{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T20:26:37Z","timestamp":1771273597664,"version":"3.50.1"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2023,10]]},"DOI":"10.1016\/j.vetmic.2023.109847","type":"journal-article","created":{"date-parts":[[2023,8,12]],"date-time":"2023-08-12T18:38:03Z","timestamp":1691865483000},"page":"109847","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Efficacy of a porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) natural recombinant against a heterologous PRRSV-1 isolate both clustered within the subgroup of BJEU06-1-like isolates"],"prefix":"10.1016","volume":"285","author":[{"given":"Chen","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shubin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuejia","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhao","family":"Qi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binghui","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jixiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanglong","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuling","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kegong","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaobin","family":"Shang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kewei","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianzhong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1909-8760","authenticated-orcid":false,"given":"Nanhua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2023.109847_bib1","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1002\/eji.201243088","article-title":"Conditional ligands for Asian HLA variants facilitate the definition of CD8(+) T-cell responses in acute and chronic viral diseases","volume":"43","author":"Chang","year":"2013","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.vetmic.2023.109847_bib2","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1099\/vir.0.027995-0","article-title":"Emergence of novel European genotype porcine reproductive and respiratory syndrome virus in mainland China","volume":"92","author":"Chen","year":"2011","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.vetmic.2023.109847_bib3","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s13567-021-00944-8","article-title":"Chimeric HP-PRRSV2 containing an ORF2-6 consensus sequence induces antibodies with broadly neutralizing activity and confers cross protection against virulent NADC30-like isolate","volume":"52","author":"Chen","year":"2021","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetmic.2023.109847_bib4","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.meegid.2017.07.024","article-title":"Whole genome characterization of a novel porcine reproductive and respiratory syndrome virus 1 isolate: Genetic evidence for recombination between Amervac vaccine and circulating strains in mainland China","volume":"54","author":"Chen","year":"2017","journal-title":"Infect. Genet. Evol.: J. Mol. Epidemiol. Evolut. Genet. Infect. Dis."},{"key":"10.1016\/j.vetmic.2023.109847_bib5","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.rvsc.2020.04.004","article-title":"High genetic diversity of Chinese porcine reproductive and respiratory syndrome viruses from 2016 to 2019","volume":"131","author":"Chen","year":"2020","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.vetmic.2023.109847_bib6","doi-asserted-by":"crossref","first-page":"2271","DOI":"10.1111\/tbed.13276","article-title":"Development of universal and quadruplex real-time RT-PCR assays for simultaneous detection and differentiation of porcine reproductive and respiratory syndrome viruses","volume":"66","author":"Chen","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.vetmic.2023.109847_bib7","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.vetmic.2016.06.003","article-title":"Comparison of protection provided by type 1 and type 2 porcine reproductive and respiratory syndrome field viruses against homologous and heterologous challenge","volume":"191","author":"Choi","year":"2016","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2023.109847_bib8","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1177\/104063879200400201","article-title":"Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs","volume":"4","author":"Collins","year":"1992","journal-title":"J. Vet. Diagn. Investig.: Off. Publ. Am. Assoc. Vet. Lab. Diagn., Inc."},{"key":"10.1016\/j.vetmic.2023.109847_bib9","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1297-9716-43-30","article-title":"Characterization of homologous and heterologous adaptive immune responses in porcine reproductive and respiratory syndrome virus infection","volume":"43","author":"Diaz","year":"2012","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetmic.2023.109847_bib10","first-page":"1","article-title":"Isolation and identification of porcine reproductive and respiratory syndrome (PRRS) virus from aborted fetuses suspected of \"PRRS\"","volume":"2","author":"Guo","year":"1996","journal-title":"Chin. J. Prev. Vet. Med."