{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:10:15Z","timestamp":1792174215050,"version":"4.4.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Parasitology"],"published-print":{"date-parts":[[2023,9]]},"DOI":"10.1016\/j.vetpar.2023.110002","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T11:48:24Z","timestamp":1690890504000},"page":"110002","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Characterization of the novel glucose-methanol-choline (GMC) oxidoreductase EnOXIO1 in Eimeria necatrix"],"prefix":"10.1016","volume":"321","author":[{"given":"Feiyan","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuang","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lele","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaofeng","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjun","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vetpar.2023.110002_bib1","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/S0020-7519(02)00011-5","article-title":"Biochemical characterisation of the 56 and 82 kDa immunodominant gametocyte antigens from Eimeria maxima","volume":"32","author":"Belli","year":"2002","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2023.110002_bib2","doi-asserted-by":"crossref","first-page":"4316","DOI":"10.1016\/j.vaccine.2004.04.019","article-title":"Characterisation of the antigenic and immunogenic properties of bacterially expressed, sexual stage antigens of the coccidian parasite, Eimeria maxima","volume":"22","author":"Belli","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2023.110002_bib3","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.pt.2006.07.004","article-title":"The coccidian oocyst: a tough nut to crack!","volume":"22","author":"Belli","year":"2006","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.vetpar.2023.110002_bib4","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1128\/EC.2.3.456-464.2003","article-title":"Roles of tyrosine-rich precursor glycoproteins and dityrosine- and 3,4-dihydroxyphenylalanine-mediated protein cross-linking in development of the oocyst wall in the coccidian parasite Eimeria maxima","volume":"2","author":"Belli","year":"2003","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.vetpar.2023.110002_bib5","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0378-1119(03)00451-7","article-title":"Cloning and characterization of the 82 kDa tyrosine-rich sexual stage glycoprotein, GAM82, and its role in oocyst wall formation in the apicomplexan parasite, Eimeria maxima","volume":"307","author":"Belli","year":"2003","journal-title":"Gene"},{"key":"10.1016\/j.vetpar.2023.110002_bib6","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1007\/s00436-022-07499-3","article-title":"Characterization of the Eimeria tenella rhoptry protein with a nuclear localization sequence (EtROP30)","volume":"121","author":"Bingxiang","year":"2022","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.vetpar.2023.110002_bib7","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s13567-020-00837-2","article-title":"Re-calculating the cost of coccidiosis in chickens","volume":"51","author":"Blake","year":"2020","journal-title":"Vet. Res."},{"key":"10.1016\/j.vetpar.2023.110002_bib8","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3389\/fvets.2020.00013","article-title":"Influence of dietary zinc, copper, and manganese on the intestinal health of broilers under Eimeria challenge","volume":"7","author":"Bortoluzzi","year":"2020","journal-title":"Front Vet. Sci."},{"key":"10.1016\/j.vetpar.2023.110002_bib9","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1016\/0022-2836(92)90992-S","article-title":"GMC oxidoreductases. A newly defined family of homologous proteins with diverse catalytic activities","volume":"223","author":"Cavener","year":"1992","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.vetpar.2023.110002_bib10","doi-asserted-by":"crossref","first-page":"2683","DOI":"10.1016\/j.vaccine.2012.02.002","article-title":"Eimeria species parasites as novel vaccine delivery vectors: anti-Campylobacter jejuni protective immunity induced by Eimeria tenella-delivered CjaA","volume":"30","author":"Clark","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vetpar.2023.110002_bib11","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1111\/j.1550-7408.1973.tb03593.x","article-title":"Comparative development of Eimeria tenella from sporozoites to oocysts in primary kidney cell cultures from gallinaceous birds","volume":"20","author":"Doran","year":"1973","journal-title":"J. Protozool."