{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T01:25:46Z","timestamp":1788830746129,"version":"build-2803163510"},"reference-count":96,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:00:00Z","timestamp":1760572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009104","name":"Department of Health Research, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009104","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007334","name":"Ministry of Health and Family Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007334","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004371","name":"Tampereen Yliopisto","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004371","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virology"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.virol.2025.110713","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T06:35:30Z","timestamp":1760078130000},"page":"110713","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["RNAi therapeutics targeting Japanese encephalitis virus: Gene targets, delivery platforms, and translational barriers"],"prefix":"10.1016","volume":"613","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0231-7162","authenticated-orcid":false,"given":"Maneesh","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratima","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suman","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyam Kishor","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shashank Nand","family":"Tiwari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6938-7835","authenticated-orcid":false,"given":"Ashok","family":"Aspatwar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.virol.2025.110713_bib1","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2024.1505612","article-title":"Advancements in nanoparticle-based vaccine development against Japanese encephalitis virus: a systematic review","volume":"15","author":"Adugna","year":"2024","journal-title":"Front. Immunol."},{"key":"10.1016\/j.virol.2025.110713_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.actatropica.2022.106539","article-title":"Novel molecular approaches to combat vectors and vector-borne viruses: special focus on RNA interference (RNAi) mechanisms","volume":"233","author":"Agarwal","year":"2022","journal-title":"Acta Trop."},{"key":"10.1016\/j.virol.2025.110713_bib3","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s40035-017-0101-9","article-title":"RNAi mechanisms in Huntington's disease therapy: siRNA versus shRNA","volume":"6","author":"Aguiar","year":"2017","journal-title":"Transl. Neurodegener."},{"issue":"8","key":"10.1016\/j.virol.2025.110713_bib4","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.4103\/1673-5374.332129","article-title":"Small interfering RNAs based therapies for intracerebral hemorrhage: challenges and progress in drug delivery systems","volume":"17","author":"Almarghalani","year":"2022","journal-title":"Neural Regen Res"},{"issue":"2","key":"10.1016\/j.virol.2025.110713_bib5","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1093\/jac\/dkr487","article-title":"siRNA-mediated suppression of Japanese encephalitis virus replication in cultured cells and mice","volume":"67","author":"Anantpadma","year":"2011","journal-title":"J. Antimicrob. Chemother."},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib6","article-title":"Japanese Encephalitis outbreak in Assam, Northeastern India, January to August 2022","volume":"5","author":"Bandodkar","year":"2023","journal-title":"Global Biosecurity"},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib7","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s12865-017-0197-9","article-title":"Insight into SNPs and epitopes of E protein of newly emerged genotype-I isolates of JEV from Midnapur, West Bengal, India","volume":"18","author":"Banerjee","year":"2017","journal-title":"BMC Immunol."},{"key":"10.1016\/j.virol.2025.110713_bib8","article-title":"Development of nucleic acid-based vaccines against dengue and other mosquito-borne flaviviruses: the past, present, and future","volume":"15","author":"Bello","year":"2024","journal-title":"Front. Immunol."