},{"key":"10.1016\/j.vetmic.2023.109847_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2022.104462","article-title":"Identification of CD4(+) T cells with T follicular helper cell characteristics in the pig.","volume":"134","author":"Hoog","year":"2022","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.vetmic.2023.109847_bib12","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0378-1135(03)00157-3","article-title":"Respiratory tract protection upon challenge of pigs vaccinated with attenuated porcine reproductive and respiratory syndrome virus vaccines","volume":"95","author":"Labarque","year":"2003","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2023.109847_bib13","doi-asserted-by":"crossref","unstructured":"Li, C., Qiu, M., Li, S.B., Sun, Z., Huang, Z.T., Qi, W.H., Qiu, Y.J., Li, J.X., Feng, B.H., Zhao, D.S., Lin, H., Zheng, W.L., Yu, X.L., Tian, K.G., Fan, K.W., Zhu, J.Z., Chen, N.H., 2023a. Metagenomic and Pathogenic Assessments Identify a Pathogenic Porcine Reproductive and Respiratory Syndrome Virus 1 with New Deletions from Adult Slaughter Pig in 2022. Transboundary and emerging diseases 2023.","DOI":"10.1155\/2023\/1975039"},{"key":"10.1016\/j.vetmic.2023.109847_bib14","doi-asserted-by":"crossref","DOI":"10.1099\/jgv.0.001850","article-title":"A potent CD8 T-cell response may be associated with partial cross-protection conferred by an attenuated Chinese HP-PRRSV vaccine against NADC30-like PRRSV challenge","volume":"104","author":"Li","year":"2023","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.vetmic.2023.109847_bib15","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.vetimm.2004.09.005","article-title":"Role of neutralizing antibodies in PRRSV protective immunity","volume":"102","author":"Lopez","year":"2004","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetmic.2023.109847_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetimm.2015.07.003","article-title":"Innate and adaptive immunity against porcine reproductive and respiratory syndrome virus","volume":"167","author":"Loving","year":"2015","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetmic.2023.109847_bib17","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1146\/annurev-animal-022114-111025","article-title":"Porcine reproductive and respiratory syndrome virus (PRRSV): pathogenesis and interaction with the immune system","volume":"4","author":"Lunney","year":"2016","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"10.1016\/j.vetmic.2023.109847_bib18","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0378-1135(02)00426-1","article-title":"Comparative safety and efficacy of attenuated single-strain and multi-strain vaccines for porcine reproductive and respiratory syndrome","volume":"93","author":"Mengeling","year":"2003","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2023.109847_bib19","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.prevetmed.2017.04.006","article-title":"Cost of porcine reproductive and respiratory syndrome virus at individual farm level - an economic disease model","volume":"142","author":"Nathues","year":"2017","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.vetmic.2023.109847_bib20","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s13567-020-00789-7","article-title":"Evaluation of local and systemic immune responses in pigs experimentally challenged with porcine reproductive and respiratory syndrome virus","volume":"51","author":"Nazki","year":"2020","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetmic.2023.109847_bib21","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.vetmic.2014.05.030","article-title":"Evaluation of the efficacy of a new modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine (Fostera PRRS) against heterologous PRRSV challenge","volume":"172","author":"Park","year":"2014","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2023.109847_bib22","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.02574-21","article-title":"Systemic homologous neutralizing antibodies are inadequate for the evaluation of vaccine protective efficacy against coinfection by high virulent PEDV and PRRSV","volume":"10","author":"Qiu","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.vetmic.2023.109847_bib23","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.vetmic.2017.04.021","article-title":"Preparation for emergence of an Eastern European porcine reproductive and respiratory syndrome virus (PRRSV) strain in Western Europe: Immunization with modified live virus vaccines or a field strain confers partial protection","volume":"204","author":"Renson","year":"2017","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2023.109847_bib24","doi-asserted-by":"crossref","first-page":"4335","DOI":"10.1016\/j.vaccine.2016.06.069","article-title":"Attempts to enhance cross-protection against porcine reproductive and respiratory syndrome viruses using chimeric viruses containing structural genes from two antigenically distinct strains","volume":"34","author":"Sun","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.vetmic.2023.109847_bib25","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2022.974743","article-title":"Emergence of a novel PRRSV-1 strain in mainland China: a recombinant strain derived from the two commercial modified live viruses Amervac and DV","volume":"9","author":"Sun","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.vetmic.2023.109847_bib26","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.virol.2015.08.021","article-title":"Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs","volume":"485","author":"Tian","year":"2015","journal-title":"Virology"},{"key":"10.1016\/j.vetmic.2023.109847_bib27","doi-asserted-by":"crossref","first-page":"6515","DOI":"10.1128\/JVI.03287-14","article-title":"A single amino acid deletion in the matrix protein of porcine reproductive and respiratory syndrome