{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T22:21:21Z","timestamp":1793312481630,"version":"4.5.0"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T00:00:00Z","timestamp":1614124800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012245","name":"Science and Technology Planning Project of Guangdong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2021,4]]},"DOI":"10.1016\/j.vetmic.2021.109022","type":"journal-article","created":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T22:02:10Z","timestamp":1614204130000},"page":"109022","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"special_numbering":"C","title":["A new strategy to develop pseudorabies virus-based bivalent vaccine with high immunogenicity of porcine circovirus type 2"],"prefix":"10.1016","volume":"255","author":[{"given":"Xiaofeng","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beili","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qigai","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3440-1369","authenticated-orcid":false,"given":"Xi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7060-5185","authenticated-orcid":false,"given":"Jinxia","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2021.109022_bib0005","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.3201\/eid1911.130177","article-title":"Pseudorabies virus variant in Bartha-K61-vaccinated pigs, China, 2012","volume":"19","author":"An","year":"2013","journal-title":"Emerging Infect. Dis."},{"key":"10.1016\/j.vetmic.2021.109022_bib0010","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.jbiotec.2014.04.006","article-title":"The preparation of porcine circovirus type 2 (PCV2) virus-like particles using a recombinant pseudorabies virus and its application to vaccine development","volume":"181","author":"Chi","year":"2014","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.vetmic.2021.109022_bib0015","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1080\/19420862.2015.1008351","article-title":"Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells","volume":"7","author":"Chng","year":"2015","journal-title":"MAbs"},{"key":"10.1016\/j.vetmic.2021.109022_bib0020","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.1231143","article-title":"Multiplex genome engineering using CRISPR\/Cas systems","volume":"339","author":"Cong","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.vetmic.2021.109022_bib0025","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1006\/viro.1995.1430","article-title":"Herpesvirus of turkey recombinant viruses expressing infectious bursal disease virus (IBDV) VP2 immunogen induce protection against an IBDV virulent challenge in chickens","volume":"211","author":"Darteil","year":"1995","journal-title":"Virology"},{"key":"10.1016\/j.vetmic.2021.109022_bib0030","series-title":"100 Years of Virology","first-page":"87","article-title":"Life beyond eradication: veterinary viruses in basic science","author":"Enquist","year":"1999"},{"key":"10.1016\/j.vetmic.2021.109022_bib0035","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1007\/s11259-007-3469-7","article-title":"Immunogenicity of empty capsids of porcine circovius type 2 produced in insect cells","volume":"31","author":"Fan","year":"2007","journal-title":"Vet. Res. Commun."},{"issue":"6","key":"10.1016\/j.vetmic.2021.109022_bib0040","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1111\/j.1939-1676.2009.0389.x","article-title":"Porcine circovirus type 2 and porcine circovirus-associated disease","volume":"23","author":"Gillespie","year":"2009","journal-title":"J. Vet. Intern. Med."},{"key":"10.1016\/j.vetmic.2021.109022_bib0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112113","article-title":"Capillary-assisted microfluidic biosensing platform captures single cell secretion dynamics in nanoliter compartments","volume":"155","author":"Hassanzadeh-Barforoushi","year":"2020","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.vetmic.2021.109022_bib0050","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/j.vaccine.2014.12.028","article-title":"Vaccine protection of chickens against antigenically diverse H5 highly pathogenic avian influenza isolates with a live HVT vector vaccine expressing the influenza hemagglutinin gene derived from a clade 2.2 avian influenza virus","volume":"33","author":"Kapczynski","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2021.109022_bib0055","doi-asserted-by":"crossref","first-page":"2518","DOI":"10.1074\/mcp.TIR118.000914","article-title":"Chromobodies to quantify changes of endogenous protein concentration in living cells","volume":"17","author":"Keller","year":"2018","journal-title":"Mol. Cell Proteomics"},{"key":"10.1016\/j.vetmic.2021.109022_bib0060","article-title":"High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice","volume":"6","author":"Kim","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.vetmic.2021.109022_bib0065","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.2006660","article-title":"Direct visualization of single-molecule membrane protein interactions in living cells","volume":"16","author":"Kim","year":"2018","journal-title":"PLoS Biol."},{"key":"10.1016\/j.vetmic.2021.109022_bib0070","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1038\/s41592-019-0541-5","article-title":"FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics","volume":"16","author":"Kinjo","year":"2019","journal-title":"Nat. Methods"},{"key":"10.1016\/j.vetmic.2021.109022_bib0075","doi-asserted-by":"crossref","first-page":"8257","DOI":"10.1016\/j.vaccine.2011.08.115","article-title":"Recombinant herpesvirus of turkeys as a vector-based vaccine against highly pathogenic H7N1 avian influenza and Marek\u2019s disease","volume":"29","author":"Li","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2021.109022_bib0080","doi-asserted-by":"crossref","first-page":"19176","DOI":"10.1038\/srep19176","article-title":"A CRISPR\/Cas9 and Cre\/Lox system-based express vaccine development strategy against re-emerging pseudorabies virus","volume":"6","author":"Liang","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.vetmic.2021.109022_bib0085","doi-asserted-by":"crossref","first-page":"2634","DOI":"10.1016\/j.celrep.2015.11.048","article-title":"Quantification of protein levels in single living cells","volume":"13","author":"Lo","year":"2015","journal-title":"Cell Rep."