},{"key":"10.1016\/j.virol.2025.110713_bib9","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.jconrel.2024.11.010","article-title":"Exploring the impact of commonly used ionizable and pegylated lipids on mRNA-LNPs: a combined in vitro and preclinical perspective","volume":"377","author":"Binici","year":"2025","journal-title":"J. Contr. Release"},{"key":"10.1016\/j.virol.2025.110713_bib10","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.jconrel.2024.11.010","article-title":"Exploring the impact of commonly used ionizable and pegylated lipids on mRNA-LNPs: a combined in vitro and preclinical perspective","volume":"377","author":"Binici","year":"2025","journal-title":"J. Contr. Release"},{"issue":"9","key":"10.1016\/j.virol.2025.110713_bib11","doi-asserted-by":"crossref","first-page":"13993","DOI":"10.1021\/acsnano.1c05099","article-title":"Delivery of Oligonucleotide therapeutics: Chemical modifications, lipid nanoparticles, and extracellular vesicles","volume":"15","author":"Bost","year":"2021","journal-title":"ACS Nano"},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib12","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1093\/ageing\/20.1.45","article-title":"Neurological signs in Alzheimer's disease","volume":"20","author":"Burns","year":"1991","journal-title":"Age Ageing"},{"key":"10.1016\/j.virol.2025.110713_bib13","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.3389\/fmicb.2019.01435","article-title":"Dengue virus infection of blood-brain barrier cells: consequences of severe disease","volume":"10","author":"Calder\u00f3n-Pel\u00e1ez","year":"2019","journal-title":"Front. Microbiol."},{"issue":"6","key":"10.1016\/j.virol.2025.110713_bib14","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1111\/tpj.12835","article-title":"Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric miRNA precursors","volume":"82","author":"Carbonell","year":"2015","journal-title":"Plant J."},{"key":"10.1016\/j.virol.2025.110713_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.addr.2022.114485","article-title":"Lipid nanoparticles strategies to modify pharmacokinetics of central nervous system targeting drugs: crossing or circumventing the blood-brain barrier (BBB) to manage neurological disorders","volume":"189","author":"Correia","year":"2022","journal-title":"Adv. Drug Deliv. Rev."},{"issue":"2","key":"10.1016\/j.virol.2025.110713_bib16","doi-asserted-by":"crossref","first-page":"48","DOI":"10.59566\/IJBS.2017.13048","article-title":"Molecular mechanisms and biological functions of siRNA","volume":"13","author":"Dana","year":"2017","journal-title":"Int. J. Biomed. Sci."},{"key":"10.1016\/j.virol.2025.110713_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.addr.2024.115283","article-title":"RNA therapies for CNS diseases","volume":"208","author":"Di Francesco","year":"2024","journal-title":"Adv. Drug Deliv. Rev."},{"issue":"10","key":"10.1016\/j.virol.2025.110713_bib18","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms11102427","article-title":"Vector-Transmitted flaviviruses: an antiviral molecules overview","volume":"11","author":"Diani","year":"2023","journal-title":"Microorganisms"},{"key":"10.1016\/j.virol.2025.110713_bib19","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.neures.2021.05.007","article-title":"Metformin reduces neuroinflammation and improves cognitive functions after traumatic brain injury","volume":"172","author":"DiBona","year":"2021","journal-title":"Neurosci. Res."},{"issue":"21","key":"10.1016\/j.virol.2025.110713_bib20","doi-asserted-by":"crossref","DOI":"10.3390\/cancers14215244","article-title":"Molecular engines, therapeutic targets, and challenges in pediatric brain tumors: a special emphasis on hydrogen sulfide and RNA-Based nano-delivery","volume":"14","author":"Fahmy","year":"2022","journal-title":"Cancers (Basel)"},{"issue":"8","key":"10.1016\/j.virol.2025.110713_bib21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1007992","article-title":"NS2B\/NS3 mutations enhance the infectivity of genotype I Japanese encephalitis virus in amplifying hosts","volume":"15","author":"Fan","year":"2019","journal-title":"PLoS Pathog."},{"issue":"5","key":"10.1016\/j.virol.2025.110713_bib22","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens12050715","article-title":"Mice as an animal model for Japanese encephalitis virus research: mouse susceptibility, infection route, and viral pathogenesis","volume":"12","author":"Frank","year":"2023","journal-title":"Pathogens"},{"issue":"3","key":"10.1016\/j.virol.2025.110713_bib23","doi-asserted-by":"crossref","first-page":"393","DOI":"10.2174\/1381612822666161027111542","article-title":"Polymer nanoparticles as smart carriers for the enhanced release of therapeutic agents to the CNS","volume":"23","author":"Gagliardi","year":"2017","journal-title":"Curr. Pharm. Des."},{"issue":"2","key":"10.1016\/j.virol.2025.110713_bib24","doi-asserted-by":"crossref","DOI":"10.3390\/v16020202","article-title":"Current advances in Japanese encephalitis virus drug development","volume":"16","author":"Guo","year":"2024","journal-title":"Viruses"},{"key":"10.1016\/j.virol.2025.110713_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.addr.2022.114416","article-title":"The role of lipid components in lipid nanoparticles for vaccines and gene therapy","volume":"188","author":"Hald Albertsen","year":"2022","journal-title":"Adv. Drug Deliv. Rev."