{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T05:16:58Z","timestamp":1731734218761,"version":"3.28.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T00:00:00Z","timestamp":1723680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2024,11]]},"DOI":"10.1016\/j.vetmic.2024.110222","type":"journal-article","created":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T15:27:24Z","timestamp":1723735644000},"page":"110222","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Monitoring of pathogenic Leptospira infection in wolves (Canis lupus) from Spain and Italy"],"prefix":"10.1016","volume":"298","author":[{"given":"Mois\u00e9s","family":"Gonz\u00e1lvez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Cano-Terriza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manena","family":"Fayos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Moroni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Remigio","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serena","family":"Robetto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1lvaro","family":"Oleaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Remesar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riccardo","family":"Orusa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Mu\u00f1oz-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roser","family":"Velarde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ignacio","family":"Garc\u00eda-Bocanegra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2024.110222_bib1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.vetmic.2009.03.012","article-title":"Leptospira and leptospirosis","volume":"40","author":"Adler","year":"2010","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2024.110222_bib2","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/1476-0711-5-28","article-title":"Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species","volume":"5","author":"Ahmed","year":"2006","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"10.1016\/j.vetmic.2024.110222_bib3","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0007093","article-title":"Development and validation of a real-time PCR for detection of pathogenic Leptospira species in clinical materials","volume":"4","author":"Ahmed","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.vetmic.2024.110222_bib4","doi-asserted-by":"crossref","first-page":"474","DOI":"10.7589\/0090-3558-46.2.474","article-title":"Serosurvey for canine distemper virus, canine adenovirus, Leptospira interrogans, and Toxoplasma gondii in free-ranging canids in Scandinavia and Svalbard","volume":"46","author":"Akerstedt","year":"2010","journal-title":"J. Wildl. Dis."},{"key":"10.1016\/j.vetmic.2024.110222_bib5","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S1473-3099(03)00830-2","article-title":"Leptospirosis: a zoonotic disease of global importance","volume":"3","author":"Bharti","year":"2003","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.vetmic.2024.110222_bib6","series-title":"A report of the N2K group for DG environment","article-title":"The situation of the wolf (Canis lupus) in the European Union \u2013 an in-depth analysis","author":"Blanco","year":"2023"},{"key":"10.1016\/j.vetmic.2024.110222_bib7","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.3390\/pathogens12091167","article-title":"Leptospirosis and the environment: a review and future directions","volume":"12","author":"Bradley","year":"2023","journal-title":"Pathogens"},{"key":"10.1016\/j.vetmic.2024.110222_bib8","first-page":"1","article-title":"A visual guide to wolf dentition and age determination","author":"Brasington","year":"2023","journal-title":"Wildl. Biol."},{"key":"10.1016\/j.vetmic.2024.110222_bib9","doi-asserted-by":"crossref","first-page":"2512","DOI":"10.3390\/ijerph18052512","article-title":"Environmental exposure of wild carnivores to zoonotic pathogens: Leptospira infection in the first free living wolf (Canis lupus Linnaeus, 1758) found dead in the Friuli Venezia Giulia Region","volume":"18","author":"Bregoli","year":"2021","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.vetmic.2024.110222_bib10","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1126\/science.1257553","article-title":"Recovery of large carnivores in Europe's modern human-dominated landscapes","volume":"346","author":"Chapr\u00f3n","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.vetmic.2024.110222_bib11","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1038\/nmeth.2109","article-title":"jModelTest 2: more models, new heuristics and parallel computing","volume":"9","author":"Darriba","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.vetmic.2024.110222_bib12","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1017\/S0950268899003969","article-title":"Serological prevalence to six leptospiral serovars in cattle in Asturias (Northern Spain)","volume":"124","author":"Esp\u00ed","year":"2000","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.vetmic.2024.110222_bib13","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.tvjl.2008.10.003","article-title":"Leptospiral antibodies in Iberian red deer (Cervus elaphus hispanicus), fallow deer (Dama dama) and European wild boar (Sus scrofa) in Asturias, Northern Spain","volume":"183","author":"Esp\u00ed","year":"2010","journal-title":"Vet. J."},{"key":"10.1016\/j.vetmic.2024.110222_bib14","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.mambio.2019.10.008","article-title":"Food habits of wolves and selection of wild ungulates in a prey-rich Mediterranean coastal area","volume":"99","author":"Ferretti","year":"2019","journal-title":"Mamm. Biol."},{"key":"10.1016\/j.vetmic.2024.110222_bib15","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1007\/BF02101694","article-title":"Dating of the human-ape splitting by a molecular clock of mitochondrial DNA","volume":"22","author":"Hasegawa","year":"1985","journal-title":"J. Mol. Evol."},{"key":"10.1016\/j.vetmic.2024.110222_bib16","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/jvim.16309","article-title":"Evaluation by polymerase chain reaction assay of persistent shedding of pathogenic leptospires in the urine of dogs with leptospirosis","volume":"36","author":"Hetrick","year":"2022","journal-title":"J. Vet. Intern. Med."