{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T14:32:01Z","timestamp":1789914721214,"version":"4.0.1"},"reference-count":35,"publisher":"Elsevier BV","issue":"2-4","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"CONICET; INTA; ANPCyT","award":["PICTR 2002-00054"],"award-info":[{"award-number":["PICTR 2002-00054"]}]},{"name":"CONICET; INTA; ANPCyT","award":["PICTO 08-12920)"],"award-info":[{"award-number":["PICTO 08-12920)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2010,2]]},"DOI":"10.1016\/j.vetpar.2009.09.023","type":"journal-article","created":{"date-parts":[[2009,9,21]],"date-time":"2009-09-21T08:09:32Z","timestamp":1253520572000},"page":"216-226","source":"Crossref","is-referenced-by-count":26,"title":["In silico predicted conserved B-cell epitopes in the merozoite surface antigen-2 family of B. bovis are neutralization sensitive"],"prefix":"10.1016","volume":"167","author":[{"given":"M.","family":"Dominguez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Echaide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. Torioni de","family":"Echaide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Mosqueda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Cetr\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.E.","family":"Suarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Florin-Christensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2009.09.023_bib1","first-page":"47","article-title":"Protecci\u00f3n conferida por Babesia bovis vacunal en novillos Holando Argentino","volume":"74","author":"Anziani","year":"1993","journal-title":"Rev. Med. Vet."},{"key":"10.1016\/j.vetpar.2009.09.023_bib2","doi-asserted-by":"crossref","first-page":"7180","DOI":"10.1128\/IAI.73.11.7180-7189.2005","article-title":"Merozoite Surface Antigen 2 proteins of Babesia bovis vaccine breakthrough isolates contain a unique hypervariable region composed of degenerate repeats","author":"Berens","year":"2005","journal-title":"Infect. Immun."},{"issue":"1\u20132","key":"10.1016\/j.vetpar.2009.09.023_bib3","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0304-4017(92)90047-D","article-title":"Investigations of breakdowns in protection provided by living Babesia bovis vaccine","volume":"43","author":"Bock","year":"1992","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2009.09.023_bib4","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1111\/j.1751-0813.1995.tb03558.x","article-title":"Studies on failure of T strain live Babesia bovis vaccine","volume":"72","author":"Bock","year":"1995","journal-title":"Aust. Vet. J."},{"key":"10.1016\/j.vetpar.2009.09.023_bib5","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1371\/journal.ppat.0030148","article-title":"Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoa","volume":"3","author":"Brayton","year":"2007","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.vetpar.2009.09.023_bib6","doi-asserted-by":"crossref","first-page":"686","DOI":"10.4269\/ajtmh.2005.73.686","article-title":"Preliminary observations on the efficacy of a recombinant multistage Plasmodium falciparum vaccine in Aotus nancymai monkeys","volume":"73","author":"Collins","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"2","key":"10.1016\/j.vetpar.2009.09.023_bib7","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/0092-8674(84)90397-0","article-title":"Genes of the protozoan parasite Babesia bovis that rearrange to produce RNA species with different sequences","volume":"37","author":"Cowman","year":"1984","journal-title":"Cell"},{"key":"10.1016\/j.vetpar.2009.09.023_bib8","first-page":"254","article-title":"Evaluaci\u00f3n de la din\u00e1mica de la infecci\u00f3n por Babesia bovis y Babesia bigemina en terneros. Diagnostico por microscopia directa y prueba de inmunofluorescencia indirecta","volume":"69","author":"de R\u00edos","year":"1988","journal-title":"Rev. Med. Vet."},{"key":"10.1016\/j.vetpar.2009.09.023_bib9","series-title":"Buenos Aires, Argentina, Nov. 15\u201317, 2004","first-page":"102","article-title":"Evaluaci\u00f3n de ant\u00edgenos de Babesia bovis en un ELISA indirecto","author":"Echaide","year":"2004"},{"issue":"7","key":"10.1016\/j.vetpar.2009.09.023_bib10","doi-asserted-by":"crossref","first-page":"3566","DOI":"10.1128\/IAI.70.7.3566-3575.2002","article-title":"The Babesia bovis merozoite surface antigen 2 locus contains four tandemly arranged and expressed genes encoding immunologically distinct proteins","volume":"70","author":"Florin-Christensen","year":"2002","journal-title":"Infect. Immun."