{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T03:06:53Z","timestamp":1781060813701,"version":"3.54.1"},"reference-count":21,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T00:00:00Z","timestamp":1468540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011102","name":"EU Seventh Framework Programme","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2016,8]]},"DOI":"10.1016\/j.vetpar.2016.07.018","type":"journal-article","created":{"date-parts":[[2016,7,17]],"date-time":"2016-07-17T07:05:39Z","timestamp":1468739139000},"page":"26-29","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":27,"special_numbering":"C","title":["Development of a sensitive method to extract and detect low numbers of Cryptosporidium oocysts from adult cattle faecal samples"],"prefix":"10.1016","volume":"227","author":[{"given":"B.","family":"Wells","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Thomson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Ensor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.A.","family":"Innes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Katzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2016.07.018_bib0005","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1645\/GE-3192RN","article-title":"Lack of detectable shedding of Cryptosporidium parvum oocysts by periparturient dairy cattle","volume":"89","author":"Atwill","year":"2003","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0010","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1099\/jmm.0.034181-0","article-title":"Comparison of diagnostic sensitivity and specificity of seven Cryptosporidium assays used in the UK","volume":"60","author":"Chalmers","year":"2011","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0015","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.pt.2013.03.001","article-title":"Looking for Cryptosporidium: the application of advances in detection and diagnosis","volume":"29","author":"Chalmers","year":"2013","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0020","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.vetpar.2012.05.002","article-title":"Peri-parturient rise of Cryptosporidium oocysts in cows: new insights provided by duplex quantitative real-time PCR","volume":"189","author":"DE Waele","year":"2012","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0025","doi-asserted-by":"crossref","first-page":"5581","DOI":"10.1128\/AEM.67.12.5581-5584.2001","article-title":"Modification of a rapid method for the identification of gene-specific polymorphisms in Cryptosporidium parvum and its application to clinical and epidemiological investigations","volume":"67","author":"Elwin","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0030","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1645\/0022-3395(2000)086[0495:NTOCPB]2.0.CO;2","article-title":"Natural transmission of Cryptosporidium parvum between dams and calves on a dairy farm","volume":"86","author":"Faubert","year":"2000","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0035","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.vetpar.2006.12.009","article-title":"Prevalence of Cryptosporidium species and genotypes in mature dairy cattle on farms in eastern United States compared with younger cattle from the same locations","volume":"145","author":"Fayer","year":"2007","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0040","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0304-4017(00)00356-3","article-title":"Prevalence of Cryptosporidium: giardia and Eimeria infections in post-weaned and adult cattle on three Maryland farms","volume":"93","author":"Fayer","year":"2000","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0045","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/biomet\/80.1.27","article-title":"Bias reduction of maximum likelihood estimates","volume":"80","author":"Firth","year":"1993","journal-title":"Biometrika"},{"key":"10.1016\/j.vetpar.2016.07.018_bib0050","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0304-4017(93)90171-I","article-title":"Detection of oocysts and IgG antibodies to Cryptosporidium parvum in asymptomatic adult cattle","volume":"47","author":"Lorenzo Lorenzo","year":"1993","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0055","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0166-6851(99)00101-2","article-title":"A high level of mixed Trypanosoma brucei infections in tsetse flies detected by three hypervariable microsatellites","volume":"102","author":"MacLeod","year":"1999","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0060","doi-asserted-by":"crossref","first-page":"5003","DOI":"10.1128\/IAI.62.11.5003-5009.1994","article-title":"Age-related resistance in ovine cryptosporidiosis: patterns of infection and humoral immune response","volume":"62","author":"Ortega-Mora","year":"1994","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0065","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1111\/j.1751-0813.2010.00631.x","article-title":"Cryptosporidium andersoni in Western Australian feedlot cattle","volume":"88","author":"Ralston","year":"2010","journal-title":"Aust. Vet. J."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0070","doi-asserted-by":"crossref","first-page":"3423","DOI":"10.1007\/s00436-013-3520-2","article-title":"Molecular characterization of Cryptosporidium isolates from high-excreting young dairy calves in dairy cattle herds in Western France","volume":"112","author":"Rieux","year":"2013","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0075","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1017\/S0031182000046990","article-title":"Methods in coccidiosis research: separation of oocysts from faeces","volume":"73","author":"Ryley","year":"1976","journal-title":"Parasitology"},{"key":"10.1016\/j.vetpar.2016.07.018_bib0080","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/00480169.2012.731681","article-title":"Clinical and subclinical infections with Cryptosporidium in animals","volume":"61","author":"Santin","year":"2013","journal-title":"N. Z. Vet. J."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0085","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1017\/S0950268812000830","article-title":"Cryptosporidium spp. in calves and cows from organic and conventional dairy herds","volume":"141","author":"Silverlas","year":"2013","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0090","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.vetpar.2014.05.022","article-title":"Prevalence and molecular typing of: cryptosporidium in dairy cattle in England and Wales and examination of potential on-farm transmission routes","volume":"204","author":"Smith","year":"2014","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0095","article-title":"A multiplex PCR test to identify four common cattle adapted Cryptosporidium species","author":"Thomson","year":"2016","journal-title":"Parasitology"},{"key":"10.1016\/j.vetpar.2016.07.018_bib0100","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.parint.2010.09.002","article-title":"Cryptosporidium andersoni is the predominant species in post-weaned and adult dairy cattle in China","volume":"60","author":"Wang","year":"2011","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.vetpar.2016.07.018_bib0105","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s13071-015-0684-x","article-title":"Prevalence, species identification and genotyping Cryptosporidium from livestock and deer in a catchment in the Cairngorms with a history of a contaminated public water supply","volume":"8","author":"Wells","year":"2015","journal-title":"Parasites Vectors"}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401716302771?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401716302771?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T22:17:39Z","timestamp":1600899459000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401716302771"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8]]},"references-count":21,"alternative-id":["S0304401716302771"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2016.07.018","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2016,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a sensitive method to extract and detect low numbers of Cryptosporidium oocysts from adult cattle faecal samples","name":"articletitle","label":"Article Title"},{"value":"Veterinary Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetpar.2016.07.018","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}