{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T16:19:58Z","timestamp":1788193198961,"version":"build-2803163510"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virology"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.virol.2026.110979","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T06:35:11Z","timestamp":1780641311000},"page":"110979","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Vaccination with a PRRSV-1 modified live vaccine provides protection against a highly virulent PRRSV-1.1 Spanish strain challenge in piglets"],"prefix":"10.1016","volume":"623","author":[{"given":"Helena","family":"Pla","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meritxell","family":"Simon-Grif\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00edlvia","family":"Cros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"del Mar Fenech","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.virol.2026.110979_bib1","doi-asserted-by":"crossref","DOI":"10.1051\/vetres:2000007","article-title":"Diagnosis of persistent or prolonged porcine reproductive and respiratory syndrome virus infections","volume":"31","author":"Benfield","year":"2000","journal-title":"Vet. Res."},{"issue":"1","key":"10.1016\/j.virol.2026.110979_bib2","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s40813-016-0029-y","article-title":"Modified-live PRRSV subtype 1 vaccine UNISTRAIN\u00ae PRRS provides a partial clinical and virological protection upon challenge with East European subtype 3 PRRSV strain Lena","volume":"2","author":"Bonckaert","year":"2016","journal-title":"Porcine Health Management"},{"key":"10.1016\/j.virol.2026.110979_bib3","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.vetmic.2018.10.001","article-title":"Efficacy of a modified-live virus vaccine in pigs experimentally infected with a highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP-PRRSV-1)","volume":"226","author":"Canelli","year":"2018","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virol.2026.110979_bib4","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.vetmic.2017.09.002","article-title":"Phenotypic characterization of a highly pathogenic Italian porcine reproductive and respiratory syndrome virus (PRRSV) type 1 subtype 1 isolate in experimentally infected pigs","volume":"210","author":"Canelli","year":"2017","journal-title":"Vet. Microbiol."},{"issue":"1","key":"10.1016\/j.virol.2026.110979_bib5","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s40813-025-00423-y","article-title":"Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strain","volume":"11","author":"Cortey","year":"2025","journal-title":"Porcine Health Management"},{"key":"10.1016\/j.virol.2026.110979_bib6","doi-asserted-by":"crossref","DOI":"10.1155\/tbed\/4847981","article-title":"A Spanish porcine reproductive and respiratory syndrome virus 1 strain is highly virulent in pigs under experimental conditions","volume":"2025","author":"Curto","year":"2025","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.virol.2026.110979_bib7","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s13567-015-0166-3","article-title":"Different clinical, virological, serological and tissue tropism outcomes of two new and one old Belgian type 1 subtype 1 porcine reproductive and respiratory syndrome virus (PRRSV) isolates","volume":"46","author":"Frydas","year":"2015","journal-title":"Vet. Res."},{"key":"10.1016\/j.virol.2026.110979_bib8","article-title":"Productive and economic impact of two PRRS increased virulence outbreaks within a swine farm in Spain","volume":"343","author":"Garc\u00eda-Flores","year":"2024"},{"issue":"6","key":"10.1016\/j.virol.2026.110979_bib9","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1177\/030098589503200606","article-title":"Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus","volume":"32","author":"Halbur","year":"1995","journal-title":"Veterinary Pathology"},{"issue":"3","key":"10.1016\/j.virol.2026.110979_bib10","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1034\/j.1399-5618.2003.00033.x","article-title":"Statistical methods for longitudinal research on bipolar disorders","volume":"5","author":"Hennen","year":"2003","journal-title":"Bipolar Disord."},{"issue":"3","key":"10.1016\/j.virol.2026.110979_bib11","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0378-1135(02)00013-5","article-title":"Characterization of the carrier state in porcine reproductive and respiratory syndrome virus infection","volume":"86","author":"Horter","year":"2002","journal-title":"Vet. Microbiol."},{"issue":"8","key":"10.1016\/j.virol.2026.110979_bib12","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1292\/jvms.58.749","article-title":"Slow-reacting and complement-requiring neutralizing antibody in swine infected with porcine reproductive and respiratory syndrome (PRRS) virus","volume":"58","author":"Jusa","year":"1996","journal-title":"J. Vet. Med. Sci."},{"issue":"1","key":"10.1016\/j.virol.2026.110979_bib13","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1746-6148-6-30","article-title":"Pathogenesis and antigenic characterization of a new East European subtype 3 porcine reproductive and respiratory syndrome virus isolate","volume":"6","author":"Karniychuk","year":"2010","journal-title":"BMC Vet. Res."},{"issue":"6","key":"10.1016\/j.virol.2026.110979_bib14","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1128\/CVI.00001-15","article-title":"Comparison of two commercial type 1 porcine reproductive and respiratory syndrome virus (PRRSV) modified live vaccines against heterologous type 1 and type 2 PRRSV challenge in growing pigs","volume":"22","author":"Kim","year":"2015","journal-title":"Clin. Vaccine Immunol."