{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:05:57Z","timestamp":1790719557697,"version":"4.1.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T00:00:00Z","timestamp":1572307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Laboratorios Hipra S.A"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1016\/j.vetmic.2019.108480","type":"journal-article","created":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T11:40:03Z","timestamp":1573126803000},"page":"108480","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Experimental study for evaluation of the efficacy of a biofilm-embedded bacteria-based vaccine against Staphylococcus chromogenes-associated mastitis in sheep"],"prefix":"10.1016","volume":"239","author":[{"given":"N.G.C.","family":"Vasileiou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.J.","family":"Cripps","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.S.","family":"Ioannidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.I.","family":"Katsafadou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.C.","family":"Chatzopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.S.","family":"Barbagianni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Tsioli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Dermisiadou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Karavanis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Papadopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.T.","family":"Lianou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.S.","family":"Mavrogianni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Petinaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.C.","family":"Fthenakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2019.108480_bib0005","doi-asserted-by":"crossref","first-page":"125","DOI":"10.14202\/vetworld.2018.125-129","article-title":"Bacteriological cure rate and changes in milk composition in mastitis vaccinated ewes affected with subclinical mastitis","volume":"11","author":"Alekish","year":"2018","journal-title":"Vet. World"},{"key":"10.1016\/j.vetmic.2019.108480_bib0010","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/0264-410X(94)90201-1","article-title":"Use of liposome-immunopotentiated exopolysaccharide as a component of an ovine mastitis staphylococcal vaccine","volume":"12","author":"Amorena","year":"1994","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2019.108480_bib0015","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.smallrumres.2016.12.008","article-title":"Ultrasonographic examination of pregnant ewes: from early diagnosis of pregnancy to early prediction of dystocia","volume":"152","author":"Barbagianni","year":"2017","journal-title":"Small Rumin. Res."},{"key":"10.1016\/j.vetmic.2019.108480_bib0020","doi-asserted-by":"crossref","first-page":"S320","DOI":"10.1016\/S2222-1808(14)60463-1","article-title":"Histopathology of mammary gland in Staphylococcus aureus induced mastitis in mice","volume":"4","author":"Chinchali","year":"2014","journal-title":"Asian Pac. J. Trop. Dis."},{"key":"10.1016\/j.vetmic.2019.108480_bib0025","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0065-2911(06)51004-5","article-title":"Surface adhesins of Staphylococcus aureus","volume":"51","author":"Clarke","year":"2006","journal-title":"Adv. Microbial Physiol."},{"key":"10.1016\/j.vetmic.2019.108480_bib0030","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1590\/0103-8478cr20150365","article-title":"Qualitative and quantitative analysis of bacteria from vaginitis associated with intravaginal implants in ewes following estrus synchronization","volume":"46","author":"de Paula Vasconcelos","year":"2016","journal-title":"Cienc. Rural"},{"key":"10.1016\/j.vetmic.2019.108480_bib0035","first-page":"3933","article-title":"Scientific opinion on the welfare risks related to the farming of sheep for wool, meat and milk production","volume":"12","author":"European Food Safety Authority","year":"2014","journal-title":"EFSA J."},{"key":"10.1016\/j.vetmic.2019.108480_bib0040","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.smallrumres.2013.12.015","article-title":"Diagnosis of clinical or subclinical mastitis in ewes","volume":"118","author":"Fragkou","year":"2014","journal-title":"Small Rumin. Res."},{"key":"10.1016\/j.vetmic.2019.108480_bib0045","first-page":"117","article-title":"Pathogenicity for mice of coagulase-negative staphylococci, isolated from cases of ovine mastitis","volume":"43","author":"Fthenakis","year":"1992","journal-title":"Bull. Hell. Vet. Med. Soc."},{"key":"10.1016\/j.vetmic.2019.108480_bib0050","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.anireprosci.2012.01.016","article-title":"Health management of ewes during pregnancy","volume":"130","author":"Fthenakis","year":"2012","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.vetmic.2019.108480_bib0055","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/0007-1935(90)90075-E","article-title":"The effect of experimentally induced subclinical mastitis on the milk yield of ewes and on the growth of lambs","volume":"146","author":"Fthenakis","year":"1990","journal-title":"Br. Vet. J."},{"key":"10.1016\/j.vetmic.2019.108480_bib0060","series-title":"Ovine Mastitis With Special Reference To Subclinical Mastitis Associated With Coagulase-negative Staphylococci. PhD Thesis","author":"Fthenakis","year":"1988"},{"key":"10.1016\/j.vetmic.2019.108480_bib0065","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.vetmic.2015.07.009","article-title":"Mastitis in sheep \u2013 the last 10 years and the future of research","volume":"185","author":"Gelasakis","year":"2015","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2019.108480_bib0070","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0061319","article-title":"Within-host evolution of Staphylococcus aureus during asymptomatic carriage","volume":"8","author":"Golubchik","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.vetmic.2019.108480_bib0075","first-page":"168","article-title":"Recommended methods for somatic cell counting in milk","author":"International Dairy Federation","year":"1984","journal-title":"Bul. Int. Dairy Fed."},{"key":"10.1016\/j.vetmic.2019.108480_bib0080","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1097\/00003086-199706000-00033","article-title":"New model for chronic osteomyelitis with Staphylococcus aureus in sheep","volume":"339","author":"Kaarsemaker","year":"1997","journal-title":"Clin. Orthopaed. Relat. Res."