{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:10:26Z","timestamp":1790525426717,"version":"4.1.0"},"reference-count":53,"publisher":"Elsevier BV","issue":"3-4","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2008,7]]},"DOI":"10.1016\/j.vetpar.2008.03.017","type":"journal-article","created":{"date-parts":[[2008,4,8]],"date-time":"2008-04-08T12:18:06Z","timestamp":1207657086000},"page":"177-184","source":"Crossref","is-referenced-by-count":62,"title":["Immunodiagnostic\/protective role of Cathepsin L cysteine proteinases secreted by Fasciola species"],"prefix":"10.1016","volume":"154","author":[{"given":"A.K.","family":"Dixit","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja","family":"Dixit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.L.","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2008.03.017_bib1","doi-asserted-by":"crossref","first-page":"6794","DOI":"10.4049\/jimmunol.161.12.6794","article-title":"Leishmania mexicana cysteine proteinase-deficient mutants have attenuated virulence for mice and potentiate a Th1 response","volume":"161","author":"Alexander","year":"1998","journal-title":"J. Immunol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib2","series-title":"Immunology of Parasitic Infections","first-page":"28","article-title":"Parasite antigens and method of analysis","author":"Anders","year":"1982"},{"key":"10.1016\/j.vetpar.2008.03.017_bib3","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1179\/000349802125000754","article-title":"Identification and characterization of a 26-28kDa circulating antigen of Fasciola gigantica","volume":"96","author":"Attalah","year":"2002","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2307\/3284308","article-title":"Proteinases secreted by Fasciola hepatica degrade extracellular matrix and basement membrane components","volume":"83","author":"Berasain","year":"1997","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib5","doi-asserted-by":"crossref","first-page":"5372","DOI":"10.1128\/IAI.67.10.5372-5378.1999","article-title":"Fasciola hepatica a protective Th1 response against Bordetella pertussis","volume":"67","author":"Brady","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetpar.2008.03.017_bib6","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0166-6851(93)90172-T","article-title":"Cathepsin L proteinase secreted by Fasciola hepatica in vitro prevents antibody mediated eosinophil attachment to newly excysted juveniles","volume":"62","author":"Carmona","year":"1993","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib7","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/0304-4017(82)90039-5","article-title":"Proteolytic cleavage of immunoglobin by enzymes released by Fasciola hepatica","volume":"11","author":"Chapman","year":"1982","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib8","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1017\/S0022149X00011627","article-title":"Antigenicity of a proteolytic enzyme of Fasciola hepatica","volume":"62","author":"Coles","year":"1988","journal-title":"J. Helminthol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib9","doi-asserted-by":"crossref","first-page":"17038","DOI":"10.1074\/jbc.M308831200","article-title":"Cathepsin L1, the major protease involved in liver fluke (Fasciola hepatica) virulence","volume":"279","author":"Collins","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetpar.2008.03.017_bib10","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/S0020-7519(99)00017-X","article-title":"Use of a preselected epitope of cathepsin L1 in a highly specific peptide based immunoassay for the diagnosis of Fasciola hepatica infections in cattle","volume":"29","author":"Cornelissen","year":"1999","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib11","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/S0020-7519(01)00175-8","article-title":"Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin L-like protease","volume":"31","author":"Cornelissen","year":"2001","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib12","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.2307\/3278170","article-title":"Isolation and labeling of Fasciola hepatica antigens active during the infection","volume":"58","author":"Cuperlovic","year":"1972","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib13","doi-asserted-by":"crossref","first-page":"5066","DOI":"10.1128\/IAI.64.12.5066-5074.1996","article-title":"Induction of protective immunity in cattle against infection with Fasciola hepatica by vaccination with cathepsin L proteinases and with hemoglobin","volume":"64","author":"Dalton","year":"1996","journal-title":"Inf. Immun."},{"key":"10.1016\/j.vetpar.2008.03.017_bib14","first-page":"5","article-title":"Purification and characterization of 28kDa cysteine proteinase for immunodiagnosis of tropical fasciolosis","volume":"17","author":"Dixit","year":"2003","journal-title":"J. Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib15","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0304-4017(02)00202-9","article-title":"28kDa Fasciola gigantica cysteine proteinase in the diagnosis of prepatent ovine fasciolosis","volume":"109","author":"Dixit","year":"2002","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib16","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1023\/B:TROP.0000009526.67899.cf","article-title":"Kinetics of antibody response using 28kDa Fasciola gigantica cysteine proteinase as antigen","volume":"36","author":"Dixit","year":"2004","journal-title":"Trop. Anim. Health Prod."