{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T01:34:29Z","timestamp":1786325669916,"version":"build-2736575974"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002551","name":"Seoul National University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002551","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2021,7]]},"DOI":"10.1016\/j.vetmic.2021.109100","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T12:10:06Z","timestamp":1620130206000},"page":"109100","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Experimental efficacy of a trivalent vaccine containing porcine circovirus types 2a\/b (PCV2a\/b) and Mycoplasma hyopneumoniae against PCV2d and M. hyopneumoniae challenges"],"prefix":"10.1016","volume":"258","author":[{"given":"Siyeon","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taehwan","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kee Hwan","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyejean","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeongmin","family":"Suh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5252-3266","authenticated-orcid":false,"given":"Chanhee","family":"Chae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2021.109100_bib0005","doi-asserted-by":"crossref","first-page":"669","DOI":"10.2460\/javma.2001.218.669","article-title":"2000 Report of the AVMA panel on euthanasia","volume":"218","author":"Beaver","year":"2001","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"10.1016\/j.vetmic.2021.109100_bib0010","first-page":"761","article-title":"Porcine circovirus-2 associated disease in swine in Ontario (2004 to 2005)","volume":"47","author":"Carman","year":"2006","journal-title":"Can. Vet. J."},{"key":"10.1016\/j.vetmic.2021.109100_bib0015","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S1090-0233(03)00182-5","article-title":"Postweaning multisystemic wasting syndrome: a review of etiology, diagnosis and pathology","volume":"168","author":"Chae","year":"2004","journal-title":"Vet. J."},{"key":"10.1016\/j.vetmic.2021.109100_bib0020","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.tvjl.2004.01.012","article-title":"A review of porcine circovirus 2-associated syndromes and diseases","volume":"169","author":"Chae","year":"2005","journal-title":"Vet. J."},{"key":"10.1016\/j.vetmic.2021.109100_bib0025","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.tvjl.2009.12.023","article-title":"Genotypic shift of porcine circovirus type 2 from PCV-2a to PCV-2b in Spain from 1985 to 2008","volume":"187","author":"Cortey","year":"2011","journal-title":"Vet. J."},{"key":"10.1016\/j.vetmic.2021.109100_bib0030","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1111\/j.1751-0813.1997.tb14383.x","article-title":"Serum and mucosal antibody responses and protection in pigs vaccinated against Mycoplasma hyopneumoniae with vaccines containing a denatured membrane antigen pool and adjuvant","volume":"75","author":"Djordjevic","year":"1997","journal-title":"Aust. Vet. J."},{"key":"10.1016\/j.vetmic.2021.109100_bib0035","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.vetmic.2004.05.007","article-title":"Development of two real-time PCR assays for the detection of Mycoplasma hyopneumoniae in clinical samples","volume":"102","author":"Dubosson","year":"2004","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0040","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1080\/00401706.1964.10490181","article-title":"Multiple comparisons using rank sums","volume":"6","author":"Dunn","year":"1964","journal-title":"Technometrics"},{"key":"10.1016\/j.vetmic.2021.109100_bib0045","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.vaccine.2007.12.019","article-title":"Porcine circovirus type 2 (PCV2) vaccination of conventional pigs prevents viremia against PCV2 isolates of different genotypes and geographic origins","volume":"26","author":"Fort","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2021.109100_bib0050","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.vetimm.2012.09.001","article-title":"Evaluation of cell-mediated immune responses against porcine circovirus type 2 (PCV2) Cap and Rep proteins after vaccination with a commercial PCV2 sub-unit vaccine","volume":"150","author":"Fort","year":"2012","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0055","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0208585","article-title":"Porcine circovirus 2 (PCV-2) genotype update and proposal of a new genotyping methodology","volume":"13","author":"Franzo","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.vetmic.2021.109100_bib0060","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.ympev.2016.04.028","article-title":"Phylodynamic analysis of porcine circovirus type 2 reveals global waves of emerging genotypes and the circulation of recombinant forms","volume":"100","author":"Franzo","year":"2016","journal-title":"Mol. Phylogenet. Evol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0065","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1111\/j.1939-1676.2009.0389.x","article-title":"Porcine circovirus type 2 and porcine circovirus-associated disease","volume":"23","author":"Gillespie","year":"2009","journal-title":"J. Vet. Intern. Med."