{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:23:55Z","timestamp":1792002235587,"version":"4.4.0"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100006348","name":"Benem\u00e9rita Universidad Aut\u00f3noma de Puebla","doi-asserted-by":"publisher","award":["VIEP-VIMA-NAT-18-I"],"award-info":[{"award-number":["VIEP-VIMA-NAT-18-I"]}],"id":[{"id":"10.13039\/501100006348","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2021,1]]},"DOI":"10.1016\/j.vetpar.2020.109340","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T09:52:50Z","timestamp":1608285170000},"page":"109340","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"special_numbering":"C","title":["Phage display-based vaccine with cathepsin L and excretory-secretory products mimotopes of Fasciola hepatica induces protective cellular and humoral immune responses in sheep"],"prefix":"10.1016","volume":"289","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5997-5641","authenticated-orcid":false,"given":"Abel","family":"Villa-Mancera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Olivares-P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5499-7449","authenticated-orcid":false,"given":"Agust\u00edn","family":"Olmedo-Ju\u00e1rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Reynoso-Palomar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2020.109340_bib0005","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s12929-016-0285-9","article-title":"Phage display as a promising approach for vaccine development","volume":"23","author":"Aghebati-Maleki","year":"2016","journal-title":"J. Biomed. Sci."},{"key":"10.1016\/j.vetpar.2020.109340_bib0010","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.addr.2018.12.013","article-title":"Phage-based vaccines","volume":"145","author":"Bao","year":"2019","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.vetpar.2020.109340_bib0015","doi-asserted-by":"crossref","first-page":"2775","DOI":"10.1007\/s00436-017-5588-6","article-title":"Excretory\/secretory products from two Fasciola hepatica isolates induce different transcriptional changes and IL-10 release in LPS-activated bovine \"BOMA\" macrophages","volume":"116","author":"Baska","year":"2017","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2020.109340_bib0020","doi-asserted-by":"crossref","first-page":"59","DOI":"10.3389\/fcimb.2016.00059","article-title":"Toxoplasma gondii-derived synthetic peptides containing B- and T-cell epitopes from GRA2 protein are able to enhance mice survival in a model of experimental toxoplasmosis","volume":"6","author":"Bastos","year":"2016","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"Suppl 1","key":"10.1016\/j.vetpar.2020.109340_bib0025","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/tbed.12682","article-title":"Fasciola and fasciolosis in ruminants in Europe: identifying research needs","volume":"65","author":"Beesley","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.vetpar.2020.109340_bib0030","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.ijpara.2010.09.003","article-title":"Relative importance of management, meteorological and environmental factors in the spatial distribution of Fasciola hepatica in dairy cattle in a temperate climate zone","volume":"41","author":"Bennema","year":"2011","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0035","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.parint.2009.12.005","article-title":"Immune response of goats immunised with glutathione S-transferase and experimentally challenged with Fasciola hepatica","volume":"59","author":"Buffoni","year":"2010","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.vetpar.2020.109340_bib0040","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.vetpar.2011.09.027","article-title":"Humoral immune response in goats immunised with cathepsin L1, peroxiredoxin and Sm14 antigen and experimentally challenged with Fasciola hepatica","volume":"185","author":"Buffoni","year":"2012","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0045","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1089\/vim.2017.0049","article-title":"Adjuvants for animal vaccines","volume":"31","author":"Burakova","year":"2018","journal-title":"Viral Immunol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0050","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1590\/s1984-296120180023","article-title":"Sheep polyclonal antibody to map Haemonchus contortus mimotopes using phage display library","volume":"27","author":"Buzatti","year":"2018","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"10.1016\/j.vetpar.2020.109340_bib0055","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005840","article-title":"Cysteine proteases as digestive enzymes in parasitic helminths","volume":"12","author":"Caffrey","year":"2018","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.vetpar.2020.109340_bib0060","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1017\/S0031182013001662","article-title":"Recent advances in the diagnosis, impact on production and prediction of Fasciola hepatica in cattle","volume":"141","author":"Charlier","year":"2014","journal-title":"Parasitology"},{"key":"10.1016\/j.vetpar.2020.109340_bib0065","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0120847","article-title":"Selection of peptide mimics of HIV-1 epitope recognized by neutralizing antibody VRC01","volume":"10","author":"Chikaev","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.vetpar.2020.109340_bib0070","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1111\/pim.12343","article-title":"A prospective view of animal and human Fasciolosis","volume":"38","author":"Cwiklinski","year":"2016","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0075","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/bs.apar.2019.01.001","article-title":"The cathepsin-like cysteine peptidases of trematodes of the genus Fasciola","volume":"104","author":"Cwiklinski","year":"2019","journal-title":"Adv. Parasitol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0080","series-title":"The PyMOL Molecular Graphics System","author":"DeLano","year":"2002"},{"key":"10.1016\/j.vetpar.2020.109340_bib0085","first-page":"65","article-title":"Phage-displayed mimotopes elicit monoclonal antibodies specific for a malaria vaccine candidate","volume":"379","author":"Demangel","year":"1998","journal-title":"Biol. Chem."