},{"key":"10.1016\/j.vetpar.2008.03.017_bib17","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0166-6851(97)00090-X","article-title":"Isolation of a cDNA encoding Fasciola hepatica cathepsin L and functional expression in Saccharomyces cerevisiae","volume":"88","author":"Dowd","year":"1997","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib18","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/j.1432-1033.1995.tb20805.x","article-title":"Fasciola hepatica cathepsin L cleaves fibrinogen and produces a novel type of fibrinogen clot","volume":"232","author":"Dowd","year":"1995","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.vetpar.2008.03.017_bib19","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/j.1432-1033.1994.tb18969.x","article-title":"Purification and characterisation of a second cathepsin L proteinase secreted by the parasitic trematode Fasciola hepatica","volume":"223","author":"Dowd","year":"1994","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.vetpar.2008.03.017_bib20","unstructured":"Edith, R., 2004. Comparative immunoprotection profile of infection specific and excretory secretory proteins of Fasciola gigantica against tropical fasciolosis in buffaloes. M.V.Sc. Thesis. I.V.R.I. Deemed University, Izatnagar, pp.1\u201396."},{"key":"10.1016\/j.vetpar.2008.03.017_bib21","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1016\/S0020-7519(97)00096-9","article-title":"Evaluation of antigens of Fasciola gigantica as vaccines against tropical fasciolosis in cattle","volume":"27","author":"Estuningsih","year":"1997","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib22","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S1383-5769(01)00068-X","article-title":"Molecular cloning and characterisation of cathepsin L encoding genes from Fasciola gigantica","volume":"50","author":"Grams","year":"2001","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.vetpar.2008.03.017_bib23","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.ijpara.2003.12.011","article-title":"Identification of a novel Fasciola hepatica cathepsin L protease containing protective epitopes within the propeptide","volume":"34","author":"Harmsen","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib24","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0166-6851(94)90130-9","article-title":"Cloning of protease gene family of Fasciola hepatica by polymerase chain reaction","volume":"64","author":"Heussler","year":"1994","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib25","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/icb.1980.20","article-title":"A difference in surface proteins of Fasciola hepatica larvae from intact and nude mice","volume":"58","author":"Howard","year":"1980","journal-title":"Aus. J. Exp. Bio. Med. Sci."},{"key":"10.1016\/j.vetpar.2008.03.017_bib26","doi-asserted-by":"crossref","unstructured":"Jezek, J., Ridi, R.E., Salah, M., Wagih, A., Aziz, H.W., Tallima, H., Shafie, M.H., Khalek, T.A., Ammou, F.F., Strongylis, C., Moussis, V., Tsikaris, V., 2007. Fasciola gigantica cathepsin L proteinase-based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis. Biopolymers, in press.","DOI":"10.1002\/bip.20788"},{"key":"10.1016\/j.vetpar.2008.03.017_bib27","doi-asserted-by":"crossref","first-page":"3619","DOI":"10.1016\/j.vaccine.2007.01.057","article-title":"Enteral vaccination of rats against Fasciola hepatica using recombinant cysteine proteinase (cathepsin L1)","volume":"25","author":"Kesik","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2008.03.017_bib28","doi-asserted-by":"crossref","first-page":"2985","DOI":"10.1016\/S0264-410X(00)00095-5","article-title":"Successful DNA immunisation of rats against fasciolosis","volume":"18","author":"Kofta","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2008.03.017_bib29","doi-asserted-by":"crossref","first-page":"2972","DOI":"10.1002\/eji.1830261224","article-title":"Potential allergens stimulate the release of mediators of the allergic response from cells of mast cell lineage in the absence of sensitization with antigen-specific IgE","volume":"26","author":"Machado","year":"1996","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib30","doi-asserted-by":"crossref","first-page":"648","DOI":"10.4269\/ajtmh.1999.61.648","article-title":"Fasciola gigantica-specific antigens: purification by a continuous-elution method and its evaluation for the diagnosis of human fascioliasis","volume":"61","author":"Maleewong","year":"1999","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.vetpar.2008.03.017_bib31","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1016\/S0264-410X(97)00289-2","article-title":"Correlation of specific antibody titre and avidity with proterction in cattle immunized against Fasciola hepatica","volume":"16","author":"Mulcahy","year":"1998","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2008.03.017_bib32","series-title":"Fasciolosis","first-page":"341","article-title":"Immunology of Fasciola hepatica infection","author":"Mulcahy","year":"1999"},{"key":"10.1016\/j.vetpar.2008.03.017_bib33","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1046\/j.1365-3024.2000.00290.x","article-title":"Fasciola hepatica infection down-regulates Th1 responses in mice","volume":"22","author":"O\u2019Neill","year":"2000","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib34","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1046\/j.1365-3024.2001.00411.x","article-title":"Fasciola hepatica cathepsin L cysteine proteinase suppresses Bordetella pertussis-specific interferon-gamma production in vivo","volume":"23","author":"O\u2019Neill","year":"2001","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib35","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1128\/IAI.67.4.1954-1961.1999","article-title":"Vaccination with cathepsin l-proteinases and with leucine amino peptidase induces high levels of protection against Fascioliasis in sheep","volume":"67","author":"Piacenza","year":"1999","journal-title":"Inf. Immun."},{"key":"10.1016\/j.vetpar.2008.03.017_bib36","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1046\/j.0141-9838.2001.00438.x","article-title":"Fasciola hepatica cathepsin L suppresses sheep lymphocyte proliferation in vitro and modulates surface CD4 expression on human and ovine T cell","volume":"24","author":"Prowse","year":"2002","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib37","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.actatropica.2006.03.004","article-title":"Immunodiagnosis of bubaline fasciolosis with Fasciola gigantica Cathepsin L and recombinant Cathepsin L 1-D proteases","volume":"98","author":"Raina","year":"2006","journal-title":"Acta Trop."