{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T17:47:03Z","timestamp":1793382423002,"version":"4.5.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2022YFD1800300"],"award-info":[{"award-number":["2022YFD1800300"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32373020"],"award-info":[{"award-number":["32373020"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.vetmic.2025.110604","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T02:43:05Z","timestamp":1749782585000},"page":"110604","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Pseudorabies virus DNA binding to porcine NLRC3 unleashes the activation of the porcine NLRP3 inflammasome"],"prefix":"10.1016","volume":"307","author":[{"given":"Minjie","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lvye","family":"Chai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1636-8910","authenticated-orcid":false,"given":"Xin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.vetmic.2025.110604_bib1","doi-asserted-by":"crossref","DOI":"10.3390\/v14051003","article-title":"A Review of Pseudorabies Virus Variants: Genomics, Vaccination, Transmission, and Zoonotic Potential","volume":"14","author":"Bo","year":"2022","journal-title":"Viruses"},{"key":"10.1016\/j.vetmic.2025.110604_bib2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetmic.2016.11.019","article-title":"Vaccines against pseudorabies virus (PrV)","volume":"206","author":"Freuling","year":"2017","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2025.110604_bib3","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.vetmic.2016.01.010","article-title":"Antiviral activities of 2,6-diaminopurine-based acyclic nucleoside phosphonates against herpesviruses: In vitro study results with pseudorabies virus (PrV, SuHV-1)","volume":"184","author":"Zouharova","year":"2016","journal-title":"Vet. Microbiol."},{"issue":"8","key":"10.1016\/j.vetmic.2025.110604_bib4","doi-asserted-by":"crossref","DOI":"10.3390\/v14081638","article-title":"Pseudorabies Virus: From Pathogenesis to Prevention Strategies","volume":"14","author":"Zheng","year":"2022","journal-title":"Viruses"},{"issue":"3","key":"10.1016\/j.vetmic.2025.110604_bib5","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1128\/MMBR.69.3.462-500.2005","article-title":"Molecular Biology of Pseudorabies Virus: Impact on Neurovirology and Veterinary Medicine","volume":"69","author":"Pomeranz","year":"2005","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.vetmic.2025.110604_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2021.109130","article-title":"ATP-dependent activation of NLRP3 inflammasome in primary murine macrophages infected by pseudorabies virus","volume":"259","author":"Ye","year":"2021","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2025.110604_bib7","first-page":"6","article-title":"SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation","author":"Pan","year":"2021","journal-title":"Nat. Commun. 12(1) 4664"},{"issue":"4","key":"10.1016\/j.vetmic.2025.110604_bib8","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1099\/jgv.0.000401","article-title":"Beyond the inflammasome: regulatory NOD-like receptor modulation of the host immune response following virus exposure","volume":"97","author":"Coutermarsh-Ott","year":"2016","journal-title":"J. Gen. Virol."},{"issue":"11","key":"10.1016\/j.vetmic.2025.110604_bib9","doi-asserted-by":"crossref","first-page":"2288","DOI":"10.1016\/j.cell.2023.04.025","article-title":"A 360\u00b0 view of the inflammasome: Mechanisms of activation, cell death, and diseases","volume":"186","author":"Barnett","year":"2023","journal-title":"Cell"},{"issue":"2","key":"10.1016\/j.vetmic.2025.110604_bib10","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1189\/jlb.3MR0617-250R","article-title":"Molecular mechanisms of inflammasome signaling","volume":"103","author":"Mathur","year":"2018","journal-title":"J. Leukoc. Biol."},{"issue":"6","key":"10.1016\/j.vetmic.2025.110604_bib11","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/nri3452","article-title":"Activation and regulation of the inflammasomes","volume":"13","author":"Latz","year":"2013","journal-title":"Nat. Rev. Immunol."},{"issue":"2","key":"10.1016\/j.vetmic.2025.110604_bib12","doi-asserted-by":"crossref","first-page":"787","DOI":"10.4049\/jimmunol.0901363","article-title":"Cutting Edge: NF-\u03baB Activating Pattern Recognition and Cytokine Receptors License NLRP3 Inflammasome Activation by Regulating NLRP3 Expression","volume":"183","author":"Bauernfeind","year":"2009","journal-title":"J. Immunol."},{"issue":"4","key":"10.1016\/j.vetmic.2025.110604_bib13","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/s41422-020-0295-8","article-title":"Toward targeting inflammasomes: insights into their regulation and activation","volume":"30","author":"Christgen","year":"2020","journal-title":"Cell Res."},{"issue":"2","key":"10.1016\/j.vetmic.2025.110604_bib14","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.aag2045","article-title":"ZBP1\/DAI is an innate sensor of influenza virus triggering the NLRP3 inflammasome and programmed cell death pathways","volume":"1","author":"Kuriakose","year":"2016","journal-title":"Sci. Immunol."