{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:12:36Z","timestamp":1792789956991,"version":"4.5.0"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100009570","name":"Srinakharinwirot University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009570","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2019,10]]},"DOI":"10.1016\/j.vetpar.2019.108922","type":"journal-article","created":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T09:25:14Z","timestamp":1567934714000},"page":"108922","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Development of Cytochrome B, a new candidate gene for a high accuracy detection of Fasciola eggs in fecal specimens"],"prefix":"10.1016","volume":"274","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4241-6966","authenticated-orcid":false,"given":"Thapana","family":"Chontananarth","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janjura","family":"Parawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2019.108922_bib0005","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1179\/136485910X12607012373713","article-title":"Specific PCR-based assays for the identification of Fasciola species: their development, evaluation and potential usefulness in prevalence surveys","volume":"104","author":"Ai","year":"2010","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"10.1016\/j.vetpar.2019.108922_bib0010","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1007\/s12161-013-9672-y","article-title":"Canine-specific PCR assay targeting cytochrome b gene for the detection of dog meat adulteration in commercial frankfurters","volume":"7","author":"Ali","year":"2014","journal-title":"Food Anal. Methods"},{"key":"10.1016\/j.vetpar.2019.108922_bib0015","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.foodchem.2014.12.098","article-title":"Multiplex PCR assay for the detection of five meat species forbidden in Islamic foods","volume":"177","author":"Ali","year":"2015","journal-title":"Food Chem."},{"key":"10.1016\/j.vetpar.2019.108922_bib0020","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1136\/jcp.23.6.545","article-title":"Further observations on the formol-ether concentration technique for faecal parasites","volume":"23","author":"Allen","year":"1970","journal-title":"J. Clin. Pathol."},{"key":"10.1016\/j.vetpar.2019.108922_bib0025","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/j.tmaid.2014.09.006","article-title":"Fascioliasis: a worldwide parasitic disease of importance in travel medicine","volume":"12","author":"Ashrafi","year":"2014","journal-title":"Travel Med. Infect. Dis."},{"key":"10.1016\/j.vetpar.2019.108922_bib0030","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1186\/s13071-016-1589-z","article-title":"Human fascioliasis endemic areas in Argentina: multigene characterisation of the lymnaeid vectors and climatic-environmental assessment of the transmission pattern","volume":"9","author":"Bargues","year":"2016","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.vetpar.2019.108922_bib0035","doi-asserted-by":"crossref","first-page":"76","DOI":"10.2478\/s11687-010-0011-1","article-title":"Determination of ribosomal internal transcribed spacer 2 (ITS2) interspecific markers in Fasciola hepatica, Fascioloides magna, Dicrocoelium dendriticum and Paramphistomum cervi (Trematoda), parasites of wild and domestic ruminants","volume":"47","author":"Bazsalovicsov\u00e1","year":"2010","journal-title":"Helminthologia"},{"key":"10.1016\/j.vetpar.2019.108922_bib0040","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s11230-016-9631-2","article-title":"Molecular approaches to trematode systematics:\u2018best practice\u2019and implications for future study","volume":"93","author":"Blasco-Costa","year":"2016","journal-title":"Syst. Parasitol."},{"key":"10.1016\/j.vetpar.2019.108922_bib0045","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.actatropica.2014.09.007","article-title":"High prevalence of large trematode eggs in schoolchildren in Cambodia","volume":"141","author":"Bless","year":"2015","journal-title":"Acta Trop."},{"key":"10.1016\/j.vetpar.2019.108922_bib0050","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.parint.2012.03.009","article-title":"Identification of Fasciola flukes in Thailand based on their spermatogenesis and nuclear ribosomal DNA, and their intraspecific relationships based on mitochondrial DNA","volume":"61","author":"Chaichanasak","year":"2012","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.vetpar.2019.108922_bib0055","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1017\/S0022149X15000176","article-title":"Molecular evidence shows that the liver fluke Fasciola gigantica is the predominant Fasciola species in ruminants from Pakistan","volume":"90","author":"Chaudhry","year":"2016","journal-title":"J. Helminthol."