{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:48:50Z","timestamp":1792068530808,"version":"4.4.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100015335","name":"Modern Agricultural Technology Industry System of Shandong province","doi-asserted-by":"publisher","award":["HBCT2024260205"],"award-info":[{"award-number":["HBCT2024260205"]}],"id":[{"id":"10.13039\/501100015335","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008047","name":"Hebei University","doi-asserted-by":"publisher","award":["SJ2601001199"],"award-info":[{"award-number":["SJ2601001199"]}],"id":[{"id":"10.13039\/501100008047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Microbiology"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.vetmic.2026.111189","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T15:07:49Z","timestamp":1786374469000},"page":"111189","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Molecular characterization and biological characteristics of a highly pathogenic recombinant ALV-J strain (HUE2023) with cross-clade gp85 recombination"],"prefix":"10.1016","volume":"321","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2943-7411","authenticated-orcid":false,"given":"Chao","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziqing","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwen","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanmei","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruihan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifeng","family":"Shan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengting","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyi","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinpeng","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2490-0807","authenticated-orcid":false,"given":"Yongxiang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetmic.2026.111189_bib1","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1111\/jam.15608","article-title":"Clarification of large-volume bacterial cultures using a centrifuge-free protocol","volume":"133","author":"Allahghadry","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.vetmic.2026.111189_bib2","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1111\/j.1558-5646.2012.01803.x","article-title":"Vaccination and reduced cohort duration can drive virulence evolution: marek's disease virus and industrialized agriculture","volume":"67","author":"Atkins","year":"2013","journal-title":"Evol. Int. J. Org. Evol."},{"key":"10.1016\/j.vetmic.2026.111189_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2022.102116","article-title":"Isolation, identification, and pathogenicity of a ALV-K strain from chinese indigenous chicken breed","volume":"101","author":"Chen","year":"2022","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetmic.2026.111189_bib4","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01616-25","article-title":"MARCH2 inhibits avian leukosis virus replication by targeting gp85 for ubiquitination and degradation","volume":"99","author":"Chen","year":"2025","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2026.111189_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2020.614854","article-title":"Isolation and identification of subgroup J avian leukosis virus inducing multiple systemic tumors in parental meat-type chickens","volume":"7","author":"Cui","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.vetmic.2026.111189_bib6","doi-asserted-by":"crossref","DOI":"10.1093\/ve\/veab037","article-title":"Genetic diversity of avian leukosis virus subgroup j (ALV-J): toward a unified phylogenetic classification and nomenclature system","volume":"7","author":"Deng","year":"2021","journal-title":"Virus Evol."},{"key":"10.1016\/j.vetmic.2026.111189_bib7","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.00717-22","article-title":"The emergence, diversification, and transmission of subgroup J avian leukosis virus reveals that the live chicken trade plays a critical role in the adaption and endemicity of viruses to the yellow-chickens","volume":"96","author":"Deng","year":"2022","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2026.111189_bib8","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/s12985-024-02329-7","article-title":"Immunopathological investigation and genetic evolution of avian leukosis virus subgroup-J associated with myelocytomatosis in broiler flocks in egypt","volume":"21","author":"Fotouh","year":"2024","journal-title":"Virol. J."},{"key":"10.1016\/j.vetmic.2026.111189_bib9","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1292\/jvms.13-0263","article-title":"Multicentric histiocytosis related to avian leukosis virus subgroup J (ALV-J)-infection in meat-type local chickens","volume":"76","author":"Furukawa","year":"2014","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.vetmic.2026.111189_bib10","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1128\/JCM.02200-13","article-title":"Development and application of a multiplex PCR method for rapid differential detection of subgroup a, b, and J avian leukosis viruses","volume":"52","author":"Gao","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetmic.2026.111189_bib11","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2023.1228109","article-title":"The phylogenetic analysis of the new emerging ALV-k revealing the co-prevailing of multiple clades in chickens and a proposal for the classification of ALV-k","volume":"10","author":"Guo","year":"2023","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.vetmic.2026.111189_bib12","first-page":"7","article-title":"Isolation, Identification and Genome Sequence Analysis of Two Avian Leukemia Virus Strains of Subgroup J","volume":"57","author":"Guo","year":"2021","journal-title":"Chin. J. Vet. Med."},{"key":"10.1016\/j.vetmic.2026.111189_bib13","doi-asserted-by":"crossref","DOI":"10.1002\/vms3.70044","article-title":"Molecular detection and genetic characterization of avian leukosis virus from field outbreaks in bangladesh","volume":"10","author":"Hossain","year":"2024","journal-title":"Vet. Med. Sci."