{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T05:43:22Z","timestamp":1789191802665,"version":"build-2803163510"},"reference-count":33,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100002850","name":"FONDECYT","doi-asserted-by":"publisher","award":["1130093"],"award-info":[{"award-number":["1130093"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agencia de Cooperaci\u00f3n Internacional de Chile (AGCI)"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2014,8]]},"DOI":"10.1016\/j.vetmic.2014.05.005","type":"journal-article","created":{"date-parts":[[2014,5,14]],"date-time":"2014-05-14T11:16:03Z","timestamp":1400066163000},"page":"279-284","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["Roles of genomic island 3 (GI-3) BAB1_0267 and BAB1_0270 open reading frames (ORFs) in the virulence of Brucella abortus 2308"],"prefix":"10.1016","volume":"172","author":[{"given":"Luisa","family":"Ortiz-Rom\u00e1n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Riquelme-Neira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"RobertoVidal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"O\u00f1ate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2014.05.005_bib0005","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0010","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1146\/annurev-micro-090110-102905","article-title":"Interactions of the human pathogenic Brucella species with their hosts","volume":"65","author":"Atluri","year":"2011","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0015","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/S0962-8924(99)01681-5","article-title":"No longer an exclusive club: eukaryotic signalling domains in bacteria","volume":"10","author":"Bakal","year":"2000","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0020","doi-asserted-by":"crossref","first-page":"36511","DOI":"10.1074\/jbc.M110.158055","article-title":"Src homology 3-interacting domain of Rv1917c of Mycobacterium tuberculosis induces selective maturation of human dendritic cells by regulating PI3K-MAPK-NF-kappaB signaling and drives Th2 immune responses","volume":"285","author":"Bansal","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0025","first-page":"231","volume":"Vol. 1","author":"Barrett","year":"2004"},{"key":"10.1016\/j.vetmic.2014.05.005_bib0030","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0092-8674(00)80966-6","article-title":"Structure of CheA, a signal-transducing histidine kinase","volume":"96","author":"Bilwes","year":"1999","journal-title":"Cell"},{"key":"10.1016\/j.vetmic.2014.05.005_bib0035","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1111\/j.1365-2958.2010.07383.x","article-title":"A M23B family metallopeptidase of Helicobacter pylori required for cell shape, pole formation and virulence","volume":"78","author":"Bonis","year":"2010","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0040","doi-asserted-by":"crossref","first-page":"e9435","DOI":"10.1371\/journal.pone.0009435","article-title":"An atypical riboflavin pathway is essential for Brucella abortus virulence","volume":"5","author":"Bonomi","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.vetmic.2014.05.005_bib0045","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1083\/jcb.200402073","article-title":"Cortactin and Crk cooperate to trigger actin polymerization during Shigella invasion of epithelial cells","volume":"166","author":"Bougn\u00e8res","year":"2004","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0050","doi-asserted-by":"crossref","first-page":"8197","DOI":"10.1128\/JB.01069-08","article-title":"Phosphatidylethanolamine synthesis is required for optimal virulence of Brucella abortus","volume":"190","author":"Bukata","year":"2008","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0055","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2011.09.025","article-title":"Roles of genomic island 3 (GI-3) BAB1_0278 and BAB1_0263 open reading frames (ORFs) in the virulence of Brucella abortus in BALB\/c mice","volume":"156","author":"C\u00e9spedes","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0060","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3201\/eid0302.970219","article-title":"Brucellosis: an overview","volume":"3","author":"Corbel","year":"1997","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0065","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"Datsenko","year":"2000","journal-title":"Proc. Nat. Acad. Sci. U.S.A."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0070","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.gene.2006.04.018","article-title":"A set of recombineering plasmids for gram-negative bacteria","volume":"379","author":"Datta","year":"2006","journal-title":"Gene"},{"key":"10.1016\/j.vetmic.2014.05.005_bib0075","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-1097(03)00963-7","article-title":"Brucella pathogenesis, genes identified from random large-scale screens","volume":"231","author":"Delrue","year":"2004","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0080","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1042\/BJ20101650","article-title":"Interaction between the SH3 domain of Src family kinases and the proline-rich motif of HTLV-1 p13: a novel mechanism underlying delivery of Src family kinases to mitochondria","volume":"439","author":"Elena","year":"2011","journal-title":"Biochem. J."