},{"issue":"5","key":"10.1016\/j.virol.2026.110979_bib15","first-page":"1327","article-title":"Effect of cellular changes and onset of humoral immunity on the replication of porcine reproductive and respiratory syndrome virus in the lungs of pigs","volume":"81","author":"Labarque","year":"2000","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.virol.2026.110979_bib16","article-title":"Comparison of host immune responses to homologous and heterologous type II porcine reproductive and respiratory syndrome virus (PRRSV) challenge in vaccinated and unvaccinated pigs","volume":"2014","author":"Li","year":"2014","journal-title":"BioMed Res. Int."},{"issue":"1","key":"10.1016\/j.virol.2026.110979_bib17","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1186\/s12917-016-0854-x","article-title":"Pathological and immunological characteristics of piglets infected experimentally with a HP-PRRSV TJ strain","volume":"12","author":"Li","year":"2016","journal-title":"BMC Vet. Res."},{"issue":"1\u20132","key":"10.1016\/j.virol.2026.110979_bib18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetimm.2015.07.003","article-title":"Innate and adaptive immunity against porcine reproductive and respiratory syndrome virus","volume":"167","author":"Loving","year":"2015","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"28","key":"10.1016\/j.virol.2026.110979_bib19","doi-asserted-by":"crossref","first-page":"3788","DOI":"10.1016\/j.vaccine.2009.03.028","article-title":"Efficacy of a modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in pigs naturally exposed to a heterologous European (Italian cluster) field strain: clinical protection and cell-mediated immunity","volume":"27","author":"Martelli","year":"2009","journal-title":"Vaccine"},{"issue":"7","key":"10.1016\/j.virol.2026.110979_bib20","doi-asserted-by":"crossref","DOI":"10.1128\/mra.00304-22","article-title":"Description of a new clade within subtype 1 of betaarterivirus suid 1 causing severe outbreaks in Spain","volume":"11","author":"Mart\u00edn-Valls","year":"2022","journal-title":"Microbiol. Resour. Announc."},{"issue":"1","key":"10.1016\/j.virol.2026.110979_bib21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40813-022-00298-3","article-title":"Introduction of a PRRSV-1 strain of increased virulence in a pig production structure in Spain: virus evolution and impact on production","volume":"9","author":"Mart\u00edn-Valls","year":"2023","journal-title":"Porcine Health Management"},{"issue":"1","key":"10.1016\/j.virol.2026.110979_bib22","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.rvsc.2007.10.003","article-title":"Simultaneous detection and genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) by real-time RT-PCR and amplicon melting curve analysis using SYBR Green","volume":"85","author":"Mart\u00ednez","year":"2008","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.virol.2026.110979_bib23","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.691145","article-title":"The ability of porcine reproductive and respiratory syndrome virus isolates to induce broadly reactive neutralizing antibodies correlates with in vivo protection","volume":"12","author":"Mart\u00ednez-Lobo","year":"2021","journal-title":"Front. Immunol."},{"issue":"3","key":"10.1016\/j.virol.2026.110979_bib24","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.tvjl.2007.05.022","article-title":"The challenge of PRRS immunology","volume":"177","author":"Mateu","year":"2008","journal-title":"Vet. J."},{"issue":"3","key":"10.1016\/j.virol.2026.110979_bib25","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/tbed.12272","article-title":"Pathology and virus distribution in the lung and lymphoid tissues of pigs experimentally inoculated with three distinct type 1 PRRS virus isolates of varying pathogenicity","volume":"63","author":"Morgan","year":"2016","journal-title":"Transbound. Emerg. Dis."},{"issue":"1\u20132","key":"10.1016\/j.virol.2026.110979_bib26","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.vetmic.2012.11.024","article-title":"Increased pathogenicity of European porcine reproductive and respiratory syndrome virus is associated with enhanced adaptive responses and viral clearance","volume":"163","author":"Morgan","year":"2013","journal-title":"Vet. Microbiol."},{"issue":"1\u20132","key":"10.1016\/j.virol.2026.110979_bib27","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.virusres.2010.08.015","article-title":"The ever-expanding diversity of porcine reproductive and respiratory syndrome virus","volume":"154","author":"Murtaugh","year":"2010","journal-title":"Virus Res."},{"issue":"Suppl. 1","key":"10.1016\/j.virol.2026.110979_bib28","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1111\/j.1865-1682.2011.01292.x","article-title":"Micro-dissecting the pathogenesis and immune response of PRRSV infection paves the way for more efficient PRRSV vaccines","volume":"59","author":"Nauwynck","year":"2012","journal-title":"Transbound. Emerg. Dis."},{"issue":"1","key":"10.1016\/j.virol.2026.110979_bib29","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1186\/s13567-016-0391-4","article-title":"Review on the transmission porcine reproductive and respiratory syndrome virus between pigs and farms and impact on vaccination","volume":"47","author":"Pileri","year":"2016","journal-title":"Vet. Res."},{"key":"10.1016\/j.virol.2026.110979_bib30","series-title":"Posit Software","article-title":"RStudio: integrated development environment for R","year":"2025"},{"issue":"3","key":"10.1016\/j.virol.2026.110979_bib31","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.tvjl.2010.07.010","article-title":"Immunisation of pigs with a major envelope protein sub-unit vaccine against porcine reproductive and respiratory syndrome virus (PRRSV) results in enhanced clinical disease following experimental challenge","volume":"189","author":"Prieto","year":"2011","journal-title":"Vet. J."