{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T06:45:15Z","timestamp":1782801915967,"version":"3.54.5"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,6,10]],"date-time":"2023-06-10T00:00:00Z","timestamp":1686355200000},"content-version":"am","delay-in-days":282,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2022,9]]},"DOI":"10.1016\/j.vetpar.2022.109744","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T16:10:57Z","timestamp":1654877457000},"page":"109744","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Performance of indirect enzyme-linked immunosorbent assay using Trichinella spiralis-derived Serpin as antigen for the detection of exposure to Trichinella spp. in swine"],"prefix":"10.1016","volume":"309","author":[{"given":"Vladislav A.","family":"Lobanov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly A.","family":"Konecsni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randy W.","family":"Purves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Brad","family":"Scandrett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2022.109744_bib1","doi-asserted-by":"crossref","first-page":"5301","DOI":"10.1007\/s00253-014-5732-5","article-title":"Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production","volume":"98","author":"Ahmad","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.vetpar.2022.109744_bib2","first-page":"271","article-title":"National serologic survey for trichinellosis in sows in Canada 1996-1997","volume":"43","author":"Appleyard","year":"2002","journal-title":"Can. Vet. J."},{"key":"10.1016\/j.vetpar.2022.109744_bib3","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1093\/glycob\/cwh104","article-title":"Engineering of an artificial glycosylation pathway blocked in core oligosaccharide assembly in the yeast Pichia pastoris: production of complex humanized glycoproteins with terminal galactose","volume":"14","author":"Bobrowicz","year":"2004","journal-title":"Glycobiology"},{"key":"10.1016\/j.vetpar.2022.109744_bib4","article-title":"International commission on trichinellosis: recommendations on the use of serological tests for the detection of Trichinella infection in animals and humans","volume":"12","author":"Bruschi","year":"2019","journal-title":"Food Waterborne Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib5","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1128\/jb.173.2.627-635.1991","article-title":"Glycosylation and structure of the yeast MF alpha 1 alpha-factor precursor is important for efficient transport through the secretory pathway","volume":"173","author":"Caplan","year":"1991","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.vetpar.2022.109744_bib6","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/j.1574-6976.2000.tb00532.x","article-title":"Heterologous protein expression in the methylotrophic yeast Pichia pastoris","volume":"24","author":"Cereghino","year":"2000","journal-title":"FEMS Microbiol Rev."},{"key":"10.1016\/j.vetpar.2022.109744_bib7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-59745-456-8_1","article-title":"Introduction: distinctions between Pichia pastoris and other expression systems","volume":"389","author":"Cregg","year":"2007","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.vetpar.2022.109744_bib8","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/0014-4894(91)90086-C","article-title":"Trichinella spiralis: influence of an immunodominant, carbohydrate-associated determinant on the host antibody response repertoire","volume":"72","author":"Denkers","year":"1991","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib9","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1645\/GE-187R","article-title":"Comparison of synthetic tyvelose antigen with excretory-secretory antigen for the detection of trichinellosis in swine using enzyme-linked immunosorbent assay","volume":"90","author":"Forbes","year":"2004","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib10","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.4315\/0362-028X-62.11.1308","article-title":"A validated Trichinella digestion assay and an associated sampling and quality assurance system for use in testing pork and horse meat","volume":"62","author":"Forbes","year":"1999","journal-title":"J. Food Prot."},{"key":"10.1016\/j.vetpar.2022.109744_bib11","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1007\/s00436-008-1321-9","article-title":"Validation of a Western Blot for the detection of anti-Trichinella spp. antibodies in domestic pigs","volume":"104","author":"Frey","year":"2009","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2022.109744_bib12","first-page":"256","article-title":"Status of Trichinella spiralis in domestic swine and wild boar in Canada","volume":"61","author":"Gajadhar","year":"1997","journal-title":"Can. J. Vet. Res"},{"key":"10.1016\/j.vetpar.2022.109744_bib13","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0304-4017(01)00535-0","article-title":"An internationally recognized quality assurance system for diagnostic parasitology in animal health and food safety, with example data on