},{"key":"10.1016\/j.vetpar.2008.03.017_bib38","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/j.1432-1033.1997.t01-1-00373.x","article-title":"Functional expression of Fasciola hepatica cathepsin L1 in Saccharomyces cerevisiae","volume":"245","author":"Roche","year":"1997","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.vetpar.2008.03.017_bib39","doi-asserted-by":"crossref","first-page":"4894","DOI":"10.1128\/IAI.63.12.4894-4899.1995","article-title":"Disruption of the murine interleukin-4 gene inhibits disease progression during Leishmania mexicana infection but does not increase control of Leishmania donovani infection","volume":"63","author":"Satoskar","year":"1995","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetpar.2008.03.017_bib40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0166-6851(93)90171-S","article-title":"Purification of a cathepsin L like proteinase secreted by adult Fasciola hepatica","volume":"62","author":"Smith","year":"1993","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib41","doi-asserted-by":"crossref","first-page":"2567","DOI":"10.1110\/ps.9.12.2567","article-title":"A single amino acid substitution affects substrate specificity in cysteine proteinases from Fasciola hepatica","volume":"9","author":"Smooker","year":"2000","journal-title":"Protein Sci."},{"key":"10.1016\/j.vetpar.2008.03.017_bib42","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1016\/S0020-7519(97)00120-3","article-title":"Immunological approaches for the control of fasciolosis","volume":"27","author":"Spithill","year":"1997","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib43","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.vetpar.2005.10.016","article-title":"Cathepsin L cysteine proteinase in the diagnosis of bovine Fasciola gigantica infection","volume":"135","author":"Sriveny","year":"2006","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib44","doi-asserted-by":"crossref","first-page":"82","DOI":"10.4269\/ajtmh.2005.72.82","article-title":"Serodiagnosis of human fascioliasis by a cystatin capture enzyme-linked immunosorbent assay with recombinant Fasciola gigantica cathepsin L antigen","volume":"72","author":"Tantrawatpan","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.vetpar.2008.03.017_bib45","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S0065-308X(08)60243-2","article-title":"Proteinases and associated genes of parasitic helminths","volume":"43","author":"Tort","year":"1999","journal-title":"Adv. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib46","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.vetpar.2007.03.023","article-title":"Vaccine potential of inclusion bodies containing cysteine proteinase of Fasciola hepatica in calves and lambs experimentally challenged with metacercariae of the fluke","volume":"147","author":"Wedrychowicz","year":"2007","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib47","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0165-2427(03)00085-0","article-title":"The immune response of rats to vaccination with the cDNA or protein forms of the cysteine proteinase of Fasciola hepatica","volume":"94","author":"Wedrychowicz","year":"2003","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib48","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1042\/bj2990781","article-title":"The secreted cathepsin L-like proteinases of the trematode, Fasciola hepatica, contain 3-hydroxyproline residues","volume":"299","author":"Wijffles","year":"1994","journal-title":"Biochem. J."},{"key":"10.1016\/j.vetpar.2008.03.017_bib49","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1006\/expr.1994.1014","article-title":"Vaccination of sheep with purified cysteine proteinases of Fasciola hepatica decreases worm fecundity","volume":"78","author":"Wijffles","year":"1994","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2008.03.017_bib50","first-page":"73","article-title":"A comparative evaluation of Fasciola gigantica antigens in immunodiagnosis of bubaline fasciolosis","volume":"16","author":"Yadav","year":"1999","journal-title":"Rivista Di Parasitologia"},{"key":"10.1016\/j.vetpar.2008.03.017_bib51","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1007\/s00436-005-1466-8","article-title":"Fasciola gigantica Cathepsin L cysteine proteinase in the detection of early experimental fasciolosis in ruminants","volume":"97","author":"Yadav","year":"2005","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2008.03.017_bib52","first-page":"537","article-title":"Cloning and sequence analysis of the major cysteine protease expressed in the trematode parasite Fasciola spp.","volume":"31","author":"Yamasaki","year":"1993","journal-title":"Biochem. Mol. Biol. Int."},{"key":"10.1016\/j.vetpar.2008.03.017_bib53","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/S0020-7519(02)00057-7","article-title":"Characterisation and expression of the Fasciola gigantica Cathepsin L gene","volume":"32","author":"Yamasaki","year":"2002","journal-title":"Int. J. Parasitol."}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401708001775?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401708001775?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T11:11:28Z","timestamp":1684321888000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401708001775"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7]]},"references-count":53,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2008,7]]}},"alternative-id":["S0304401708001775"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2008.03.017","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2008,7]]}}}