},{"issue":"7","key":"10.1016\/j.virol.2025.110713_bib26","doi-asserted-by":"crossref","DOI":"10.1002\/cti2.1067","article-title":"Harnessing host-virus evolution in antiviral therapy and immunotherapy","volume":"8","author":"Heaton","year":"2019","journal-title":"Clin Transl Immunology"},{"issue":"4","key":"10.1016\/j.virol.2025.110713_bib27","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1008260","article-title":"Japanese encephalitis virus and its mechanisms of neuroinvasion","volume":"16","author":"Hsieh","year":"2020","journal-title":"PLoS Pathog."},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib28","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1038\/s41392-020-0207-x","article-title":"Therapeutic siRNA: state of the art","volume":"5","author":"Hu","year":"2020","journal-title":"Signal Transduct. Targeted Ther."},{"issue":"9","key":"10.1016\/j.virol.2025.110713_bib29","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1007\/s00109-024-02465-1","article-title":"Metformin improves cognitive dysfunction through SIRT1\/NLRP3 pathway-mediated neuroinflammation in db\/db mice","volume":"102","author":"Hu","year":"2024","journal-title":"J. Mol. Med. (Berl.)"},{"issue":"2","key":"10.1016\/j.virol.2025.110713_bib30","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens13020120","article-title":"The dynamic landscape of capsid proteins and viral RNA interactions in Flavivirus genome packaging and virus assembly","volume":"13","author":"Jablunovsky","year":"2024","journal-title":"Pathogens"},{"issue":"24","key":"10.1016\/j.virol.2025.110713_bib31","doi-asserted-by":"crossref","DOI":"10.1002\/adma.202313389","article-title":"Engineered microorganisms for advancing tumor therapy","volume":"36","author":"Jia","year":"2024","journal-title":"Adv. Mater."},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib32","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1186\/s40001-025-02499-2","article-title":"Development and validation of optimized lentivirus-like particles for gene editing tool delivery with Gag-Only strategy","volume":"30","author":"Jia","year":"2025","journal-title":"Eur. J. Med. Res."},{"issue":"2","key":"10.1016\/j.virol.2025.110713_bib33","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s43440-022-00355-2","article-title":"Antiviral drug research for Japanese encephalitis: an updated review","volume":"74","author":"Joe","year":"2022","journal-title":"Pharmacol. Rep."},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib34","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1186\/s12929-023-00981-9","article-title":"Small interfering RNA (siRNA)-based therapeutic applications against viruses: principles, potential, and challenges","volume":"30","author":"Kang","year":"2023","journal-title":"J. Biomed. Sci."},{"key":"10.1016\/j.virol.2025.110713_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.gene.2019.144300","article-title":"Vector derived artificial miRNA mediated inhibition of West Nile virus replication and protein expression","volume":"729","author":"Karothia","year":"2020","journal-title":"Gene"},{"issue":"11","key":"10.1016\/j.virol.2025.110713_bib36","doi-asserted-by":"crossref","first-page":"2704","DOI":"10.1007\/s13346-023-01361-4","article-title":"Innate immune regulations and various siRNA modalities","volume":"13","author":"Kaushal","year":"2023","journal-title":"Drug Delivery and Translational Research"},{"issue":"4","key":"10.1016\/j.virol.2025.110713_bib37","doi-asserted-by":"crossref","DOI":"10.1002\/wrna.1640","article-title":"Artificial miRNAs as therapeutic tools: challenges and opportunities","volume":"12","author":"Kotowska-Zimmer","year":"2021","journal-title":"Wiley Interdiscip Rev RNA"},{"issue":"5","key":"10.1016\/j.virol.2025.110713_bib38","doi-asserted-by":"crossref","first-page":"1798","DOI":"10.4103\/jfmpc.jfmpc_1335_21","article-title":"Effectiveness of introduction of JEV vaccination into routine immunization program in a tribal district of Odisha","volume":"11","author":"Kshatri","year":"2022","journal-title":"J. Fam. Med. Prim. Care"},{"issue":"3","key":"10.1016\/j.virol.2025.110713_bib39","doi-asserted-by":"crossref","first-page":"383","DOI":"10.3390\/pharmaceutics17030383","article-title":"Advances in liposomal interleukin and liposomal interleukin gene therapy for cancer: a comprehensive review of preclinical studies","volume":"17","author":"Kubbara","year":"2025","journal-title":"Pharmaceutics"},{"issue":"3","key":"10.1016\/j.virol.2025.110713_bib40","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1089\/nat.2018.0721","article-title":"Lipid nanoparticles enabling gene therapies: from concepts to clinical utility","volume":"28","author":"Kulkarni","year":"2018","journal-title":"Nucleic Acid Therapeut."