{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:30:23Z","timestamp":1779208223886,"version":"3.51.4"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100007135","name":"University of Tennessee","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007135","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2020,7]]},"DOI":"10.1016\/j.vetmic.2020.108720","type":"journal-article","created":{"date-parts":[[2020,5,16]],"date-time":"2020-05-16T02:51:16Z","timestamp":1589597476000},"page":"108720","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["Staphylococcus pseudintermedius 5\u2032-nucleotidase suppresses canine phagocytic activity"],"prefix":"10.1016","volume":"246","author":[{"given":"Mohamed A.","family":"Abouelkhair","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda A.","family":"Frank","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Bemis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J.","family":"Giannone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen A.","family":"Kania","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"7","key":"10.1016\/j.vetmic.2020.108720_bib0005","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.01651-16","article-title":"Complete genome sequence of Staphylococcus pseudintermedius type strain LMG 22219","volume":"5","author":"Abouelkhair","year":"2017","journal-title":"Genome Announc."},{"key":"10.1016\/j.vetmic.2020.108720_bib0010","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0204450","article-title":"Characterization of a leukocidin identified in Staphylococcus pseudintermedius","volume":"13","author":"Abouelkhair","year":"2018","journal-title":"PLoS ONE"},{"key":"10.1016\/j.vetmic.2020.108720_bib0015","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1080\/21505594.2018.1489199","article-title":"Characterization of recombinant wild-type and nontoxigenic protein A from Staphylococcus pseudintermedius","volume":"9","author":"Abouelkhair","year":"2018","journal-title":"Virulence"},{"issue":"10","key":"10.1016\/j.vetmic.2020.108720_bib0020","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.01599-17","article-title":"Complete genome sequences of three Staphylococcus pseudintermedius strains isolated from Botswana","volume":"6","author":"Abouelkhair","year":"2018","journal-title":"Genome Announc."},{"key":"10.1016\/j.vetmic.2020.108720_bib0025","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0220301","article-title":"Identification, cloning and characterization of SpEX exotoxin produced by Staphylococcus pseudintermedius","volume":"14","author":"Abouelkhair","year":"2019","journal-title":"PLoS ONE"},{"key":"10.1016\/j.vetmic.2020.108720_bib0030","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1080\/21505594.2017.1403710","article-title":"Expression and function of protein A in Staphylococcus pseudintermedius","volume":"9","author":"Balachandran","year":"2018","journal-title":"Virulence"},{"key":"10.1016\/j.vetmic.2020.108720_bib0035","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1161\/ATVBAHA.111.226845","article-title":"Regulation of neutrophil function by adenosine","volume":"32","author":"Barletta","year":"2012","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.vetmic.2020.108720_bib0040","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.pharmthera.2005.04.013","article-title":"Adenosine 5\u2019-triphosphate and adenosine as endogenous signaling molecules in immunity and inflammation","volume":"112","author":"Bours","year":"2006","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.vetmic.2020.108720_bib0045","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.4049\/jimmunol.148.7.2201","article-title":"Neutrophil adherence to endothelium is enhanced via adenosine A1 receptors and inhibited via adenosine A2 receptors","volume":"148","author":"Cronstein","year":"1992","journal-title":"J. Immunol."},{"key":"10.1016\/j.vetmic.2020.108720_bib0050","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00362-19","article-title":"Broad and effective protection against Staphylococcus aureus is elicited by a multivalent vaccine formulated with novel antigens","volume":"4","author":"Deng","year":"2019","journal-title":"mSphere"},{"key":"10.1016\/j.vetmic.2020.108720_bib0055","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/s11302-005-6312-z","article-title":"Purinergic mechanism in the immune system: a signal of danger for dendritic cells","volume":"1","author":"Di Virgilio","year":"2005","journal-title":"Purinergic Signal"},{"key":"10.1016\/j.vetmic.2020.108720_bib0060","doi-asserted-by":"crossref","DOI":"10.3390\/ijms19041222","article-title":"Mechanisms of ATP release by inflammatory cells","volume":"19","author":"Dosch","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.vetmic.2020.108720_bib0065","doi-asserted-by":"crossref","first-page":"4141","DOI":"10.4049\/jimmunol.143.12.4141","article-title":"Adenosine receptors are expressed during differentiation of monocytes to macrophages in vitro. Implications for regulation of phagocytosis","volume":"143","author":"Eppell","year":"1989","journal-title":"J. Immunol."},{"key":"10.1016\/j.vetmic.2020.108720_bib0070","doi-asserted-by":"crossref","first-page":"5479","DOI":"10.1074\/jbc.M113.545632","article-title":"Extracellular nucleotide catabolism by the Group B Streptococcus ectonucleotidase NudP increases bacterial survival in blood","volume":"289","author":"Firon","year":"2014","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetmic.2020.108720_bib0075","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1111\/j.1365-3164.2009.00828.x","article-title":"The Staphylococcus intermedius group of bacterial pathogens: species re-classification, pathogenesis and the emergence of meticillin resistance","volume":"20","author":"Fitzgerald","year":"2009","journal-title":"Vet. Dermatol."},{"key":"10.1016\/j.vetmic.2020.108720_bib0080","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1038\/nrmicro1289","article-title":"Immune evasion by staphylococci","volume":"3","author":"Foster","year":"2005","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.vetmic.2020.108720_bib0085","doi-asserted-by":"crossref","first-page":"2962","DOI":"10.1016\/j.vaccine.2016.03.110","article-title":"Status of vaccine research and development of vaccines for Staphylococcus aureus","volume":"34","author":"Giersing","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2020.108720_bib0090","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1189\/jlb.0607359","article-title":"A2A receptors in inflammation and injury: lessons learned from transgenic animals","volume":"83","author":"Hasko","year":"2008","journal-title":"J. Leukoc. Biol."