{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T05:34:09Z","timestamp":1780378449305,"version":"3.54.1"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Zhejiang Province Natural Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2021,3]]},"DOI":"10.1016\/j.vetmic.2021.109012","type":"journal-article","created":{"date-parts":[[2021,2,6]],"date-time":"2021-02-06T20:50:49Z","timestamp":1612644649000},"page":"109012","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Regulated delayed attenuation improves vaccine efficacy in preventing infection from avian pathogenic Escherichia coli O78 and Salmonella typhimurium"],"prefix":"10.1016","volume":"254","author":[{"given":"Yue","family":"Han","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiyu","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mianmian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueyue","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruidong","family":"Zhai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyong","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houhui","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2021.109012_bib0005","series-title":"A Complete View of the Escherichia coli O-antigen Biosynthesis Gene Cluster and the Development of Molecular-based O-serogrouping Methods","author":"Atsushi","year":"2016"},{"key":"10.1016\/j.vetmic.2021.109012_bib0010","doi-asserted-by":"crossref","first-page":"39","DOI":"10.4049\/jimmunol.1600608","article-title":"Salmonella vaccines: conduits for protective antigens","volume":"200","author":"Clark-Curtiss","year":"2018","journal-title":"J. Immunol."},{"issue":"Suppl","key":"10.1016\/j.vetmic.2021.109012_bib0015","doi-asserted-by":"crossref","first-page":"114s","DOI":"10.1046\/j.1365-2672.94.s1.13.x","article-title":"The rise and fall of Salmonella enteritidisin the UK","volume":"94","author":"Cogan","year":"2003","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109012_bib0020","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1186\/s12864-016-3289-7","article-title":"Comparative genomics of European avian pathogenic E. Coli (APEC)","volume":"17","author":"Cordoni","year":"2016","journal-title":"BMC Genomics"},{"key":"10.1016\/j.vetmic.2021.109012_bib0025","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1128\/IAI.00693-08","article-title":"Salmonella enterica serovar typhimurium strains with regulated delayed attenuation in vivo","volume":"77","author":"Curtiss","year":"2009","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetmic.2021.109012_bib0030","article-title":"Comparison of O-Antigen gene clusters of all O-Serogroups of Escherichia coli and proposal for adopting a new nomenclature for O-Typing","volume":"11","author":"DebRoy","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.vetmic.2021.109012_bib0035","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15585\/mmwr.ss6710a1","article-title":"Surveillance for foodborne disease outbreaks - United States, 2009-2015. Morbidity and mortality weekly report","volume":"67","author":"Dewey-Mattia","year":"2018","journal-title":"Surveillance Summaries (Washington, D.C.: 2002)"},{"key":"10.1016\/j.vetmic.2021.109012_bib0040","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.vetmic.2014.04.019","article-title":"Advances in vaccination against avian pathogenic Escherichia coli respiratory disease: potentials and limitations","volume":"172","author":"Ghunaim","year":"2014","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109012_bib0045","doi-asserted-by":"crossref","DOI":"10.1093\/femspd\/ftx102","article-title":"A biologically conjugated polysaccharide vaccine delivered by attenuated Salmonella typhimurium provides protection against challenge of avian pathogenic Escherichia coli O1 infection","volume":"75","author":"Han","year":"2017","journal-title":"Pathog. Dis."},{"key":"10.1016\/j.vetmic.2021.109012_bib0050","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1016\/j.vaccine.2018.01.036","article-title":"A bivalent vaccine derived from attenuated Salmonella expressing O-antigen polysaccharide provides protection against avian pathogenic Escherichia coli O1 and O2 infection","volume":"36","author":"Han","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2021.109012_bib0055","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.vetmic.2018.12.020","article-title":"Use of a modified bacterial ghost lysis system for the construction of an inactivated avian pathogenic Escherichia coli vaccine candidate","volume":"229","author":"Hu","year":"2019","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109012_bib0060","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/s12934-020-01372-7","article-title":"Exploiting bacterial outer membrane vesicles as a cross-protective vaccine candidate against avian pathogenic Escherichia coli (APEC)","volume":"19","author":"Hu","year":"2020","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.vetmic.2021.109012_bib0065","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1016\/j.psj.2019.10.047","article-title":"Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis","volume":"99","author":"Kim","year":"2020","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetmic.2021.109012_bib0070","doi-asserted-by":"crossref","first-page":"3116","DOI":"10.1128\/IAI.68.6.3116-3120.2000","article-title":"Effect of preexisting immunity to Salmonella on the immune response to recombinant Salmonella enterica serovar typhimurium expressing a Porphyromonas gingivalis hemagglutinin","volume":"68","author":"Kohler","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetmic.2021.109012_bib0075","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1146\/annurev.bi.62.070193.003533","article-title":"Transcriptional regulation by cAMP and its receptor protein","volume":"62","author":"Kolb","year":"1993","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.vetmic.2021.109012_bib0080","doi-asserted-by":"crossref","first-page":"25441","DOI":"10.18632\/oncotarget.25192","article-title":"Engineering of a rough auxotrophic mutant Salmonella typhimurium for