},{"issue":"4","key":"10.1016\/j.virol.2025.110713_bib41","doi-asserted-by":"crossref","first-page":"e96","DOI":"10.1371\/journal.pmed.0030096","article-title":"A single siRNA suppresses fatal encephalitis induced by two different flaviviruses","volume":"3","author":"Kumar","year":"2006","journal-title":"PLoS Med."},{"issue":"4","key":"10.1016\/j.virol.2025.110713_bib42","first-page":"58","article-title":"Seasonal prevalence of Japanese Encephalitis (JE) in Patna District of Bihar, India","volume":"51","author":"Kumar","year":"2019","journal-title":"J. Comm. Dis."},{"issue":"2","key":"10.1016\/j.virol.2025.110713_bib43","doi-asserted-by":"crossref","first-page":"100","DOI":"10.5455\/jmas.23328","article-title":"Acute encephalitis syndrome child patient with multi-viral co-infection: a rare case report","volume":"9","author":"Kumar","year":"2019","journal-title":"J. Med. Allied Sci."},{"issue":"5","key":"10.1016\/j.virol.2025.110713_bib44","doi-asserted-by":"crossref","first-page":"521","DOI":"10.2174\/0113892010241606231003102047","article-title":"A review of miRNA regulation in Japanese encephalitis (JEV) virus infection","volume":"25","author":"Kumar","year":"2024","journal-title":"Curr. Pharm. Biotechnol."},{"key":"10.1016\/j.virol.2025.110713_bib45","doi-asserted-by":"crossref","DOI":"10.1155\/2024\/1758662","article-title":"Serious concern of Congenital Zika Syndrome (CZS) in India: a narrative review","volume":"2024","author":"Kumar","year":"2024","journal-title":"J Pregnancy"},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib46","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1186\/s12974-020-01768-7","article-title":"Dimethyl itaconate, an itaconate derivative, exhibits immunomodulatory effects on neuroinflammation in experimental autoimmune encephalomyelitis","volume":"17","author":"Kuo","year":"2020","journal-title":"J. Neuroinflammation"},{"issue":"9","key":"10.1016\/j.virol.2025.110713_bib47","article-title":"siRNA versus miRNA as therapeutics for gene silencing","volume":"4","author":"Lam","year":"2015","journal-title":"Mol. Ther. Nucleic Acids"},{"issue":"7","key":"10.1016\/j.virol.2025.110713_bib48","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0011456","article-title":"The Aedes aegypti RNA interference response against Zika virus in the context of co-infection with dengue and chikungunya viruses","volume":"17","author":"Leggewie","year":"2023","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.virol.2025.110713_bib49","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.jconrel.2015.04.001","article-title":"Generation of a safe and effective live viral vaccine by virus self-attenuation using species-specific artificial microRNA","volume":"207","author":"Li","year":"2015","journal-title":"J. Contr. Release"},{"key":"10.1016\/j.virol.2025.110713_bib50","article-title":"The 5' and 3' untranslated regions of the Japanese Encephalitis Virus (JEV): molecular genetics and higher order structures","volume":"12","author":"Liu","year":"2021","journal-title":"Front. Microbiol."},{"issue":"3","key":"10.1016\/j.virol.2025.110713_bib51","doi-asserted-by":"crossref","DOI":"10.1007\/s12038-022-00288-1","article-title":"Involvement of host microRNAs in flavivirus-induced neuropathology: an update","volume":"47","author":"Majumdar","year":"2022","journal-title":"J. Biosci."},{"issue":"6","key":"10.1016\/j.virol.2025.110713_bib52","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1016\/j.cmet.2018.05.015","article-title":"Viral vectors for studying brain mechanisms that control energy homeostasis","volume":"27","author":"Mancini","year":"2018","journal-title":"Cell Metab."},{"key":"10.1016\/j.virol.2025.110713_bib53","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2024.117672","article-title":"Nanometrics goes beyond the size: assessment of nanoparticle concentration and encapsulation efficiency in nanocarriers","volume":"174","author":"Marques","year":"2024","journal-title":"TrAC, Trends Anal. Chem."},{"key":"10.1016\/j.virol.2025.110713_bib54","doi-asserted-by":"crossref","first-page":"775","DOI":"10.3389\/fimmu.2018.00775","article-title":"RNAi-Based identification of gene-specific nuclear cofactor networks regulating Interleukin-1 target genes","volume":"9","author":"Meier-Soelch","year":"2018","journal-title":"Front. Immunol."