"Paweska","year":"2003","journal-title":"Onderstepoort. J. Vet. Res."},{"issue":"1\/2","key":"10.1016\/j.vetmic.2006.11.008_bib24","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.vetmic.2004.02.003","article-title":"Evaluation of three serum i-ELISA using monoclonal antibodies and protein G as peroxidase conjugate for the diagnosis of bovine brucellosis","volume":"100","author":"Saegerman","year":"2004","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2006.11.008_bib25","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.4269\/ajtmh.1980.29.1359","article-title":"Laboratory safety for arboviruses and certain other viruses of vertebrates","volume":"29","author":"Scherer","year":"1980","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.vetmic.2006.11.008_bib26","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/(SICI)1096-9071(199606)49:2<83::AID-JMV2>3.0.CO;2-F","article-title":"Immunoblot detection of antibodies to Toscana virus","volume":"49","author":"Schwarz","year":"1996","journal-title":"J. Med. Virol."},{"issue":"4","key":"10.1016\/j.vetmic.2006.11.008_bib27","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1128\/JCM.24.4.612-614.1986","article-title":"Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta","volume":"24","author":"Scott","year":"1986","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetmic.2006.11.008_bib28","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.3201\/eid0812.020195","article-title":"Genetic analysis of viruses associated with emergence of Rift Valley fever in Saudi Arabia and Yemen, 2000\u20132001","volume":"12","author":"Shoemaker","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.vetmic.2006.11.008_bib29","doi-asserted-by":"crossref","first-page":"213","DOI":"10.4049\/jimmunol.62.2.213","article-title":"Rift Valley fever accidental infections among laboratory workers","volume":"62","author":"Smithburn","year":"1949","journal-title":"J. Immunol."},{"key":"10.1016\/j.vetmic.2006.11.008_bib30","first-page":"42","article-title":"Studies on the epidemiology of Rift Valley fever","volume":"3","author":"Swanepoel","year":"1976","journal-title":"J. S. Afr. Vet. Ass."},{"key":"10.1016\/j.vetmic.2006.11.008_bib31","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1017\/S0022172400065414","article-title":"Comparison of techniques for demonstrating antibodies to Rift Valley fever virus","volume":"97","author":"Swanepoel","year":"1986","journal-title":"J. Hyg."},{"key":"10.1016\/j.vetmic.2006.11.008_bib32","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1017\/S0022172400065426","article-title":"Comparative pathogenicity and antigenic cross-reactivity of Rift Valley fever and other African phleboviruses in sheep","volume":"97","author":"Swanepoel","year":"1986","journal-title":"J. Hyg."},{"key":"10.1016\/j.vetmic.2006.11.008_bib33","first-page":"688","article-title":"Rift Valley fever","volume":"vol. 1","author":"Swanepoel","year":"1994"},{"key":"10.1016\/j.vetmic.2006.11.008_bib34","first-page":"94","article-title":"Vector competence of a Houston, Texas strain of Aedes albopictus for Rift Valley fever virus","volume":"4","author":"Turrel","year":"1998","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"10.1016\/j.vetmic.2006.11.008_bib35","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/jmv.1890460402","article-title":"Human B-cell epitopes of Puumala virus nucleocapsid protein, the major antigen in early serological response","volume":"46","author":"Vapalahti","year":"1995","journal-title":"J. Med. Virol."},{"issue":"2","key":"10.1016\/j.vetmic.2006.11.008_bib36","doi-asserted-by":"crossref","first-page":"138","DOI":"10.3201\/eid0802.010023","article-title":"An outbreak of Rift Valley fever in Northeastern Kenya, 1997\u20131998","volume":"8","author":"Woods","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.vetmic.2006.11.008_bib37","doi-asserted-by":"crossref","first-page":"435","DOI":"10.20506\/rst.12.2.691","article-title":"Standardisation and validation of enzyme-linked immunosorbent assay techniques for the detection of antibody in infectious disease diagnosis","volume":"12","author":"Wright","year":"1993","journal-title":"Rev. Sci. Tech. Off. Int. Epiz."},{"key":"10.1016\/j.vetmic.2006.11.008_bib38","doi-asserted-by":"crossref","first-page":"824","DOI":"10.20506\/rst.16.3.1061","article-title":"International reference standards: antibody standards for the indirect enzyme-linked immunosorbent assay","volume":"16","author":"Wright","year":"1997","journal-title":"Rev. Sci. Tech. Off. Int. Epiz."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113506004639?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113506004639?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T19:47:05Z","timestamp":1683661625000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113506004639"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":38,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["S0378113506004639"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2006.11.008","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2007,3]]}}}