{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T20:14:18Z","timestamp":1768767258562,"version":"3.49.0"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2016YFD0500103"],"award-info":[{"award-number":["2016YFD0500103"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31672569"],"award-info":[{"award-number":["31672569"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["318607804"],"award-info":[{"award-number":["318607804"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2021,6]]},"DOI":"10.1016\/j.vetmic.2021.109074","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T12:33:59Z","timestamp":1619440439000},"page":"109074","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["ATPase and helicase activities of porcine epidemic diarrhea virus nsp13"],"prefix":"10.1016","volume":"257","author":[{"given":"Jie","family":"Ren","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Puxian","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaobo","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1406-6409","authenticated-orcid":false,"given":"Liurong","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2021.109074_bib0005","doi-asserted-by":"crossref","first-page":"e00235","DOI":"10.1128\/mSphere.00235-16","article-title":"Biochemical characterization of middle east respiratory syndrome coronavirus helicase","volume":"1","author":"Adedeji","year":"2016","journal-title":"mSphere"},{"key":"10.1016\/j.vetmic.2021.109074_bib0010","doi-asserted-by":"crossref","first-page":"10910","DOI":"10.1128\/JVI.01919-12","article-title":"Complete genome sequence of porcine epidemic diarrhea virus strain AJ1102 isolated from a suckling piglet with acute diarrhea in China","volume":"86","author":"Bi","year":"2012","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2021.109074_bib0015","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.molcel.2007.07.014","article-title":"The long unwinding road of RNA helicases","volume":"27","author":"Bleichert","year":"2007","journal-title":"Mol. Cell"},{"key":"10.1016\/j.vetmic.2021.109074_bib0020","first-page":"629","article-title":"Nidovirales: a new order comprising Coronaviridae and Arteriviridae","volume":"142","author":"Cavanagh","year":"1997","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetmic.2021.109074_bib0025","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1016\/j.cell.2020.07.033","article-title":"Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex","volume":"182","author":"Chen","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.vetmic.2021.109074_bib0030","first-page":"30","article-title":"Viral disease affects U.S. pigs: porcine epidemic diarrhea found in at least 11 states","volume":"243","author":"Cima","year":"2013","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"10.1016\/j.vetmic.2021.109074_bib0035","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1177\/030098588201900108","article-title":"Pathology of experimental CV777 coronavirus enteritis in piglets. I. Histological and histochemical study","volume":"19","author":"Coussement","year":"1982","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.vetmic.2021.109074_bib0040","doi-asserted-by":"crossref","first-page":"5619","DOI":"10.1128\/JVI.78.11.5619-5632.2004","article-title":"Multiple enzymatic activities associated with severe acute respiratory syndrome coronavirus helicase","volume":"78","author":"Ivanov","year":"2004","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2021.109074_bib0045","doi-asserted-by":"crossref","first-page":"6538","DOI":"10.1093\/nar\/gkz409","article-title":"Delicate structural coordination of the Severe Acute Respiratory Syndrome coronavirus Nsp13 upon ATP hydrolysis","volume":"47","author":"Jia","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.vetmic.2021.109074_bib0050","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.virusres.2017.05.028","article-title":"Cellular DEAD-box RNA helicase 18 (DDX18) promotes the PRRSV Replication via Interaction with Virus nsp2 and nsp10","volume":"238","author":"Jin","year":"2017","journal-title":"Virus Res."},{"key":"10.1016\/j.vetmic.2021.109074_bib0055","doi-asserted-by":"crossref","first-page":"2583","DOI":"10.1128\/jvi.71.4.2583-2590.1997","article-title":"Virus-encoded RNA helicases","volume":"71","author":"Kadar\u00e9","year":"1997","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2021.109074_bib0060","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1023\/A:1011831902219","article-title":"Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence","volume":"23","author":"Kocherhans","year":"2001","journal-title":"Virus Genes"},{"key":"10.1016\/j.vetmic.2021.109074_bib0065","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.virusres.2014.12.001","article-title":"What we know but do not understand about nidovirus helicases","volume":"202","author":"Lehmann","year":"2015","journal-title":"Virus Res."},{"key":"10.1016\/j.vetmic.2021.109074_bib0070","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/j.1365-2958.1992.tb00831.x","article-title":"Escherichia coli DNA helicases: mechanisms of DNA unwinding","volume":"6","author":"Lohman","year":"1992","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109074_bib0075","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1136\/vr.162.10.307","article-title":"Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy","volume":"162","author":"Martelli","year":"2008","journal-title":"Vet. Rec."