{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T21:07:04Z","timestamp":1773868024486,"version":"3.50.1"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001458","name":"American Kennel Club Canine Health Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001458","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001704","name":"European Society of Clinical Microbiology and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015900","name":"OVC Pet Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015900","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100029348","name":"American Kennel Club Humane Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100029348","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.vetmic.2025.110808","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T12:20:55Z","timestamp":1763641255000},"page":"110808","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Antimicrobial susceptibility and genomic determinants of resistance and virulence in Mycoplasma cynos and Mycoplasma felis"],"prefix":"10.1016","volume":"312","author":[{"given":"Isaac","family":"Framst","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael L.","family":"Beeton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley W.","family":"Peterson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeff L.","family":"Caswell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grazieli","family":"Maboni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"12","key":"10.1016\/j.vetmic.2025.110808_bib1","doi-asserted-by":"crossref","first-page":"6281","DOI":"10.3382\/ps\/pez576","article-title":"In vitro evaluation of various antimicrobials against field mycoplasma gallisepticum and mycoplasma synoviae isolates in Egypt","volume":"98","author":"Abd El-Hamid","year":"2019","journal-title":"Poult. Sci."},{"issue":"6","key":"10.1016\/j.vetmic.2025.110808_bib2","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01974-21","article-title":"Beware of mycoplasma anti-immunoglobulin strategies","volume":"12","author":"Arfi","year":"2021","journal-title":"mBio"},{"issue":"2","key":"10.1016\/j.vetmic.2025.110808_bib3","doi-asserted-by":"crossref","DOI":"10.1128\/Spectrum.00262-21","article-title":"Genome-wide association study reveals genetic markers for antimicrobial resistance in Mycoplasma bovis","volume":"9","author":"Bokma","year":"2021","journal-title":"Microbiol. Spectr."},{"issue":"10","key":"10.1016\/j.vetmic.2025.110808_bib4","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1099\/mic.0.26848-0","article-title":"Mycoplasmas associated with canine infectious respiratory disease","volume":"150","author":"Chalker","year":"2004","journal-title":"Microbiology"},{"issue":"2","key":"10.1016\/j.vetmic.2025.110808_bib5","first-page":"24","article-title":"Mycoplasmas and their antibiotic resistance: the problems and prospects in controlling infections","volume":"8","author":"Chernova","year":"2016","journal-title":"Actanaturae"},{"key":"10.1016\/j.vetmic.2025.110808_bib6","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.jcpa.2020.02.005","article-title":"Aetiology of canine infectious respiratory disease complex and prevalence of its pathogens in Europe","volume":"176","author":"Day","year":"2020","journal-title":"J. Comp. Pathol."},{"issue":"4","key":"10.1016\/j.vetmic.2025.110808_bib7","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1177\/1098612X16634387","article-title":"Prevalence of feline herpesvirus-1, feline calicivirus, Chlamydophila felis and Mycoplasma felis DNA and associated risk factors in cats in Spain with upper respiratory tract disease, conjunctivitis and\/or gingivostomatitis","volume":"19","author":"Fernandez","year":"2017","journal-title":"J. Feline Med. Surg."},{"issue":"11","key":"10.1016\/j.vetmic.2025.110808_bib8","doi-asserted-by":"crossref","DOI":"10.1099\/mic.0.001249","article-title":"Development of a long-read next generation sequencing workflow for improved characterization of fastidious respiratory mycoplasmas","volume":"168","author":"Framst","year":"2022","journal-title":"Microbiology"},{"issue":"4","key":"10.1016\/j.vetmic.2025.110808_bib9","doi-asserted-by":"crossref","DOI":"10.1128\/mra.01243-23","article-title":"Complete genome sequences of Mycoplasma cynos and Mycoplasma felis isolated from dogs and cats with infectious respiratory disease","volume":"13","author":"Framst","year":"2024","journal-title":"Microbiol. Resour. Announc."},{"key":"10.1016\/j.vetmic.2025.110808_bib10","series-title":"Mycoplasma spp.\" United States","first-page":"425","article-title":"Antimicrobial Resistance in","author":"Gautier-Bouchardon","year":"2018"},{"issue":"4","key":"10.1016\/j.vetmic.2025.110808_bib11","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1051\/vetres:2000100","article-title":"Guidelines and recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary mycoplasma species. International Research Programme on Comparative Mycoplasmology","volume":"31","author":"Hannan","year":"2000","journal-title":"Vet. Res."