{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:40:18Z","timestamp":1792784418780,"version":"4.5.0"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"National Key Basic Research Program","award":["2015CB150300"],"award-info":[{"award-number":["2015CB150300"]}]},{"DOI":"10.13039\/501100010581","name":"General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China","doi-asserted-by":"crossref","award":["2009IK007"],"award-info":[{"award-number":["2009IK007"]}],"id":[{"id":"10.13039\/501100010581","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2016,3]]},"DOI":"10.1016\/j.vetpar.2016.02.004","type":"journal-article","created":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T16:15:48Z","timestamp":1454516148000},"page":"71-76","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"special_numbering":"C","title":["Rapid and sensitive detection of Babesia bovis and Babesia bigemina by loop-mediated isothermal amplification combined with a lateral flow dipstick"],"prefix":"10.1016","volume":"219","author":[{"given":"Yimin","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqiu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3796-6621","authenticated-orcid":false,"given":"Aifang","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2016.02.004_bib0005","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.vetpar.2006.08.014","article-title":"Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis","volume":"143","author":"Alhassan","year":"2007","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0010","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S0304-4017(01)00464-2","article-title":"Bovine piroplasms in Minorca (Balearic Islands, Spain): a comparison of PCR-based and light microscopy detection","volume":"99","author":"Almeria","year":"2001","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0015","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.vetpar.2008.09.012","article-title":"Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing","volume":"158","author":"Bakheit","year":"2008","journal-title":"Vet. Parasitol."},{"issue":"Suppl","key":"10.1016\/j.vetpar.2016.02.004_bib0020","doi-asserted-by":"crossref","first-page":"S247","DOI":"10.1017\/S0031182004005190","article-title":"Babesiosis of cattle","volume":"129","author":"Bock","year":"2004","journal-title":"Parasitology"},{"key":"10.1016\/j.vetpar.2016.02.004_bib0025","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0304-4017(94)03111-9","article-title":"Current state and future-trends in the diagnosis of Babesiosis","volume":"57","author":"Bose","year":"1995","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0030","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/0020-7519(94)90081-7","article-title":"Diagnosis of Babesia caballi infections in horses by enzyme-linked immunosorbent assay (ELISA) and western blot","volume":"24","author":"Bose","year":"1994","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0035","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.vetpar.2007.03.031","article-title":"A quantitative PCR assay for the detection and quantification of Babesia bovis and B bigemina","volume":"147","author":"Buling","year":"2007","journal-title":"Vet. parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0040","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1093\/genetics\/136.3.857","article-title":"The small ribosomal subunit RNA isoforms in Plasmodium cynomolgi","volume":"136","author":"Corredor","year":"1994","journal-title":"Genetics"},{"key":"10.1016\/j.vetpar.2016.02.004_bib0045","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/0166-6851(90)90136-A","article-title":"Cloning and characterization of the rRNA genes and flanking regions from Babesia bovis: use of the genes as strain discriminating probes","volume":"43","author":"Dalrymple","year":"1990","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0050","doi-asserted-by":"crossref","first-page":"6984","DOI":"10.1016\/S0021-9258(18)32322-6","article-title":"The four ribosomal DNA units of the malaria parasite Plasmodium berghei. Identification, restriction map, and copy number analysis","volume":"258","author":"Dame","year":"1983","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0055","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.jviromet.2014.12.006","article-title":"Rapid and simple detection of Japanese encephalitis virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick","volume":"213","author":"Deng","year":"2015","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.vetpar.2016.02.004_bib0060","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1007\/s00705-010-0593-4","article-title":"Rapid and sensitive detection of infectious spleen and kidney necrosis virus by loop-mediated isothermal amplification combined with a lateral flow dipstick","volume":"155","author":"Ding","year":"2010","journal-title":"Arch. Virol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0065","doi-asserted-by":"crossref","first-page":"4359","DOI":"10.1128\/JCM.41.9.4359-4365.2003","article-title":"Use of loop-mediated isothermal amplification of the IS900 sequence for rapid detection of cultured Mycobacterium avium subsp. paratuberculosis","volume":"41","author":"Enosawa","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0070","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1128\/JCM.30.6.1374-1379.1992","article-title":"Detection of Babesia bovis carrier cattle by using polymerase chain reaction amplification of parasite DNA","volume":"30","author":"Fahrimal","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0075","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.1998.tb11027.x","article-title":"Bovine babesiosis and anaplasmosis follow-up on cattle relocated in an endemic area for hemoparasitic diseases","volume":"849","author":"Figueroa","year":"1998","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0080","doi-asserted-by":"crossref","first-page":"2576","DOI":"10.1128\/JCM.30.10.2576-2582.1992","article-title":"Detection of Babesia bigemina-infected carriers by polymerase chain reaction amplification","volume":"30","author":"Figueroa","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0085","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.actatropica.2012.10.007","article-title":"Detection of Toxoplasma gondii oocysts in different water resources by loop mediated isothermal amplification (LAMP)","volume":"125","author":"Gallas-Lindemann","year":"2013","journal-title":"Acta Trop."