},{"issue":"3","key":"10.1016\/j.virol.2025.110713_bib55","doi-asserted-by":"crossref","DOI":"10.3390\/jfb13030125","article-title":"Biopolymer nanoparticles for nose-to-brain drug delivery: a new promising approach for the treatment of neurological diseases","volume":"13","author":"Montegiove","year":"2022","journal-title":"J. Funct. Biomater."},{"issue":"10","key":"10.1016\/j.virol.2025.110713_bib56","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009385","article-title":"The current burden of Japanese encephalitis and the estimated impacts of vaccination: combining estimates of the spatial distribution and transmission intensity of a zoonotic pathogen","volume":"15","author":"Moore","year":"2021","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.virol.2025.110713_bib57","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2024.1393646","article-title":"Principle, application and challenges of development siRNA-based therapeutics against bacterial and viral infections: a comprehensive review","volume":"15","author":"Motamedi","year":"2024","journal-title":"Front. Microbiol."},{"issue":"9","key":"10.1016\/j.virol.2025.110713_bib58","doi-asserted-by":"crossref","first-page":"2939","DOI":"10.1016\/j.ymthe.2024.02.025","article-title":"Therapeutic potential of RNA-enriched extracellular vesicles: the next generation in RNA delivery via biogenic nanoparticles","volume":"32","author":"Muskan","year":"2024","journal-title":"Mol. Ther."},{"key":"10.1016\/j.virol.2025.110713_bib59","doi-asserted-by":"crossref","first-page":"402","DOI":"10.3389\/fendo.2018.00402","article-title":"Overview of MicroRNA biogenesis, mechanisms of actions, and circulation","volume":"9","author":"O'Brien","year":"2018","journal-title":"Front. Endocrinol."},{"key":"10.1016\/j.virol.2025.110713_bib60","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.brainres.2016.05.013","article-title":"Effects of metformin on inflammation and short-term memory in streptozotocin-induced diabetic mice","volume":"1644","author":"Oliveira","year":"2016","journal-title":"Brain Res."},{"key":"10.1016\/j.virol.2025.110713_bib61","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.ijid.2020.07.026","article-title":"Hospital-based surveillance for Japanese encephalitis in Bangladesh, 2007-2016: implications for introduction of immunization","volume":"99","author":"Paul","year":"2020","journal-title":"Int. J. Infect. Dis."},{"key":"10.1016\/j.virol.2025.110713_bib62","doi-asserted-by":"crossref","first-page":"5981","DOI":"10.2147\/IJN.S321329","article-title":"Recent advances and challenges in gene delivery mediated by polyester-based nanoparticles","volume":"16","author":"Piperno","year":"2021","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.virol.2025.110713_bib63","series-title":"Comprehensive Supramolecular Chemistry II","first-page":"365","article-title":"5.16 - supramolecular gene transfection agents","author":"Pitard","year":"2017"},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib64","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/biologics4010001","article-title":"Recent advances in arboviral vaccines: emerging platforms and promising innovations","volume":"4","author":"Pujhari","year":"2024","journal-title":"Biologics"},{"key":"10.1016\/j.virol.2025.110713_bib65","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1471-2334-12-23","article-title":"TNF-\u03b1 promoter polymorphism: a factor contributing to the different immunological and clinical phenotypes in Japanese encephalitis","volume":"12","author":"Pujhari","year":"2012","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.virol.2025.110713_bib66","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.51027","article-title":"Estimates of the global burden of Japanese encephalitis and the impact of vaccination from 2000-2015","volume":"9","author":"Quan","year":"2020","journal-title":"eLife"},{"issue":"14","key":"10.1016\/j.virol.2025.110713_bib67","doi-asserted-by":"crossref","DOI":"10.3390\/cells14141064","article-title":"Neuroprotective effects of metformin through the modulation of neuroinflammation and oxidative stress","volume":"14","author":"Reed","year":"2025","journal-title":"Cells"},{"issue":"10","key":"10.1016\/j.virol.2025.110713_bib68","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1038\/s41573-020-0075-7","article-title":"Advances in oligonucleotide drug delivery","volume":"19","author":"Roberts","year":"2020","journal-title":"Nat. Rev. Drug Discov."