{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T15:03:29Z","timestamp":1794409409690,"version":"4.9.0"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T00:00:00Z","timestamp":1679529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014219","name":"National Science Fund for Distinguished Young Scholars","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016698","name":"Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province","doi-asserted-by":"publisher","award":["32102652"],"award-info":[{"award-number":["32102652"]}],"id":[{"id":"10.13039\/100016698","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016698","name":"Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province","doi-asserted-by":"publisher","award":["32125037"],"award-info":[{"award-number":["32125037"]}],"id":[{"id":"10.13039\/100016698","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005153","name":"China National Funds for Distinguished Young Scientists","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005153","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.vetmic.2023.109728","type":"journal-article","created":{"date-parts":[[2023,3,21]],"date-time":"2023-03-21T05:04:07Z","timestamp":1679375047000},"page":"109728","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"special_numbering":"C","title":["Safety and immunogenicity of a TK\/ gI\/gE gene-deleted feline herpesvirus-1 mutant constructed via CRISPR\/Cas9 in feline"],"prefix":"10.1016","volume":"281","author":[{"given":"Mengfang","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzhou","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisha","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoshuai","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengxia","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuejun","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiqing","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2023.109728_bib1","doi-asserted-by":"crossref","first-page":"8933","DOI":"10.1128\/JVI.72.11.8933-8942.1998","article-title":"The herpes simplex virus gE-gI complex facilitates cell-to-cell spread and binds to components of cell junctions","volume":"72","author":"Dingwell","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2023.109728_bib2","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1017\/S0022172400025109","article-title":"The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice","volume":"81","author":"Field","year":"1978","journal-title":"J. Hyg. (Lond.)"},{"key":"10.1016\/j.vetmic.2023.109728_bib3","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1051\/vetres:2006063","article-title":"Feline herpesvirus","volume":"38","author":"Gaskell","year":"2007","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetmic.2023.109728_bib4","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1016\/j.vaccine.2021.01.026","article-title":"Evaluation of safety and immunogenicity of feline vaccines with reduced volume","volume":"39","author":"Jas","year":"2021","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2023.109728_bib5","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/0378-1135(95)00137-9","article-title":"Virulence and immunogenicity in calves of thymidine kinase- and glycoprotein E-negative bovine herpesvirus 1 mutants","volume":"48","author":"Kaashoek","year":"1996","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2023.109728_bib6","doi-asserted-by":"crossref","first-page":"9431","DOI":"10.1128\/JVI.01317-13","article-title":"Glycoproteins gE and gI are required for efficient KIF1A-dependent anterograde axonal transport of alphaherpesvirus particles in neurons","volume":"87","author":"Kratchmarov","year":"2013","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2023.109728_bib7","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1006\/viro.1996.0318","article-title":"Glycoproteins gl and gE of feline herpesvirus-1 are virulence genes: safety and efficacy of a gl-gE deletion mutant in the natural host","volume":"220","author":"Kruger","year":"1996","journal-title":"Virology"},{"key":"10.1016\/j.vetmic.2023.109728_bib8","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.jfms.2005.12.001","article-title":"Effects of a single dose of an intranasal feline herpesvirus 1, calicivirus, and panleukopenia vaccine on clinical signs and virus shedding after challenge with virulent feline herpesvirus 1","volume":"8","author":"Lappin","year":"2006","journal-title":"J. Feline Med. Surg."},{"key":"10.1016\/j.vetmic.2023.109728_bib9","first-page":"13","article-title":"Safety and efficacy of felid herpesvirus-1 deletion mutants in cats","author":"Lee","year":"2021","journal-title":"Viruses"},{"key":"10.1016\/j.vetmic.2023.109728_bib10","first-page":"12","article-title":"Comparison of gE\/gI- and TK\/gE\/gI-gene-deleted pseudorabies virus vaccines mediated by CRISPR\/Cas9 and Cre\/Lox systems","author":"Li","year":"2020","journal-title":"Viruses"},{"key":"10.1016\/j.vetmic.2023.109728_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2021.109104","article-title":"Pathogenicity and immunogenicity of a gI\/gE\/TK\/UL13-gene-deleted variant pseudorabies virus strain in swine","volume":"258","author":"Lv","year":"2021","journal-title":"Vet. Microbiol"},{"key":"10.1016\/j.vetmic.2023.109728_bib12","doi-asserted-by":"crossref","DOI":"10.5402\/2012\/495830","article-title":"Felid herpesvirus type 1 infection in cats: a natural host model for alphaherpesvirus pathogenesis","volume":"2012","author":"Maes","year":"2012","journal-title":"ISRN Vet. Sci."},{"key":"10.1016\/j.vetmic.2023.109728_bib13","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1053\/j.ctsap.2004.12.013","article-title":"Update on pathogenesis, diagnosis, and treatment of feline herpesvirus type 1","volume":"20","author":"Maggs","year":"2005","journal-title":"Clin. Tech. Small Anim. Pr."