{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:47:56Z","timestamp":1788871676197,"version":"build-2803163510"},"reference-count":30,"publisher":"Elsevier BV","issue":"3-4","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"University of Wyoming, Agricultural Experiment Station","award":["WYO-395-04"],"award-info":[{"award-number":["WYO-395-04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2010,5]]},"DOI":"10.1016\/j.vetmic.2009.10.010","type":"journal-article","created":{"date-parts":[[2009,10,24]],"date-time":"2009-10-24T04:34:32Z","timestamp":1256358872000},"page":"367-372","source":"Crossref","is-referenced-by-count":15,"title":["Identification of Brucella abortus genes in elk (Cervus elaphus) using in vivo-induced antigen technology (IVIAT) reveals novel markers of infection"],"prefix":"10.1016","volume":"142","author":[{"given":"J.E.","family":"Lowry","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Goodridge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Vernati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.M.","family":"Fluegel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.H.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.P.","family":"Andrews","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2009.10.010_bib1","first-page":"387","article-title":"The detection of Brucella spp. using PCR-ELISA and real-time PCR assays","volume":"50","author":"Al Dahouk","year":"2004","journal-title":"Clin. Lab."},{"key":"10.1016\/j.vetmic.2009.10.010_bib2","doi-asserted-by":"crossref","unstructured":"Anderson, E.S., Paulley, J.T., Gaines, J.M., Valderas, M.W., Martin, D.W., Menscher, E., Brown, T.D., Burns, C.S. Roop II, R.M., 2009. The manganese transporter MntH is a critical virulence determinant for Brucella abortus 2308 in experimentally infected mice. Infect. Immun. (Epup ahead of print on 1 June 2009).","DOI":"10.1128\/IAI.00444-09"},{"issue":"5","key":"10.1016\/j.vetmic.2009.10.010_bib3","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1128\/CVI.13.5.541-546.2006","article-title":"Rapid, sensitive, and specific lateral-flow immunochromatographic device to measure anti-anthrax protective antigen immunoglobulin g in serum and whole blood","volume":"13","author":"Biagini","year":"2006","journal-title":"Clin. Vac. Immunol."},{"issue":"8","key":"10.1016\/j.vetmic.2009.10.010_bib4","doi-asserted-by":"crossref","first-page":"4050","DOI":"10.1128\/IAI.00486-07","article-title":"Role of the Omp25\/Omp31 family in outer membrane properties and virulence of Brucella ovis","volume":"75","author":"Caro-Hernandez","year":"2007","journal-title":"Infect. Immun."},{"issue":"7","key":"10.1016\/j.vetmic.2009.10.010_bib5","doi-asserted-by":"crossref","first-page":"4272","DOI":"10.1128\/IAI.73.7.4272-4280.2005","article-title":"Identification of a novel adhesion molecule involved in the virulence of Legionella pneumophila","volume":"73","author":"Chang","year":"2005","journal-title":"Infect. Immun."},{"issue":"8","key":"10.1016\/j.vetmic.2009.10.010_bib6","doi-asserted-by":"crossref","first-page":"4000","DOI":"10.1128\/IAI.66.8.4000-4003.1998","article-title":"Identification and characterization of a 14-kilodalton Brucella abortus protein reactive with antibodies from naturally and experimentally infected hosts and T lymphocytes from experimentally infected BALB\/c mice","volume":"66","author":"Chirhart-Gilleland","year":"1998","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetmic.2009.10.010_bib7","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1046\/j.1462-5822.2001.00102.x","article-title":"Essential role of the VirB machinery in the maturation of the Brucella abortus containing vacuole","volume":"3","author":"Comerci","year":"2001","journal-title":"Cell Microbiol."},{"key":"10.1016\/j.vetmic.2009.10.010_bib8","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1054\/tube.2002.0337","article-title":"Selective identification of new therapeutic targets of Mycobacterium tuberculosis by IVIAT approach","volume":"82","author":"Deb","year":"2002","journal-title":"Tuberculosis"},{"key":"10.1016\/j.vetmic.2009.10.010_bib9","doi-asserted-by":"crossref","first-page":"110","DOI":"10.7589\/0090-3558-37.1.110","article-title":"Evaluation of the fluorescence polarization assay and comparison to other serological assays for detection of brucellosis in cervids","volume":"37","author":"Gall","year":"2001","journal-title":"J. Wildl. Dis."},{"issue":"5","key":"10.1016\/j.vetmic.2009.10.010_bib10","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1093\/bioinformatics\/bti057","article-title":"PSORTb v.2.0: expanded prediction of bacterial protein subcellular localization and insights gained from comparative proteome analysis","volume":"21","author":"Gardy","year":"2005","journal-title":"Bioinformatics"},{"key":"10.1016\/j.vetmic.2009.10.010_bib11","doi-asserted-by":"crossref","first-page":"277","DOI":"10.20506\/rst.21.2.1333","article-title":"Brucellosis in wildlife","volume":"21","author":"Godfroid","year":"2002","journal-title":"Rev. Sci. Technol. Off. Int. Epiz."},{"key":"10.1016\/j.vetmic.2009.10.010_bib12","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/S0966-842X(00)01775-3","article-title":"IVIAT: a novel method to identify microbial genes expressed specifically during human infections","volume":"8","author":"Handfield","year":"2000","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.vetmic.2009.10.010_bib13","doi-asserted-by":"crossref","first-page":"4102","DOI":"10.1128\/IAI.68.7.4102-4107.2000","article-title":"Identification of genes required for chronic persistence of Brucella abortus in mice","volume":"68","author":"Hong","year":"2000","journal-title":"Infect. Immun."},{"issue":"1","key":"10.1016\/j.vetmic.2009.10.010_bib14","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1128\/CMR.16.1.65-78.2003","article-title":"Molecular host\u2013pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans","volume":"16","author":"Ko","year":"2003","journal-title":"Clin. Microbiol. Rev."