},{"issue":"7293","key":"10.1016\/j.vetmic.2025.110604_bib15","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1038\/nature08938","article-title":"NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals","volume":"464","author":"Duewell","year":"2010","journal-title":"Nature"},{"issue":"22","key":"10.1016\/j.vetmic.2025.110604_bib16","doi-asserted-by":"crossref","DOI":"10.21037\/atm-22-4120","article-title":"NLRP3 mediates trophoblastic inflammasome activation and protects against Listeria monocytogenes infection during pregnancy","volume":"10","author":"Gao","year":"2022","journal-title":"Ann. Transl. Med."},{"key":"10.1016\/j.vetmic.2025.110604_bib17","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.2147\/DDDT.S356307","article-title":"Palmatine Protects Against MSU-Induced Gouty Arthritis via Regulating the NF-\u03baB\/NLRP3 and Nrf2 Pathways","volume":"16","author":"Cheng","year":"2022","journal-title":"Drug Des. Dev. Ther."},{"issue":"4","key":"10.1016\/j.vetmic.2025.110604_bib18","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/j.immuni.2009.02.005","article-title":"The NLRP3 Inflammasome Mediates In Vivo Innate Immunity to Influenza A Virus through Recognition of Viral RNA","volume":"30","author":"Allen","year":"2009","journal-title":"Immunity"},{"key":"10.1016\/j.vetmic.2025.110604_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.neulet.2020.135279","article-title":"Beta-amyloid activates NLRP3 inflammasome via TLR4 in mouse microglia","volume":"736","author":"Liu","year":"2020","journal-title":"Neurosci. Lett."},{"issue":"50","key":"10.1016\/j.vetmic.2025.110604_bib20","doi-asserted-by":"crossref","first-page":"41732","DOI":"10.1074\/jbc.M112.381228","article-title":"Multiple Binding Sites on the Pyrin Domain of ASC Protein Allow Self-association and Interaction with NLRP3 Protein","volume":"287","author":"Vajjhala","year":"2012","journal-title":"J. Biol. Chem."},{"issue":"7575","key":"10.1016\/j.vetmic.2025.110604_bib21","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1038\/nature15514","article-title":"Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death","volume":"526","author":"Shi","year":"2015","journal-title":"Nature"},{"issue":"3","key":"10.1016\/j.vetmic.2025.110604_bib22","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.immuni.2014.01.010","article-title":"NLRC3, a Member of the NLR Family of Proteins, Is a Negative Regulator of Innate Immune Signaling Induced by the DNA Sensor STING","volume":"40","author":"Zhang","year":"2014","journal-title":"Immunity"},{"issue":"3","key":"10.1016\/j.vetmic.2025.110604_bib23","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.immuni.2019.02.009","article-title":"Viral DNA Binding to NLRC3, an Inhibitory Nucleic Acid Sensor, Unleashes STING, a Cyclic Dinucleotide Receptor that Activates Type I Interferon","volume":"50","author":"Li","year":"2019","journal-title":"Immunity"},{"issue":"11","key":"10.1016\/j.vetmic.2025.110604_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2024.111145","article-title":"Porcine NLRC3 specially binds short dsDNA to regulate cGAS activation","volume":"27","author":"Li","year":"2024","journal-title":"iScience"},{"issue":"8","key":"10.1016\/j.vetmic.2025.110604_bib25","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.it.2005.06.004","article-title":"NLRs join TLRs as innate sensors of pathogens","volume":"26","author":"Martinon","year":"2005","journal-title":"Trends Immunol."},{"issue":"1","key":"10.1016\/j.vetmic.2025.110604_bib26","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.coi.2008.01.003","article-title":"NLR, the nucleotide-binding domain leucine-rich repeat containing gene family","volume":"20","author":"Ye","year":"2008","journal-title":"Curr. Opin. Immunol."},{"issue":"9","key":"10.1016\/j.vetmic.2025.110604_bib27","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1038\/ni.2378","article-title":"The innate immune sensor NLRC3 attenuates Toll-like receptor signaling via modification of the signaling adaptor TRAF6 and transcription factor NF-\u03baB","volume":"13","author":"Schneider","year":"2012","journal-title":"Nat. Immunol."},{"issue":"1","key":"10.1016\/j.vetmic.2025.110604_bib28","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.ymthe.2022.08.023","article-title":"NLRC3 expression in macrophage impairs glycolysis and host immune defense by modulating the NF-\u03baB-NFAT5 complex during septic immunosuppression","volume":"31","author":"Xu","year":"2023","journal-title":"Mol. Ther."},{"issue":"7634","key":"10.1016\/j.vetmic.2025.110604_bib29","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/nature20597","article-title":"NLRC3 is an inhibitory sensor of PI3K\u2013mTOR pathways in cancer","volume":"540","author":"Karki","year":"2016","journal-title":"Nature"},{"issue":"13","key":"10.1016\/j.vetmic.2025.110604_bib30","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1080\/15384101.2017.1317414","article-title":"NLRC3 regulates cellular proliferation and apoptosis to attenuate the development of colorectal cancer","volume":"16","author":"Karki","year":"2017","journal-title":"Cell Cycle"},{"issue":"8","key":"10.1016\/j.vetmic.2025.110604_bib31","doi-asserted-by":"crossref","first-page":"2896","DOI":"10.4049\/jimmunol.1601370","article-title":"The Scaffolding Protein IQGAP1 Interacts with NLRC3 and Inhibits Type I IFN Production","volume":"199","author":"Tocker","year":"2017","journal-title":"J. Immunol."