{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T09:59:27Z","timestamp":1787651967727,"version":"build-2736575974"},"reference-count":21,"publisher":"Elsevier BV","issue":"3-4","license":[{"start":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:00:00Z","timestamp":1209600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2008,5]]},"DOI":"10.1016\/j.vetpar.2008.01.037","type":"journal-article","created":{"date-parts":[[2008,3,3]],"date-time":"2008-03-03T20:35:06Z","timestamp":1204576506000},"page":"225-230","source":"Crossref","is-referenced-by-count":38,"title":["Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method"],"prefix":"10.1016","volume":"153","author":[{"given":"Tiago M.","family":"Martins","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ol\u00edvia C.","family":"Pedro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rubina A.","family":"Caldeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virg\u00edlio E.","family":"do Ros\u00e1rio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds","family":"Neves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Domingos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2008.01.037_bib1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1023\/B:TROP.0000048513.80797.97","article-title":"Serological survey of Babesia bovis and Anaplasma marginale in cattle in Tete Province, Mozambique","volume":"37","author":"Alfredo","year":"2005","journal-title":"Trop. Anim. Health Prod."},{"key":"10.1016\/j.vetpar.2008.01.037_bib2","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S0304-4017(01)00464-2","article-title":"Bovine piroplasms in Minorca (Balearic Islands, Spain): a comparison of PCR-based and light microscopy detection","volume":"99","author":"Almeria","year":"2001","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib3","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.vetpar.2004.05.024","article-title":"Molecular and phylogenetic characterization of Theileria spp. parasites in autochthonous bovines (Mirandesa breed) in Portugal","volume":"123","author":"Br\u00edgido","year":"2004","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib4","doi-asserted-by":"crossref","first-page":"2748","DOI":"10.1128\/JCM.34.11.2748-2755.1996","article-title":"Monitoring Babesia bovis infections in cattle by using PCR-based tests","volume":"34","author":"Calder","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib5","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1046\/j.1365-3156.2003.01074.x","article-title":"A fatal case of human babesiosis in Portugal: molecular and phylogenetic analysis","volume":"8","author":"Centeno-Lima","year":"2003","journal-title":"Trop. Med. Int. Health"},{"key":"10.1016\/j.vetpar.2008.01.037_bib6","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.vetpar.2006.02.034","article-title":"Comparison of different direct diagnostic methods to identify Babesia bovis and Babesia bigemina in animals vaccinated with live attenuated parasites","volume":"139","author":"Costa-J\u00fanior","year":"2006","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib7","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.vetpar.2006.01.040","article-title":"Expressed sequence tag (EST) analysis of the erythrocytic stages of Babesia bovis","volume":"138","author":"de Vries","year":"2006","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib8","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0304-4017(84)90063-3","article-title":"The presence of kinetes of a Babesia species in the haemolymph smears of engorged hyalomma ticks in Nigeria","volume":"17","author":"Dipeolu","year":"1984","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib9","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0304-4017(93)90008-B","article-title":"Multiplex polymerase chain reaction based assay for the detection of Babesia bigemina, Babesia bovis and Anaplasma marginale DNA in bovine blood","volume":"50","author":"Figueroa","year":"1993","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib10","first-page":"197","article-title":"PCR-based detection of the transovarial transmission of Uruguayan Babesia bovis and Babesia bigemina vaccine strains","volume":"70","author":"Gayo","year":"2003","journal-title":"Onderstepoort J. Vet. Res."},{"key":"10.1016\/j.vetpar.2008.01.037_bib11","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0304-4017(01)00488-5","article-title":"Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily","volume":"99","author":"Georges","year":"2001","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib12","first-page":"68","article-title":"GeneFisher\u2014software support for the detection of postulated genes","volume":"4","author":"Giegerich","year":"1996","journal-title":"Proc. Int. Conf. Intell. Syst. Mol. Biol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib13","first-page":"215","article-title":"Studies on a bovine Babesia transmitted by Hyalomma marginatum rufipes Koch, 1844","volume":"48","author":"Gray","year":"1981","journal-title":"Onderstepoort J. Vet. Res."},{"key":"10.1016\/j.vetpar.2008.01.037_bib14","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1128\/JCM.37.6.1782-1789.1999","article-title":"Simultaneous detection of bovine Theileria and Babesia species by reverse line blot hybridization","volume":"37","author":"Gubbels","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib15","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.mimet.2007.09.019","article-title":"Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of bovine Babesia parasites","volume":"71","author":"Iseki","year":"2007","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.vetpar.2008.01.037_bib16","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.vetpar.2005.03.007","article-title":"Babesia spp. infection in Boophilus microplus engorged females and eggs in Sao Paulo State, Brazil","volume":"130","author":"Oliveira","year":"2005","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib17","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.ijpara.2004.09.002","article-title":"PCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle","volume":"35","author":"Oliveira-Sequeira","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib18","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/j.ijpara.2003.12.012","article-title":"Application of a reverse line blot assay to the study of haemoparasites in cattle in Uganda","volume":"34","author":"Oura","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib19","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetpar.2006.01.035","article-title":"Babesia\u2014a historical overview","volume":"138","author":"Uilenberg","year":"2006","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.01.037_bib20","first-page":"259","article-title":"In vitro cultivation of Babesia occultans","volume":"63","author":"Van Niekerk","year":"1996","journal-title":"Onderstepoort J. Vet. Res."},{"key":"10.1016\/j.vetpar.2008.01.037_bib21","doi-asserted-by":"crossref","first-page":"3543","DOI":"10.1093\/nar\/6.11.3543","article-title":"Hybridization of synthetic oligodeoxyribonucleotides to PhiX 174 DNA: the effect of single base pair mismatch","volume":"6","author":"Wallace","year":"1979","journal-title":"Nucleic Acids Res."}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401708000794?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401708000794?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,2]],"date-time":"2020-05-02T15:09:07Z","timestamp":1588432147000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401708000794"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5]]},"references-count":21,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2008,5]]}},"alternative-id":["S0304401708000794"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2008.01.037","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2008,5]]}}}