trichinellosis","volume":"103","author":"Gajadhar","year":"2002","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib14","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.vetpar.2008.10.063","article-title":"Trichinella diagnostics and control: mandatory and best practices for ensuring food safety","volume":"159","author":"Gajadhar","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib15","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/0304-4017(83)90051-1","article-title":"Diagnosis of swine trichinosis by enzyme-linked immunosorbent assay (ELISA) using an excretory-secretory antigen","volume":"13","author":"Gamble","year":"1983","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib16","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/S0304-4017(00)00354-X","article-title":"International commission on trichinellosis: recommendations on methods for the control of Trichinella in domestic and wild animals intended for human consumption","volume":"93","author":"Gamble","year":"2000","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib17","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/0165-2427(84)90062-X","article-title":"Comparison of monoclonal antibody-based competitive and indirect enzyme-linked immunosorbent assays for the diagnosis of swine trichinosis","volume":"6","author":"Gamble","year":"1984","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.vetpar.2022.109744_bib18","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1051\/parasite\/20041113","article-title":"International commission on trichinellosis: recommendations on the use of serological tests for the detection of Trichinella infection in animals and man","volume":"11","author":"Gamble","year":"2004","journal-title":"Parasite"},{"key":"10.1016\/j.vetpar.2022.109744_bib19","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1128\/CMR.00026-08","article-title":"Epidemiology, diagnosis, treatment, and control of trichinellosis","volume":"22","author":"Gottstein","year":"2009","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.vetpar.2022.109744_bib20","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s13071-020-3981-y","article-title":"Molecular characterization of a Trichinella spiralis elastase-1 and its potential as a diagnostic antigen for trichinellosis","volume":"13","author":"Hu","year":"2020","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.vetpar.2022.109744_bib21","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1042\/BA20000069","article-title":"Characterization of Toxoplasma gondii surface antigen 1 (SAG1) secreted from Pichia pastoris: evidence of hyper O-glycosylation","volume":"33","author":"Letourneur","year":"2001","journal-title":"Biotechnol. Appl. Biochem."},{"key":"10.1016\/j.vetpar.2022.109744_bib22","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.aninu.2018.01.007","article-title":"Non-antibiotic feed additives in diets for pigs: a review","volume":"4","author":"Liu","year":"2018","journal-title":"Anim. Nutr."},{"key":"10.1016\/j.vetpar.2022.109744_bib23","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.3201\/eid1712.110896","article-title":"Worldwide occurrence and impact of human trichinellosis, 1986-2009","volume":"17","author":"Murrell","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.vetpar.2022.109744_bib24","first-page":"321","article-title":"Immunodiagnosis of swine trichinellosis","volume":"22","author":"Murrell","year":"1991","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"10.1016\/j.vetpar.2022.109744_bib25","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1645\/0022-3395(2003)089[0092:MCACOA]2.0.CO;2","article-title":"Molecular cloning and characterization of a serine proteinase gene of Trichinella spiralis","volume":"89","author":"Nagano","year":"2003","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib26","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1017\/S0031182001008010","article-title":"Molecular cloning and characterization of a serine proteinase inhibitor from Trichinella spiralis","volume":"123","author":"Nagano","year":"2001","journal-title":"Parasitology"},{"key":"10.1016\/j.vetpar.2022.109744_bib27","doi-asserted-by":"crossref","first-page":"93","DOI":"10.5864\/d2014-029","article-title":"Investigation of a human case of trichinellosis on a farm in southwest Ontario","volume":"57","author":"Newman","year":"2014","journal-title":"Environ. Health Rev."},{"key":"10.1016\/j.vetpar.2022.109744_bib28","first-page":"1094","article-title":"Evaluation of recombinant serine protease inhibitor from Trichinella spiralis for immunodiagnosis of swine trichinosis","volume":"43","author":"Nuamtanong","year":"2012","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"10.1016\/j.vetpar.2022.109744_bib29","unstructured":"OIE , 2019. Manual of diagnostic tests and vaccines for terrestrial animals 2019. Chapter 3.1.20. Trichinellosis (Infection with Trichinella spp.) (Paris, France, World Organisation for Animal Health (OIE))."