{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T11:19:15Z","timestamp":1778757555074,"version":"3.51.4"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100018583","name":"Wuhan Science and Technology Project","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018583","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virology"],"published-print":{"date-parts":[[2025,2]]},"DOI":"10.1016\/j.virol.2025.110393","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T14:03:01Z","timestamp":1736949781000},"page":"110393","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Expression and purification of PNGase F protein in yeast and its anti-PRV activity"],"prefix":"10.1016","volume":"603","author":[{"given":"Haonan","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuda","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Furong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"11","key":"10.1016\/j.virol.2025.110393_bib1","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.3201\/eid1911.130177","article-title":"Pseudorabies virus variant in Bartha-K61-vaccinated pigs, China, 2012","volume":"19","author":"An","year":"2013","journal-title":"Emerg. Infect. Dis."},{"issue":"1","key":"10.1016\/j.virol.2025.110393_bib2","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.03718-21","article-title":"Inhibition of protein N-glycosylation blocks SARS-CoV-2 infection","volume":"13","author":"Casas-Sanchez","year":"2021","journal-title":"mBio"},{"key":"10.1016\/j.virol.2025.110393_bib3","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1255667","article-title":"Deficiency of N-linked glycosylation impairs immune function of B7-H6","volume":"14","author":"Chen","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.virol.2025.110393_bib4","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.vetmic.2015.11.022","article-title":"Pathogenicity and immunogenicity of a gE\/gI\/TK gene-deleted pseudorabies virus variant in susceptible animals","volume":"182","author":"Cong","year":"2016","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virol.2025.110393_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2020.108864","article-title":"Hydroquinone inhibits PRV infection in neurons in vitro and in vivo","volume":"250","author":"Fang","year":"2020","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virol.2025.110393_bib6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetmic.2016.11.019","article-title":"Vaccines against pseudorabies virus (PrV)","volume":"206","author":"Freuling","year":"2017","journal-title":"Vet. Microbiol."},{"issue":"4","key":"10.1016\/j.virol.2025.110393_bib7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1006696","article-title":"Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene","volume":"13","author":"Fujihira","year":"2017","journal-title":"PLoS Genet."},{"key":"10.1016\/j.virol.2025.110393_bib8","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.27612","article-title":"Tissue-specific regulation of BMP signaling by Drosophila N-glycanase 1","volume":"6","author":"Galeone","year":"2017","journal-title":"Elife"},{"issue":"5","key":"10.1016\/j.virol.2025.110393_bib9","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.1523\/JNEUROSCI.4962-08.2010","article-title":"The N-glycanase png-1 acts to limit axon branching during organ formation in Caenorhabditis elegans","volume":"30","author":"Habibi-Babadi","year":"2010","journal-title":"J. Neurosci. : Official J. Soc. Neurosci."},{"key":"10.1016\/j.virol.2025.110393_bib10","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/978-3-319-53168-7_6","article-title":"Herpesvirus capsid assembly and DNA packaging","volume":"223","author":"Heming","year":"2017","journal-title":"Adv. Anat. Embryol. Cell Biol."},{"issue":"2","key":"10.1016\/j.virol.2025.110393_bib11","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/sj.embor.7400066","article-title":"Yeast N-glycanase distinguishes between native and non-native glycoproteins","volume":"5","author":"Hirsch","year":"2004","journal-title":"EMBO Rep."},{"issue":"5","key":"10.1016\/j.virol.2025.110393_bib12","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1177\/1040638715593599","article-title":"Outbreak of variant pseudorabies virus in Bartha-K61-vaccinated piglets in central Shandong Province, China","volume":"27","author":"Hu","year":"2015","journal-title":"J. Vet. Diagn. Invest. : official Pub. Am. Assoc. Veterinary Lab. Diagnostic. Inc"},{"issue":"6","key":"10.1016\/j.virol.2025.110393_bib13","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.2460\/ajvr.1985.46.06.1359","article-title":"Attenuated properties of thymidine kinase-negative deletion mutant of pseudorabies virus","volume":"46","author":"Kit","year":"1985","journal-title":"Am. J. Vet. Res."},{"issue":"1","key":"10.1016\/j.virol.2025.110393_bib14","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1128\/JVI.78.1.424-440.2004","article-title":"Complete, annotated sequence of the pseudorabies virus genome","volume":"78","author":"Klupp","year":"2004","journal-title":"J. Virol."},{"issue":"4","key":"10.1016\/j.virol.2025.110393_bib15","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0007-1935(17)48419-3","article-title":"Experimental Aujeszky's disease (pseudorabies) in rats","volume":"126","author":"McFerran","year":"1970","journal-title":"Br. Vet. J."},{"issue":"1","key":"10.1016\/j.virol.2025.110393_bib16","first-page":"99","article-title":"Aujeszky's disease (pseudorabies) virus: the virus and molecular pathogenesis--state of the art, June 1999","volume":"31","author":"Mettenleiter","year":"2000","journal-title":"Vet. Res."},{"issue":"Pt 7","key":"10.1016\/j.virol.2025.110393_bib17","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1099\/0022-1317-71-7-1591","article-title":"Inactivation of the thymidine kinase gene of a gI deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine s train","volume":"71","author":"Moormann","year":"1990","journal-title":"J. Gen. Virol."