},{"issue":"2","key":"10.1016\/j.virol.2025.110713_bib69","doi-asserted-by":"crossref","DOI":"10.3390\/v14020339","article-title":"In vitro inhibition of replication of Dengue virus serotypes 1-4 by siRNAs bound to non-toxic liposomes","volume":"14","author":"Rodriguez-Salazar","year":"2022","journal-title":"Viruses"},{"issue":"2","key":"10.1016\/j.virol.2025.110713_bib70","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtn.2025.102562","article-title":"Harnessing endogenous miRNA targeting ZIKV: a cutting-edge strategy to inhibit virus infection","volume":"36","author":"Rosa","year":"2025","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"10.1016\/j.virol.2025.110713_bib71","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ijdevneu.2019.01.007","article-title":"Involvement of AMP-activated protein kinase in neuroinflammation and neurodegeneration in the adult and developing brain","volume":"77","author":"Saito","year":"2019","journal-title":"Int. J. Dev. Neurosci."},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib72","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/15476286.2024.2449277","article-title":"RNA diagnostics and therapeutics: a comprehensive review","volume":"22","author":"Saju","year":"2025","journal-title":"RNA Biol."},{"key":"10.1016\/j.virol.2025.110713_bib73","series-title":"Managing Infectious Diseases","first-page":"401","article-title":"Chapter 17 - nanotherapeutics in Japanese encephalitis virus infections management, in Applications of Nanotherapeutics and Nanotheranostics","author":"Samala","year":"2025"},{"key":"10.1016\/j.virol.2025.110713_bib74","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2021.610283","article-title":"Secondary siRNAs in plants: biosynthesis, various functions, and applications in virology","volume":"12","author":"Sanan-Mishra","year":"2021","journal-title":"Front. Plant Sci."},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib75","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1365-2796.2009.02189.x","article-title":"Lipid-based nanotherapeutics for siRNA delivery","volume":"267","author":"Schroeder","year":"2010","journal-title":"J. Intern. Med."},{"issue":"6","key":"10.1016\/j.virol.2025.110713_bib76","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/s41573-019-0017-4","article-title":"The current state and future directions of RNAi-based therapeutics","volume":"18","author":"Setten","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"issue":"6","key":"10.1016\/j.virol.2025.110713_bib77","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1089\/nat.2018.0743","article-title":"Artificial MicroRNA-Mediated inhibition of Japanese encephalitis virus replication in neuronal cells","volume":"28","author":"Sharma","year":"2018","journal-title":"Nucleic Acid Therapeut."},{"key":"10.1016\/j.virol.2025.110713_bib78","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.antiviral.2014.05.008","article-title":"Lentivirus-mediated RNA interference against Japanese encephalitis virus infection in vitro and in vivo","volume":"108","author":"Shen","year":"2014","journal-title":"Antivir. Res."},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib79","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/s13052-025-01922-y","article-title":"Comparison of two nutritional risk screening tools in hospitalized children with Japanese encephalitis: a causal inference of clinical outcomes and implications for optimized management","volume":"51","author":"Shen","year":"2025","journal-title":"Ital. J. Pediatr."},{"key":"10.1016\/j.virol.2025.110713_bib80","series-title":"RNA-based Technologies for Functional Genomics in Plants","first-page":"77","article-title":"Slicing messengers by artificial designs: artificial MicroRNA induced gene silencing in Polyploid plants for functional genomics and trait modification","author":"Singh","year":"2021"},{"issue":"4","key":"10.1016\/j.virol.2025.110713_bib81","article-title":"Japanese encephalitis virus: an update on the potential antivirals and vaccines","volume":"11","author":"Srivastava","year":"2023","journal-title":"Vaccines (Basel)"},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib82","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1152\/physrev.00050.2017","article-title":"Blood-Brain barrier: from physiology to disease and back","volume":"99","author":"Sweeney","year":"2019","journal-title":"Physiol. Rev."},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib83","article-title":"Coverage of Japanese encephalitis routine vaccination among children in central India","volume":"95","author":"Tandale","year":"2023","journal-title":"J. Med. Virol."