{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T17:56:10Z","timestamp":1783792570827,"version":"3.55.0"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"am","delay-in-days":296,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virology"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.virol.2025.110630","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T23:48:40Z","timestamp":1753141720000},"page":"110630","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Oropouche virus infection induces pyroptosis in human THP-1 macrophages"],"prefix":"10.1016","volume":"611","author":[{"given":"Eduardo","family":"Jurado-Cobena","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cigdem","family":"Alkan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8318-2783","authenticated-orcid":false,"given":"Tetsuro","family":"Ikegami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.virol.2025.110630_bib1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0001315","article-title":"Iquitos virus: a novel reassortant orthobunyavirus associated with human illness in Peru","volume":"5","author":"Aguilar","year":"2011","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.virol.2025.110630_bib2","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/978-1-0716-3926-9_25","article-title":"Use of human macrophages to study bunyavirus NSs functions","volume":"2824","author":"Alkan","year":"2024","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.virol.2025.110630_bib3","doi-asserted-by":"crossref","first-page":"491","DOI":"10.3389\/fimmu.2014.00491","article-title":"Macrophage cytokines: involvement in immunity and infectious diseases","volume":"5","author":"Arango Duque","year":"2014","journal-title":"Front. Immunol."},{"key":"10.1016\/j.virol.2025.110630_bib4","doi-asserted-by":"crossref","DOI":"10.3201\/eid3012.241470","article-title":"Replication-competent oropouche virus in semen of traveler returning to Italy from Cuba, 2024","volume":"30","author":"Castilletti","year":"2024","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.virol.2025.110630_bib5","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1002\/jmv.24722","article-title":"Oropouche virus is detected in peripheral blood leukocytes from patients","volume":"89","author":"de Souza Luna","year":"2017","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.virol.2025.110630_bib6","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/imr.12621","article-title":"Overview of the IL-1 family in innate inflammation and acquired immunity","volume":"281","author":"Dinarello","year":"2018","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.virol.2025.110630_bib7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009494","article-title":"Orthobunyaviruses in the Caribbean: melao and oropouche virus infections in school children in Haiti in 2014","volume":"15","author":"Elbadry","year":"2021","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.virol.2025.110630_bib8","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.virol.2013.11.015","article-title":"Rift valley fever virus infection induces activation of the NLRP3 inflammasome","volume":"449","author":"Ermler","year":"2014","journal-title":"Virology"},{"key":"10.1016\/j.virol.2025.110630_bib9","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1038\/s41541-022-00456-2","article-title":"Baseline mapping of oropouche virology, epidemiology, therapeutics, and vaccine research and development","volume":"7","author":"Files","year":"2022","journal-title":"NPJ Vaccines"},{"key":"10.1016\/j.virol.2025.110630_bib10","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.00167-22","article-title":"Nonstructural protein NSs activates inflammasome and pyroptosis through interaction with NLRP3 in human microglial cells infected with severe fever with thrombocytopenia syndrome bandavirus","volume":"96","author":"Gao","year":"2022","journal-title":"J. Virol."},{"key":"10.1016\/j.virol.2025.110630_bib11","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.958098","article-title":"Resting time after phorbol 12-myristate 13-acetate in THP-1 derived macrophages provides a non-biased model for the study of NLRP3 inflammasome","volume":"13","author":"Giambelluca","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.virol.2025.110630_bib12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1012387","article-title":"RVFV virulence factor NSs triggers the mitochondrial MCL-1-BAK axis to activate pathogenic NLRP3 pyroptosis","volume":"20","author":"Guan","year":"2024","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.virol.2025.110630_bib13","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.4049\/jimmunol.1302839","article-title":"FADD and caspase-8 mediate priming and activation of the canonical and noncanonical Nlrp3 inflammasomes","volume":"192","author":"Gurung","year":"2014","journal-title":"J. Immunol."},{"key":"10.1016\/j.virol.2025.110630_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2023.107698","article-title":"Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores","volume":"26","author":"Guy","year":"2023","journal-title":"iScience"},{"key":"10.1016\/j.virol.2025.110630_bib15","doi-asserted-by":"crossref","first-page":"7262","DOI":"10.1128\/JVI.00135-15","article-title":"Rift valley fever virus MP-12 vaccine is fully attenuated by a combination of partial attenuations in the S, M, and L segments","volume":"89","author":"Ikegami","year":"2015","journal-title":"J. Virol."},{"key":"10.1016\/j.virol.2025.110630_bib16","doi-asserted-by":"crossref","first-page":"3328","DOI":"10.3390\/ijms20133328","article-title":"The NLRP3 inflammasome: an overview of mechanisms of activation and regulation","volume":"20","author":"Kelley","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.virol.2025.110630_bib17","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1146\/annurev-immunol-073119-095439","article-title":"Caspases in cell death, inflammation, and pyroptosis","volume":"38","author":"Kesavardhana","year":"2020","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.virol.2025.110630_bib18","first-page":"65","article-title":"Pyroptosis in antiviral immunity","volume":"442","author":"Kuriakose","year":"2023","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1016\/j.virol.2025.110630_bib19","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1038\/s41598-022-06229-0","article-title":"Activation of the NLRP3 inflammasome and elevation of interleukin-1beta secretion in infection by sever fever with thrombocytopenia syndrome virus","volume":"12","author":"Li","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.virol.2025.110630_bib20","article-title":"SFTSV