},{"issue":"5","key":"10.1016\/j.vetmic.2025.110808_bib12","doi-asserted-by":"crossref","first-page":"1880","DOI":"10.1111\/jvim.15568","article-title":"A systematic review and meta-analyses of the association between 4 mycoplasma species and lower respiratory tract disease in dogs","volume":"33","author":"Jambhekar","year":"2019","journal-title":"J. Vet. Intern Med."},{"issue":"3","key":"10.1016\/j.vetmic.2025.110808_bib13","article-title":"Unveiling genome plasticity and a novel phage in Mycoplasma felis: genomic investigations of four feline isolates","volume":"10","author":"Klose","year":"2024","journal-title":"Microb. Genom."},{"issue":"5","key":"10.1016\/j.vetmic.2025.110808_bib14","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/s41587-019-0072-8","article-title":"Assembly of long, error-prone reads using repeat graphs","volume":"37","author":"Kolmogorov","year":"2019","journal-title":"Nat. Biotechnol."},{"issue":"2","key":"10.1016\/j.vetmic.2025.110808_bib15","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1292\/jvms.15-0304","article-title":"Antimicrobial susceptibility and molecular characterization of macrolide resistance of Mycoplasma bovis isolates from multiple provinces in China","volume":"78","author":"Kong","year":"2016","journal-title":"J. Vet. Med. Sci."},{"issue":"2","key":"10.1016\/j.vetmic.2025.110808_bib16","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/jvim.14627","article-title":"Antimicrobial use guidelines for treatment of respiratory tract disease in dogs and cats: antimicrobial guidelines working group of the international society for companion animal infectious diseases","volume":"31","author":"Lappin","year":"2017","journal-title":"J. Vet. Intern Med"},{"issue":"4","key":"10.1016\/j.vetmic.2025.110808_bib17","doi-asserted-by":"crossref","first-page":"397","DOI":"10.2460\/javma.250.4.397","article-title":"A systematic review and meta-analysis of the association between Mycoplasma spp and upper and lower respiratory tract disease in cats","volume":"250","author":"Le Boedec","year":"2017","journal-title":"J. Am. Vet. Med. Assoc."},{"issue":"7","key":"10.1016\/j.vetmic.2025.110808_bib18","doi-asserted-by":"crossref","first-page":"588","DOI":"10.3390\/pathogens9070588","article-title":"Identification of antimicrobial resistance-associated genes through whole genome sequencing of mycoplasma bovis isolates with different antimicrobial resistances","volume":"9","author":"Ledger","year":"2020","journal-title":"Pathogens"},{"issue":"7","key":"10.1016\/j.vetmic.2025.110808_bib19","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1177\/1098612X14539087","article-title":"Feline respiratory disease: what is the role of Mycoplasma species?","volume":"16","author":"Lee-Fowler","year":"2014","journal-title":"J. Feline Med Surg."},{"issue":"6","key":"10.1016\/j.vetmic.2025.110808_bib20","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1038\/emboj.2009.17","article-title":"Erythromycin resistance by L4\/L22 mutations and resistance masking by drug efflux pump deficiency","volume":"28","author":"Lovmar","year":"2009","journal-title":"EMBO J."},{"key":"10.1016\/j.vetmic.2025.110808_bib21","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2023.1196747","article-title":"Drug sensitivity and genome-wide analysis of two strains of Mycoplasma gallisepticum with different biofilm intensity","volume":"14","author":"Ma","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.vetmic.2025.110808_bib22","doi-asserted-by":"crossref","first-page":"1455453","DOI":"10.3389\/fmicb.2024.1455453","article-title":"Feline respiratory disease complex: insights into the role of viral and bacterial co-infections","volume":"15","author":"Maboni","year":"2024","journal-title":"Front. Microbiol."},{"issue":"4","key":"10.1016\/j.vetmic.2025.110808_bib23","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0215817","article-title":"\"Canine infectious respiratory disease: new insights into the etiology and epidemiology of associated pathogens","volume":"14","author":"Maboni","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.vetmic.2025.110808_bib24","unstructured":"Methods for Antimicrobial Susceptibility Testing for Human Mycoplasmas; Approved Guideline., CLSI, Wayne, PA, 2011."