{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T13:11:46Z","timestamp":1778591506585,"version":"3.51.4"},"reference-count":29,"publisher":"Elsevier BV","issue":"3-4","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"ANPCYT"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2014,8]]},"DOI":"10.1016\/j.vetmic.2014.04.014","type":"journal-article","created":{"date-parts":[[2014,4,30]],"date-time":"2014-04-30T18:32:06Z","timestamp":1398882726000},"page":"455-465","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"title":["A bacterial engineered glycoprotein as a novel antigen for diagnosis of bovine brucellosis"],"prefix":"10.1016","volume":"172","author":[{"given":"Andr\u00e9s E.","family":"Ciocchini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego A. Rey","family":"Serantes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciano J.","family":"Melli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia S.","family":"Guidolin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy A.","family":"Iwashkiw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebasti\u00e1n","family":"Elena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Franco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana M.","family":"Nicola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario F.","family":"Feldman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1949-4226","authenticated-orcid":false,"given":"Diego J.","family":"Comerci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan E.","family":"Ugalde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2014.04.014_bib0005","first-page":"577","article-title":"Laboratory-based diagnosis of brucellosis \u2013 a review of the literature. Part II: serological tests for brucellosis","volume":"49","author":"Al Dahouk","year":"2003","journal-title":"Clin. Lab."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0010","article-title":"Bovine brucellosis in Argentina and bordering countries: update","author":"Aznar","year":"2012","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0015","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1042\/bst0130980","article-title":"Structure and serology of the Brucella abortus O-antigen","volume":"13","author":"Bundle","year":"1985","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0020","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/j.1432-1033.1984.tb07994.x","article-title":"Structure of the O-chain of the phenol-phase soluble cellular lipopolysaccharide of Yersinia enterocolitica serotype O:9","volume":"139","author":"Caroff","year":"1984","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0025","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1128\/IAI.46.2.384-388.1984","article-title":"Antigenic S-type lipopolysaccharide of Brucella abortus 1119-3","volume":"46","author":"Caroff","year":"1984","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0030","doi-asserted-by":"crossref","first-page":"e2048","DOI":"10.1371\/journal.pntd.0002048","article-title":"Development and validation of a novel diagnostic test for human brucellosis using a glyco-engineered antigen coupled to magnetic beads","volume":"7","author":"Ciocchini","year":"2013","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0035","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3201\/eid0302.970219","article-title":"Brucellosis: an overview","volume":"3","author":"Corbel","year":"1997","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0040","series-title":"Manual de diagn\u00f3stico serol\u00f3gico de la brucellosis bovina versi\u00f3n 2","author":"DILAB-SENASA","year":"2009"},{"key":"10.1016\/j.vetmic.2014.04.014_bib0045","doi-asserted-by":"crossref","first-page":"3016","DOI":"10.1073\/pnas.0500044102","article-title":"Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli","volume":"102","author":"Feldman","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0050","doi-asserted-by":"crossref","first-page":"989","DOI":"10.20506\/rst.23.3.1545","article-title":"Serological diagnosis of bovine brucellosis: a review of test performance and cost comparison","volume":"23","author":"Gall","year":"2004","journal-title":"Rev. Sci. Tech."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0055","doi-asserted-by":"crossref","first-page":"296","DOI":"10.3325\/cmj.2010.51.296","article-title":"Diagnosis of brucellosis in livestock and wildlife","volume":"51","author":"Godfroid","year":"2010","journal-title":"Croat. Med. J."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0060","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0167-5877(00)00116-1","article-title":"Application of diagnostic tests in veterinary epidemiologic studies","volume":"45","author":"Greiner","year":"2000","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0065","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0022-1759(95)00121-P","article-title":"A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests","volume":"185","author":"Greiner","year":"1995","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.vetmic.2014.04.014_bib0070","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1148\/radiology.148.3.6878708","article-title":"A method of comparing the areas under receiver operating characteristic curves derived from the same cases","volume":"148","author":"Hanley","year":"1983","journal-title":"Radiology"},{"key":"10.1016\/j.vetmic.2014.04.014_bib0075","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1475-2859-11-13","article-title":"Exploiting the Campylobacter jejuni protein glycosylation system for glycoengineering vaccines and diagnostic tools directed against brucellosis","volume":"11","author":"Iwashkiw","year":"2012","journal-title":"Microb. Cell. Fact."