},{"key":"10.1016\/j.vetmic.2020.108720_bib0095","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/S0022-3565(24)36369-4","article-title":"Adenosine A2 receptor activation attenuates reperfusion injury by inhibiting neutrophil accumulation, superoxide generation and coronary endothelial adherence","volume":"280","author":"Jordan","year":"1997","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.vetmic.2020.108720_bib0100","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data","volume":"28","author":"Kearse","year":"2012","journal-title":"Bioinformatics"},{"key":"10.1016\/j.vetmic.2020.108720_bib0105","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.vetmic.2020.108720_bib0110","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1093\/infdis\/jiu050","article-title":"Streptococcus suis adenosine synthase functions as an effector in evasion of PMN-mediated innate immunit","volume":"210","author":"Liu","year":"2014","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.vetmic.2020.108720_bib0115","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1093\/femsre\/fuz030","article-title":"Development of a vaccine against Staphylococcus aureus invasive infections: evidence based on human immunity, genetics and bacterial evasion mechanisms","volume":"44","author":"Miller","year":"2020","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.vetmic.2020.108720_bib0120","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/0002-8703(92)90643-A","article-title":"The effects of intravenous infusions of selective adenosine A1-receptor and A2-receptor agonists on myocardial reperfusion injury","volume":"123","author":"Norton","year":"1992","journal-title":"Am. Heart J."},{"key":"10.1016\/j.vetmic.2020.108720_bib0125","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1093\/jac\/dkq078","article-title":"Clonal spread of methicillin-resistant Staphylococcus pseudintermedius in Europe and North America: an international multicentre study","volume":"65","author":"Perreten","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.vetmic.2020.108720_bib0130","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S0031-6997(24)01373-5","article-title":"Receptors for purines and pyrimidines","volume":"50","author":"Ralevic","year":"1998","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.vetmic.2020.108720_bib0135","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s00281-011-0295-3","article-title":"Neutrophils in innate host defense against Staphylococcus aureus infections","volume":"34","author":"Rigby","year":"2012","journal-title":"Semin. Immunopathol."},{"key":"10.1016\/j.vetmic.2020.108720_bib0140","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.01194-16","article-title":"Complete genome sequences of three important methicillin-resistant clinical isolates of Staphylococcus pseudintermedius","volume":"4","author":"Riley","year":"2016","journal-title":"Genome Announc."},{"key":"10.1016\/j.vetmic.2020.108720_bib0145","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1097\/00005344-199307000-00015","article-title":"Adenosine A2-receptor activation at reperfusion reduces infarct size and improves myocardial wall function in dog heart","volume":"22","author":"Schlack","year":"1993","journal-title":"J. Cardiovasc. Pharmacol."},{"key":"10.1016\/j.vetmic.2020.108720_bib0150","doi-asserted-by":"crossref","first-page":"153","DOI":"10.4252\/wjsc.v6.i2.153","article-title":"Purinergic receptors and nucleotide processing ectoenzymes: their roles in regulating mesenchymal stem cell functions","volume":"6","author":"Scarfi","year":"2014","journal-title":"World J. Stem Cells"},{"key":"10.1016\/j.vetmic.2020.108720_bib0155","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1128\/JCM.02421-12","article-title":"Multilocus sequence typing for characterization of Staphylococcus pseudintermedius","volume":"51","author":"Solyman","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetmic.2020.108720_bib0160","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1084\/jem.20090097","article-title":"Staphylococcus aureus synthesizes adenosine to escape host immune responses","volume":"206","author":"Thammavongsa","year":"2009","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.vetmic.2020.108720_bib0165","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/1471-2091-12-56","article-title":"Enzymatic properties of Staphylococcus aureus adenosine synthase (AdsA)","volume":"12","author":"Thammavongsa","year":"2011","journal-title":"BMC Biochem."},{"key":"10.1016\/j.vetmic.2020.108720_bib0170","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S1286-4579(03)00068-6","article-title":"The critical role of adenosine A2A receptors in downregulation of inflammation and immunity in the pathogenesis of infectious diseases","volume":"5","author":"Thiel","year":"2003","journal-title":"Microbes Infect."},{"key":"10.1016\/j.vetmic.2020.108720_bib0175","article-title":"Clonal complexes and antimicrobial susceptibility profiles of Staphylococcus pseudintermedius isolates from dogs in the United States","author":"Videla","year":"2017","journal-title":"Microb. Drug Resist."},{"key":"10.1016\/j.vetmic.2020.108720_bib0180","doi-asserted-by":"crossref","first-page":"W469","DOI":"10.1093\/nar\/gkq406","article-title":"3DLigandSite: predicting ligand-binding sites using similar structures","volume":"38","author":"Wass","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.vetmic.2020.108720_bib0185","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.vetmic.2009.01.039","article-title":"Methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in veterinary medicine","volume":"140","author":"Weese","year":"2010","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2020.108720_bib0190","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1093\/bioinformatics\/btq249","article-title":"PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes","volume":"26","author":"Yu","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.vetmic.2020.108720_bib0195","doi-asserted-by":"crossref","first-page":"31126","DOI":"10.1074\/jbc.M115.677443","article-title":"Streptococcal 5\u2019-Nucleotidase a (S5nA), a novel Streptococcus pyogenes virulence factor that facilitates immune evasion","volume":"290","author":"Zheng","year":"2015","journal-title":"J. Biol. Chem."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113520303680?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113520303680?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T23:08:27Z","timestamp":1759878507000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113520303680"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7]]},"references-count":39,"alternative-id":["S0378113520303680"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2020.108720","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2020,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Staphylococcus pseudintermedius 5\u2032-nucleotidase suppresses canine phagocytic activity","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2020.108720","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"108720"}}