},{"key":"10.1016\/j.vetmic.2023.109728_bib14","doi-asserted-by":"crossref","first-page":"5276","DOI":"10.1016\/j.vaccine.2013.08.052","article-title":"New attenuated vaccine against columnaris disease in fish: choosing the right parental strain is critical for vaccine efficacy","volume":"31","author":"Mohammed","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2023.109728_bib15","doi-asserted-by":"crossref","first-page":"94","DOI":"10.2460\/javma.231.1.94","article-title":"Adverse events after vaccine administration in cats: 2,560 cases (2002-2005)","volume":"231","author":"Moore","year":"2007","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"10.1016\/j.vetmic.2023.109728_bib16","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/0147-9571(79)90023-7","article-title":"A review of feline viral rhinotracheitis (feline herpesvirus I infection)","volume":"2","author":"Povey","year":"1979","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.vetmic.2023.109728_bib17","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1111\/1348-0421.12180","article-title":"Engineering large viral DNA genomes using the CRISPR-Cas9 system","volume":"58","author":"Suenaga","year":"2014","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.vetmic.2023.109728_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2022.109387","article-title":"Effective cross-protection of a lyophilized live gE\/gI\/TK-deleted pseudorabies virus (PRV) vaccine against classical and variant PRV challenges","volume":"267","author":"Sun","year":"2022","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2023.109728_bib19","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1006\/viro.1995.9959","article-title":"A feline herpesvirus-1 recombinant with a deletion in the genes for glycoproteins gI and gE is effective as a vaccine for feline rhinotracheitis","volume":"214","author":"Sussman","year":"1995","journal-title":"Virology"},{"key":"10.1016\/j.vetmic.2023.109728_bib20","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.virol.2010.02.021","article-title":"Complete genomic sequence and an infectious BAC clone of feline herpesvirus-1 (FHV-1)","volume":"401","author":"Tai","year":"2010","journal-title":"Virology"},{"key":"10.1016\/j.vetmic.2023.109728_bib21","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.virusres.2016.05.001","article-title":"In vitro characterization of felid herpesvirus 1 (FHV-1) mutants generated by recombineering in a recombinant BAC vector","volume":"221","author":"Tai","year":"2016","journal-title":"Virus Res."},{"key":"10.1016\/j.vetmic.2023.109728_bib22","first-page":"92","article-title":"Feline Herpesvirus 1 US3 blocks the type i interferon signal pathway by targeting interferon regulatory factor 3 dimerization in a kinase-independent manner","author":"Tian","year":"2018","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2023.109728_bib23","first-page":"71","article-title":"Equine Herpesvirus type 1 mutant defective in glycoprotein E gene as candidate","author":"TSUJIMURA 1","year":"2009","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.vetmic.2023.109728_bib24","first-page":"95","article-title":"CRISPR\/Cas9-constructed pseudorabies virus mutants reveal the importance of UL13 in Alphaherpesvirus escape from genome silencing","author":"Van Cleemput","year":"2021","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2023.109728_bib25","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00745-21","article-title":"Establishment of full-length cDNA clones and an efficient oral infection model for feline coronavirus in cats","volume":"95","author":"Wang","year":"2021","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2023.109728_bib26","doi-asserted-by":"crossref","first-page":"2389","DOI":"10.1007\/s007050050250","article-title":"Effect of a live attenuated intranasal vaccine on latency and shedding of feline herpesvirus 1 in domestic cats","volume":"142","author":"Weigler","year":"1997","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetmic.2023.109728_bib27","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1007\/s10529-015-1796-2","article-title":"A simple and rapid approach to manipulate pseudorabies virus genome by CRISPR\/Cas9 system","volume":"37","author":"Xu","year":"2015","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.vetmic.2023.109728_bib28","first-page":"13","article-title":"Recombinant Pseudorabies virus with TK\/gE gene deletion and Flt3L Co-expression enhances the innate and adaptive immune response via activating dendritic cells","author":"Yao","year":"2021","journal-title":"Viruses"},{"key":"10.1016\/j.vetmic.2023.109728_bib29","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1292\/jvms.57.709","article-title":"Construction of the recombinant feline herpesvirus type 1 deleted thymidine kinase gene","volume":"57","author":"Yokoyama","year":"1995","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.vetmic.2023.109728_bib30","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1007\/BF01718312","article-title":"Pathogenicity and vaccine efficacy of a thymidine kinase-deficient mutant of feline herpesvirus type 1 in cats","volume":"141","author":"Yokoyama","year":"1996","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetmic.2023.109728_bib31","doi-asserted-by":"crossref","first-page":"4622","DOI":"10.1128\/jvi.62.12.4622-4626.1988","article-title":"Complex between glycoproteins gI and gp63 of pseudorabies virus: its effect on virus replication","volume":"62","author":"Zuckermann","year":"1988","journal-title":"J. Virol."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113523000809?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113523000809?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T23:20:28Z","timestamp":1759879228000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113523000809"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":31,"alternative-id":["S0378113523000809"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2023.109728","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Safety and immunogenicity of a TK\/ gI\/gE gene-deleted feline herpesvirus-1 mutant constructed via CRISPR\/Cas9 in feline","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2023.109728","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"109728"}}