{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T12:52:59Z","timestamp":1774270379000,"version":"3.50.1"},"reference-count":33,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"S\u00e3o Paulo Research Foundation (Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo \u2013 FAPESP)","award":["2009\/51595-9"],"award-info":[{"award-number":["2009\/51595-9"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2014,9]]},"DOI":"10.1016\/j.vetpar.2014.07.033","type":"journal-article","created":{"date-parts":[[2014,8,10]],"date-time":"2014-08-10T10:01:55Z","timestamp":1407664915000},"page":"7-13","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":29,"title":["Diagnosis of gastric cryptosporidiosis in birds using a duplex real-time PCR assay"],"prefix":"10.1016","volume":"205","author":[{"given":"Alex A.","family":"Nakamura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila G.","family":"Homem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana M.J.","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0063-5172","authenticated-orcid":false,"given":"Marcelo V.","family":"Meireles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2014.07.033_bib0005","doi-asserted-by":"crossref","first-page":"14916","DOI":"10.1007\/s00436-010-1810-5","article-title":"Multilocus genotypic analysis of Cryptosporidium isolates from cockatiels in Japan","volume":"106","author":"Abe","year":"2010","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0010","doi-asserted-by":"crossref","first-page":"7026","DOI":"10.1637\/8356-051208-Case.1","article-title":"Natural infection with Cryptosporidium galli in canaries (Serinus canaria), in a cockatiel (Nymphicus hollandicus) and in lesser seed-finches (Oryzoborus angolensis) from Brazil","volume":"52","author":"Antunes","year":"2008","journal-title":"Avian Dis."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0015","unstructured":"Applied Biosystems, n.d. Creating standard curves with genomic DNA or plasmid DNA templates for use in quantitative PCR. [Online]. Available: http:\/\/www6.appliedbiosystems.com\/support\/tutorials\/pdf\/quant_pcr.pdf (accessed 17.07.14)."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0020","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.2307\/1591401","article-title":"Cryptosporidium sp. infection in the proventriculus of an Australian diamond firetail finch (Staganoplura bella Passeriformes, Estrildidae)","volume":"34","author":"Blagburn","year":"1990","journal-title":"Avian Dis."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0025","first-page":"12116","article-title":"Novel quantitative TaqMan real-time PCR assays for detection of Cryptosporidium at the genus level and genotyping of major human and cattle-infecting species","volume":"114","author":"Burnet","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0030","series-title":"Cryptosporidiosis of Man and Animals","first-page":"59","article-title":"Techniques and laboratory maintenance of Cryptosporidium","author":"Current","year":"1990"},{"key":"10.1016\/j.vetpar.2014.07.033_bib0035","doi-asserted-by":"crossref","first-page":"2896","DOI":"10.1111\/j.1550-7408.1986.tb05608.x","article-title":"The life cycle of Cryptosporidium baileyi n. sp. (Apicomplexa, Cryptosporidiidae) in infected chickens","volume":"33","author":"Current","year":"1986","journal-title":"J. Protozool."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0040","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1128\/JCM.01424-10","article-title":"Age-stratified Bayesian analysis estimating the sensitivity and specificity of four diagnostic tests for the detection of Cryptosporidium oocysts in neonatal calves","volume":"49","author":"De Waele","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0045","doi-asserted-by":"crossref","first-page":"9186","DOI":"10.1128\/JCM.01733-10","article-title":"Detection and differentiation of Cryptosporidium spp. in human clinical samples using real-time PCR","volume":"49","author":"Hadfield","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0050","first-page":"956","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0055","doi-asserted-by":"crossref","first-page":"17416","DOI":"10.1007\/s00436-011-2694-8","article-title":"Real-time PCR assay targeting the actin gene for the detection of Cryptosporidium parvum in calf fecal samples","volume":"110","author":"Homem","year":"2012","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0060","doi-asserted-by":"crossref","first-page":"4526","DOI":"10.1128\/AEM.70.1.452-458.2004","article-title":"Phylogenetic analyses of the hypervariable region of the 18s rRNA gene of Cryptosporidium oocysts in the feces of Canada Geese (Branta canadensis): evidence for five novel genotypes","volume":"70","author":"Jellison","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0065","doi-asserted-by":"crossref","first-page":"10996","DOI":"10.1099\/jmm.0.2008\/001461-0","article-title":"Detection and differentiation of Cryptosporidium hominis and Cryptosporidium parvum by dual TaqMan assays","volume":"57","author":"Jothikumar","year":"2008","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0070","doi-asserted-by":"crossref","first-page":"11026","DOI":"10.1637\/9227-123009-Case.1","article-title":"Cryptosporidium avian genotype III as a possible causative agent of chronic vomiting in peach-faced lovebirds (Agapornis roseicollis)","volume":"54","author":"Makino","year":"2010","journal-title":"Avian Dis."