},{"key":"10.1016\/j.vetpar.2019.108922_bib0060","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1186\/1756-3305-4-176","article-title":"Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory\/secretory antigens in both serum and stool","volume":"4","author":"Demerdash","year":"2011","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.vetpar.2019.108922_bib0065","first-page":"1","article-title":"Flukes: old parasites but new emergence","volume":"1","author":"Dorchies","year":"2006"},{"key":"10.1016\/j.vetpar.2019.108922_bib0070","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/BF00009846","article-title":"Orthocoelium indonesiense, a new species of amphistome from ruminants in Indonesia","volume":"1","author":"Eduardo","year":"1980","journal-title":"Syst. Parasitol."},{"key":"10.1016\/j.vetpar.2019.108922_bib0075","first-page":"188","article-title":"Surveillance of helminthes and molecular phylogeny of Fasciola gigantica infecting goats in Sadat District, Egypt","volume":"2","author":"Elkhatam","year":"2016","journal-title":"IJSRSET."},{"key":"10.1016\/j.vetpar.2019.108922_bib0080","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0304-4017(00)00413-1","article-title":"An experimental study on triclabendazole resistance of Fasciola hepatica in sheep","volume":"95","author":"Gaasenbeek","year":"2001","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2019.108922_bib0085","doi-asserted-by":"crossref","first-page":"431","DOI":"10.3390\/pathogens4030431","article-title":"Fasciola hepatica: histology of the reproductive organs and differential effects of triclabendazole on drug-sensitive and drug-resistant fluke isolates and on flukes from selected field cases","volume":"4","author":"Hanna","year":"2015","journal-title":"Pathogens"},{"key":"10.1016\/j.vetpar.2019.108922_bib0090","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.vetpar.2014.11.016","article-title":"Fasciola hepatica: a comparative survey of adult fluke resistance to triclabendazole, nitroxynil and closantel on selected upland and lowland sheep farms in Northern Ireland using faecal egg counting, coproantigen ELISA testing and fluke histology","volume":"207","author":"Hanna","year":"2015","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2019.108922_bib0095","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1292\/jvms.16-0126","article-title":"Molecular characterization of Fasciola gigantica in Delhi, India and its phylogenetic relation to the species from South Asian countries","volume":"78","author":"Hayashi","year":"2016","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.vetpar.2019.108922_bib0100","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.parint.2015.11.010","article-title":"Molecular characterization and phylogenetic analysis of Fasciola hepatica from Peru","volume":"65","author":"Ichikawa-Seki","year":"2016","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.vetpar.2019.108922_bib0105","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1017\/S003118201600161X","article-title":"Nuclear and mitochondrial DNA analysis reveals that hybridization between Fasciola hepatica and Fasciola gigantica occurred in China","volume":"144","author":"Ichikawa-Seki","year":"2017","journal-title":"Parasitology"},{"key":"10.1016\/j.vetpar.2019.108922_bib0110","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/j.parint.2016.10.010","article-title":"Molecular characterization and phylogenetic analysis of Fasciola gigantica from Nigeria","volume":"66","author":"Ichikawa-Seki","year":"2017","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.vetpar.2019.108922_bib0115","doi-asserted-by":"crossref","first-page":"6825","DOI":"10.7314\/APJCP.2015.16.16.6825","article-title":"Review and current status of Opisthorchis viverrini infection at the community level in Thailand","volume":"16","author":"Kaewpitoon","year":"2015","journal-title":"Asian Pac. J. Cancer Prev."},{"issue":"4","key":"10.1016\/j.vetpar.2019.108922_bib0120","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1128\/jcm.35.4.995-998.1997","article-title":"Complex polysaccharides as PCR inhibitors in feces: helicobacter pylori model","volume":"35","author":"Monteiro","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.vetpar.2019.108922_bib0125","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/j.1751-0813.1995.tb03546.x","article-title":"Resistance of Fasciola hepatica to triclabendazole","volume":"72","author":"Overend","year":"1995","journal-title":"Aust. Vet. J."},{"key":"10.1016\/j.vetpar.2019.108922_bib0130","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/s12639-013-0276-7","article-title":"Genetic characterization of Fasciola gigantica from different geographical regions of India by ribosomal DNA markers","volume":"39","author":"Raina","year":"2015","journal-title":"J. Parasit. Dis."