{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:41:27Z","timestamp":1791132087115,"version":"4.1.0"},"reference-count":36,"publisher":"Elsevier BV","issue":"3-4","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,8,18]],"date-time":"2015-08-18T00:00:00Z","timestamp":1439856000000},"content-version":"vor","delay-in-days":382,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Entomologia Molecular"},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004809","name":"FINEP","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004263","name":"FAPERGS","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2014,8]]},"DOI":"10.1016\/j.vetpar.2014.05.042","type":"journal-article","created":{"date-parts":[[2014,6,7]],"date-time":"2014-06-07T18:00:13Z","timestamp":1402164013000},"page":"316-322","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"title":["In vitro establishment of ivermectin-resistant Rhipicephalus microplus cell line and the contribution of ABC transporters on the resistance mechanism"],"prefix":"10.1016","volume":"204","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8569-8150","authenticated-orcid":false,"given":"Paula C.","family":"Pohl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle D.","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirlei","family":"Daffre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0309-9328","authenticated-orcid":false,"given":"Itabajara","family":"da Silva Vaz Jr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aoi","family":"Masuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2014.05.042_bib0005","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.1120698109","article-title":"Single amino acid mutation in an ATP-binding cassette transporter gene causes resistance to Bt toxin Cry1Ab in the silkworm, Bombyx mori","volume":"109","author":"Atsumi","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0010","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.vetpar.2010.12.035","article-title":"Determination of the susceptibility of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) to ivermectin and fipronil by Larval Immersion Test (LIT) in Uruguay","volume":"178","author":"Castro-Janer","year":"2011","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0015","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1603\/0022-2585-39.2.278","article-title":"In vitro generation of organophosphate resistant Boophilus microplus (Acari: Ixodidae) cell lines","volume":"39","author":"Cossio-Bayugar","year":"2002","journal-title":"J. Med. Entomol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0020","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1475-2867-5-30","article-title":"ABC transporters as multidrug resistance mechanisms and the development of chemosensitizers for their reversal","volume":"5","author":"Choi","year":"2005","journal-title":"Cancer Cell Int."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0025","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1007\/s004380100531","article-title":"DDT resistance in Drosophila correlates with Cyp6g1 over-expression and confers cross-resistance to the neonicotinoid imidacloprid","volume":"266","author":"Daborn","year":"2001","journal-title":"Mol. Genet. Genomics"},{"key":"10.1016\/j.vetpar.2014.05.042_bib0030","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1093\/jee\/66.1.130","article-title":"Boophilus annulatus and B. microplus: laboratory tests of insecticides","volume":"66","author":"Drummond","year":"1973","journal-title":"J. Econ. Entomol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0035","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1038\/nature06548","article-title":"Resistance to therapy caused by intragenic deletion in BRCA2","volume":"451","author":"Edwards","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.vetpar.2014.05.042_bib0040","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.ibmb.2008.01.006","article-title":"Cellular and molecular characterization of an embryonic cell line (BME26) from the tick Rhipicephalus (Boophilus) microplus","volume":"38","author":"Esteves","year":"2008","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0045","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1097\/QCO.0b013e328010774c","article-title":"Ivermectin: uses and impact 20 years on","volume":"19","author":"Fox","year":"2006","journal-title":"Curr. Opin. Infect. Dis."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0050","first-page":"237","article-title":"Chemotherapy-induced resistance by ATP-binding cassette transporter genes","volume":"1775","author":"Gillet","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.vetpar.2014.05.042_bib0055","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/978-1-60761-416-6_4","article-title":"Mechanisms of multidrug resistance in cancer","volume":"596","author":"Gillet","year":"2010","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0060","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1603\/0022-2585-38.1.44","article-title":"Use of an allele-specific polymerase chain reaction assay to genotype pyrethroid resistant strains of Boophilus microplus (Acari: Ixodidae)","volume":"38","author":"Guerrero","year":"2001","journal-title":"J. Med. Entomol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0065","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/S1984-29612012000100002","article-title":"Acaricide resistance mechanisms in Rhipicephalus (Boophilus) microplus","volume":"21","author":"Guerrero","year":"2012","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0070","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1038\/nature05630","article-title":"Multiple molecular mechanisms for multidrug resistance transporters","volume":"446","author":"Higgins","year":"2007","journal-title":"Nature"},{"key":"10.1016\/j.vetpar.2014.05.042_bib0075","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1006\/jmbi.1999.2993","article-title":"ABC-ATPases, adaptable energy generators fuelling transmembrane movement of a variety of molecules in organisms from bacteria to humans","volume":"293","author":"Holland","year":"1999","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0080","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.ijpara.2008.06.009","article-title":"Increased expression of ABC transport proteins is associated with ivermectin resistance in the model nematode Caenorhabditis elegans","volume":"39","author":"James","year":"2009","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0085","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2006.01.010","article-title":"The productivity effects of cattle tick (Boophilus microplus) infestation on cattle, with particular reference to Bos indicus cattle and their crosses","volume":"137","author":"Jonsson","year":"2006","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0090","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2008.03.022","article-title":"Productivity and health effects of anaplasmosis and babesiosis on Bos indicus cattle and their crosses, and the effects of differing intensity of tick control in Australia","volume":"155","author":"Jonsson","year":"2008","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0095","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1002\/ps.1472","article-title":"Metabolism of imidacloprid and DDT by P450 CYP6G1 expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides in Cyp6g1-overexpressing strains of Drosophila melanogaster, leading to resistance","volume":"64","author":"Joussen","year":"2008","journal-title":"Pest Manag. Sci."