effective delivery","volume":"9","author":"Lalsiamthara","year":"2018","journal-title":"Oncotarget"},{"key":"10.1016\/j.vetmic.2021.109012_bib0085","doi-asserted-by":"crossref","first-page":"6904","DOI":"10.1016\/j.vaccine.2020.08.062","article-title":"Live attenuated Salmonella enterica serovar Choleraesuis vector delivering a conserved surface protein enolase induces high and broad protection against Streptococcus suis serotypes 2, 7, and 9 in mice","volume":"38","author":"Li","year":"2020","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2021.109012_bib0090","article-title":"Structure and genetics of Escherichia coli O antigens","author":"Liu","year":"2019","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.vetmic.2021.109012_bib0095","article-title":"A farm-to-fork quantitative risk assessment model for SalmonellaHeidelberg resistant to third-generation cephalosporins in broiler chickens in Canada","volume":"330","author":"Lucie Collineau","year":"2020","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.vetmic.2021.109012_bib0100","series-title":"Vaccines Against Invasive Salmonella disease","author":"Maclennan","year":"2014"},{"key":"10.1016\/j.vetmic.2021.109012_bib0105","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1080\/03079457.2017.1348598","article-title":"Evaluation of the effectiveness and safety of a genetically modified live vaccine in broilers challenged with Salmonella heidelberg","volume":"46","author":"Muniz","year":"2017","journal-title":"Avian Pathol."},{"key":"10.1016\/j.vetmic.2021.109012_bib0110","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1637\/9777-051011-Reg.1","article-title":"An investigation on first-week mortality in layers","volume":"56","author":"Olsen","year":"2012","journal-title":"Avian Dis."},{"key":"10.1016\/j.vetmic.2021.109012_bib0115","first-page":"1647","volume":"8","author":"Pei","year":"2017"},{"key":"10.1016\/j.vetmic.2021.109012_bib0120","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.psj.2019.10.038","article-title":"Protection against avian pathogenic Escherichia coli and Salmonella kentucky exhibited in chickens given both probiotics and live Salmonella vaccine","volume":"99","author":"Redweik","year":"2020","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetmic.2021.109012_bib0125","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1586\/14760584.2013.825454","article-title":"Salmonella as a vaccine delivery vehicle","volume":"12","author":"Roland","year":"2013","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.vetmic.2021.109012_bib0130","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1637\/7178-031004R1","article-title":"Expression of Escherichia coli antigens in Salmonella typhimurium as a vaccine to prevent airsacculitis in chickens","volume":"48","author":"Roland","year":"2004","journal-title":"Avian Dis."},{"key":"10.1016\/j.vetmic.2021.109012_bib0135","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2020.103745","volume":"111","author":"Senevirathne","year":"2020","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.vetmic.2021.109012_bib0140","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3389\/fmicb.2015.00028","article-title":"Urinary tract infections attributed to diverse ExPEC strains in food animals: evidence and data gaps","volume":"6","author":"Singer","year":"2015","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109012_bib0145","doi-asserted-by":"crossref","first-page":"7062","DOI":"10.1073\/pnas.1900144116","article-title":"Glycoconjugate vaccine using a genetically modified O antigen induces protective antibodies to Francisella tularensis","volume":"116","author":"Stefanetti","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.vetmic.2021.109012_bib0150","volume":"5","author":"Stromberg","year":"2018"},{"key":"10.1016\/j.vetmic.2021.109012_bib0155","series-title":"Experimental Co-infection of Low Pathogenic Avian Influenza Virus (H9N2) and Escherichia Coli in SPF Broiler Chickens","author":"Taha","year":"2019"},{"key":"10.1016\/j.vetmic.2021.109012_bib0160","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1080\/03079457.2018.1449942","article-title":"Co-infection of turkeys with Escherichia coli (O78) and H6N1 avian influenza virus","volume":"47","author":"Umar","year":"2018","journal-title":"Avian Pathol."},{"key":"10.1016\/j.vetmic.2021.109012_bib0165","series-title":"Glyco-Engineering: Methods and Protocols","first-page":"57","article-title":"Glyco-engineering O-antigen-based vaccines and diagnostics in E. Coli","author":"Vozza","year":"2015"},{"key":"10.1016\/j.vetmic.2021.109012_bib0170","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/1471-2180-11-31","article-title":"Improving Salmonella vector with rec mutation to stabilize the DNA cargoes","volume":"11","author":"Zhang","year":"2011","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.vetmic.2021.109012_bib0175","doi-asserted-by":"crossref","first-page":"5010","DOI":"10.1016\/j.vaccine.2018.07.009","article-title":"Regulated delayed attenuation enhances the immunogenicity and protection provided by recombinant Salmonella enterica serovar Typhimurium vaccines expressing serovar Choleraesuis O-polysaccharides","volume":"36","author":"Zhao","year":"2018","journal-title":"Vaccine"}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113521000353?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113521000353?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T23:14:20Z","timestamp":1759878860000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113521000353"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":35,"alternative-id":["S0378113521000353"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2021.109012","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2021,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Regulated delayed attenuation improves vaccine efficacy in preventing infection from avian pathogenic Escherichia coli O78 and Salmonella typhimurium","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2021.109012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109012"}}