{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:29:09Z","timestamp":1789187349605,"version":"build-2803163510"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"2012 Texas A&M University"},{"name":"CONACYT: Collaborative research grant program"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1016\/j.vetmic.2017.10.020","type":"journal-article","created":{"date-parts":[[2017,10,27]],"date-time":"2017-10-27T20:32:45Z","timestamp":1509136365000},"page":"7-15","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Immunogenicity of a recombinant adenovirus expressing porcine reproductive and respiratory syndrome virus polyepitopes"],"prefix":"10.1016","volume":"212","author":[{"given":"Jes\u00fas","family":"Hern\u00e1ndez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar","family":"Rasc\u00f3n-Castelo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jocelyn","family":"Bray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shehnaz","family":"Lokhandwala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waithaka","family":"Mwangi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2017.10.020_bib0005","doi-asserted-by":"crossref","first-page":"663","DOI":"10.3390\/v5020663","article-title":"Predicted peptides from non-structural proteins of porcine reproductive and respiratory syndrome virus are able to induce IFN-gamma and IL-10","volume":"5","author":"Burgara-Estrella","year":"2013","journal-title":"Viruses"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0010","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.vetimm.2005.07.026","article-title":"Immune responses and protection by vaccine and various vaccine adjuvant candidates to virulent porcine reproductive and respiratory syndrome virus","volume":"109","author":"Charerntantanakul","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0015","doi-asserted-by":"crossref","first-page":"1838","DOI":"10.1016\/j.vaccine.2013.01.049","article-title":"Synthetic B- and T-cell epitope peptides of porcine reproductive and respiratory syndrome virus with Gp96 as adjuvant induced humoral and cell-mediated immunity","volume":"31","author":"Chen","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0020","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.jviromet.2006.03.012","article-title":"Comparison of RNA extraction methods for the detection of porcine reproductive and respiratory syndrome virus from boar semen","volume":"136","author":"Christopher-Hennings","year":"2006","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0025","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1177\/104063879200400201","article-title":"Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs","volume":"4","author":"Collins","year":"1992","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0030","doi-asserted-by":"crossref","first-page":"79","DOI":"10.3390\/genes8020079","article-title":"Transgene expression and host cell responses to replication-defective, single-cycle, and replication-competent adenovirus vectors","volume":"8","author":"Crosby","year":"2017","journal-title":"Genes"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0035","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.virol.2006.05.036","article-title":"Serologic marker candidates identified among B-cell linear epitopes of Nsp2 and structural proteins of a North American strain of porcine reproductive and respiratory syndrome virus","volume":"353","author":"de Lima","year":"2006","journal-title":"Virology"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0040","doi-asserted-by":"crossref","first-page":"3594","DOI":"10.1016\/j.vaccine.2008.04.078","article-title":"Development of a porcine reproductive and respiratory syndrome virus differentiable (DIVA) strain through deletion of specific immunodominant epitopes","volume":"26","author":"de Lima","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0045","doi-asserted-by":"crossref","first-page":"5603","DOI":"10.1016\/j.vaccine.2009.07.029","article-title":"In silico prediction and ex vivo evaluation of potential T-cell epitopes in glycoproteins 4 and 5 and nucleocapsid protein of genotype-I (European) of porcine reproductive and respiratory syndrome virus","volume":"27","author":"Diaz","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0050","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.jviromet.2007.12.018","article-title":"Enhanced immunogenicity of multiple-epitopes of foot-and-mouth disease virus fused with porcine interferon \u03b1 in mice and protective efficacy in guinea pigs and swine","volume":"149","author":"Du","year":"2008","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0055","doi-asserted-by":"crossref","first-page":"S73","DOI":"10.1038\/sj.gt.3302618","article-title":"Use of adenoviral vectors as veterinary vaccines","volume":"12","author":"Ferreira","year":"2005","journal-title":"Gene Ther."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0060","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1007\/s00705-002-0943-y","article-title":"Adenoviral-expressed GP5 of porcine respiratory and reproductive syndrome virus differs in its cellular maturation from the authentic viral protein but maintains known biological functions","volume":"148","author":"Gagnon","year":"2003","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0065","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1007\/s00705-002-0943-y","article-title":"Adenoviral-expressed GP5 of porcine respiratory and reproductive syndrome virus differs in its cellular maturation from the authentic viral protein but maintains known biological functions","volume":"148","author":"Gagnon","year":"2003","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0070","doi-asserted-by":"crossref","first-page":"72","DOI":"10.54846\/jshap\/754","article-title":"Assessment of the economic impact of porcine reproductive and respiratory syndrome virus on United States pork producers","volume":"21","author":"Holtkamp","year":"2013","journal-title":"J. Swine. Health. Prod."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0075","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.virol.2012.08.026","article-title":"Dissociation of porcine reproductive and respiratory syndrome virus neutralization from antibodies specific to major envelope protein surface epitopes","volume":"433","author":"Li","year":"2012","journal-title":"Virology"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0080","doi-asserted-by":"crossref","first-page":"4652","DOI":"10.1016\/S0264-410X(01)00233-X","article-title":"Optimization of epitope processing enhances immunogenicity of multiepitope DNA vaccines","volume":"19","author":"Livingston","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0085","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1128\/CVI.00395-16","article-title":"Induction of Robust Immune Responses in Swine by Using a Cocktail of Adenovirus-Vectored African Swine Fever Virus Antigens","volume":"23","author":"Lokhandwala","year":"2016","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0090","doi-asserted-by":"crossref","first-page":"6828","DOI":"10.1016\/j.vaccine.2014.04.054","article-title":"Proteome-wide screening of the European porcine reproductive and respiratory syndrome virus reveals a broad range