},{"key":"10.1016\/j.vetpar.2020.109340_bib0090","doi-asserted-by":"crossref","first-page":"32796","DOI":"10.1038\/srep32796","article-title":"Across intra-mammalian stages of the liver f luke Fasciola hepatica: a proteomic study","volume":"6","author":"Di Maggio","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.vetpar.2020.109340_bib0095","series-title":"Pathophysiological Aspects of Proteases","first-page":"657","article-title":"Cysteine proteases of parasitic helminths","author":"Dixit","year":"2017"},{"key":"10.1016\/j.vetpar.2020.109340_bib0100","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/j.1365-3024.2011.01317.x","article-title":"Phage-display library biopanning as a novel approach to identifying nematode vaccine antigens","volume":"34","author":"Ellis","year":"2012","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0105","first-page":"39","article-title":"Drug resistance in liver flukes. International journal for parasitology","volume":"12","author":"Fairweather","year":"2020","journal-title":"Drugs Drug Resist."},{"key":"10.1016\/j.vetpar.2020.109340_bib0110","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1016\/j.vaccine.2018.01.020","article-title":"Antibody recognition of cathepsin L1-derived peptides in Fasciola hepatica-infected and\/or vaccinated cattle and identification of protective linear B-cell epitopes","volume":"36","author":"Garza-Cuartero","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2020.109340_bib0115","doi-asserted-by":"crossref","first-page":"5551","DOI":"10.1016\/j.vaccine.2010.06.039","article-title":"Protection of cattle against a natural infection of Fasciola hepatica by vaccination with recombinant cathepsin L1 (rFhCL1)","volume":"28","author":"Golden","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2020.109340_bib0120","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1007\/s00436-010-1786-1","article-title":"Mapping of Taenia solium TSOL18 antigenic epitopes by phage display library","volume":"106","author":"Guo","year":"2010","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2020.109340_bib0125","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.vaccine.2008.10.035","article-title":"The effect of Quil A adjuvant on the course of experimental Fasciola hepatica infection in sheep","volume":"27","author":"Hacariz","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2020.109340_bib0130","doi-asserted-by":"crossref","first-page":"755","DOI":"10.3389\/fmicb.2015.00755","article-title":"Beyond phage display: non-traditional applications of the filamentous bacteriophage as a vaccine carrier, therapeutic biologic, and bioconjugation scaffold","volume":"6","author":"Henry","year":"2015","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0135","doi-asserted-by":"crossref","first-page":"19","DOI":"10.4062\/biomolther.2012.20.1.019","article-title":"Cyclic peptides as therapeutic agents and biochemical tools","volume":"20","author":"Joo","year":"2012","journal-title":"Biomol. Ther. (Seoul)"},{"key":"10.1016\/j.vetpar.2020.109340_bib0140","doi-asserted-by":"crossref","first-page":"3619","DOI":"10.1016\/j.vaccine.2007.01.057","article-title":"Enteral vaccination of rats against Fasciola hepatica using recombinant cysteine proteinase (cathepsin L1)","volume":"25","author":"Kesik","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2020.109340_bib0145","series-title":"Mechanisms of HLA and Disease Associations. HLA and Disease","author":"Lechler","year":"1994"},{"key":"10.1016\/j.vetpar.2020.109340_bib0150","doi-asserted-by":"crossref","first-page":"4480","DOI":"10.3382\/ps\/pez298","article-title":"Eimeria tenella: specific EtAMA1-binding peptides inhibit sporozoite entry into host cells","volume":"98","author":"Ma","year":"2019","journal-title":"Poult. Sci."},{"key":"10.1016\/j.vetpar.2020.109340_bib0155","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.rvsc.2014.07.015","article-title":"Immunological features of LPS from Ochrobactrum intermedium on sheep experimentally infected with Fasciola hepatica","volume":"97","author":"Martinez-Perez","year":"2014","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.vetpar.2020.109340_bib0160","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s00436-006-0438-y","article-title":"Prevalence of Fasciola hepatica (ELISA and fecal analysis) in ruminants from a semi-desert area in the northwest of Mexico","volume":"101","author":"Mungu\u00eda-X\u00f3chihua","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2020.109340_bib0165","doi-asserted-by":"crossref","first-page":"1949","DOI":"10.1016\/j.vaccine.2018.02.115","article-title":"Vaccination of sheep with Quil-A(R) adjuvant expands the antibody repertoire to the Fasciola MF6p\/FhHDM-1 antigen and administered together impair the growth and antigen release of flukes","volume":"36","author":"Orbegozo-Medina","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2020.109340_bib0170","doi-asserted-by":"crossref","first-page":"3234","DOI":"10.1016\/j.vaccine.2019.04.067","article-title":"Moderate protection is induced by a chimeric protein composed of leucine aminopeptidase and cathepsin L1 against Fasciola hepatica challenge in sheep","volume":"37","author":"Ortega-Vargas","year":"2019","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2020.109340_bib0175","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.tvjl.2009.07.004","article-title":"Pathological and parasitological protection in goats immunised with recombinant cathepsin L1 and challenged with Fasciola hepatica","volume":"185","author":"Perez-Ecija","year":"2010","journal-title":"Vet. J."