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0090","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.vetpar.2009.06.036","article-title":"Loop-mediated isothermal amplification (LAMP) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) in China","volume":"165","author":"He","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0095","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1128\/CMR.13.3.451-469.2000","article-title":"Babesiosis","volume":"13","author":"Homer","year":"2000","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0100","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/j.ijpara.2008.03.001","article-title":"Babesiosis: recent insights into an ancient disease","volume":"38","author":"Hunfeld","year":"2008","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0105","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1128\/JCM.42.6.2465-2469.2004","article-title":"Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method","volume":"42","author":"Ikadai","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0110","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.mcp.2008.12.003","article-title":"Rapid and sensitive detection of white spot syndrome virus by loop-mediated isothermal amplification combined with a lateral flow dipstick","volume":"23","author":"Jaroenram","year":"2009","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.vetpar.2016.02.004_bib0115","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.pt.2009.07.010","article-title":"LAMP\u2014a powerful and flexible tool for monitoring microbial pathogens","volume":"25","author":"Karanis","year":"2009","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0120","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jviromet.2012.11.041","article-title":"Rapid and sensitive detection of shrimp yellow head virus by loop-mediated isothermal amplification combined with a lateral flow dipstick","volume":"188","author":"Khunthong","year":"2013","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.vetpar.2016.02.004_bib0125","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.jviromet.2008.06.025","article-title":"Shrimp Taura syndrome virus detection by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick","volume":"153","author":"Kiatpathomchai","year":"2008","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.vetpar.2016.02.004_bib0130","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/0166-6851(94)90152-X","article-title":"Evidence for two single copy units in Theileria parva ribosomal RNA genes","volume":"66","author":"Kibe","year":"1994","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0135","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0166-6851(97)00181-3","article-title":"Ribosomal RNA gene organization in Cryptosporidium parvum","volume":"90","author":"Le Blancq","year":"1997","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0140","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/j.parint.2012.07.004","article-title":"Loop-mediated isothermal amplification (LAMP) method based on two species-specific primer sets for the rapid identification of Chinese Babesia bovis and B. bigemina","volume":"61","author":"Liu","year":"2012","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0145","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.vetpar.2015.02.008","article-title":"Development of loop-mediated isothermal amplification (LAMP) for detection of Babesia gibsoni infection in dogs","volume":"209","author":"Mandal","year":"2015","journal-title":"Vet. Parasitol."},{"issue":"Suppl. 3","key":"10.1016\/j.vetpar.2016.02.004_bib0150","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1590\/S0074-02761992000700033","article-title":"New methods for the diagnosis of Babesia bigemina infection","volume":"87","author":"Morzaria","year":"1992","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.vetpar.2016.02.004_bib0155","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1865-1682.2010.01113.x","article-title":"Development of a loop-mediated isothermal amplification (LAMP) assay for rapid diagnosis of Babesia canis infections","volume":"57","author":"Muller","year":"2010","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0160","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.diagmicrobio.2010.08.026","article-title":"Rapid and sensitive detection of human African trypanosomiasis by loop-mediated isothermal amplification combined with a lateral-flow dipstick","volume":"69","author":"Njiru","year":"2011","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0165","doi-asserted-by":"crossref","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0170","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.vetpar.2006.01.036","article-title":"Babesiosis of wild carnivores and ungulates","volume":"138","author":"Penzhorn","year":"2006","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0175","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/1756-3305-3-36","article-title":"One Health approach to identify research needs in bovine and human babesioses: workshop report","volume":"3","author":"Perez de Leon","year":"2010","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.vetpar.2016.02.004_bib0180","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1007\/s00436-009-1391-3","article-title":"Rapid identification of Giardia duodenalis by loop-mediated isothermal amplification (LAMP) from faecal and environmental samples and comparative findings by PCR and real-time PCR methods","volume":"104","author":"Plutzer","year":"2009","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0185","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.jviromet.2008.10.018","article-title":"Detection of shrimp infectious myonecrosis virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick","volume":"156","author":"Puthawibool","year":"2009","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.vetpar.2016.02.004_bib0190","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/1471-2180-14-86","article-title":"Rapid and sensitive detection of Candidatus Liberibacter asiaticus by loop mediated isothermal amplification combined with a lateral flow dipstick","volume":"14","author":"Rigano","year":"2014","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.vetpar.2016.02.004_bib0195","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1006\/mcpr.1998.0223","article-title":"Development and evaluation of an extra chromosomal DNA-based PCR test for diagnosing bovine babesiosis","volume":"13","author":"Salem","year":"1999","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.vetpar.2016.02.004_bib0200","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1186\/s13071-015-1202-x","article-title":"Development of loop-mediated isothermal amplification (LAMP) for simple detection of Leishmania infection","volume":"8","author":"Sriworarat","year":"2015","journal-title":"Parasites Vectors"},{"issue":"Suppl. 3","key":"10.1016\/j.vetpar.2016.02.004_bib0205","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1590\/S0074-02761992000700032","article-title":"Non-immunologic methods of diagnosis of babesiosis","volume":"87","author":"Wagner","year":"1992","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.vetpar.2016.02.004_bib0210","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/j.parint.2014.06.004","article-title":"Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax","volume":"63","author":"Yongkiettrakul","year":"2014","journal-title":"Parasitol. Int."}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401716300280?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401716300280?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T01:18:47Z","timestamp":1654219127000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401716300280"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3]]},"references-count":42,"alternative-id":["S0304401716300280"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2016.02.004","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2016,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid and sensitive detection of Babesia bovis and Babesia bigemina by loop-mediated isothermal amplification combined with a lateral flow dipstick","name":"articletitle","label":"Article Title"},{"value":"Veterinary Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetpar.2016.02.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}