},{"key":"10.1016\/j.vetmic.2021.109100_bib0070","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1177\/030098589503200606","article-title":"Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus","volume":"32","author":"Halbur","year":"1995","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0075","doi-asserted-by":"crossref","first-page":"455","DOI":"10.3389\/fmicb.2018.00455","article-title":"Evaluation of the efficacy and cross-protective immunity of live-attenuated chimeri PCV1-2b vaccine against PCV2b and PCV2d subtype challenge in pigs","volume":"9","author":"Huan","year":"2018","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0080","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.vetmic.2015.02.027","article-title":"Comparison of three commercial one-dose porcine circovirus type 2 (PCV2) vaccines in a herd with concurrent circulation of PCV2b and mutant PCV2b","volume":"177","author":"Jeong","year":"2015","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0085","first-page":"39","article-title":"Comparison of 3 vaccination strategies against porcine reproductive and respiratory syndrome virus, Mycoplasma hyopneumoniae, and porcine circovirus type 2 on 3 pathogen challenge model","volume":"82","author":"Jeong","year":"2018","journal-title":"Can. J. Vet. Res."},{"key":"10.1016\/j.vetmic.2021.109100_bib0090","doi-asserted-by":"crossref","first-page":"99","DOI":"10.3390\/v9050099","article-title":"Porcine circovirus type 2 (PCV2) vaccines in the context of current molecular epidemiology","volume":"9","author":"Karuppannan","year":"2017","journal-title":"Viruses"},{"key":"10.1016\/j.vetmic.2021.109100_bib0095","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jcpa.2004.02.001","article-title":"Expression of monocyte chemoattractant protein-1 and macrophage inflammatory protein-1 in porcine circovirus 2-induced granulomatous inflammation","volume":"131","author":"Kim","year":"2004","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0100","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1053\/jcpa.2002.0605","article-title":"Pathogenesis of postweaning multisystemic wasting syndrome reproduced by co-infection with Korean isolates of porcine circovirus 2 and porcine parvovirus","volume":"128","author":"Kim","year":"2003","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0105","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.virusres.2016.11.015","article-title":"Genotypic diversity of porcine circovirus type 2 (PCV2) and genotype shift to PCV2d in Korean pig population","volume":"228","author":"Kwon","year":"2017","journal-title":"Virus Res."},{"key":"10.1016\/j.vetmic.2021.109100_bib0110","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1080\/01652176.1996.9694628","article-title":"Enzootic pneumonia in pigs","volume":"18","author":"Maes","year":"1996","journal-title":"Vet. Q."},{"key":"10.1016\/j.vetmic.2021.109100_bib0115","doi-asserted-by":"crossref","first-page":"4689","DOI":"10.1016\/j.vaccine.2014.05.072","article-title":"Immune responses of a chimeric protein vaccine containing Mycoplasma hyopneumoniae antigens and LTB against experimental M. Hyopneumoniae infection in pigs","volume":"32","author":"Marchioro","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2021.109100_bib0120","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.vetmic.2010.12.008","article-title":"One dose of a porcine circovirus 2 subunit vaccine induces humoral and cell-mediated immunity and protects against porcine circovirus-associated disease under field conditions","volume":"149","author":"Martelli","year":"2011","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0125","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1186\/s12917-017-1195-0","article-title":"Efficacy of one dose vaccinaion against experimental infection with two Mycoplasma hyopneumoniae strains","volume":"13","author":"Michiels","year":"2017","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.vetmic.2021.109100_bib0130","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1354\/vp.41-6-624","article-title":"Experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with Mycoplasma hyopneumoniae and porcine circovirus type 2","volume":"41","author":"Opriessnig","year":"2004","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0135","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.vetmic.2012.12.019","article-title":"Emergence of a novel mutant PCV2b variant associated with clinical PCVAD in two vaccinated pig farms in the US concurrently infected with PPV2","volume":"163","author":"Opriessnig","year":"2013","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0140","doi-asserted-by":"crossref","first-page":"4342","DOI":"10.1016\/j.vaccine.2014.06.004","article-title":"Commercial PCV2a-based vaccines are effective in protecting naturally PCV2b-infected finisher pigs against experimental challenge with a 2012 mutant PCV2","volume":"32","author":"Opriessnig","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2021.109100_bib0145","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.vaccine.2013.11.010","article-title":"A commercial vaccine based on PCV2a and an experimental vaccine based on a variant mPCV2b are both effective in protecting pigs against challenge with a 2013 U.S. Variant mPCV2b strain","volume":"32","author":"Opriessnig","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2021.109100_bib0150","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.vaccine.2016.11.085","article-title":"A