},{"key":"10.1016\/j.vetpar.2022.109744_bib30","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetpar.2007.07.002","article-title":"World distribution of Trichinella spp. infections in animals and humans","volume":"149","author":"Pozio","year":"2007","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib31","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/j.ijpara.2013.05.010","article-title":"New pieces of the Trichinella puzzle","volume":"43","author":"Pozio","year":"2013","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib32","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1093\/glycob\/4.5.593","article-title":"Novel tyvelose-containing tri- and tetra-antennary N-glycans in the immunodominant antigens of the intracellular parasite Trichinella spiralis","volume":"4","author":"Reason","year":"1994","journal-title":"Glycobiology"},{"key":"10.1016\/j.vetpar.2022.109744_bib33","doi-asserted-by":"crossref","first-page":"4525","DOI":"10.1002\/pmic.200402057","article-title":"Proteomic analysis of the excretory-secretory proteins of the Trichinella spiralis L1 larva, a nematode parasite of skeletal muscle","volume":"5","author":"Robinson","year":"2005","journal-title":"Proteomics"},{"key":"10.1016\/j.vetpar.2022.109744_bib34","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1017\/S0950268809991518","article-title":"Comparing the demonstration of freedom from Trichinella infection of domestic pigs by traditional and risk-based surveillance","volume":"138","author":"Schuppers","year":"2010","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.vetpar.2022.109744_bib35","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.ijpara.2020.01.003","article-title":"Hiding in plain sight: discovery and phylogeography of a cryptic species of Trichinella (Nematoda: Trichinellidae) in wolverine (Gulo gulo)","volume":"50","author":"Sharma","year":"2020","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib36","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jim.2005.07.019","article-title":"De-glycosylation of Pichia pastoris-produced Schistosoma mansoni cathepsin B eliminates non-specific reactivity with IgG in normal human serum","volume":"304","author":"Stack","year":"2005","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.vetpar.2022.109744_bib37","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006485","article-title":"Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool","volume":"12","author":"Sun","year":"2018","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.vetpar.2022.109744_bib38","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1186\/s13071-015-1094-9","article-title":"Early serodiagnosis of trichinellosis by ELISA using excretory-secretory antigens of Trichinella spiralis adult worms","volume":"8","author":"Sun","year":"2015","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.vetpar.2022.109744_bib39","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1186\/s13071-015-0689-5","article-title":"Characterisation of a high-frequency gene encoding a strongly antigenic cystatin-like protein from Trichinella spiralis at its early invasion stage","volume":"8","author":"Tang","year":"2015","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.vetpar.2022.109744_bib40","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004021","article-title":"Species-specific serological detection for schistosomiasis by serine protease inhibitor (Serpin) in multiplex assay","volume":"9","author":"Tanigawa","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.vetpar.2022.109744_bib41","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0166-6851(93)90155-Q","article-title":"Characterization of novel fucosyl- and tyvelosyl-containing glycoconjugates from Trichinella spiralis muscle stage larvae","volume":"61","author":"Wisnewski","year":"1993","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2022.109744_bib42","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/1756-3305-1-27","article-title":"Trichinella spiralis: nurse cell formation with emphasis on analogy to muscle cell repair","volume":"1","author":"Wu","year":"2008","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.vetpar.2022.109744_bib43","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1016\/j.vetpar.2014.09.004","article-title":"Primary characterization and assessment of a T. spiralis antigen for the detection of Trichinella infection in pigs","volume":"205","author":"Zocevic","year":"2014","journal-title":"Vet. Parasitol."}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S030440172200098X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S030440172200098X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T01:37:21Z","timestamp":1759628241000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S030440172200098X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9]]},"references-count":43,"alternative-id":["S030440172200098X"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2022.109744","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2022,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Performance of indirect enzyme-linked immunosorbent assay using Trichinella spiralis-derived Serpin as antigen for the detection of exposure to Trichinella spp. in swine","name":"articletitle","label":"Article Title"},{"value":"Veterinary Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetpar.2022.109744","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Crown Copyright \u00a9 2022 Published by Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109744"}}