},{"key":"10.1016\/j.vetpar.2023.110002_bib12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0029955","article-title":"Proteomic analysis of fractionated Toxoplasma oocysts reveals clues to their environmental resistance","volume":"7","author":"Fritz","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.vetpar.2023.110002_bib13","doi-asserted-by":"crossref","first-page":"29030","DOI":"10.1038\/srep29030","article-title":"Use of fluorescent nanoparticles to investigate nutrient acquisition by developing Eimeria maxima macrogametocytes","volume":"6","author":"Fr\u00f6lich","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.vetpar.2023.110002_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2021.109480","article-title":"Full-length transcriptome sequence analysis of Eimeria necatrix unsporulated oocysts and sporozoites identifies genes involved in cellular invasion","volume":"296","author":"Gao","year":"2021","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2023.110002_bib15","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0014-4894(71)90095-6","article-title":"Pathogenic effects of Eimeria necatrix in young chickens","volume":"30","author":"Hein","year":"1971","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2023.110002_bib16","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.parint.2015.06.002","article-title":"Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge","volume":"64","author":"Huang","year":"2015","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.vetpar.2023.110002_bib17","doi-asserted-by":"crossref","first-page":"23542","DOI":"10.1016\/S0021-9258(18)54316-7","article-title":"The sequence of squash NADH:nitrate reductase and its relationship to the sequences of other flavoprotein oxidoreductases. A family of flavoprotein pyridine nucleotide cytochrome reductases","volume":"266","author":"Hyde","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vetpar.2023.110002_bib18","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/1471-2148-7-75","article-title":"Expansion and evolution of insect GMC oxidoreductases","volume":"7","author":"Iida","year":"2007","journal-title":"BMC Evol. Biol."},{"key":"10.1016\/j.vetpar.2023.110002_bib19","doi-asserted-by":"crossref","first-page":"3906","DOI":"10.1038\/s41467-020-17675-7","article-title":"ATP-dependent hydroxylation of an unactivated primary carbon with water","volume":"11","author":"Jacoby","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.vetpar.2023.110002_bib20","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/0014-4894(70)90063-9","article-title":"Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens","volume":"28","author":"Johnson","year":"1970","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2023.110002_bib21","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1186\/s13071-015-0982-3","article-title":"Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation","volume":"8","author":"Jonscher","year":"2015","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.vetpar.2023.110002_bib22","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/nri1312","article-title":"NOX enzymes and the biology of reactive oxygen","volume":"4","author":"Lambeth","year":"2004","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.vetpar.2023.110002_bib23","article-title":"Dietary encapsulated essential oils improve production performance of coccidiosis-vaccine-challenged broiler chickens","volume":"10","author":"Lee","year":"2020","journal-title":"Anim. (Basel)"},{"key":"10.1016\/j.vetpar.2023.110002_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2022.109785","article-title":"Identification and characterization of Eimeria tenella EtTrx1 protein","volume":"310","author":"Li","year":"2022","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vetpar.2023.110002_bib25","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/1756-3305-7-27","article-title":"Cloning and characterization of an Eimeria necatrix gene encoding a gametocyte protein and associated with oocyst wall formation","volume":"7","author":"Liu","year":"2014","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.vetpar.2023.110002_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.molbiopara.2020.111318","article-title":"Identification and characterization of a cDNA encoding a gametocyte-specific protein of the avian coccidial parasite Eimeria necatrix","volume":"240","author":"Liu","year":"2020","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vetpar.2023.110002_bib27","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.vetpar.2023.110002_bib28","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1590\/S0074-02762009000200022","article-title":"Oocyst wall formation and composition in coccidian parasites","volume":"104","author":"Mai","year":"2009","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.vetpar.2023.110002_bib29","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1016\/j.ijpara.2011.07.001","article-title":"Peroxidase catalysed cross-linking of an intrinsically unstructured protein via dityrosine bonds in the oocyst wall of the apicomplexan parasite, Eimeria maxima","volume":"41","author":"Mai","year":"2011","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2023.110002_bib30","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1111\/j.1365-3024.1988.tb00251.x","article-title":"Eimeria tenella: immunological diversity between asexual generations","volume":"10","author":"McDonald","year":"1988","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.vetpar.2023.110002_bib31","first-page":"833","article-title":"Effects of artemisinin treatment to microneme gene transcription in second-generation merozoites and pathological change s of caecum in chickens infected by Eimeria tenella","volume":"45","author":"MO","year":"2014","journal-title":"Acta Vet. Et. Zootech. Sin."