},{"issue":"9","key":"10.1016\/j.vetpar.2009.09.023_bib11","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.1128\/IAI.56.9.2363-2368.1988","article-title":"Identification of Babesia bovis merozoite surface antigens by using immune bovine sera and monoclonal antibodies","volume":"56","author":"Goff","year":"1988","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetpar.2009.09.023_bib12","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1017\/S0031182006001399","article-title":"Recombinant protein Bd37 protected gerbils against heterologous challenges with isolates of Babesia divergens polymorphic for the bd37 gene","volume":"134","author":"Hadj-Kaddour","year":"2007","journal-title":"Parasitology"},{"key":"10.1016\/j.vetpar.2009.09.023_bib13","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"issue":"1","key":"10.1016\/j.vetpar.2009.09.023_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0166-6851(89)90096-0","article-title":"Molecular characterization of Babesia bovis merozoite surface proteins bearing epitopes immunodominant in protected cattle","volume":"37","author":"Hines","year":"1989","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2009.09.023_bib15","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/0166-6851(92)90129-8","article-title":"Neutralization-sensitive merozoite surface antigens of Babesia bovis encoded by members of a polymorphic gene family","volume":"55","author":"Hines","year":"1992","journal-title":"Mol. Biochem. Parasitol."},{"issue":"1","key":"10.1016\/j.vetpar.2009.09.023_bib16","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1128\/IAI.63.1.349-352.1995","article-title":"Immunization of cattle with recombinant Babesia bovis merozoite surface antigen-1","volume":"63","author":"Hines","year":"1995","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetpar.2009.09.023_bib17","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1111\/j.1365-3024.2005.00795.x","article-title":"Potential for recombinant Babesia bovis antigens to protect against a highly virulent isolate","volume":"27","author":"Hope","year":"2005","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.vetpar.2009.09.023_bib18","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/0166-6851(92)90128-7","article-title":"Surface epitope localization and gene structure of Babesia bovis 44-kilodalton variable merozoite surface antigen","volume":"55","author":"Jasmer","year":"1992","journal-title":"Mol. Biochem. Parasitol."},{"issue":"1\u20132","key":"10.1016\/j.vetpar.2009.09.023_bib19","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/0014-5793(90)80535-Q","article-title":"A semi-empirical method for prediction of antigenic determinants on protein antigens","volume":"276","author":"Kolaskar","year":"1990","journal-title":"FEBS Lett."},{"key":"10.1016\/j.vetpar.2009.09.023_bib20","doi-asserted-by":"crossref","first-page":"3663","DOI":"10.1128\/IAI.00032-06","article-title":"The Babesia bovis merozoite surface antigen 1 hypervariable region induces surface-reactive antibodies that block merozoite invasion","volume":"74","author":"LeRoith","year":"2006","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetpar.2009.09.023_bib21","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1126\/science.7355284","article-title":"Babesia bovis: continuous cultivation in a microaerophilous stationary phase culture","volume":"207","author":"Levy","year":"1980","journal-title":"Science"},{"key":"10.1016\/j.vetpar.2009.09.023_bib22","doi-asserted-by":"crossref","first-page":"6448","DOI":"10.1128\/IAI.70.11.6448-6455.2002","article-title":"Babesia bovis merozoite surface antigen 2 proteins are expressed on the merozoite and sporozoite surface, and specific antibodies inhibit attachment and invasion of erythrocytes","volume":"70","author":"Mosqueda","year":"2002","journal-title":"Infect. Immun."},{"issue":"23","key":"10.1016\/j.vetpar.2009.09.023_bib23","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1016\/j.vaccine.2004.09.039","article-title":"Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge","volume":"23","author":"Musoke","year":"2005","journal-title":"Vaccine"},{"issue":"9","key":"10.1016\/j.vetpar.2009.09.023_bib24","doi-asserted-by":"crossref","first-page":"5021","DOI":"10.1128\/IAI.71.9.5021-5032.2003","volume":"71","author":"Norimine","year":"2003","journal-title":"Infect. Immun."