},{"key":"10.1016\/j.vetpar.2020.109340_bib0180","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1128\/IAI.67.4.1954-1961.1999","article-title":"Vaccination with cathepsin L proteinases and with leucine aminopeptidase induces high levels of protection against fascioliasis in sheep","volume":"67","author":"Piacenza","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetpar.2020.109340_bib0185","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.vetpar.2010.04.012","article-title":"Recombinant peptides as new immunogens for the control of the bovine tick, Rhipicephalus (Boophilus) microplus","volume":"172","author":"Prudencio","year":"2010","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0190","doi-asserted-by":"crossref","DOI":"10.1016\/j.pt.2018.08.004","article-title":"Impact of human activities on fasciolosis transmission","author":"Sabourin","year":"2018","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0195","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1007\/s13238-019-0639-7","article-title":"Phage display screening of therapeutic peptide for cancer targeting and therapy","volume":"10","author":"Saw","year":"2019","journal-title":"Protein Cell"},{"key":"10.1016\/j.vetpar.2020.109340_bib0200","doi-asserted-by":"crossref","first-page":"3905","DOI":"10.4049\/jimmunol.145.11.3905","article-title":"Glutathione S-transferase. Novel vaccine against Fasciola hepatica infection in sheep","volume":"145","author":"Sexton","year":"1990","journal-title":"J. Immunol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0205","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1016\/j.vaccine.2009.01.091","article-title":"Advances in saponin-based adjuvants","volume":"27","author":"Sun","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2020.109340_bib0210","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.tvjl.2012.03.003","article-title":"Protection and antibody isotype responses against Fasciola hepatica with specific antibody to pIII-displayed peptide mimotopes of cathepsin L1 in sheep","volume":"194","author":"Villa-Mancera","year":"2012","journal-title":"Vet. J."},{"key":"10.1016\/j.vetpar.2020.109340_bib0215","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1017\/S0022149X18000792","article-title":"Bulk tank milk ELISA to detect IgG1 prevalence and clustering to determine spatial distribution and risk factors of Fasciola hepatica-infected herds in Mexico","volume":"93","author":"Villa-Mancera","year":"2019","journal-title":"J. Helminthol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0220","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.actatropica.2019.03.005","article-title":"High prevalence, potential economic impact, and risk factors of Fasciola hepatica in dairy herds in tropical, dry and temperate climate regions in Mexico","volume":"193","author":"Villa-Mancera","year":"2019","journal-title":"Acta Trop."},{"key":"10.1016\/j.vetpar.2020.109340_bib0225","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1017\/S003118200800471X","article-title":"Induction of immunity in sheep to Fasciola hepatica with mimotopes of cathepsin L selected from a phage display library","volume":"135","author":"Villa-Mancera","year":"2008","journal-title":"Parasitology"},{"key":"10.1016\/j.vetpar.2020.109340_bib0230","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1017\/S0022149X1000026X","article-title":"Proteolytic activity in Fasciola hepatica is reduced by the administration of cathepsin L mimotopes","volume":"85","author":"Villa-Mancera","year":"2011","journal-title":"J. Helminthol."},{"key":"10.1016\/j.vetpar.2020.109340_bib0235","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s00436-013-3650-6","article-title":"Cathepsin L1 mimotopes with adjuvant Quil A induces a Th1\/Th2 immune response and confers significant protection against Fasciola hepatica infection in goats","volume":"113","author":"Villa-Mancera","year":"2014","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2020.109340_bib0240","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.vetpar.2017.12.007","article-title":"Vaccination against Fasciola hepatica using cathepsin L3 and B3 proteases delivered alone or in combination","volume":"250","author":"Wesolowska","year":"2018","journal-title":"Vet. Parasitol."}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401720303204?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401720303204?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T01:34:30Z","timestamp":1759628070000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401720303204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":48,"alternative-id":["S0304401720303204"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2020.109340","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Phage display-based vaccine with cathepsin L and excretory-secretory products mimotopes of Fasciola hepatica induces protective cellular and humoral immune responses in sheep","name":"articletitle","label":"Article Title"},{"value":"Veterinary Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetpar.2020.109340","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109340"}}