},{"issue":"1","key":"10.1016\/j.virol.2026.110979_bib32","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s40813-020-00183-x","article-title":"Application of an economic calculator to determine the cost of porcine reproductive and respiratory syndrome at farm-level in 21 pig herds in Germany","volume":"7","author":"Renken","year":"2021","journal-title":"Porcine Health Management"},{"key":"10.1016\/j.virol.2026.110979_bib33","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.vetmic.2017.04.021","article-title":"Preparation for emergence of an Eastern European porcine reproductive and respiratory syndrome virus (PRRSV) strain in Western Europe: immunization with modified live virus vaccines or a field strain confers partial protection","volume":"204","author":"Renson","year":"2017","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virol.2026.110979_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2023.109830","article-title":"Complex interplay between PRRSV-1 genetic diversity, coinfections and antimicrobial use influences performance parameters in post-weaning pigs","volume":"284","author":"Romeo","year":"2023","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virol.2026.110979_bib35","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2024.1330990","article-title":"The scene of lung pathology during PRRSV-1 infection","volume":"11","author":"Ruedas-Torres","year":"2024","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.virol.2026.110979_bib36","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s40813-016-0044-z","article-title":"Emergence of a virulent porcine reproductive and respiratory syndrome virus (PRRSV) 1 strain in Lower Austria","volume":"2","author":"Sinn","year":"2016","journal-title":"Porc. Health Manag."},{"key":"10.1016\/j.virol.2026.110979_bib38","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.vetmic.2017.05.011","article-title":"Pathogenicity of three genetically diverse strains of PRRSV Type 1 in specific pathogen free pigs","volume":"209","author":"Stadejek","year":"2017","journal-title":"Vet. Microbiol."},{"issue":"1\u20132","key":"10.1016\/j.virol.2026.110979_bib39","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.vetmic.2013.02.029","article-title":"Molecular evolution of PRRSV in Europe: current state of play","volume":"165","author":"Stadejek","year":"2013","journal-title":"Vet. Microbiol."},{"issue":"25","key":"10.1016\/j.virol.2026.110979_bib40","doi-asserted-by":"crossref","first-page":"2995","DOI":"10.1016\/j.vaccine.2014.03.077","article-title":"Efficacy of an attenuated European subtype 1 porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in pigs upon challenge with the East European subtype 3 PRRSV strain Lena","volume":"32","author":"Trus","year":"2014","journal-title":"Vaccine"},{"issue":"19","key":"10.1016\/j.virol.2026.110979_bib41","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1136\/vr.142.19.510","article-title":"Effect on viraemia of an American and a European serotype PRRSV vaccine after challenge with European wild-type strains of the virus","volume":"142","author":"Van Woensel","year":"1998","journal-title":"Vet. Rec."},{"issue":"11","key":"10.1016\/j.virol.2026.110979_bib42","doi-asserted-by":"crossref","first-page":"5555","DOI":"10.1128\/JVI.00189-11","article-title":"Immune evasion of porcine reproductive and respiratory syndrome virus through glycan shielding involves both glycoprotein 5 as well as glycoprotein 3","volume":"85","author":"Vu","year":"2011","journal-title":"J. Virol."},{"issue":"1\u20132","key":"10.1016\/j.virol.2026.110979_bib43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2012.09.013","article-title":"Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence","volume":"163","author":"Weesendorp","year":"2013","journal-title":"Vet. Microbiol."},{"issue":"1\u20132","key":"10.1016\/j.virol.2026.110979_bib44","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.vetmic.2014.09.010","article-title":"Lung pathogenicity of European genotype 3 strain porcine reproductive and respiratory syndrome virus (PRRSV) differs from that of subtype 1 strains","volume":"174","author":"Weesendorp","year":"2014","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virol.2026.110979_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2025.110413","article-title":"Refining genetic classification of global porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and investigating their geographic and temporal distributions","volume":"302","author":"Yim-im","year":"2025","journal-title":"Vet. Microbiol."},{"issue":"3","key":"10.1016\/j.virol.2026.110979_bib46","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1177\/104063879400600326","article-title":"A modified serum neutralization test for the detection of antibody to porcine reproductive and respiratory syndrome virus in swine sera","volume":"6","author":"Yoon","year":"1994","journal-title":"J. Vet. Diagn. Invest."}],"container-title":["Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682226001947?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682226001947?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T16:02:20Z","timestamp":1788192140000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0042682226001947"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":45,"alternative-id":["S0042682226001947"],"URL":"https:\/\/doi.org\/10.1016\/j.virol.2026.110979","relation":{},"ISSN":["0042-6822"],"issn-type":[{"value":"0042-6822","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Vaccination with a PRRSV-1 modified live vaccine provides protection against a highly virulent PRRSV-1.1 Spanish strain challenge in piglets","name":"articletitle","label":"Article Title"},{"value":"Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virol.2026.110979","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"110979"}}