},{"issue":"7","key":"10.1016\/j.vetmic.2009.10.010_bib15","doi-asserted-by":"crossref","first-page":"2102","DOI":"10.1128\/jb.178.7.2102-2107.1996","article-title":"An O-antigen processing function for WzX (RfbX) a promising candidate for O-unit flippase","volume":"178","author":"Liu","year":"1996","journal-title":"J. Bacteriol."},{"issue":"1","key":"10.1016\/j.vetmic.2009.10.010_bib16","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1006\/mpat.1998.0206","article-title":"Omp85 proteins of Neisseria gonorrhoeae and Neisseria meningitidis are similar to Haemophilus influenzae D-15-Ag and Pasteurella multocida Oma87","volume":"25","author":"Manning","year":"1998","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.vetmic.2009.10.010_bib17","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1128\/IAI.72.3.1666-1676.2004","article-title":"Glycolytic and gluconeogenic growth of Escherichia coli O157:H7 (EDL933) and E. coli K-12 (MG1655) in the mouse intestine","volume":"72","author":"Miranda","year":"2004","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetmic.2009.10.010_bib18","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1586\/14737159.4.1.115","article-title":"Diagnosis of human brucellosis using PCR","volume":"4","author":"Navarro","year":"2004","journal-title":"Exp. Rev. Mol. Diag."},{"issue":"11","key":"10.1016\/j.vetmic.2009.10.010_bib19","doi-asserted-by":"crossref","first-page":"e377","DOI":"10.1371\/journal.pbio.0040377","article-title":"Assembly factor Omp85 recognizes its outer membrane protein substrates by a species-specific C-Terminal motif","volume":"4","author":"Robert","year":"2006","journal-title":"PLoS Biol."},{"key":"10.1016\/j.vetmic.2009.10.010_bib20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1462-5822.2004.00477.x","article-title":"In vivo induced antigen technology (IVIAT)","volume":"7","author":"Rollins","year":"2005","journal-title":"Cell. Microbiol."},{"issue":"3","key":"10.1016\/j.vetmic.2009.10.010_bib21","doi-asserted-by":"crossref","first-page":"e1824","DOI":"10.1371\/journal.pone.0001824","article-title":"Application of in vivo induced antigen technology (IVIAT) to Bacillus anthracis","volume":"3","author":"Rollins","year":"2008","journal-title":"PLoS One"},{"issue":"7","key":"10.1016\/j.vetmic.2009.10.010_bib22","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1111\/j.1462-5822.2007.00922.x","article-title":"Brucella requires a functional Type IV secretion system to elicit innate immune responses in mice","volume":"9","author":"Roux","year":"2007","journal-title":"Cell Microbiol."},{"issue":"1\u20132","key":"10.1016\/j.vetmic.2009.10.010_bib23","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.vetmic.2004.02.003","article-title":"Evaluation of three i-ELISAs using monoclonal antibodies and protein G as peroxidase conjugate for the diagnosis of bovine brucellosis","volume":"100","author":"Saegerman","year":"2004","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2009.10.010_bib24","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/0378-1135(91)90091-S","article-title":"Biological properties of RB51: a stable rough strain of Brucella abortus","volume":"28","author":"Schurig","year":"1991","journal-title":"Vet. Microbiol."},{"issue":"17","key":"10.1016\/j.vetmic.2009.10.010_bib25","doi-asserted-by":"crossref","first-page":"6231","DOI":"10.1128\/JB.187.17.6231-6237.2005","article-title":"Xanthomonas campestris pv. campestris possesses a single gluconeogenic pathway that is required for virulence","volume":"187","author":"Tang","year":"2005","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.vetmic.2009.10.010_bib26","unstructured":"United States Department of Agriculture (USDA), 2003. Brucellosis in Cervidae:Uniform Methods and Rules. September 30, 2003."},{"key":"10.1016\/j.vetmic.2009.10.010_bib27","doi-asserted-by":"crossref","first-page":"316","DOI":"10.7589\/0090-3558-39.2.316","article-title":"Validation of a Brucella Abortus competitive enzyme-linked immunosorbent assay for use in Rocky Mountain Elk (Cervus Elaphus Nelson)","volume":"39","author":"Van Houten","year":"2003","journal-title":"J. Wildl. Dis."},{"issue":"7","key":"10.1016\/j.vetmic.2009.10.010_bib28","doi-asserted-by":"crossref","first-page":"3927","DOI":"10.1128\/IAI.68.7.3927-3932.2000","article-title":"Complementation of Brucella abortus RB51 with a functional wboA gene results in O-antigen synthesis and enhanced vaccine efficacy but no change in rough phenotype and attenuation","volume":"68","author":"Vemulapalli","year":"2000","journal-title":"Infect. Immun."},{"issue":"10","key":"10.1016\/j.vetmic.2009.10.010_bib29","doi-asserted-by":"crossref","first-page":"3359","DOI":"10.1128\/JB.187.10.3359-3368.2005","article-title":"Defective O-antigen polymerization in tolA and pal mutants of Escherichia coli in response to extracytoplasmic stress","volume":"187","author":"Vines","year":"2005","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.vetmic.2009.10.010_bib30","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1016\/j.micinf.2006.09.002","article-title":"Identification of Brucella melitensis 16M genes required for bacterial survival in the caprine host","volume":"8","author":"Zygmunt","year":"2006","journal-title":"Microb. Infect."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113509005306?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113509005306?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,23]],"date-time":"2020-05-23T03:00:40Z","timestamp":1590202840000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113509005306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5]]},"references-count":30,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["S0378113509005306"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2009.10.010","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2010,5]]}}}