{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T17:10:19Z","timestamp":1777482619296,"version":"3.51.4"},"reference-count":45,"publisher":"Elsevier BV","issue":"3-4","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Oniris"},{"name":"National Institute for Agricultural Research (INRA)"},{"DOI":"10.13039\/501100012166","name":"973 Program","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"NSFC","doi-asserted-by":"crossref","award":["30800820"],"award-info":[{"award-number":["30800820"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"NSFC","doi-asserted-by":"crossref","award":["30972182"],"award-info":[{"award-number":["30972182"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"NSFC","doi-asserted-by":"crossref","award":["31072130"],"award-info":[{"award-number":["31072130"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"NSFC","doi-asserted-by":"crossref","award":["31001061"],"award-info":[{"award-number":["31001061"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Key Project of Gansu Province"},{"name":"Beef and Yak Production System Programme"},{"name":"MOA"},{"name":"Specific Fund for Sino-Europe Cooperation, MOST, China"},{"name":"State Key Laboratory of Veterinary Etiological Biology Project"},{"name":"EPIZONE"},{"name":"ASFRISK","award":["211691"],"award-info":[{"award-number":["211691"]}]},{"name":"ARBOZOONET","award":["211757"],"award-info":[{"award-number":["211757"]}]},{"name":"European Commission, Brussels, Belgium"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2012,7]]},"DOI":"10.1016\/j.vetpar.2012.01.021","type":"journal-article","created":{"date-parts":[[2012,1,21]],"date-time":"2012-01-21T08:16:41Z","timestamp":1327133801000},"page":"421-430","source":"Crossref","is-referenced-by-count":11,"title":["BQP35 is a novel member of the intrinsically unstructured protein (IUP) family which is a potential antigen for the sero-diagnosis of Babesia sp. BQ1 (Lintan) infection"],"prefix":"10.1016","volume":"187","author":[{"given":"Guiquan","family":"Guan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuelle","family":"Moreau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junlong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miling","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne","family":"Rogniaux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aihong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingli","family":"Niu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youquan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaoyun","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianxun","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Chauvin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2012.01.021_bib0005","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.bbrc.2003.12.086","article-title":"Biophysical characterization of Gir2, a highly acidic protein of Saccharomyces cerevisiae with anomalous electrophoretic behavior","volume":"314","author":"Alves","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0010","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.bbrc.2005.04.151","article-title":"Gir2 is an intrinsically unstructured protein that is present in Saccharomyces cerevisiae as a group of heterogeneously electrophoretic migrating forms","volume":"332","author":"Alves","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0015","doi-asserted-by":"crossref","first-page":"S16","DOI":"10.1007\/s00436-001-0563-6","article-title":"Isolation and preliminary characterization of a large Babesia sp. from sheep and goats in the eastern part of Gansu Province, China","volume":"88","author":"Bai","year":"2002","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0020","doi-asserted-by":"crossref","first-page":"S247","DOI":"10.1017\/S0031182004005190","article-title":"Babesiosis of cattle","volume":"129","author":"Bock","year":"2004","journal-title":"Parasitology"},{"key":"10.1016\/j.vetpar.2012.01.021_bib0025","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.vetpar.2005.12.025","article-title":"Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbent assay","volume":"137","author":"Boonchit","year":"2006","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0030","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0304-4017(94)03111-9","article-title":"Current state and future trends in the diagnosis of babesiosis","volume":"57","author":"B\u00f6se","year":"1995","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0035","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.bbrc.2008.03.071","article-title":"The 45-kDa form of Pdx-1 does not result from post-translational modifications","volume":"370","author":"Carlotti","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0040","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1074\/mcp.M500181-MCP200","article-title":"A novel two-dimensional electrophoresis technique for the identification of intrinsically unstructured proteins","volume":"5","author":"Csizm\u00f3k","year":"2006","journal-title":"Mol. Cell. Proteom."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0045","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1021\/pr060450b","article-title":"Differential proteomic analysis of four near-isogenic Brassica napus varieties bred for their erucic acid and glucosinolate contents","volume":"6","author":"Devouge","year":"2007","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0050","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/S1093-3263(00)00138-8","article-title":"Intrinsically disordered protein","volume":"19","author":"Dunker","year":"2001","journal-title":"J. Mol. Graph. Model."