},{"issue":"2","key":"10.1016\/j.virol.2025.110713_bib84","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s44197-023-00110-7","article-title":"Incidence of Japanese encephalitis and Acute encephalitis syndrome hospitalizations in the medium-endemic Region in central India","volume":"13","author":"Tandale","year":"2023","journal-title":"J. Epidemiol Glob Health"},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib85","doi-asserted-by":"crossref","DOI":"10.3390\/v17010074","article-title":"A comprehensive review of the development and therapeutic use of antivirals in Flavivirus infection","volume":"17","author":"Tripathi","year":"2025","journal-title":"Viruses"},{"key":"10.1016\/j.virol.2025.110713_bib86","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2022.900610","article-title":"Lipid-based nanoparticles and RNA as innovative neuro-therapeutics","volume":"13","author":"Tsakiri","year":"2022","journal-title":"Front. Pharmacol."},{"issue":"5","key":"10.1016\/j.virol.2025.110713_bib87","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1038\/nrneurol.2018.30","article-title":"Japanese encephalitis - the prospects for new treatments","volume":"14","author":"Turtle","year":"2018","journal-title":"Nat. Rev. Neurol."},{"issue":"1","key":"10.1016\/j.virol.2025.110713_bib88","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/s41541-021-00338-z","article-title":"The future of Japanese encephalitis vaccination: expert recommendations for achieving and maintaining optimal JE control","volume":"6","author":"Vannice","year":"2021","journal-title":"NPJ Vaccines"},{"issue":"4","key":"10.1016\/j.virol.2025.110713_bib89","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1080\/17460441.2023.2195164","article-title":"Challenges with the discovery of RNA-based therapeutics for flaviviruses","volume":"18","author":"Wang","year":"2023","journal-title":"Expet Opin. Drug Discov."},{"issue":"3","key":"10.1016\/j.virol.2025.110713_bib90","doi-asserted-by":"crossref","DOI":"10.3390\/v15030626","article-title":"Re-Emerged genotype IV of Japanese encephalitis virus is the youngest virus in evolution","volume":"15","author":"Xu","year":"2023","journal-title":"Viruses"},{"issue":"12","key":"10.1016\/j.virol.2025.110713_bib91","doi-asserted-by":"crossref","DOI":"10.3390\/v14122686","article-title":"Molecular mechanism and role of Japanese encephalitis virus infection in central nervous system-mediated diseases","volume":"14","author":"Yadav","year":"2022","journal-title":"Viruses"},{"issue":"4","key":"10.1016\/j.virol.2025.110713_bib92","doi-asserted-by":"crossref","DOI":"10.3390\/cells11040730","article-title":"High throughput screening targeting the Dengue NS3-NS5 interface identifies antivirals against Dengue, zika and West Nile viruses","volume":"11","author":"Yang","year":"2022","journal-title":"Cells"},{"issue":"5","key":"10.1016\/j.virol.2025.110713_bib93","doi-asserted-by":"crossref","DOI":"10.1002\/rmv.2575","article-title":"Nanoparticle-Based therapies for neurotropic viral infections: mechanisms, challenges, and future prospects","volume":"34","author":"Yang","year":"2024","journal-title":"Rev. Med. Virol."},{"issue":"2","key":"10.1016\/j.virol.2025.110713_bib94","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.gene.2016.08.055","article-title":"The NS3 and NS4A genes as the targets of RNA interference inhibit replication of Japanese encephalitis virus in vitro and in vivo","volume":"594","author":"Yuan","year":"2016","journal-title":"Gene"},{"issue":"6","key":"10.1016\/j.virol.2025.110713_bib95","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1007\/s11262-018-1602-z","article-title":"Effective inhibition of different Japanese encephalitis virus genotypes by RNA interference targeting two conserved viral gene sequences in vitro and in vivo","volume":"54","author":"Yuan","year":"2018","journal-title":"Virus Genes"},{"issue":"6","key":"10.1016\/j.virol.2025.110713_bib96","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1016\/S1097-2765(02)00541-5","article-title":"Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells","volume":"9","author":"Zeng","year":"2002","journal-title":"Mol. Cell"}],"container-title":["Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682225003277?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682225003277?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T09:49:29Z","timestamp":1779616169000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0042682225003277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":96,"alternative-id":["S0042682225003277"],"URL":"https:\/\/doi.org\/10.1016\/j.virol.2025.110713","relation":{},"ISSN":["0042-6822"],"issn-type":[{"value":"0042-6822","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"RNAi therapeutics targeting Japanese encephalitis virus: Gene targets, delivery platforms, and translational barriers","name":"articletitle","label":"Article Title"},{"value":"Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virol.2025.110713","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"110713"}}