},{"issue":"6","key":"10.1016\/j.vetmic.2025.110808_bib25","doi-asserted-by":"crossref","first-page":"2302","DOI":"10.1128\/AAC.49.6.2302-2306.2005","article-title":"Emergence of macrolide-resistant mycoplasma pneumoniae with a 23S rRNA gene mutation","volume":"49","author":"Morozumi","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"issue":"10","key":"10.1016\/j.vetmic.2025.110808_bib26","doi-asserted-by":"crossref","first-page":"3142","DOI":"10.1128\/AAC.46.10.3142-3150.2002","article-title":"Mutations in 23S rRNA account for intrinsic resistance to macrolides in mycoplasma hominis and mycoplasma fermentans and for acquired resistance to macrolides in M. hominis","volume":"46","author":"Pereyre","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"issue":"2","key":"10.1016\/j.vetmic.2025.110808_bib27","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ijantimicag.2006.09.008","article-title":"Characterisation of in vitro-selected mutants of Ureaplasma parvum resistant to macrolides and related antibiotics","volume":"29","author":"Pereyre","year":"2007","journal-title":"Int. J. Antimicrob. Agents"},{"issue":"2","key":"10.1016\/j.vetmic.2025.110808_bib28","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1177\/0300985813511130","article-title":"New and emerging pathogens in canine infectious respiratory disease","volume":"51","author":"Priestnall","year":"2014","journal-title":"Vet. Pathol."},{"issue":"1","key":"10.1016\/j.vetmic.2025.110808_bib29","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-020-17278-2","article-title":"Pathways for horizontal gene transfer in bacteria revealed by a global map of their plasmids","volume":"11","author":"Redondo-Salvo","year":"2020","journal-title":"Nat. Commun."},{"issue":"6","key":"10.1016\/j.vetmic.2025.110808_bib30","first-page":"462","article-title":"Experimental mycoplasmal pneumonia in dogs: electron microscopy of infected tissue","volume":"85B","author":"Rosendal","year":"1977","journal-title":"Acta Pathol. Microbiol. Scand. B"},{"issue":"11","key":"10.1016\/j.vetmic.2025.110808_bib31","article-title":"Bakta: rapid and standardized annotation of bacterial genomes via alignment-free sequence identification","volume":"7","author":"Schwengers","year":"2021","journal-title":"Microb. Genom."},{"issue":"3","key":"10.1016\/j.vetmic.2025.110808_bib32","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1177\/1040638711404155","article-title":"\"In vitro antimicrobial inhibition of Mycoplasma bovis isolates submitted to the Pennsylvania Animal Diagnostic Laboratory using flow cytometry and a broth microdilution method","volume":"23","author":"Soehnlen","year":"2011","journal-title":"J. Vet. Diagn. Invest"},{"issue":"2","key":"10.1016\/j.vetmic.2025.110808_bib33","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.01983-16","article-title":"Mutations associated with decreased susceptibility to seven antimicrobial families in field and laboratory-derived mycoplasma bovis strains","volume":"61","author":"Sulyok","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.vetmic.2025.110808_bib34","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.vetmic.2016.11.021","article-title":"Identification of genes involved in Mycoplasma gallisepticum biofilm formation using mini-Tn4001-SGM transposon mutagenesis","volume":"198","author":"Wang","year":"2017","journal-title":"Vet. Microbiol."},{"issue":"1","key":"10.1016\/j.vetmic.2025.110808_bib35","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-63136-y","article-title":"Cell surface processing of the P1 adhesin of Mycoplasma pneumoniae identifies novel domains that bind host molecules","volume":"10","author":"Widjaja","year":"2020","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.vetmic.2025.110808_bib36","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.gene.2014.12.036","article-title":"The identification of oppA gene homologues as part of the oligopeptide transport system in mycoplasmas","volume":"558","author":"Wium","year":"2015","journal-title":"Gene"},{"issue":"4","key":"10.1016\/j.vetmic.2025.110808_bib37","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1007\/s00253-014-6185-6","article-title":"Mycoplasma gallisepticum MGA_0676 is a membrane-associated cytotoxic nuclease with a staphylococcal nuclease region essential for nuclear translocation and apoptosis induction in chicken cells","volume":"99","author":"Xu","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113525004444?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113525004444?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T14:03:48Z","timestamp":1773842628000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113525004444"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":37,"alternative-id":["S0378113525004444"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2025.110808","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Antimicrobial susceptibility and genomic determinants of resistance and virulence in Mycoplasma cynos and Mycoplasma felis","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2025.110808","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"110808"}}