},{"key":"10.1016\/j.vetmic.2019.108480_bib0085","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.vetmic.2015.07.018","article-title":"Vaccination schedules in small ruminant farms","volume":"181","author":"Lacasta","year":"2015","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2019.108480_bib0090","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0928-8244(02)00458-3","article-title":"Staphylococcus aureus strains isolated from bovine mastitis: virulence, antibody production and protection from challenge in a mouse model","volume":"35","author":"Leitner","year":"2003","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1016\/j.vetmic.2019.108480_bib0095","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.vetimm.2011.03.023","article-title":"Vaccine development for the prevention of staphylococcal mastitis in dairy cows","volume":"142","author":"Leitner","year":"2011","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetmic.2019.108480_bib0100","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0165-2427(03)00051-5","article-title":"Development of a Staphylococcus aureus vaccine against mastitis in dairy cows. I. Challenge trials","volume":"93","author":"Leitner","year":"2003","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetmic.2019.108480_bib0105","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0165-2427(03)00062-X","article-title":"Development of a Staphylococcus aureus vaccine against mastitis in dairy cows. II. Field trial","volume":"93","author":"Leitner","year":"2003","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetmic.2019.108480_bib0110","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1051\/vetres:2004048","article-title":"The effects of inoculation of Mannheimia haemolytica into the teat of lactating ewes","volume":"36","author":"Mavrogianni","year":"2005","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetmic.2019.108480_bib0115","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1051\/vetres:2005042","article-title":"Teat disorders predispose ewes to clinical mastitis after challenge with Mannheimia haemolytica","volume":"37","author":"Mavrogianni","year":"2006","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetmic.2019.108480_bib0120","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.tvjl.2005.01.006","article-title":"Biofilms: a role in recurrent mastitis infections?","volume":"171","author":"Melchior","year":"2006","journal-title":"Vet. J."},{"key":"10.1016\/j.vetmic.2019.108480_bib0125","first-page":"732","article-title":"The estimation of the bactericidal power of the blood","volume":"38","author":"Miles","year":"1938","journal-title":"J. Hygiene Cambridge"},{"key":"10.1016\/j.vetmic.2019.108480_bib0130","series-title":"Image J: Image Processing and Analysis in Java","author":"National Institutes of Health","year":"2013"},{"key":"10.1016\/j.vetmic.2019.108480_bib0135","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1109\/TPAMI.2002.1017623","article-title":"Multiresolution gray-scale and rotation invariant texture classification with local binary patterns","volume":"24","author":"Ojala","year":"2002","journal-title":"IEEE Trans. Pattern Anal. Mach. Intell."},{"key":"10.1016\/j.vetmic.2019.108480_bib0140","first-page":"207","article-title":"Staphylococcal biofilms","volume":"322","author":"Otto","year":"2008","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1016\/j.vetmic.2019.108480_bib0145","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1016\/j.vaccine.2009.02.005","article-title":"Protection from Staphylococcus aureus mastitis associated with poly- N- acetyl \u03b2-1,6 glycosamine specific antibody production using biofilm- embedded bacteria","volume":"27","author":"Perez","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2019.108480_bib0150","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1017\/S0022029913000472","article-title":"Administration of antibiotics to ewes at the beginning of the dry-period","volume":"81","author":"Petridis","year":"2014","journal-title":"J. Dairy Res."},{"key":"10.1016\/j.vetmic.2019.108480_bib0155","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.4161\/hv.25182","article-title":"Will there ever be a universal Staphylococcus aureus vaccine?","volume":"9","author":"Pier","year":"2013","journal-title":"Hum. Vacc. Immunother."},{"key":"10.1016\/j.vetmic.2019.108480_bib0160","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.vetimm.2009.09.020","volume":"134","author":"Prenafeta","year":"2010","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetmic.2019.108480_bib0165","doi-asserted-by":"crossref","DOI":"10.3168\/jds.2019-16287","article-title":"Evaluation of efficacy of a biofilm-embedded bacteria-based vaccine against staphylococcal mastitis in sheep\u2014a randomized, placebo-controlled field study","author":"Vasileiou","year":"2019","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.vetmic.2019.108480_bib0170","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.vetmic.2018.08.022","article-title":"Slime-producing staphylococci as causal agents of subclinical mastitis in sheep","volume":"224","author":"Vasileiou","year":"2018","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2019.108480_bib0175","doi-asserted-by":"crossref","first-page":"7297","DOI":"10.3168\/jds.2017-14075","article-title":"Extensive countrywide field investigation of subclinical mastitis in sheep in Greece","volume":"101","author":"Vasileiou","year":"2018","journal-title":"J. Dairy Sci."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113519305619?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113519305619?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T23:08:44Z","timestamp":1759878524000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113519305619"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12]]},"references-count":35,"alternative-id":["S0378113519305619"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2019.108480","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2019,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Experimental study for evaluation of the efficacy of a biofilm-embedded bacteria-based vaccine against Staphylococcus chromogenes-associated mastitis in sheep","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2019.108480","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"108480"}}