},{"key":"10.1016\/j.vetmic.2021.109074_bib0080","doi-asserted-by":"crossref","first-page":"632","DOI":"10.4161\/rna.7.6.14467","article-title":"RNA remodeling by chaperones and helicases","volume":"7","author":"Musier-Forsyth","year":"2010","journal-title":"RNA Biol."},{"key":"10.1016\/j.vetmic.2021.109074_bib0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.jviromet.2019.113772","article-title":"Rapid manipulation of the porcine epidemic diarrhea virus genome by CRISPR\/Cas9 technology","volume":"276","author":"Peng","year":"2020","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.vetmic.2021.109074_bib0090","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/BF01317606","article-title":"A new coronavirus-like particle associated with diarrhea in swine","volume":"58","author":"Pensaert","year":"1978","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetmic.2021.109074_bib0095","doi-asserted-by":"crossref","first-page":"5111","DOI":"10.2527\/jas.2015-9136","article-title":"Assessment of the economic impacts of porcine epidemic diarrhea virus in the United States","volume":"93","author":"Schulz","year":"2015","journal-title":"J. Anim. Sci."},{"key":"10.1016\/j.vetmic.2021.109074_bib0100","doi-asserted-by":"crossref","first-page":"326","DOI":"10.3390\/v9110326","article-title":"Helicase Domain of West Nile Virus NS3 Protein Plays a Role in Inhibition of Type I Interferon Signalling","volume":"9","author":"Setoh","year":"2017","journal-title":"Viruses"},{"key":"10.1016\/j.vetmic.2021.109074_bib0105","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s12250-020-00242-1","article-title":"SARS-Coronavirus-2 Nsp13 Possesses NTPase and RNA Helicase Activities That Can Be Inhibited by Bismuth Salts","volume":"35","author":"Shu","year":"2020","journal-title":"Virol. Sin."},{"key":"10.1016\/j.vetmic.2021.109074_bib0110","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1146\/annurev.biochem.76.052305.115300","article-title":"Structure and mechanism of helicases and nucleic acid translocases","volume":"76","author":"Singleton","year":"2007","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.vetmic.2021.109074_bib0115","doi-asserted-by":"crossref","first-page":"39578","DOI":"10.1074\/jbc.C300328200","article-title":"The severe acute respiratory syndrome (SARS) coronavirus NTPase\/helicase belongs to a distinct class of 5\u2019 to 3\u2019 viral helicases","volume":"278","author":"Tanner","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetmic.2021.109074_bib0120","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000054","article-title":"SARS-coronavirus replication\/transcription complexes are membrane-protected and need a host factor for activity in vitro","volume":"4","author":"van Hemert","year":"2008","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.vetmic.2021.109074_bib0125","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1002\/j.1460-2075.1982.tb01276.x","article-title":"Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold","volume":"1","author":"Walker","year":"1982","journal-title":"EMBO J."},{"key":"10.1016\/j.vetmic.2021.109074_bib0130","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005067","article-title":"Human enterovirus nonstructural protein 2CATPase functions as both an RNA helicase and ATP-Independent RNA chaperone","volume":"11","author":"Xia","year":"2015","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.vetmic.2021.109074_bib0135","doi-asserted-by":"crossref","first-page":"39546","DOI":"10.1074\/jbc.M111.242206","article-title":"Coronavirus infection induces DNA replication stress partly through interaction of its nonstructural protein 13 with the p125 subunit of DNA polymerase \u03b4","volume":"286","author":"Xu","year":"2011","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetmic.2021.109074_bib0140","doi-asserted-by":"crossref","first-page":"2538","DOI":"10.1093\/nar\/gkt1256","article-title":"A cypovirus VP5 displays the RNA chaperone-like activity that destabilizes RNA helices and accelerates strand annealing","volume":"42","author":"Yang","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.vetmic.2021.109074_bib0145","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1080\/22221751.2020.1780953","article-title":"SARS-CoV-2 nsp13, nsp14, nsp15 and orf6 function as potent interferon antagonists","volume":"9","author":"Yuen","year":"2020","journal-title":"Emerg Microbes Infect."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113521000973?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113521000973?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T23:14:02Z","timestamp":1759878842000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113521000973"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6]]},"references-count":29,"alternative-id":["S0378113521000973"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2021.109074","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2021,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"ATPase and helicase activities of porcine epidemic diarrhea virus nsp13","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2021.109074","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109074"}}