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0055","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1038\/nrm1589","article-title":"Intrinsically unstructured proteins and their functions","volume":"6","author":"Dyson","year":"2005","journal-title":"Nat. Rev. Mol. Cell. Biol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0060","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.molbiopara.2006.08.011","article-title":"Abundance of intrinsically unstructured proteins in P. falciparum and other apicomplexan parasite proteomes","volume":"150","author":"Feng","year":"2006","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0065","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1017\/S0031182000061163","article-title":"Identification of common surface-antigens among Babesia bigemina isolates by using monoclonal antibodies","volume":"100","author":"Figueroa","year":"1990","journal-title":"Parasitology"},{"key":"10.1016\/j.vetpar.2012.01.021_bib0070","doi-asserted-by":"crossref","first-page":"3566","DOI":"10.1128\/IAI.70.7.3566-3575.2002","article-title":"The Babesia bovis merozoite surface antigen 2 locus contains four tandemly arranged and expressed genes encoding immunologically distinct proteins","volume":"70","author":"Florin-Christensen","year":"2002","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0075","doi-asserted-by":"crossref","unstructured":"Friedhoff, K.T., 1988. Transmission of Babesia. In: Ristic, M. (Ed.), Babesiosis of Domestic Animals and Man, 1st edition, CRC press, Boca Raton, FL, pp. 23\u201352.","DOI":"10.1201\/9781351070027-2"},{"key":"10.1016\/j.vetpar.2012.01.021_bib0080","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/s10493-007-9065-2","article-title":"Cloning and characterization of a ribosomal protein L23a from Haemaphysalis qinghaiensis eggs by immuno-screening of a cDNA expression library","volume":"41","author":"Gao","year":"2007","journal-title":"Exp. Appl. Acarol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0085","doi-asserted-by":"crossref","first-page":"3407","DOI":"10.1002\/elps.200500261","article-title":"Anomalous electrophoretic behavior of a very acidic protein: ribonuclease U2","volume":"26","author":"Garc\u00eda-Ortega","year":"2005","journal-title":"Electrophoresis"},{"key":"10.1016\/j.vetpar.2012.01.021_bib0090","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1128\/CVI.00150-08","article-title":"Validation of a competitive enzyme-linked immunosorbent assay for detection of Babesia bigemina antibodies in cattle","volume":"15","author":"Goff","year":"2008","journal-title":"Clin. Vaccine. Immunol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0095","first-page":"35","article-title":"Isolation of a large ovine Babesia sp. in Xinjiang, China","volume":"31","author":"Guan","year":"2001","journal-title":"Chin. J. Vet. Sci. Technol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0100","doi-asserted-by":"crossref","first-page":"S22","DOI":"10.1007\/s00436-001-0564-5","article-title":"Transmission of Babesia sp. to sheep with field-collected Haemaphysalis qinghaiensis","volume":"88","author":"Guan","year":"2002","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0105","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.exppara.2008.04.012","article-title":"The development and evaluation of a loop-mediated isothermal amplification (LAMP) method for detection of Babesia spp. infective to sheep and goats in China","volume":"120","author":"Guan","year":"2008","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0110","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1017\/S0031182009991922","article-title":"Merozoite proteins from Babesia sp. BQ1 (Lintan) as potential antigens for serodiagnosis by ELISA","volume":"137","author":"Guan","year":"2010","journal-title":"Parasitology"},{"key":"10.1016\/j.vetpar.2012.01.021_bib0115","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.vetpar.2010.02.006","article-title":"Determination of erythrocyte susceptibility of Chinese sheep (Tan mutton breed) and French sheep (Vend\u00e9en breed) to Babesia sp. BQ1 (Lintan) by in vitro culture","volume":"170","author":"Guan","year":"2010","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0120","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.pep.2004.02.008","article-title":"Cloning, expression, purification, and characterization of Nocardia sp. GTP cyclohydrolase I","volume":"35","author":"He","year":"2004","journal-title":"Protein. Expr. Purif."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0125","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/cmi.2008.41","article-title":"Rwdd1, a thymus aging related molecule, is a new member of the intrinsically unstructured protein family","volume":"5","author":"Kang","year":"2008","journal-title":"Cell. Mol. Immunol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0130","doi-asserted-by":"crossref","first-page":"E830","DOI":"10.1152\/ajpendo.00390.2002","article-title":"Sumoylation of Pdx1 is associated with its nuclear localization and insulin gene activation","volume":"284","author":"Kishi","year":"2003","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0135","doi-asserted-by":"crossref","unstructured":"Kuttler, K.L., 1988. World-wide impact of babesiosis. In: Ristic, M. (Ed.), Babesiosis of Domestic Animals and Man, 1st edition, CRC press, Boca Raton, FL, pp. 1\u201322.","DOI":"10.1201\/9781351070027-1"},{"key":"10.1016\/j.vetpar.2012.01.021_bib0140","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1006\/expr.1993.1068","article-title":"Plasmodium: control of gene expression in malaria parasites","volume":"77","author":"Lanzer","year":"1993","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0145","doi-asserted-by":"crossref","first-page":"5734","DOI":"10.1021\/bi00566a011","article-title":"Behavior of glycopolypeptides with empirical molecular weight estimation methods. 