},{"issue":"4","key":"10.1016\/j.virol.2025.110393_bib18","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1006\/jmbi.1994.1537","article-title":"Purification and crystallization of the endoglycosidase PNGase F, a peptide:N-glycosidase from Flavobacterium meningosepticum","volume":"241","author":"Norris","year":"1994","journal-title":"J. Mol. Biol."},{"issue":"4","key":"10.1016\/j.virol.2025.110393_bib19","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/0264-410X(94)90104-X","article-title":"Non-transmissible pseudorabies virus gp50 mutants: a new generation of safe live vaccines","volume":"12","author":"Peeters","year":"1994","journal-title":"Vaccine"},{"issue":"17","key":"10.1016\/j.virol.2025.110393_bib20","doi-asserted-by":"crossref","first-page":"10700","DOI":"10.1016\/S0021-9258(18)90568-5","article-title":"Demonstration of peptide:N-glycosidase F activity in endo-beta-N-acetylglucosaminidase F preparations","volume":"259","author":"Plummer","year":"1984","journal-title":"J. Biol. Chem."},{"issue":"6","key":"10.1016\/j.virol.2025.110393_bib21","first-page":"911","article-title":"[High level expression of PNGase F in Escherichia coli and its bioactivities","volume":"21","author":"Su","year":"2005","journal-title":"Sheng wu gong cheng xue bao = Chinese J. Biotech."},{"issue":"Suppl. 1","key":"10.1016\/j.virol.2025.110393_bib22","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s10068-016-0105-8","article-title":"Protective effect of resveratrol against pseudorabies virus-induced reproductive failure in a mouse model","volume":"25","author":"Su","year":"2016","journal-title":"Food Sci. Biotechnol."},{"key":"10.1016\/j.virol.2025.110393_bib23","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.vetmic.2015.12.008","article-title":"Control of swine pseudorabies in China: opportunities and limitations","volume":"183","author":"Sun","year":"2016","journal-title":"Vet. Microbiol."},{"issue":"3\u20134","key":"10.1016\/j.virol.2025.110393_bib24","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.vetmic.2015.09.021","article-title":"Emergence of a Pseudorabies virus variant with increased virulence to piglets","volume":"181","author":"Tong","year":"2015","journal-title":"Vet. Microbiol."},{"issue":"9","key":"10.1016\/j.virol.2025.110393_bib25","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00084-18","article-title":"Functional role of N-linked glycosylation in pseudorabies virus glycoprotein gH","volume":"92","author":"Vallbracht","year":"2018","journal-title":"J. Virol."},{"issue":"1","key":"10.1016\/j.virol.2025.110393_bib26","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens10010061","article-title":"Influence of N-glycosylation on expression and function of pseudorabies virus glycoprotein gB","volume":"10","author":"Vallbracht","year":"2021","journal-title":"Pathogens"},{"issue":"1","key":"10.1016\/j.virol.2025.110393_bib27","doi-asserted-by":"crossref","first-page":"61","DOI":"10.3390\/pathogens10010061","article-title":"Influence of N-glycosylation on expression and function of pseudorabies virus glycoprotein gB","volume":"10","author":"Vallbracht","year":"2021","journal-title":"Pathogens"},{"issue":"1","key":"10.1016\/j.virol.2025.110393_bib28","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1038\/s41467-022-29250-3","article-title":"Structures of pseudorabies virus capsids","volume":"13","author":"Wang","year":"2022","journal-title":"Nat. Commun."},{"issue":"4","key":"10.1016\/j.virol.2025.110393_bib29","first-page":"532","article-title":"[Construction and characterization of a pseudorabies virus TK-\/gG- mutant]","volume":"20","author":"Xu","year":"2004","journal-title":"Sheng wu gong cheng xue bao = Chinese J. Biotech."},{"issue":"1","key":"10.1016\/j.virol.2025.110393_bib30","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s13567-023-01171-z","article-title":"Membrane fusion, potential threats, and natural antiviral drugs of pseudorabies virus","volume":"54","author":"Ye","year":"2023","journal-title":"Vet. Res."},{"key":"10.1016\/j.virol.2025.110393_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2019.108543","article-title":"Construction of a US7\/US8\/UL23\/US3-deleted recombinant pseudorabies virus and evaluation of its pathogenicity in dogs","volume":"240","author":"Yin","year":"2020","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virol.2025.110393_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2023.109678","article-title":"Recombinant porcine Interferon-\u03b1 and Interleukin-2 fusion protein (rPoIFN\u03b1+IL-2) shows potent anti-pseudorabies virus activity in vitro and in vivo","volume":"279","author":"Zhao","year":"2023","journal-title":"Vet. Microbiol."},{"issue":"8","key":"10.1016\/j.virol.2025.110393_bib33","doi-asserted-by":"crossref","DOI":"10.3390\/v14081638","article-title":"Pseudorabies virus: from pathogenesis to prevention strategies","volume":"14","author":"Zheng","year":"2022","journal-title":"Viruses"},{"issue":"8","key":"10.1016\/j.virol.2025.110393_bib34","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.vaccine.2017.01.003","article-title":"Bartha-k61 vaccine protects growing pigs against challenge with an emerging variant pseudorabies virus","volume":"35","author":"Zhou","year":"2017","journal-title":"Vaccine"}],"container-title":["Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682225000054?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682225000054?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T12:58:32Z","timestamp":1769950712000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0042682225000054"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":34,"alternative-id":["S0042682225000054"],"URL":"https:\/\/doi.org\/10.1016\/j.virol.2025.110393","relation":{},"ISSN":["0042-6822"],"issn-type":[{"value":"0042-6822","type":"print"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Expression and purification of PNGase F protein in yeast and its anti-PRV activity","name":"articletitle","label":"Article Title"},{"value":"Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virol.2025.110393","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"110393"}}