commercial porcine circovirus (PCV) type 2a-based vaccine reduces PCV2d viremia and shedding and prevents PCV2d transmission to na\u00efve pigs under experimental conditions","volume":"35","author":"Opriessnig","year":"2017","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2021.109100_bib0155","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1128\/CVI.00497-12","article-title":"Comparative effects of vaccination against porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) in a PCV2-PRRSV challenge model","volume":"20","author":"Park","year":"2013","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0160","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.vetmic.2019.03.002","article-title":"Evaluation of a porcine circovirus type 2a (PCV2a) vaccine efficacy against experimental PCV2a, PCV2b, and PCV2d challenge","volume":"231","author":"Park","year":"2019","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0165","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.vetmic.2017.02.014","article-title":"Comparison of sample types and disgnostic methods for in vivo detetion of Mycoplasma hyopneumoniae during early stage of infection","volume":"203","author":"Pieters","year":"2017","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0170","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1089\/vim.2000.13.143","article-title":"Characterization of immune response of young pigs to porcine circovirus type 2 infection","volume":"13","author":"Pogranichnyy","year":"2000","journal-title":"Viral Immunol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0175","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.meegid.2015.05.023","article-title":"First identification of porcine circovirus type 2b mutant in pigs from Uruguay","volume":"33","author":"Ramos","year":"2015","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0180","doi-asserted-by":"crossref","first-page":"6671","DOI":"10.1016\/j.vaccine.2012.08.065","article-title":"Efficacy of a reformulated inactivated chimeric PCV1-2 vaccine based on clinical, virological, pathological and immunological examination under field conditions","volume":"30","author":"Seo","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2021.109100_bib0185","doi-asserted-by":"crossref","first-page":"3107","DOI":"10.1007\/s00705-014-2164-6","article-title":"Genetic and antigenic characterization of a newly emerging porcine circovirus type 2b mutant first isolated in cases of vaccine failure in Korea","volume":"159","author":"Seo","year":"2014","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0190","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.2460\/ajvr.2000.61.1384","article-title":"Evaluation of local and systemic immune responses induced by intramuscular injection of a Mycoplasma hyopneumoniae bacterin to pigs","volume":"61","author":"Thacker","year":"2000","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.vetmic.2021.109100_bib0195","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s11262-008-0217-1","article-title":"Phylogenetic analysis of porcine circovirus type 2 (PCV2) pre- and post-epizootic postweaning multisystemic wasting syndrome (PMWS)","volume":"36","author":"Timmusk","year":"2008","journal-title":"Virus Genes"},{"key":"10.1016\/j.vetmic.2021.109100_bib0200","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1099\/0022-1317-49-7-613","article-title":"A potential role for tumour necrosis factor-\u03b1 in synergy between porcine respiratory coronavirus and bacterial lipopolysaccharide in the induction of respiratory disease in pigs","volume":"49","author":"Van Reeth","year":"2000","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0205","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.vetmic.2008.10.028","article-title":"A new emerging genotype subgroup within PCV-2b dominates the PMWS epizooty in Switzerland","volume":"136","author":"Wiederkehr","year":"2009","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2021.109100_bib0210","doi-asserted-by":"crossref","first-page":"1830","DOI":"10.1099\/vir.0.000100","article-title":"Global molecular genetic analysis of porcine circovirus type 2 (PCV2) sequences confirms the presence of four main PCV2 genotypes and reveals a rapid increase of PCV2d","volume":"96","author":"Xiao","year":"2015","journal-title":"J. Gen. Virol."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113521001231?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113521001231?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T23:12:44Z","timestamp":1759878764000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113521001231"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7]]},"references-count":42,"alternative-id":["S0378113521001231"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2021.109100","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2021,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Experimental efficacy of a trivalent vaccine containing porcine circovirus types 2a\/b (PCV2a\/b) and Mycoplasma hyopneumoniae against PCV2d and M. hyopneumoniae challenges","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2021.109100","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109100"}}