1. In sodium dodecyl sulfate","volume":"19","author":"Leach","year":"1980","journal-title":"Biochemistry"},{"key":"10.1016\/j.vetpar.2012.01.021_bib0150","first-page":"116","article-title":"A large Babesia sp. new-found from affected sheep in China","volume":"17","author":"Lian","year":"1997","journal-title":"Chin. J. Vet. Sci."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0155","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.vetpar.2007.03.032","article-title":"At least two genetically distinct large Babesia species infective to sheep and goats in China","volume":"147","author":"Liu","year":"2007","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0160","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1074\/jbc.274.2.1011","article-title":"Glucose stimulates translocation of the homeodomain transcription factor PDX1 from the cytoplasm to the nucleus in pancreatic beta-cells","volume":"274","author":"Macfarlane","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0165","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s004360050465","article-title":"Development of an enzyme-linked immunosorbent assay for detection of antibodies to Babesia bigemina in cattle","volume":"84","author":"Molloy","year":"1998","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0170","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/j.exer.2007.07.019","article-title":"Establishment of an appropriate animal model for lacritin studies: cloning and characterization of lacritin in monkey eyes","volume":"85","author":"Nakajima","year":"2007","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0175","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.exppara.2008.09.021","article-title":"Differentiation of two ovine Babesia based on the ribosomal DNA internal transcribed spacer (ITS) sequences","volume":"121","author":"Niu","year":"2009","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0180","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1007\/s00436-009-1344-x","article-title":"Detection and differentiation of ovine Theileria and Babesia by reverse line blotting in China","volume":"104","author":"Niu","year":"2009","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0185","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.1073\/pnas.0813167106","article-title":"Detergent binding explains anomalous SDS-PAGE migration of membrane proteins","volume":"106","author":"Rath","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0190","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"2001"},{"key":"10.1016\/j.vetpar.2012.01.021_bib0195","doi-asserted-by":"crossref","first-page":"D786","DOI":"10.1093\/nar\/gkl893","article-title":"DisProt: the database of disordered proteins","volume":"35","author":"Sickmeier","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0200","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1128\/JCM.38.6.2240-2247.2000","article-title":"Cloning, characterization, and expression of a 200-kilodalton diagnostic antigen of Babesia bigemina","volume":"38","author":"Tebele","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0205","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1002\/pmic.200800783","article-title":"OVNIp: an open source application facilitating the interpretation, the validation and the edition of proteomics data generated by MS analyses and de novo sequencing","volume":"10","author":"Tessier","year":"2010","journal-title":"Proteomics"},{"key":"10.1016\/j.vetpar.2012.01.021_bib0210","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S0968-0004(02)02169-2","article-title":"Intrinsically unstructured proteins","volume":"27","author":"Tompa","year":"2002","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0215","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetpar.2006.01.035","article-title":"Babesia\u2014a historical overview","volume":"138","author":"Uilenberg","year":"2006","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0220","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/0304-4017(86)90091-9","article-title":"Species-specific serodiagnosis of equine piroplasma infections by means of complement fixation test (CFT), immunofluorescence (IIF), and enzyme-linked immunosorbent assay (ELISA)","volume":"20","author":"Weiland","year":"1986","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2012.01.021_bib0225","first-page":"7","article-title":"Isolation and morphological observation of Babesia motasi and Babesia ovis in China","volume":"10","author":"Yin","year":"1997","journal-title":"Chin. J. Vet. Sci. Technol."}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401712000404?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401712000404?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:30:31Z","timestamp":1593563431000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401712000404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7]]},"references-count":45,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2012,7]]}},"alternative-id":["S0304401712000404"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2012.01.021","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2012,7]]}}}