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0085","doi-asserted-by":"crossref","first-page":"3629","DOI":"10.4049\/jimmunol.1202817","article-title":"Critical role of ASC inflammasomes and bacterial type IV secretion system in caspase-1 activation and host innate resistance to Brucella abortus infection","volume":"190","author":"Gomes","year":"2013","journal-title":"J. Immunol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0090","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0378-1135(02)00214-6","article-title":"Brucella intracellular life: from invasion to intracellular replication","volume":"90","author":"Gorvel","year":"2002","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0095","doi-asserted-by":"crossref","first-page":"12375","DOI":"10.1073\/pnas.192439399","article-title":"The two-component system BvrR\/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins with counterparts in members of the Rhizobiaceae","volume":"99","author":"Guzm\u00e1n-Verri","year":"2002","journal-title":"Proc. Nat. Acad. Sci. U.S.A."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0100","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.micpath.2012.09.002","article-title":"The role of a Brucella abortus lipoprotein in intracellular replication and pathogenicity in experimentally infected mice","volume":"54","author":"Kim","year":"2013","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0105","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1186\/1471-2164-11-300","article-title":"Proteomics-based confirmation of protein expression and correction of annotation errors in the Brucella abortus genome","volume":"11","author":"Lamontagne","year":"2010","journal-title":"BMC Genomics"},{"key":"10.1016\/j.vetmic.2014.05.005_bib0110","series-title":"Brucella: Molecular Microbiology and Genomics","first-page":"36","article-title":"The Brucella genomic islands","author":"Mancilla","year":"2011"},{"key":"10.1016\/j.vetmic.2014.05.005_bib0115","doi-asserted-by":"crossref","first-page":"3383","DOI":"10.1128\/JB.182.12.3383-3393.2000","article-title":"Salmonella enterica serovar typhimurium peptidase B is a leucyl aminopeptidase with specificity for acidic amino acids","volume":"182","author":"Mathew","year":"2000","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0125","doi-asserted-by":"crossref","first-page":"5040","DOI":"10.1128\/JB.186.15.5040-5051.2004","article-title":"Comparative whole-genome hybridization reveals genomic islands in Brucella species","volume":"186","author":"Rajashekara","year":"2004","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0130","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1471-2180-6-13","article-title":"Molecular targets for rapid identification of Brucella spp","volume":"6","author":"Ratushna","year":"2006","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0135","doi-asserted-by":"crossref","first-page":"4849","DOI":"10.1128\/JB.182.17.4849-4855.2000","article-title":"A homologue of an operon required for DNA transfer in Agrobacterium is required in Brucella abortus for virulence and intracellular multiplication","volume":"182","author":"Sieira","year":"2000","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0140","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1155\/2011\/518323","article-title":"Laboratory animal models for brucellosis research","volume":"2011","author":"Silva","year":"2011","journal-title":"J. Biomed. Biotechnol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0145","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.micinf.2010.10.015","article-title":"Cell-mediated immunity in human brucellosis","volume":"13","author":"Skendros","year":"2011","journal-title":"Microbes Infect."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0150","first-page":"119","article-title":"Metallopeptidases produced by group B Streptococcus: influence of proteolytic inhibitors on growth and on interaction with human cell lineages","volume":"22","author":"Soares","year":"2008","journal-title":"Int. J. Mol. Med."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0155","doi-asserted-by":"crossref","first-page":"221","DOI":"10.2174\/138920308784534014","article-title":"Extracellular SH3 domain containing proteins-features of a new protein family","volume":"9","author":"Stoll","year":"2008","journal-title":"Curr. Protein Pept. Sci."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0160","doi-asserted-by":"crossref","first-page":"5716","DOI":"10.1128\/IAI.68.10.5716-5723.2000","article-title":"Identification and characterization of the Brucella abortus phosphoglucomutase gene: role of lipopolysaccharide in virulence and intracellular multiplication","volume":"68","author":"Ugalde","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0165","doi-asserted-by":"crossref","first-page":"5614","DOI":"10.1128\/JB.00859-13","article-title":"A Zn-dependent metallopeptidase is responsible for sensitivity to LsbB, a class II leaderless bacteriocin of Lactococcus lactis subsp. lactis BGMN1-5","volume":"195","author":"Uzelac","year":"2013","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.vetmic.2014.05.005_bib0170","doi-asserted-by":"crossref","first-page":"4271","DOI":"10.1128\/IAI.00761-12","article-title":"Crucial role of gamma interferon-producing CD4+ Th1 cells but dispensable function of CD8+ T cell, B cell, Th2, and Th17 responses in the control of Brucella melitensis infection in mice","volume":"80","author":"Vitry","year":"2012","journal-title":"Infect. Immun."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113514002417?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113514002417?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,4]],"date-time":"2018-10-04T01:30:47Z","timestamp":1538616647000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113514002417"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8]]},"references-count":33,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["S0378113514002417"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2014.05.005","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2014,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Roles of genomic island 3 (GI-3) BAB1_0267 and BAB1_0270 open reading frames (ORFs) in the virulence of Brucella abortus 2308","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2014.05.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}