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0100","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.vetpar.2006.07.001","article-title":"Larval immersion tests with ivermectin in populations of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) from State of S\u00e3o Paulo, Brazil","volume":"142","author":"Klafke","year":"2006","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0105","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.cbi.2013.03.009","article-title":"Avermectin induces P-glycoprotein expression in S2 cells via the calcium\/calmodulin\/NF-\u03baB pathway","volume":"203","author":"Luo","year":"2013","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0110","first-page":"64","article-title":"Avermectin resistance of the cattle tick Boophilus microplus in Brazil","volume":"149","author":"Martins","year":"2001","journal-title":"Vet. Rec."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0115","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.bcp.2011.10.010","article-title":"Ivermectin induces P-glycoprotein expression and function through mRNA stabilization in murine hepatocyte cell line","volume":"83","author":"M\u00e9nez","year":"2012","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0120","first-page":"3","article-title":"Increased transcription of glutathione S-transferases in acaricide exposed scabies mites","volume":"18","author":"Mounsey","year":"2010","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.vetpar.2014.05.042_bib0125","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.vetpar.2009.10.021","article-title":"First report of the cattle tick Rhipicephalus microplus resistant to ivermectin in Mexico","volume":"168","author":"Perez-Cogollo","year":"2010","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0130","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0135","doi-asserted-by":"crossref","first-page":"01","DOI":"10.1093\/nar\/30.9.e36","article-title":"Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR","volume":"30","author":"Pfaffl","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0140","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.cbpb.2008.08.008","article-title":"An extraovarian aspartic protease accumulated in tick oocytes with vitellin-degradation activity","volume":"151","author":"Pohl","year":"2008","journal-title":"Comp. Biochem. Physiol. B: Biochem. Mol. Biol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0145","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/j.ijpara.2011.08.004","article-title":"ABC transporter efflux pumps: a defense mechanism against ivermectin in Rhipicephalus (Boophilus) microplus","volume":"41","author":"Pohl","year":"2011","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0150","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1007\/s00436-012-3089-1","article-title":"ABC transporters as a multidrug detoxification mechanism in Rhipicephalus (Boophilus) microplus","volume":"111","author":"Pohl","year":"2012","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0155","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1017\/S0007485300027358","article-title":"Detection of esterase activity in susceptible and organophosphate resistant strains of the cattle tick Boophilus microplus","volume":"87","author":"Rosario-Cruz","year":"1997","journal-title":"Bull. Entomol. Res."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0160","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1038\/nrd1984","article-title":"Targeting multidrug resistance in cancer","volume":"5","author":"Szak\u00e1cs","year":"2006","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0165","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.drudis.2007.12.010","article-title":"The role of ABC transporters in drug absorption, distribution, metabolism, excretion and toxicity (ADME-Tox)","volume":"13","author":"Szak\u00e1cs","year":"2008","journal-title":"Drug Discov. Today"},{"key":"10.1016\/j.vetpar.2014.05.042_bib0170","doi-asserted-by":"crossref","first-page":"967419","DOI":"10.4061\/2011\/967419","article-title":"Multidrug resistance in breast cancer: from in vitro models to clinical studies","volume":"2011","author":"Wind","year":"2011","journal-title":"Int. J. Breast Cancer"},{"key":"10.1016\/j.vetpar.2014.05.042_bib0175","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0166-6851(97)00215-6","article-title":"Ivermectin resistance in nematodes may be caused by alteration of P-glycoprotein homolog","volume":"91","author":"Xu","year":"1998","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2014.05.042_bib0180","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1111\/j.1365-2583.2011.01097.x","article-title":"Brief exposures of human body lice to sublethal amounts of ivermectin over-transcribes detoxification genes involved in tolerance","volume":"20","author":"Yoon","year":"2011","journal-title":"Insect Mol. Biol."}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401714003392?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401714003392?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T00:29:27Z","timestamp":1589243367000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401714003392"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8]]},"references-count":36,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["S0304401714003392"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2014.05.042","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2014,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In vitro establishment of ivermectin-resistant Rhipicephalus microplus cell line and the contribution of ABC transporters on the resistance mechanism","name":"articletitle","label":"Article Title"},{"value":"Veterinary Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetpar.2014.05.042","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier B.V.","name":"copyright","label":"Copyright"}]}}