},{"key":"10.1016\/j.vetmic.2026.111189_bib14","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.vetmic.2026.111189_bib15","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.3390\/v7031020","article-title":"Myxoma virus and the leporipoxviruses: an evolutionary paradigm","volume":"7","author":"Kerr","year":"2015","journal-title":"Virus -Basel"},{"key":"10.1016\/j.vetmic.2026.111189_bib16","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.vetmic.2026.111189_bib17","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.3382\/ps\/pew407","article-title":"Gp85 genetic diversity of avian leukosis virus subgroup j among different individual chickens from a native flock","volume":"96","author":"Li","year":"2017","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetmic.2026.111189_bib18","doi-asserted-by":"crossref","first-page":"2717","DOI":"10.1007\/s00705-016-2965-x","article-title":"Isolation, identification and evolution analysis of a novel subgroup of avian leukosis virus isolated from a local chinese yellow broiler in south china","volume":"161","author":"Li","year":"2016","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetmic.2026.111189_bib19","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/s00705-024-06083-7","article-title":"Recombination of variable and host range regions of glycoprotein gp85 in different avian leukosis virus subgroup K isolates","volume":"169","author":"Li","year":"2024","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetmic.2026.111189_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2022.101764","article-title":"Whole-genome analysis reveals possible sources of ALV-J infection in an anyi tile-like gray chicken flock","volume":"101","author":"Li","year":"2022","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetmic.2026.111189_bib21","doi-asserted-by":"crossref","first-page":"152","DOI":"10.3390\/vetsci13020152","article-title":"Research progress on pathogenesis and prevention of avian leukosis virus j subgroup (ALV-J)","volume":"13","author":"Liu","year":"2026","journal-title":"Vet. Sci."},{"key":"10.1016\/j.vetmic.2026.111189_bib22","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.vetmic.2019.03.021","article-title":"A recombination efficiently increases the pathogenesis of the novel k subgroup of avian leukosis virus","volume":"231","author":"Lv","year":"2019","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2026.111189_bib23","doi-asserted-by":"crossref","first-page":"5286","DOI":"10.1016\/j.psj.2020.08.004","article-title":"Molecular characterization of avian leukosis virus subgroup j in chinese local chickens between 2013 and 2018","volume":"99","author":"Ma","year":"2020","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetmic.2026.111189_bib24","doi-asserted-by":"crossref","first-page":"vev003","DOI":"10.1093\/ve\/vev003","article-title":"RDP4: detection and analysis of recombination patterns in virus genomes","volume":"1","author":"Martin","year":"2015","journal-title":"Virus Evol."},{"key":"10.1016\/j.vetmic.2026.111189_bib25","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.vetmic.2011.10.019","article-title":"Genetic diversity and phylogenetic analysis of glycoprotein GP85 of ALV-J isolates from mainland china between 1999 and 2010: coexistence of two extremely different subgroups in layers","volume":"156","author":"Pan","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.vetmic.2026.111189_bib26","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1136\/vr.129.20.447","article-title":"Induction of myeloid leukosis and other tumours with the HPRS-103 strain of ALV","volume":"129","author":"Payne","year":"1991","journal-title":"Vet. Rec."},{"key":"10.1016\/j.vetmic.2026.111189_bib27","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1080\/03079457.2011.646237","article-title":"The long view: 40 years of avian leukosis research","volume":"41","author":"Payne","year":"2012","journal-title":"Avian Pathol. J. W. V. P. A"},{"key":"10.1016\/j.vetmic.2026.111189_bib28","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1002198","article-title":"Imperfect vaccination can enhance the transmission of highly virulent pathogens","volume":"13","author":"Read","year":"2015","journal-title":"Plos. Biol."},{"key":"10.1016\/j.vetmic.2026.111189_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.104009","article-title":"Current knowledge on the epidemiology and prevention of avian leukosis virus in china","volume":"103","author":"Tan","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetmic.2026.111189_bib30","doi-asserted-by":"crossref","first-page":"5866","DOI":"10.3390\/cimb46060350","article-title":"Identification of a new B-cell epitope on the capsid protein of avian leukosis virus and its application","volume":"46","author":"Wang","year":"2024","journal-title":"Curr. Issues Mol. Biol."},{"key":"10.1016\/j.vetmic.2026.111189_bib31","article-title":"Isolation and molecular characteristic of subgroup J avian leukosis virus in guangxi and jiangsu provinces of china during 2022-2023","volume":"104","author":"Wang","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetmic.2026.111189_bib32","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1014110","article-title":"Mapping the mutational landscape of an avian retrovirus envelope protein across its evolutionary trajectory","volume":"22","author":"Xu","year":"2026","journal-title":"PLoS. Pathog."},{"key":"10.1016\/j.vetmic.2026.111189_bib33","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01549-21","article-title":"Glycosylation of ALV-J envelope protein at sites 17 and 193 is pivotal in the virus infection","volume":"96","author":"Xu","year":"2022","journal-title":"J. Virol."},{"key":"10.1016\/j.vetmic.2026.111189_bib34","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1011928","article-title":"N123i mutation in the ALV-J receptor-binding domain region enhances viral replication ability by increasing the binding affinity with chNHE1","volume":"20","author":"Yu","year":"2024","journal-title":"PLoS. Pathog."},{"key":"10.1016\/j.vetmic.2026.111189_bib35","doi-asserted-by":"crossref","first-page":"2772","DOI":"10.3382\/ps\/pez065","article-title":"Difference in pathogenicity of 2 strains of avian leukosis virus subgroup J in broiler chicken","volume":"98","author":"Zhang","year":"2019","journal-title":"Poult. Sci."}],"container-title":["Veterinary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113526003263?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378113526003263?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:03:25Z","timestamp":1792065805000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378113526003263"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":35,"alternative-id":["S0378113526003263"],"URL":"https:\/\/doi.org\/10.1016\/j.vetmic.2026.111189","relation":{},"ISSN":["0378-1135"],"issn-type":[{"value":"0378-1135","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular characterization and biological characteristics of a highly pathogenic recombinant ALV-J strain (HUE2023) with cross-clade gp85 recombination","name":"articletitle","label":"Article Title"},{"value":"Veterinary Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetmic.2026.111189","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"111189"}}