},{"key":"10.1016\/j.vetmic.2024.110222_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.fooweb.2022.e00248","article-title":"Grey wolf feeding habits and their geographical variation in Northwest Spain","volume":"32","author":"Janeiro-Otero","year":"2022","journal-title":"Food Webs"},{"key":"10.1016\/j.vetmic.2024.110222_bib18","doi-asserted-by":"crossref","first-page":"248","DOI":"10.7589\/0090-3558-27.2.248","article-title":"Seroepidemiology of leptospirosis in Minnesota wolves","volume":"27","author":"Khan","year":"1991","journal-title":"J. Wildl. Dis."},{"key":"10.1016\/j.vetmic.2024.110222_bib19","doi-asserted-by":"crossref","DOI":"10.1038\/srep41730","article-title":"Population expansion and individual age affect endoparasite richness and diversity in a recolonising large carnivore population","volume":"7","author":"Lesniak","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.vetmic.2024.110222_bib20","doi-asserted-by":"crossref","first-page":"2160","DOI":"10.1002\/ece3.3839","article-title":"Recolonizing gray wolves increase parasite infection risk in their prey","volume":"8","author":"Lesniak","year":"2018","journal-title":"Ecol. Evol."},{"key":"10.1016\/j.vetmic.2024.110222_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2019.e02373","article-title":"Leptospira seroprevalence in owned dogs from Spain","volume":"5","author":"L\u00f3pez","year":"2019","journal-title":"Heliyon"},{"key":"10.1016\/j.vetmic.2024.110222_bib22","doi-asserted-by":"crossref","first-page":"3783","DOI":"10.3390\/ijerph20053783","article-title":"Synanthropic and wild animals as sentinels of zoonotic agents: a study of Leptospira genotypes circulating in Northeastern Italy","volume":"20","author":"Mazzotta","year":"2023","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.vetmic.2024.110222_bib23","doi-asserted-by":"crossref","first-page":"42","DOI":"10.5326\/JAAHA-MS-6882","article-title":"Persistent leptospiruria in five dogs despite antimicrobial treatment (2000-2017)","volume":"55","author":"Mauro","year":"2019","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"10.1016\/j.vetmic.2024.110222_bib24","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1590\/0074-0276140258","article-title":"Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires","volume":"109","author":"Mill\u00e1n","year":"2014","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.vetmic.2024.110222_bib25","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1089\/vbz.2008.0081","article-title":"Leptospirosis in wild and domestic carnivores in natural areas in Andalusia, Spain","volume":"9","author":"Mill\u00e1n","year":"2009","journal-title":"Vector Borne Zoonotic Dis."},{"key":"10.1016\/j.vetmic.2024.110222_bib26","first-page":"589","article-title":"Carriage of pathogenic Leptospira in carnivores at the wild\/domestic interface","volume":"22","author":"Mill\u00e1n","year":"2019","journal-title":"Pol. J. Vet. Sci."},{"key":"10.1016\/j.vetmic.2024.110222_bib27","doi-asserted-by":"crossref","DOI":"10.3389\/fcosc.2021.710218","article-title":"Welcoming wolves? Governing the return of large carnivores in traditional pastoral landscapes","volume":"2","author":"Pettersson","year":"2021","journal-title":"Front. Conserv. Sci."},{"unstructured":"RENAVE, 2024. Informaci\u00f3n Vigilancia en Salud P\u00fablica. Leptospirosis. [Accessed: 5 Jan 2024]. Available at: https:\/\/www.isciii.es\/QueHacemos\/Servicios\/VigilanciaSaludPublicaRENAVE\/Paginas\/default.aspx.","key":"10.1016\/j.vetmic.2024.110222_bib28"},{"key":"10.1016\/j.vetmic.2024.110222_bib29","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1093\/sysbio\/sys029","article-title":"MRBAYES 3.2 Efficient Bayesian phylogenetic inference and model selection across a large model space","volume":"61","author":"Ronquist","year":"2012","journal-title":"Syst. Biol."},{"key":"10.1016\/j.vetmic.2024.110222_bib30","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.diagmicrobio.2009.03.014","article-title":"Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene","volume":"64","author":"Stoddard","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"year":"2018","author":"Thrusfield","series-title":"Veterinary Epidemiology","key":"10.1016\/j.vetmic.2024.110222_bib31"},{"key":"10.1016\/j.vetmic.2024.110222_bib32","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s11250-017-1429-y","article-title":"A systematic review of leptospirosis on wild animals in Latin America","volume":"50","author":"Vieira","year":"2018","journal-title":"Trop. Anim. Health Prod."},{"key":"10.1016\/j.vetmic.2024.110222_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.ttbdis.2023.102272","article-title":"Tick-borne infections in wolves from an expanding population in Eastern Europe","volume":"15","author":"Wymaza\u0142","year":"2024","journal-title":"Ticks Tick. Borne Dis."},{"key":"10.1016\/j.vetmic.2024.110222_bib34","doi-asserted-by":"crossref","first-page":"77","DOI":"10.7589\/0090-3558-23.1.77","article-title":"Serologic survey for selected microbial pathogens of wolves in Alaska, 1975-1982","volume":"23","author":"Zarnke","year":"1987","journal-title":"J. Wildl. Dis."},{"key":"10.1016\/j.vetmic.2024.110222_bib35","doi-asserted-by":"crossref","first-page":"2722","DOI":"10.3390\/ani11092722","article-title":"Exposure of free-Ranging wild animals to zoonotic Leptospira interrogans sensu stricto in Slovenia","volume":"11","author":"\u017dele-Vengu\u0161t","year":"2021","journal-title":"Animals"}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S037811352400244X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S037811352400244X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T07:42:07Z","timestamp":1731656527000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S037811352400244X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":35,"alternative-id":["S037811352400244X"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2024.110222","relation":{},"ISSN":["0378-1135"],"issn-type":[{"type":"print","value":"0378-1135"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Monitoring of pathogenic Leptospira infection in wolves (Canis lupus) from Spain and Italy","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2024.110222","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"110222"}}