of T cell antigen reactivity","volume":"32","author":"Mokhtar","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0095","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.vetmic.2017.01.037","article-title":"Evaluation of hydrophobic chitosan-based particulate formulations of porcine reproductive and respiratory syndrome virus vaccine candidate T cell antigens","volume":"209","author":"Mokhtar","year":"2017","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0100","doi-asserted-by":"crossref","first-page":"8382","DOI":"10.3390\/ijms16048382","article-title":"Production and evaluation of virus-like particles displaying immunogenic epitopes of porcine reproductive and respiratory syndrome virus (PRRSV)","volume":"16","author":"Murthy","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0105","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1016\/j.vaccine.2011.12.110","article-title":"CD205 antigen targeting combined with dendritic cell recruitment factors and antigen-linked CD40L activation primes and expands significant antigen-specific antibody and CD4+ T cell responses following DNA vaccination of outbred animals","volume":"30","author":"Njongmeta","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0110","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.jviromet.2005.05.017","article-title":"Discriminating between serological responses to European-genotype live vaccine and European-genotype field strains of porcine reproductive and respiratory syndrome virus (PRRSV) by peptide ELISA","volume":"129","author":"Oleksiewicz","year":"2005","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0115","doi-asserted-by":"crossref","first-page":"4241","DOI":"10.1128\/JVI.76.9.4241-4250.2002","article-title":"Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain","volume":"76","author":"Ostrowski","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0120","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.virusres.2012.06.024","article-title":"Location of T-cell epitopes in nonstructural proteins 9 and 10 of type-II porcine reproductive and respiratory syndrome virus","volume":"169","author":"Parida","year":"2012","journal-title":"Virus Res."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0125","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.virusres.2015.07.027","article-title":"Expression of antigenic epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) in a modified live-attenuated porcine circovirus type 2 (PCV2) vaccine virus (PCV1-2a) as a potential bivalent vaccine against both PCV2 and PRRSV","volume":"210","author":"Pi\u00f1eyro","year":"2015","journal-title":"Virus Res."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0130","doi-asserted-by":"crossref","first-page":"973","DOI":"10.3390\/vaccines3040973","article-title":"Immune response of multiparous hyper-immunized sows against peptides from non-structural and structural proteins of PRRSV","volume":"3","author":"Rascon-Castelo","year":"2015","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0135","doi-asserted-by":"crossref","first-page":"4069","DOI":"10.1016\/j.vaccine.2015.06.092","article-title":"Live porcine reproductive and respiratory syndrome virus vaccines: current status and future direction","volume":"33","author":"Renukaradhya","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0140","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1297-9716-44-17","article-title":"Immunogenic and protective properties of GP5 and M structural proteins of porcine reproductive and respiratory syndrome virus expressed from replicating but nondisseminating adenovectors","volume":"44","author":"Roques","year":"2013","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0145","doi-asserted-by":"crossref","first-page":"2141","DOI":"10.1099\/vir.0.056341-0","article-title":"Arterivirus molecular biology and pathogenesis","volume":"94","author":"Snijder","year":"2013","journal-title":"J. General Virol."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0150","doi-asserted-by":"crossref","first-page":"e34884","DOI":"10.1371\/journal.pone.0034884","article-title":"Pre-existing vector immunity does not prevent replication deficient adenovirus from inducing efficient CD8 T-cell memory and recall responses","volume":"7","author":"Steffensen","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0155","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.rvsc.2009.06.005","article-title":"Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus","volume":"88","author":"Sun","year":"2010","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0160","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.imlet.2010.09.010","article-title":"Comparison of the protective efficacy of recombinant adenoviruses against classical swine fever","volume":"135","author":"Sun","year":"2011","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0165","doi-asserted-by":"crossref","first-page":"4747","DOI":"10.1016\/j.vaccine.2008.06.047","article-title":"Identification of immunodominant T-cell epitopes present in glycoprotein 5 of the North American genotype of porcine reproductive and respiratory syndrome virus","volume":"26","author":"Vashisht","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0170","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.virusres.2011.03.018","article-title":"Identification of immunodominant T-cell epitopes in membrane protein of highly pathogenic porcine reproductive and respiratory syndrome virus","volume":"158","author":"Wang","year":"2011","journal-title":"Virus Res."},{"key":"10.1016\/j.vetmic.2017.10.020_bib0175","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1007\/s00705-014-2174-4","article-title":"Characterisation of novel linear antigen epitopes on North American-type porcine reproductive and respiratory syndrome virus M protein","volume":"159","author":"Wang","year":"2014","journal-title":"Arch. Virol."},{"issue":"Jun 7","key":"10.1016\/j.vetmic.2017.10.020_bib0180","article-title":"Prediction and in vitro verification of potential CTL epitopes conserved among PRRSV -2 strains","author":"Welner","year":"2017","journal-title":"Immunogenetics"},{"key":"10.1016\/j.vetmic.2017.10.020_bib0185","doi-asserted-by":"crossref","first-page":"3427","DOI":"10.1016\/j.vaccine.2004.02.040","article-title":"Protection of weaned pigs by vaccination with human adenovirus 5 recombinant viruses expressing the hemagglutinin and the nucleoprotein of H3N2 swine influenza virus","volume":"22","author":"Wesley","year":"2004","journal-title":"Vaccine"}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113517308441?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113517308441?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T19:39:59Z","timestamp":1750966799000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113517308441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":37,"alternative-id":["S0378113517308441"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2017.10.020","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Immunogenicity of a recombinant adenovirus expressing porcine reproductive and respiratory syndrome virus polyepitopes","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2017.10.020","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}