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0075","doi-asserted-by":"crossref","first-page":"6236","DOI":"10.1645\/0022-3395(2006)92[623:BSAMCO]2.0.CO;2","article-title":"Biological studies and molecular characterization of a Cryptosporidium isolate from ostrich (Struthio camelus)","volume":"92","author":"Meireles","year":"2006","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0080","doi-asserted-by":"crossref","first-page":"2896","DOI":"10.1016\/S0020-7519(00)00164-8","article-title":"Molecular and phylogenetic characterization of Cryptosporidium from birds","volume":"31","author":"Morgan","year":"2001","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0085","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.vetpar.2009.07.033","article-title":"Molecular characterization of Cryptosporidium spp. from fecal samples of birds kept in captivity in Brazil","volume":"166","author":"Nakamura","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0090","doi-asserted-by":"crossref","first-page":"75486","DOI":"10.1128\/AEM.01352-06","article-title":"Identification of novel Cryptosporidium genotypes from avian hosts","volume":"72","author":"Ng","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0095","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.exppara.2012.10.010","article-title":"Prevalence and molecular characterization of Cryptosporidium in ostriches (Struthio camelus) on a farm in central Vietnam","volume":"133","author":"Nguyen","year":"2013","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0100","first-page":"103","article-title":"Findings of Cryptosporidia in the stomach of chickens and of exotic and wild birds","volume":"51","author":"Pavl\u00e1sek","year":"2001","journal-title":"Veterinarstvi"},{"key":"10.1016\/j.vetpar.2014.07.033_bib0105","doi-asserted-by":"crossref","first-page":"3366","DOI":"10.1016\/j.exppara.2011.04.003","article-title":"Cryptosporidium spp. in pet birds: genetic diversity and potential public health significance","volume":"128","author":"Qi","year":"2011","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0110","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.exppara.2009.02.002","article-title":"Cryptosporidium in birds, fish and amphibians","volume":"124","author":"Ryan","year":"2010","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0115","doi-asserted-by":"crossref","first-page":"8096","DOI":"10.1645\/GE-74RI","article-title":"A redescription of Cryptosporidium galli Pavlasek, 1999 (Apicomplexa: Cryptosporidiidae) in birds","volume":"89","author":"Ryan","year":"2003","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0120","first-page":"1136","article-title":"Cryptosporidium infection in ostriches (Struthio camelus) in Brazil: clinical, morphological and molecular studies","volume":"7","author":"Santos","year":"2005","journal-title":"Braz. J. Poultry Sci."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0125","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.vetpar.2010.09.031","article-title":"Genotyping of Cryptosporidium spp. from free-living wild birds in Brazil","volume":"175","author":"Sev\u00e1","year":"2011","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0130","doi-asserted-by":"crossref","first-page":"2716","DOI":"10.1007\/s00436-010-1858-2","article-title":"Physical, epidemiological, and molecular evaluation of infection by Cryptosporidium galli in Passeriformes","volume":"107","author":"Silva","year":"2010","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0135","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/S0368-1742(55)80025-2","article-title":"Cryptosporidium meleagridis (sp. nov.)","volume":"65","author":"Slavin","year":"1955","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0140","first-page":"48766","article-title":"The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality tools","volume":"24","author":"Thompson","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0145","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1128\/AEM.65.4.1578-1583.1999","article-title":"Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus","volume":"65","author":"Xiao","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0150","doi-asserted-by":"crossref","first-page":"54926","DOI":"10.1128\/AEM.66.12.5492-5498.2000","article-title":"Identification of species and sources of Cryptosporidium oocysts in storm waters using a small-subunit rRNA-based diagnostic and genotyping tool","volume":"66","author":"Xiao","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0155","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1128\/CMR.17.1.72-97.2004","article-title":"Cryptosporidium taxonomy: recent advances and implications for public health","volume":"17","author":"Xiao","year":"2004","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0160","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.vetpar.2008.12.021","article-title":"Prevalence and molecular characterization of Cryptosporidium and Giardia species in pre-weaned sheep in Australia","volume":"161","author":"Yang","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2014.07.033_bib0165","doi-asserted-by":"crossref","first-page":"4211","DOI":"10.1128\/AEM.70.7.4211-4215.2004","article-title":"Host-Adapted Cryptosporidium spp. in Canada Geese (Branta canadensis)","volume":"70","author":"Zhou","year":"2004","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401714004208?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401714004208?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,8,23]],"date-time":"2020-08-23T02:29:06Z","timestamp":1598149746000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401714004208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9]]},"references-count":33,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["S0304401714004208"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2014.07.033","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2014,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Diagnosis of gastric cryptosporidiosis in birds using a duplex real-time PCR assay","name":"articletitle","label":"Article Title"},{"value":"Veterinary Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetpar.2014.07.033","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}