virus confers resistance to a polyclonal swine antibody with broadly neutralizing activity","volume":"89","author":"Trible","year":"2015","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2023.109847_bib28","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1038\/s41590-018-0068-4","article-title":"Recognition of microbial viability via TLR8 drives TFH cell differentiation and vaccine responses","volume":"19","author":"Ugolini","year":"2018","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.vetmic.2023.109847_bib29","doi-asserted-by":"crossref","first-page":"12070","DOI":"10.1128\/JVI.01657-15","article-title":"A synthetic porcine reproductive and respiratory syndrome virus strain confers unprecedented levels of heterologous protection","volume":"89","author":"Vu","year":"2015","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2023.109847_bib30","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.vetmic.2016.09.014","article-title":"Strategies to broaden the cross-protective efficacy of vaccines against porcine reproductive and respiratory syndrome virus","volume":"206","author":"Vu","year":"2017","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2023.109847_bib31","doi-asserted-by":"crossref","first-page":"2737","DOI":"10.1007\/s00705-020-04752-x","article-title":"Changes to virus taxonomy and the Statutes ratified by the international committee on taxonomy of viruses (2020)","volume":"165","author":"Walker","year":"2020","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetmic.2023.109847_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2023.106158","article-title":"Pathogenicity characterization of PRRSV-1 181187-2 isolated in China","volume":"180","author":"Wang","year":"2023","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.vetmic.2023.109847_bib33","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.virusres.2016.09.006","article-title":"Genomic characterization and pathogenicity of a strain of type 1 porcine reproductive and respiratory syndrome virus","volume":"225","author":"Wang","year":"2016","journal-title":"Virus Res."},{"key":"10.1016\/j.vetmic.2023.109847_bib34","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1080\/01652176.1991.9694296","article-title":"Mystery swine disease in The Netherlands: the isolation of Lelystad virus","volume":"13","author":"Wensvoort","year":"1991","journal-title":"Vet. Q."},{"key":"10.1016\/j.vetmic.2023.109847_bib35","doi-asserted-by":"crossref","unstructured":"Xu, H., Gong, B.J., Sun, Q., Li, C., Zhao, J., Xiang, L.R., Li, W.S., Guo, Z.Y., Tang, Y.D., Leng, C.L., Li, Z., Wang, Q., Zhou, G.H., An, T.Q., Cai, X.H., Tian, Z.J., Peng, J.M., Zhang, H.L., 2023. Genomic Characterization and Pathogenicity of BJEU06\u20131-Like PRRSV-1 ZD-1 Isolated in China. Transboundary and emerging diseases 2023.","DOI":"10.1155\/2023\/6793604"},{"key":"10.1016\/j.vetmic.2023.109847_bib36","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1007\/s00705-018-3873-z","article-title":"Phylogeographic analysis of porcine reproductive and respiratory syndrome virus 1 in Guangdong province, Southern China","volume":"163","author":"Zhai","year":"2018","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetmic.2023.109847_bib37","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/s41564-020-00824-5","article-title":"Spike-specific circulating T follicular helper cell and cross-neutralizing antibody responses in COVID-19-convalescent individuals","volume":"6","author":"Zhang","year":"2021","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.vetmic.2023.109847_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2020.104531","article-title":"Genetic analysis of a porcine reproductive and respiratory syndrome virus 1 strain in China with new patterns of amino acid deletions in nsp2, GP3 and GP4","volume":"149","author":"Zhang","year":"2020","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.vetmic.2023.109847_bib39","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s13567-021-00914-0","article-title":"A broadly neutralizing monoclonal antibody induces broad protection against heterogeneous PRRSV strains in piglets","volume":"52","author":"Zhang","year":"2021","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetmic.2023.109847_bib40","doi-asserted-by":"crossref","first-page":"362","DOI":"10.3390\/vaccines9040362","article-title":"Porcine reproductive and respiratory syndrome modified live virus vaccine: a \"leaky\" vaccine with debatable efficacy and safety","volume":"9","author":"Zhou","year":"2021","journal-title":"Vaccines"}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113523001992?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113523001992?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T23:21:07Z","timestamp":1759879267000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113523001992"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10]]},"references-count":40,"alternative-id":["S0378113523001992"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2023.109847","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2023,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Efficacy of a porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) natural recombinant against a heterologous PRRSV-1 isolate both clustered within the subgroup of BJEU06-1-like isolates","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2023.109847","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109847"}}