{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T20:17:55Z","timestamp":1786997875987,"version":"build-2736575974"},"reference-count":17,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2024,11]]},"DOI":"10.1016\/j.vetmic.2024.110268","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T18:59:14Z","timestamp":1727377154000},"page":"110268","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Assessment of real-time PCR test in oral fluid samples for screening the serotypes of Actinobacillus pleuropneumoniae circulating in swine herds"],"prefix":"10.1016","volume":"298","author":[{"given":"Jos\u00e9 Luis","family":"Arnal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Bel\u00e9n","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Lacotoure","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo \u00c1ngel","family":"Benito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sof\u00eda","family":"Lazaro-Gaspar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Gottschalk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2024.110268_bib1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.54846\/jshap\/524","article-title":"Dealing with unexpected Actinobacillus pleuropneumoniae serological results","volume":"15","author":"Broes","year":"2007","journal-title":"J. Swine Health Prod."},{"key":"10.1016\/j.vetmic.2024.110268_bib2","doi-asserted-by":"crossref","first-page":"283","DOI":"10.4142\/jvs.2017.18.3.283","article-title":"Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method","volume":"18","author":"Cheong","year":"2017","journal-title":"J. Vet. Sci."},{"key":"10.1016\/j.vetmic.2024.110268_bib3","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/S0378-1135(02)00150-5","article-title":"Evaluation of serology, bacteriological isolation and polymerase chain reaction for the detection of pigs carrying Actinobacillus pleuropneumoniae in the upper respiratory tract after experimental infection","volume":"88","author":"Chiers","year":"2002","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2024.110268_bib4","doi-asserted-by":"crossref","unstructured":"Costa, G., Oliveira, S., Torrison, J., 2012. Detection of Actinobacillus pleuropneumoniae in oral-fluid samples obtained from experimentally infected pigs. Journal of Swine Health and Production.","DOI":"10.54846\/jshap\/720"},{"key":"10.1016\/j.vetmic.2024.110268_bib5","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.vetmic.2010.08.023","article-title":"Evaluation of Actinobacillus pleuropneumoniae diagnostic tests using samples derived from experimentally infected pigs","volume":"148","author":"Costa","year":"2011","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2024.110268_bib6","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.tvjl.2015.06.016","article-title":"The challenge of detecting herds sub-clinically infected with Actinobacillus pleuropneumoniae","volume":"206","author":"Gottschalk","year":"2015","journal-title":"Vet. J."},{"key":"10.1016\/j.vetmic.2024.110268_bib7","doi-asserted-by":"crossref","unstructured":"Gottschalk, M., Broes, A., 2019. Actinobacillosis, in: Diseases of Swine. pp. 749\u2013766.","DOI":"10.1002\/9781119350927.ch48"},{"key":"10.1016\/j.vetmic.2024.110268_bib8","first-page":"62","article-title":"Long-chain LPS-based enzyme-linked immunosorbent assay to detect swine herds infected by Actinobacillus pleuropneumoniae serotype 17","volume":"62","author":"Gottschalk","year":"2021","journal-title":"Can. Vet. J."},{"key":"10.1016\/j.vetmic.2024.110268_bib9","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s40813-020-00168-w","article-title":"Guidelines for oral fluid-based surveillance of viral pathogens in swine","volume":"6","author":"Henao-Diaz","year":"2020","journal-title":"Porc. Health Manag."},{"key":"10.1016\/j.vetmic.2024.110268_bib10","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s40813-017-0055-4","article-title":"The use of oral fluids to monitor key pathogens in porcine respiratory disease complex","volume":"3","author":"Hernandez-Garcia","year":"2017","journal-title":"Porc. Health Manag."},{"key":"10.1016\/j.vetmic.2024.110268_bib11","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1292\/jvms.14-0174","article-title":"The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 14","volume":"77","author":"Ito","year":"2015","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.vetmic.2024.110268_bib12","first-page":"839","article-title":"Disease risks associated with free-ranging wild boar in Saskatchewan","volume":"56","author":"McGregor","year":"2015","journal-title":"Can. Vet. J."},{"key":"10.1016\/j.vetmic.2024.110268_bib13","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1177\/1040638712469607","article-title":"Evaluation of diagnostic assays for the serological detection of Actinobacillus pleuropneumoniae on samples of known or unknown exposure","volume":"25","author":"Opriessnig","year":"2013","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.vetmic.2024.110268_bib14","doi-asserted-by":"crossref","DOI":"10.1556\/004.2018.031","article-title":"Actinobacillus pleuropneumoniae serotypes in Hungary","author":"S\u00e1rk\u00f6zi","year":"2018","journal-title":"Acta Vet. Hung."},{"key":"10.1016\/j.vetmic.2024.110268_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2021.109021","article-title":"Proposal of Actinobacillus pleuropneumoniae serovar 19, and reformulation of previous multiplex PCRs for capsule-specific typing of all known serovars","volume":"255","author":"Stringer","year":"2021","journal-title":"Vet. Microbiol"},{"key":"10.1016\/j.vetmic.2024.110268_bib16","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.agsy.2016.02.005","article-title":"Economic value of mitigating Actinobacillus pleuropneumoniae infections in pig fattening herds","volume":"144","author":"Stygar","year":"2016","journal-title":"Agric. Syst."},{"key":"10.1016\/j.vetmic.2024.110268_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.tvjl.2012.02.004","article-title":"Detection of Actinobacillus pleuropneumoniae in pigs by real-time quantitative PCR for the apxIVA gene","author":"Tobias","year":"2012","journal-title":"Vet. J."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113524002906?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113524002906?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T02:43:26Z","timestamp":1731638606000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113524002906"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":17,"alternative-id":["S0378113524002906"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2024.110268","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Assessment of real-time PCR test in oral fluid samples for screening the serotypes of Actinobacillus pleuropneumoniae circulating in swine herds","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2024.110268","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110268"}}