},{"key":"10.1016\/j.vetmic.2021.109022_bib0090","series-title":"Fenner\u2019s Veterinary Virology","first-page":"237","article-title":"Chapter 13. circoviridae","author":"MacLachlan","year":"2011"},{"key":"10.1016\/j.vetmic.2021.109022_bib0095","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1746-6148-2-6","article-title":"Correlation between the presence of neutralizing antibodies against porcine circovirus 2 (PCV2) and protection against replication of the virus and development of PCV2-associated disease","volume":"2","author":"Meerts","year":"2006","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.vetmic.2021.109022_bib0100","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/0147-9571(91)90128-Z","article-title":"Molecular biology of pseudorabies (Aujeszky\u2019s disease) virus","volume":"14","author":"Mettenleiter","year":"1991","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.vetmic.2021.109022_bib0105","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.vetmic.2019.03.002","article-title":"Evaluation of a porcine circovirus type 2a (PCV2a) vaccine efficacy against experimental PCV2a, PCV2b, and PCV2d challenge","volume":"231","author":"Park","year":"2019","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109022_bib0110","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3390\/vaccines8010115","article-title":"Bovine Herpesvirus-4-Vectored Delivery of Nipah Virus Glycoproteins Enhances T Cell Immunogenicity in Pigs","volume":"8","author":"Pedrera","year":"2020","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.vetmic.2021.109022_bib0115","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1128\/MMBR.69.3.462-500.2005","article-title":"Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine","volume":"69","author":"Pomeranz","year":"2005","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.vetmic.2021.109022_bib0120","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1586\/14760584.2015.983084","article-title":"Best practice and future challenges for vaccination against porcine circovirus type 2","volume":"14","author":"Segales","year":"2015","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.vetmic.2021.109022_bib0125","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.vetmic.2006.08.026","article-title":"Generation and immunogenicity of a recombinant pseudorabies virus expressing cap protein of porcine circovirus type 2","volume":"119","author":"Song","year":"2007","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109022_bib0130","doi-asserted-by":"crossref","first-page":"4293","DOI":"10.1096\/fj.201701129R","article-title":"CRISPR\/Cas9\u2010mediated 2\u2010sgRNA cleavage facilitates Pseudorabies virus editing","volume":"32","author":"Tang","year":"2018","journal-title":"Faseb J."},{"key":"10.1016\/j.vetmic.2021.109022_bib0135","doi-asserted-by":"crossref","first-page":"7902","DOI":"10.1073\/pnas.1019507108","article-title":"Site-specific integrase-mediated transgenesis in mice via pronuclear injection","volume":"108","author":"Tasic","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.vetmic.2021.109022_bib0140","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1099\/vir.0.011940-0","article-title":"Glycoprotein G from pseudorabies virus binds to chemokines with high affinity and inhibits their function","volume":"91","author":"Viejo-Borbolla","year":"2010","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.vetmic.2021.109022_bib0145","doi-asserted-by":"crossref","first-page":"16273","DOI":"10.1038\/srep16273","article-title":"2A self-cleaving peptide-based multi-gene expression system in the silkworm Bombyx mori","volume":"5","author":"Wang","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.vetmic.2021.109022_bib0150","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1007\/s00253-012-4015-2","article-title":"Characterization of porcine circovirus type 2 (PCV2) capsid particle assembly and its application to virus-like particle vaccine development","volume":"95","author":"Wu","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.vetmic.2021.109022_bib0155","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.virusres.2015.02.010","article-title":"Construction and immunogenicity of a recombinant pseudorabies virus co-expressing porcine circovirus type 2 capsid protein and interleukin 18","volume":"201","author":"Zheng","year":"2015","journal-title":"Virus Res."},{"key":"10.1016\/j.vetmic.2021.109022_bib0160","doi-asserted-by":"crossref","first-page":"e34","DOI":"10.1093\/nar\/gkt1290","article-title":"DICE, an efficient system for iterative genomic editing in human pluripotent stem cells","volume":"42","author":"Zhu","year":"2014","journal-title":"Nucleic Acids Res."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113521000456?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113521000456?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T23:12:56Z","timestamp":1759878776000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113521000456"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4]]},"references-count":32,"alternative-id":["S0378113521000456"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2021.109022","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2021,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A new strategy to develop pseudorabies virus-based bivalent vaccine with high immunogenicity of porcine circovirus type 2","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2021.109022","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"109022"}}