},{"issue":"9","key":"10.1016\/j.vetpar.2009.09.023_bib25","doi-asserted-by":"crossref","first-page":"3340","DOI":"10.1128\/IAI.59.9.3340-3342.1991","article-title":"Strain variation of Babesia bovis merozoite surface-exposed epitopes","volume":"59","author":"Palmer","year":"1991","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetpar.2009.09.023_bib26","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.pep.2008.05.018","article-title":"Development of pilot scale production process and characterization of a recombinant multiepitope malarial vaccine candidate FALVAC-1A expressed in Escherichia coli","volume":"61","author":"Ravi","year":"2008","journal-title":"Protein Expr. Purif."},{"issue":"3","key":"10.1016\/j.vetpar.2009.09.023_bib27","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/0166-6851(89)90210-7","article-title":"A recombinant surface protein of Babesia bovis elicits bovine antibodies that react with live merozoites","volume":"35","author":"Reduker","year":"1989","journal-title":"Mol. Biochem. Parasitol."},{"issue":"2","key":"10.1016\/j.vetpar.2009.09.023_bib28","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/0014-4894(86)90157-8","article-title":"Babesia bovis: purification and concentration of merozoites and infected bovine erythrocytes","volume":"61","author":"Rodriguez","year":"1986","journal-title":"Exp. Parasitol."},{"issue":"1","key":"10.1016\/j.vetpar.2009.09.023_bib29","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0020-7519(99)00161-7","article-title":"A Common high molecular weight antigen of Babesia bovis isolates from Mexico","volume":"30","author":"Sahagun Ruiz","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2009.09.023_bib30","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1073\/pnas.96.4.1615","article-title":"Immunogenicity and in vitro protective efficacy of a recombinant multistage Plasmodium falciparum candidate vaccine","volume":"96","author":"Shi","year":"1999","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.vetpar.2009.09.023_bib31","doi-asserted-by":"crossref","first-page":"2902","DOI":"10.1016\/S0264-410X(00)00045-1","article-title":"Development, expression, and murine testing of a multistage Plasmodium falciparum malaria vaccine candidate","volume":"18","author":"Shi","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2009.09.023_bib32","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.pt.2007.07.003","article-title":"Attenuated vaccines for tropical theileriosis, babesiosis and heartwater: the continuing necessity","volume":"23","author":"Shkap","year":"2007","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.vetpar.2009.09.023_bib33","doi-asserted-by":"crossref","first-page":"6865","DOI":"10.1128\/IAI.68.12.6865-6870.2000","article-title":"Characterization of allelic variation in the Babesia bovis merozoite surface antigen 1 (MSA-1) locus and identification of a cross-reactive inhibition-sensitive MSA-1 epitope","volume":"68","author":"Suarez","year":"2000","journal-title":"Infect. Immun."},{"issue":"2","key":"10.1016\/j.vetpar.2009.09.023_bib34","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0166-6851(02)00329-8","article-title":"Babesia bovis merozoite surface protein-2c (MSA-2c) contains highly immunogenic, conserved B-cell epitopes that elicit neutralization-sensitive antibodies in cattle","volume":"127","author":"Wilkowsky","year":"2003","journal-title":"Mol. Biochem. Parasitol."},{"issue":"1","key":"10.1016\/j.vetpar.2009.09.023_bib35","doi-asserted-by":"crossref","first-page":"14","DOI":"10.4161\/hv.2.1.2437","article-title":"Development, characterization and immunogenicity of a multi-stage, multi-valent Plasmodium falciparum Vaccine Antigen (FALVAC-1A) expressed in Escherichia coli","volume":"2","author":"Zhou","year":"2006","journal-title":"Human Vaccines"}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401709005561?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401709005561?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T10:14:52Z","timestamp":1590142492000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401709005561"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2]]},"references-count":35,"journal-issue":{"issue":"2-4","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["S0304401709005561"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2009.09.023","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2010,2]]}}}