},{"key":"10.1016\/j.vetpar.2019.108922_bib0135","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1071\/AN141032","article-title":"Fasciola gigantica infection in large ruminants in northern Laos: smallholder knowledge and practices","volume":"57","author":"Rast","year":"2017","journal-title":"Anim. Prod. Sci."},{"key":"10.1016\/j.vetpar.2019.108922_bib0140","doi-asserted-by":"crossref","first-page":"64","DOI":"10.2478\/s11687-008-0012-5","article-title":"Review and update of paramphistomosis","volume":"45","author":"Sanabria","year":"2008","journal-title":"Helminthologia"},{"key":"10.1016\/j.vetpar.2019.108922_bib0145","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.parint.2015.12.002","article-title":"Novel methods for the molecular discrimination of Fasciola spp. On the basis of nuclear protein-coding genes","volume":"65","author":"Shoriki","year":"2016","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.vetpar.2019.108922_bib0150","doi-asserted-by":"crossref","first-page":"4615","DOI":"10.1007\/s00216-016-9595-8","article-title":"Advances in DNA metabarcoding for food and wildlife forensic species identification","volume":"408","author":"Staats","year":"2016","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.vetpar.2019.108922_bib0155","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.jip.2016.07.001","article-title":"Species-specific diagnostics of Apis mellifera trypanosomatids: a nine-year survey (2007\u20132015) for trypanosomatids and microsporidians in Serbian honey bees","volume":"139","author":"Stevanovic","year":"2016","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.vetpar.2019.108922_bib0160","first-page":"32","article-title":"Prevalence of amphistome parasites (Trematoda: Digenea) in Udaipur of southern Rajasthan, India","volume":"3","author":"Swarnakar","year":"2014","journal-title":"Int. J. Curr. Microbiol. Appl. Sci."},{"key":"10.1016\/j.vetpar.2019.108922_bib0165","first-page":"1","article-title":"Fasciola gigantica cases in ruminants from Samsun Province, Turkey","volume":"13","author":"Umur","year":"2018","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"10.1016\/j.vetpar.2019.108922_bib0170","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.exppara.2016.02.008","article-title":"Diversity of Sarcocystis spp. shed by opossums in Brazil inferred with phylogenetic analysis of DNA coding ITS1, Cytochrome B, and surface antigens","volume":"164","author":"Valadas","year":"2016","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2019.108922_bib0175","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.exppara.2014.09.006","article-title":"Identification of Fasciola species based on mitochondrial and nuclear DNA reveals the co-existence of intermediate Fasciola and Fasciola gigantica in Thailand","volume":"146","author":"Wannasan","year":"2014","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2019.108922_bib0180","article-title":"SYBR Green real-time PCR-RFLP assay targeting the Plasmodium cytochrome B gene\u2013a highly sensitive molecular tool for malaria parasite detection and species determination","volume":"10","author":"Xu","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.vetpar.2019.108922_bib0185","series-title":"Systema Helminihum. Volume I. The Digenetic Trematodes of Vertebrates. Systema Helminihum. Volume I. The Digenetic Trematodes of Vertebrates","author":"Yamaguti","year":"1958"},{"key":"10.1016\/j.vetpar.2019.108922_bib0190","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1017\/S0022149X16000183","article-title":"The complete mitochondrial genome of Orthocoelium streptocoelium (Digenea: Paramphistomidae) for comparison with other digeneans","volume":"91","author":"Zhao","year":"2017","journal-title":"J. Helminthol."}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401719302031?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401719302031?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T01:32:16Z","timestamp":1759627936000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401719302031"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":38,"alternative-id":["S0304401719302031"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2019.108922","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2019,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of Cytochrome B, a new candidate gene for a high accuracy detection of Fasciola eggs in fecal specimens","name":"articletitle","label":"Article Title"},{"value":"Veterinary Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetpar.2019.108922","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"108922"}}