},{"issue":"30","key":"10.1016\/j.vetmic.2025.110604_bib32","doi-asserted-by":"crossref","first-page":"12691","DOI":"10.1074\/jbc.M116.769695","article-title":"NLRC3 protein inhibits inflammation by disrupting NALP3 inflammasome assembly via competition with the adaptor protein ASC for pro-caspase-1 binding","volume":"292","author":"Eren","year":"2017","journal-title":"J. Biol. Chem."},{"issue":"15","key":"10.1016\/j.vetmic.2025.110604_bib33","doi-asserted-by":"crossref","first-page":"12174","DOI":"10.18632\/oncotarget.24040","article-title":"Porcine alveolar macrophage CD163 abundance is a pivotal switch for porcine reproductive and respiratory syndrome virus infection","volume":"9","author":"Wang","year":"2018","journal-title":"Oncotarget"},{"issue":"7","key":"10.1016\/j.vetmic.2025.110604_bib34","article-title":"Species-specific IL-1\u03b2 is an inflammatory sensor of Seneca Valley Virus 3C Protease","volume":"20","author":"Sarnow","year":"2024","journal-title":"PLOS Pathog."},{"issue":"8","key":"10.1016\/j.vetmic.2025.110604_bib35","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23084469","article-title":"Coinfection of Porcine Circovirus 2 and Pseudorabies Virus Enhances Immunosuppression and Inflammation through NF-\u03baB, JAK\/STAT, MAPK, and NLRP3 Pathways","volume":"23","author":"Li","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"3","key":"10.1016\/j.vetmic.2025.110604_bib36","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1128\/jvi.00003-23","article-title":"Pseudorabies Virus Infection Activates the TLR-NF-\u03baB Axis and AIM2 Inflammasome To Enhance Inflammatory Responses in Mice","volume":"97","author":"Zhou","year":"2023","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2025.110604_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.virol.2024.110214","article-title":"Pseudorabies virus UL13 primes inflammatory response through downregulating heat shock factor 1","volume":"600","author":"Zhang","year":"2024","journal-title":"Virology"},{"issue":"9","key":"10.1016\/j.vetmic.2025.110604_bib38","article-title":"Pseudorabies Virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation","volume":"20","author":"Kalejta","year":"2024","journal-title":"PLOS Pathog."},{"issue":"12","key":"10.1016\/j.vetmic.2025.110604_bib39","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.01945-24","article-title":"Suppression of ZBP1-mediated NLRP3 inflammasome by the tegument protein VP22 facilitates pseudorabies virus infection","volume":"15","author":"Ma","year":"2024","journal-title":"mBio"},{"issue":"1","key":"10.1016\/j.vetmic.2025.110604_bib40","article-title":"EV71 3D Protein Binds with NLRP3 and Enhances the Assembly of Inflammasome Complex","volume":"13","author":"Luo","year":"2017","journal-title":"PLOS Pathog."},{"issue":"1","key":"10.1016\/j.vetmic.2025.110604_bib41","article-title":"Zika virus infection induces host inflammatory responses by facilitating NLRP3 inflammasome assembly and interleukin-1\u03b2 secretion","volume":"9","author":"Wang","year":"2018","journal-title":"Nat. Commun."},{"issue":"7081","key":"10.1016\/j.vetmic.2025.110604_bib42","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1038\/nature04517","article-title":"Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin\/Nalp3","volume":"440","author":"Kanneganti","year":"2006","journal-title":"Nature"},{"issue":"48","key":"10.1016\/j.vetmic.2025.110604_bib43","doi-asserted-by":"crossref","first-page":"36560","DOI":"10.1074\/jbc.M607594200","article-title":"Critical Role for Cryopyrin\/Nalp3 in Activation of Caspase-1 in Response to Viral Infection and Double-stranded RNA","volume":"281","author":"Kanneganti","year":"2006","journal-title":"J. Biol. Chem."},{"issue":"4","key":"10.1016\/j.vetmic.2025.110604_bib44","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.immuni.2009.02.006","article-title":"The Intracellular Sensor NLRP3 Mediates Key Innate and Healing Responses to Influenza A Virus via the Regulation of Caspase-1","volume":"30","author":"Thomas","year":"2009","journal-title":"Immunity"},{"key":"10.1016\/j.vetmic.2025.110604_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2023.109826","article-title":"Pseudorabies virus infection activates the NLRP3 and IFI16 inflammasomes to trigger pyroptosis","volume":"284","author":"Zhang","year":"2023","journal-title":"Vet. Microbiol."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113525002391?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113525002391?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T01:01:21Z","timestamp":1762304481000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113525002391"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":45,"alternative-id":["S0378113525002391"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2025.110604","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Pseudorabies virus DNA binding to porcine NLRC3 unleashes the activation of the porcine NLRP3 inflammasome","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2025.110604","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110604"}}