infection induced Interleukin-1beta secretion through NLRP3 inflammasome activation","volume":"12","author":"Liu","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.virol.2025.110630_bib21","doi-asserted-by":"crossref","first-page":"e662","DOI":"10.1016\/S1473-3099(24)00557-7","article-title":"Oropouche fever: reports of vertical transmission and deaths in Brazil","volume":"24","author":"Martins-Filho","year":"2024","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.virol.2025.110630_bib22","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1128\/CMR.00046-08","article-title":"Pathogen recognition and inflammatory signaling in innate immune defenses","volume":"22","author":"Mogensen","year":"2009","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.virol.2025.110630_bib23","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.1038\/s41591-024-03300-3","article-title":"Human outbreaks of a novel reassortant oropouche virus in the Brazilian amazon region","volume":"30","author":"Naveca","year":"2024","journal-title":"Nat. Med."},{"key":"10.1016\/j.virol.2025.110630_bib24","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s00011-007-6115-5","article-title":"Optimized THP-1 differentiation is required for the detection of responses to weak stimuli","volume":"56","author":"Park","year":"2007","journal-title":"Inflamm. Res."},{"key":"10.1016\/j.virol.2025.110630_bib25","doi-asserted-by":"crossref","first-page":"4720","DOI":"10.1128\/JVI.00077-15","article-title":"Oropouche virus infection and pathogenesis are restricted by MAVS, IRF-3, IRF-7, and type I interferon signaling pathways in nonmyeloid cells","volume":"89","author":"Proenca-Modena","year":"2015","journal-title":"J. Virol."},{"key":"10.1016\/j.virol.2025.110630_bib26","doi-asserted-by":"crossref","first-page":"785","DOI":"10.3390\/v12070785","article-title":"Oropouche virus infects, persists and induces IFN response in human peripheral blood mononuclear cells as identified by RNA PrimeFlow and qRT-PCR assays","volume":"12","author":"Ribeiro Amorim","year":"2020","journal-title":"Viruses"},{"key":"10.1016\/j.virol.2025.110630_bib27","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.virusres.2010.08.009","article-title":"Oropouche virus experimental infection in the golden hamster (mesocrisetus auratus)","volume":"155","author":"Rodrigues","year":"2011","journal-title":"Virus Res."},{"key":"10.1016\/j.virol.2025.110630_bib28","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms14128","article-title":"Cleavage of DFNA5 by caspase-3 during apoptosis mediates progression to secondary necrotic\/pyroptotic cell death","volume":"8","author":"Rogers","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.virol.2025.110630_bib29","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1038\/cdd.2013.37","article-title":"AIM2 and NLRP3 inflammasomes activate both apoptotic and pyroptotic death pathways via ASC","volume":"20","author":"Sagulenko","year":"2013","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.virol.2025.110630_bib30","doi-asserted-by":"crossref","DOI":"10.3390\/v10040175","article-title":"Oropouche fever: a review","volume":"10","author":"Sakkas","year":"2018","journal-title":"Viruses"},{"key":"10.1016\/j.virol.2025.110630_bib31","article-title":"Re-emergence of oropouche virus between 2023 and 2024 in Brazil: an observational epidemiological study","author":"Scachetti","year":"2024","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.virol.2025.110630_bib32","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.chembiol.2017.03.009","article-title":"Pyroptosis and apoptosis pathways engage in bidirectional crosstalk in monocytes and macrophages","volume":"24","author":"Taabazuing","year":"2017","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.virol.2025.110630_bib33","doi-asserted-by":"crossref","DOI":"10.1099\/jgv.0.002027","article-title":"Oropouche virus: an emerging orthobunyavirus","volume":"105","author":"Tilston-Lunel","year":"2024","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.virol.2025.110630_bib34","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.3201\/eid3011.240405","article-title":"Co-Circulation of 2 oropouche virus lineages, amazon basin, Colombia, 2024","volume":"30","author":"Usuga","year":"2024","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.virol.2025.110630_bib35","doi-asserted-by":"crossref","first-page":"380","DOI":"10.3201\/eid2502.181189","article-title":"Oropouche virus-associated aseptic meningoencephalitis, southeastern Brazil","volume":"25","author":"Vernal","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.virol.2025.110630_bib36","doi-asserted-by":"crossref","DOI":"10.1093\/nsr\/nwab137","article-title":"H7N9 virus infection triggers lethal cytokine storm by activating gasdermin E-mediated pyroptosis of lung alveolar epithelial cells","volume":"9","author":"Wan","year":"2022","journal-title":"Natl. Sci. Rev."},{"key":"10.1016\/j.virol.2025.110630_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2024.114004","article-title":"A bacterial toxin co-opts caspase-3 to disable active gasdermin D and limit macrophage pyroptosis","volume":"43","author":"Wright","year":"2024","journal-title":"Cell Rep."},{"key":"10.1016\/j.virol.2025.110630_bib38","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.3892\/ijmm.2015.2162","article-title":"NLRP3 inflammasome is responsible for hantavirus inducing interleukin-1beta in THP-1 cells","volume":"35","author":"Ye","year":"2015","journal-title":"Int. J. Mol. Med."},{"key":"10.1016\/j.virol.2025.110630_bib39","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0011488","article-title":"Dabie bandavirus infection induces macrophagic pyroptosis and this process is attenuated by platelets","volume":"17","author":"Yu","year":"2023","journal-title":"PLoS Neglected Trop. Dis."}],"container-title":["Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682225002430?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682225002430?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:58:06Z","timestamp":1758589086000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0042682225002430"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":39,"alternative-id":["S0042682225002430"],"URL":"https:\/\/doi.org\/10.1016\/j.virol.2025.110630","relation":{},"ISSN":["0042-6822"],"issn-type":[{"value":"0042-6822","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Oropouche virus infection induces pyroptosis in human THP-1 macrophages","name":"articletitle","label":"Article Title"},{"value":"Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virol.2025.110630","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110630"}}