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0080","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1016\/0264-410X(91)90010-4","article-title":"Induction of antibody and protective responses in mice by Brucella O-polysaccharide-BSA conjugate","volume":"9","author":"Jacques","year":"1991","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2014.04.014_bib0085","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.20506\/rst.25.3.1714","article-title":"Harmonisation of European tests for serological diagnosis of Brucella infection in bovines","volume":"25","author":"McGiven","year":"2006","journal-title":"Rev. Sci. Tech."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0090","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0022-1759(03)00201-1","article-title":"Validation of FPA and cELISA for the detection of antibodies to Brucella abortus in cattle sera and comparison to SAT, CFT, and iELISA","volume":"278","author":"McGiven","year":"2003","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.vetmic.2014.04.014_bib0095","doi-asserted-by":"crossref","first-page":"2820","DOI":"10.1128\/IAI.57.9.2820-2828.1989","article-title":"Fine structure of A and M antigens from Brucella biovars","volume":"57","author":"Meikle","year":"1989","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0100","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/0165-2427(90)90007-F","article-title":"The serological response of cattle immunized with Yersinia enterocolitica O:9 or O:16 to Yersinia and Brucella abortus antigens in enzyme immunoassays","volume":"24","author":"Nielsen","year":"1990","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0105","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0022-1759(96)00116-0","article-title":"A homogeneous fluorescence polarization assay for detection of antibody to Brucella abortus","volume":"195","author":"Nielsen","year":"1996","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.vetmic.2014.04.014_bib0110","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0165-2427(94)05361-U","article-title":"Improved competitive enzyme immunoassay for the diagnosis of bovine brucellosis","volume":"46","author":"Nielsen","year":"1995","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0115","series-title":"Manual of Diagnostic Tests and Vaccines for Terrestrial Animals","author":"OIE","year":"2012"},{"key":"10.1016\/j.vetmic.2014.04.014_bib0120","series-title":"Infectious and Parasitic Diseases of Livestock","first-page":"991","article-title":"Bovine brucellosis","author":"Saegerman","year":"2010"},{"key":"10.1016\/j.vetmic.2014.04.014_bib0125","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.vetmic.2009.06.021","article-title":"Brucellosis: a re-emerging zoonosis","volume":"140","author":"Seleem","year":"2010","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0130","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1126\/science.3287615","article-title":"Measuring the accuracy of diagnostic systems","volume":"240","author":"Swets","year":"1988","journal-title":"Science"},{"key":"10.1016\/j.vetmic.2014.04.014_bib0135","doi-asserted-by":"crossref","first-page":"6264","DOI":"10.1128\/IAI.71.11.6264-6269.2003","article-title":"Evaluation of Brucella abortus phosphoglucomutase (pgm) mutant as a new live rough-phenotype vaccine","volume":"71","author":"Ugalde","year":"2003","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0140","doi-asserted-by":"crossref","first-page":"5716","DOI":"10.1128\/IAI.68.10.5716-5723.2000","article-title":"Identification and characterization of the Brucella abortus phosphoglucomutase gene: role of lipopolysaccharide in virulence and intracellular multiplication","volume":"68","author":"Ugalde","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetmic.2014.04.014_bib0145","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1093\/clinids\/21.2.283","article-title":"An overview of human brucellosis","volume":"21","author":"Young","year":"1995","journal-title":"Clin. Infect. Dis."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113514002144?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113514002144?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T17:15:02Z","timestamp":1597770902000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113514002144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8]]},"references-count":29,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["S0378113514002144"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2014.04.014","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2014,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A bacterial engineered glycoprotein as a novel antigen for diagnosis of bovine brucellosis","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2014.04.014","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}