{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:25:03Z","timestamp":1793118303744,"version":"4.5.0"},"reference-count":23,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,4,17]],"date-time":"2020-04-17T00:00:00Z","timestamp":1587081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011019","name":"Nemzeti Kutat\u00e1si Fejleszt\u00e9si \u00e9s Innov\u00e1ci\u00f3s Hivatal","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011019","name":"Nemzeti Kutat\u00e1si Fejleszt\u00e9si \u00e9s Innov\u00e1ci\u00f3s Hivatal","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003825","name":"Magyar Tudom\u00e1nyos Akad\u00e9mia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1016\/j.vetmic.2020.108697","type":"journal-article","created":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T10:50:39Z","timestamp":1588330239000},"page":"108697","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Development of molecular biological tools for the rapid determination of antibiotic susceptibility of Mycoplasma hyopneumoniae isolates"],"prefix":"10.1016","volume":"245","author":[{"given":"Orsolya","family":"Felde","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsuzsa","family":"Kreizinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kinga Maria","family":"Sulyok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eniko","family":"Wehmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miklos","family":"Gyuranecz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2020.108697_bib0005","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0032866","article-title":"Melt analysis of mismatch amplification mutation assays (melt-MAMA): a functional study of a cost-effective SNP genotyping assay in bacterial models","volume":"7","author":"Birdsell","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.vetmic.2020.108697_bib0010","first-page":"1","article-title":"Antibiotic susceptibility testing of Mycoplasma hyopneumoniae field isolates from Central Europe for fifteen antibiotics by microbroth dilution method","author":"Felde","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.vetmic.2020.108697_bib0015","first-page":"337","article-title":"Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey","volume":"27","author":"Friis","year":"1975","journal-title":"Nord. Vet."},{"key":"10.1016\/j.vetmic.2020.108697_bib0020","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/microbiolspec.ARBA-0030-2018","article-title":"Antimicrobial resistance in Mycoplasma spp","volume":"6","author":"Gautier-Bouchardon","year":"2018","journal-title":"Microbiol. Spectrum"},{"key":"10.1016\/j.vetmic.2020.108697_bib0025","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1128\/AAC.41.9.2037","article-title":"Comparative susceptibilities of various animal-pathogenic mycoplasmas to fluoroquinolones","author":"Hannan","year":"1997","journal-title":"Antimicrob. Agents"},{"key":"10.1016\/j.vetmic.2020.108697_bib0030","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1051\/vetres:2000100","article-title":"Guidelines and recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary mycoplasma species","volume":"31","author":"Hannan","year":"2000","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetmic.2020.108697_bib0035","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1128\/AAC.01527-05","article-title":"Persistence of Mycoplasma hyopneumoniae in experimentally infected pigs after marbofloxacin treatment and detection of mutations in the parC gene","author":"Le Carrou","year":"2006","journal-title":"Antimicrob. Agents"},{"key":"10.1016\/j.vetmic.2020.108697_bib0040","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.vetmic.2007.09.008","article-title":"Control of Mycoplasma hyopneumoniae infections in pigs","volume":"126","author":"Maes","year":"2008","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2020.108697_bib0045","first-page":"1","article-title":"Update on Mycoplasma hyopneumoniae infections in pigs: knowledge gaps for improved disease control","author":"Maes","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.vetmic.2020.108697_bib0050","first-page":"104","article-title":"Enzootic pneumonia in pigs","volume":"18","author":"Maes","year":"1996","journal-title":"Vet. Res. Forum"},{"key":"10.1016\/j.vetmic.2020.108697_bib0055","doi-asserted-by":"crossref","first-page":"7081","DOI":"10.1016\/j.vaccine.2006.07.004","article-title":"Comparison of transmission of Mycoplasma hyopneumoniae in vaccinated and non-vaccinated populations","volume":"24","author":"Meyns","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2020.108697_bib0060","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.prevetmed.2004.10.001","article-title":"Quantification of the spread of Mycoplasma hyopneumoniae in nursery pigs using transmission experiments","volume":"66","author":"Meyns","year":"2004","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.vetmic.2020.108697_bib0065","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.ab.2005.08.010","article-title":"Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping","volume":"346","author":"Palais","year":"2005","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.vetmic.2020.108697_bib0070","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1136\/vetreco-2014-000079","article-title":"Use of tylvalosin in the control of porcine enzootic pneumonia","volume":"2","author":"Pallar\u00e9s","year":"2015","journal-title":"Vet. Rec. Open"},{"key":"10.1016\/j.vetmic.2020.108697_bib0075","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/S0165-6147(00)01717-X","article-title":"Genetic susceptibility to adverse drug reactions","volume":"22","author":"Pirmohamed","year":"2001","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/j.vetmic.2020.108697_bib0080","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.prevetmed.2019.04.008","article-title":"Benefit-cost analysis to estimate the payback time and the economic value of two Mycoplasma hyopneumoniae elimination methods in breeding herds","volume":"168","author":"Silva","year":"2019","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.vetmic.2020.108697_bib0085","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1089\/mdr.2005.11.290","article-title":"Characterization of in vivo acquired resistance of Mycoplasma hyopneumoniae to macrolides and lincosamides","volume":"11","author":"Stakenborg","year":"2005","journal-title":"Microb. Drug Resist."},{"key":"10.1016\/j.vetmic.2020.108697_bib0090","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.mimet.2005.12.002","article-title":"Comparison of molecular techniques for the typing of Mycoplasma hyopneumoniae isolates","volume":"66","author":"Stakenborg","year":"2006","journal-title":"J. Microbiol. Meth."},{"key":"10.1016\/j.vetmic.2020.108697_bib0095","series-title":"Calculator for Determining the Number of Copies of a Template","author":"Staroscik","year":"2004"},{"key":"10.1016\/j.vetmic.2020.108697_bib0100","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.vetmic.2017.11.026","article-title":"Development of molecular methods for the rapid detection of antibiotic susceptibility of Mycoplasma bovis","volume":"213","author":"Sulyok","year":"2018","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2020.108697_bib0105","first-page":"107","article-title":"Comparison of antibody production, lymphocyte stimulation, and protection induced by four commercial Mycoplasma hyopneumoniae bacterins","volume":"6","author":"Thacker","year":"1998","journal-title":"Swine Health Prod."},{"key":"10.1016\/j.vetmic.2020.108697_bib0110","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1089\/mdr.2007.716","article-title":"Resistance mechanism against fluoroquinolones in Mycoplasma hyopneumoniae field isolates","volume":"13","author":"Vicca","year":"2007","journal-title":"Microb. Drug Resist."},{"key":"10.1016\/j.vetmic.2020.108697_bib0115","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1016\/j.vaccine.2008.12.005","article-title":"Infection with a low virulent Mycoplasma hyopneumoniae isolate does not protect piglets against subsequent infection with a highly virulent M. Hyopneumoniae isolate","volume":"27","author":"Villarreal","year":"2009","journal-title":"Vaccine"}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113520302704?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113520302704?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T23:08:31Z","timestamp":1759878511000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113520302704"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":23,"alternative-id":["S0378113520302704"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2020.108697","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2020,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of molecular biological tools for the rapid determination of antibiotic susceptibility of Mycoplasma hyopneumoniae isolates","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2020.108697","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"108697"}}