},{"key":"10.1016\/j.vetpar.2023.110002_bib32","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0014-4894(70)90062-7","article-title":"Coccidiosis: evaluation of coccidiostats by mortality, weight gains, and fecal scores","volume":"28","author":"Morehouse","year":"1970","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.vetpar.2023.110002_bib33","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1111\/j.1365-2958.2004.04257.x","article-title":"Redox and antioxidant systems of the malaria parasite Plasmodium falciparum","volume":"53","author":"M\u00fcller","year":"2004","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.vetpar.2023.110002_bib34","doi-asserted-by":"crossref","first-page":"17060","DOI":"10.1038\/s41598-022-20990-2","article-title":"Anticoccidial and immunogenic effectivity of encapsulated organic acids and anticoccidial drugs in broilers infected with Eimeria spp","volume":"12","author":"Nouri","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.vetpar.2023.110002_bib35","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1016\/j.ijpara.2010.06.009","article-title":"Molecular characterisation of a novel family of cysteine-rich proteins of Toxoplasma gondii and ultrastructural evidence of oocyst wall localisation","volume":"40","author":"Possenti","year":"2010","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vetpar.2023.110002_bib36","unstructured":"Sharman, P.A., 2013. Identification of enzymes potentially involved in the formation of the oocyst wall of coccidian parasites."},{"key":"10.1016\/j.vetpar.2023.110002_bib37","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1111\/j.1365-3024.2010.01209.x","article-title":"Chasing the golden egg: vaccination against poultry coccidiosis","volume":"32","author":"Sharman","year":"2010","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.vetpar.2023.110002_bib38","series-title":"Advances in Parasitology","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S0065-308X(05)60005-X","article-title":"The biology of avian Eimeria with an emphasis on their control by vaccination","author":"Shirley","year":"2005"},{"key":"10.1016\/j.vetpar.2023.110002_bib39","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1186\/s13068-019-1457-0","article-title":"The GMC superfamily of oxidoreductases revisited: analysis and evolution of fungal GMC oxidoreductases","volume":"12","author":"S\u00fctzl","year":"2019","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.vetpar.2023.110002_bib40","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/s12864-015-1298-6","article-title":"RNA Seq analysis of the Eimeria tenella gametocyte transcriptome reveals clues about the molecular basis for sexual reproduction and oocyst biogenesis","volume":"16","author":"Walker","year":"2015","journal-title":"BMC Genom."},{"key":"10.1016\/j.vetpar.2023.110002_bib41","doi-asserted-by":"crossref","first-page":"2036","DOI":"10.1128\/iai.60.5.2036-2039.1992","article-title":"Maternal immunization with gametocyte antigens as a means of providing protective immunity against Eimeria maxima in chickens","volume":"60","author":"Wallach","year":"1992","journal-title":"Infect. Immun."},{"key":"10.1016\/j.vetpar.2023.110002_bib42","first-page":"62","article-title":"Effect of different inoculation dosage on oocyst producton of Eimeria necatrix","volume":"41","author":"Wang","year":"2019","journal-title":"China Poult."},{"key":"10.1016\/j.vetpar.2023.110002_bib43","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1080\/03079450220148988","article-title":"Anticoccidial vaccines for broiler chickens: pathways to success","volume":"31","author":"Williams","year":"2002","journal-title":"Avian Pathol."},{"key":"10.1016\/j.vetpar.2023.110002_bib44","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1007\/s00436-013-3307-5","article-title":"Expression of Toxoplasma gondii dense granule protein7 (GRA7) in Eimeria tenella","volume":"112","author":"Yin","year":"2013","journal-title":"Parasitol. Res"},{"key":"10.1016\/j.vetpar.2023.110002_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2020.109032","article-title":"In vivo immunoprotective comparison between recombinant protein and DNA vaccine of Eimeria tenella surface antigen 4","volume":"278","author":"Zhao","year":"2020","journal-title":"Vet. Parasitol."}],"container-title":["Veterinary Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401723001334?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0304401723001334?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T01:38:53Z","timestamp":1759628333000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0304401723001334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9]]},"references-count":45,"alternative-id":["S0304401723001334"],"URL":"https:\/\/doi.org\/10.1016\/j.vetpar.2023.110002","relation":{},"ISSN":["0304-4017"],"issn-type":[{"value":"0304-4017","type":"print"}],"subject":[],"published":{"date-parts":[[2023,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of the novel glucose-methanol-choline (GMC) oxidoreductase EnOXIO1 in Eimeria necatrix","name":"articletitle","label":"Article Title"},{"value":"Veterinary Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vetpar.2023.110002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"110002"}}