{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T13:29:17Z","timestamp":1772803757959,"version":"3.50.1"},"reference-count":27,"publisher":"Elsevier BV","issue":"2-3","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2004,1]]},"DOI":"10.1016\/j.vetpar.2003.11.005","type":"journal-article","created":{"date-parts":[[2004,1,15]],"date-time":"2004-01-15T05:21:22Z","timestamp":1074144082000},"page":"137-145","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["The use of metal chelate affinity chromatography on the isolation of Leishmania chagasi promastigote hydrophobic proteinases"],"prefix":"10.1016","volume":"119","author":[{"given":"J.L","family":"Alves","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.W","family":"Mendon\u00e7a-Lima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.R","family":"Alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2003.11.005_BIB1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1111\/j.1574-6968.2000.tb09115.x","article-title":"Detection of cysteine-proteinases in Leishmania amazonensis promastigotes using a cross-reactive antiserum","volume":"186","author":"Alves","year":"2000","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB2","first-page":"167","article-title":"Heterogeneity of cysteine proteinases in Leishmania braziliensis and Leishmania major","volume":"26","author":"Alves","year":"1993","journal-title":"Braz. J. Med. Biol. Res."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB3","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/0169-4758(90)90350-D","article-title":"Evasion strategies of Leishmania parasites","volume":"6","author":"Bogdan","year":"1990","journal-title":"Parasitol. Today"},{"key":"10.1016\/j.vetpar.2003.11.005_BIB4","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.1016\/S0021-9258(19)69848-0","article-title":"Phase separation of intramembrane proteins in Triton X-114 solution","volume":"256","author":"Bordier","year":"1981","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB5","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0166-6851(89)90155-2","article-title":"Characterization of the promastigotes surface proteinase of Leishmania as a membrane-bound zinc endopeptidase","volume":"37","author":"Bouvier","year":"1989","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB6","doi-asserted-by":"crossref","first-page":"3102","DOI":"10.4049\/jimmunol.155.6.3102","article-title":"Role of the Leishmania surface protease gp63 in complement fixation, cell adhesion, and resistance to complement-mediated lyses","volume":"155","author":"Brittingham","year":"1995","journal-title":"J. Immunol."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB7","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0166-6851(00)00346-7","article-title":"Regulation of GP63 mRNA stability in promastigotes of virulent and attenuated Leishmania chagasi","volume":"112","author":"Brittingham","year":"2001","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB8","unstructured":"Chang, K.P., 1993. Cell biology of Leishmania. In: Davi, J.W. (Ed.), Modern Parasite Biology\u2014Cellular, Immunological, and Molecular Aspects. W.H. Freeman and Company, New York, pp. 79\u201391."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB9","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/0166-6851(88)90023-0","article-title":"Acid proteinase activity of a major surface membrane glycoprotein (gp63) from Leishmania mexicana promastigotes","volume":"27","author":"Chaudhuri","year":"1988","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB10","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1016\/0035-9203(82)90236-X","article-title":"Proteinase inhibitors as anti-leishmanial agents","volume":"76","author":"Coombs","year":"1982","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB11","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/0003-2697(80)90338-3","article-title":"Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates","volume":"102","author":"Heussen","year":"1980","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB12","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0014-5793(93)81049-6","article-title":"The lysosomal gp63-related protein in Leishmania mexicana amastigotes is a soluble metalloproteinase with an acidic pH optimum","volume":"327","author":"Ilg","year":"1993","journal-title":"FEBS Lett."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB13","doi-asserted-by":"crossref","first-page":"299","DOI":"10.4049\/jimmunol.138.1.299","article-title":"Infectivity of Leishmania braziliensis promastigotes is dependent on the increasing expression of 65,000-Dalton surface antigen","volume":"138","author":"Kweider","year":"1987","journal-title":"J. Immunol."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB14","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.vetpar.2003.11.005_BIB15","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB16","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0166-6851(98)00112-1","article-title":"Regulatory sequences and a novel gene in the msp (GP63) gene cluster of Leishmania chagasi","volume":"95","author":"McCoy","year":"1998","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB17","first-page":"25","article-title":"Evolution and expression of the Leishmania surface proteinase (gp63) gene locus","volume":"2","author":"Medina-Acosta","year":"1993","journal-title":"Infect. Agents Dis."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB18","unstructured":"MS\/FNS (Minist\u00e9rio da Sa\u00fade Funda\u00e7\u00e3o Nacional de Sa\u00fade), 1999. Informe epidemiol\u00f3gico do SUS. 7, 86\u201387."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB19","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1126\/science.4035350","article-title":"Enhanced metabolism of Leishmania donovani amastigotes at acid pH: an adaptation for intracellular growth","volume":"229","author":"Mukkada","year":"1985","journal-title":"Science"},{"key":"10.1016\/j.vetpar.2003.11.005_BIB20","unstructured":"Peters, W., Killick-Kendrik, R., 1987. The Leishmaniasis in Biology and Medicine, vol. 1. Academic Press, London, p. xiv."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB21","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1038\/258598a0","article-title":"Metal chelate affinity chromatography: a new approach to protein fractionation","volume":"258","author":"Porath","year":"1975","journal-title":"Nature"},{"key":"10.1016\/j.vetpar.2003.11.005_BIB22","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1016\/S0021-9258(18)46030-9","article-title":"Three distinct RNAs for the surface protease gp63 are differentially expressed during development of Leishmania donovani chagasi promastigotes to an infectious form","volume":"267","author":"Ramamoorthy","year":"1992","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB23","doi-asserted-by":"crossref","first-page":"8884","DOI":"10.1074\/jbc.270.15.8884","article-title":"Developmentally regulated expression of a novel 59-kDa product of the major surface protease (Msp or gp63) gene family of a Leishmania chagasi","volume":"270","author":"Roberts","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB24","doi-asserted-by":"crossref","first-page":"2613","DOI":"10.4049\/jimmunol.136.7.2613","article-title":"The involvement of the major glycoprotein (gp63) of Leishmania promastigotes in attachment to macrophages","volume":"136","author":"Russell","year":"1986","journal-title":"J. Immunol."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB25","doi-asserted-by":"crossref","first-page":"5129","DOI":"10.1128\/iai.64.12.5129-5137.1996","article-title":"Surface Zn-proteinase as a molecule for defense of Leishmania mexicana amazonensis promastigotes against cytolysis inside macrophage phagolysosomes","volume":"64","author":"Seay","year":"1996","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB26","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/0166-6851(91)90150-5","article-title":"Substrate-dependent pH optima of gp63 purified from seven strains of Leishmania","volume":"47","author":"Tzinia","year":"1991","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2003.11.005_BIB27","unstructured":"WHO (World Health Organization), 1990. Technical Report Series 793. Expert committee on the control of the leishmaniases, Geneva."}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401703004631?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401703004631?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T01:26:55Z","timestamp":1759627615000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401703004631"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":27,"journal-issue":{"issue":"2-3","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["S0304401703004631"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2003.11.005","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The use of metal chelate affinity chromatography on the isolation of Leishmania chagasi promastigote hydrophobic proteinases","name":"articletitle","label":"Article Title"},{"value":"Veterinary Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetpar.2003.11.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"converted-article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2003 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}