{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T07:57:48Z","timestamp":1767772668832,"version":"build-2065373602"},"reference-count":42,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virologica Sinica"],"published-print":{"date-parts":[[2022,8]]},"DOI":"10.1016\/j.virs.2022.05.002","type":"journal-article","created":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T22:56:44Z","timestamp":1652309804000},"page":"558-568","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection"],"prefix":"10.1016","volume":"37","author":[{"given":"Yingying","family":"Song","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuyu","family":"Shou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huimin","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zixiang","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nannan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feifei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9728-8893","authenticated-orcid":false,"given":"Jing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2416-7708","authenticated-orcid":false,"given":"Youhua","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.virs.2022.05.002_bib1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.3390\/v8050129","article-title":"Re-designed recombinant hepatitis b virus vectors enable efficient delivery of versatile cargo genes to hepatocytes with improved safety","volume":"8","author":"Bai","year":"2016","journal-title":"Viruses"},{"key":"10.1016\/j.virs.2022.05.002_bib2","first-page":"5189","article-title":"A\u00a0liver-specific nuclear factor interacts with the promoter region of the large surface protein gene of human hepatitis b virus","volume":"9","author":"Chang","year":"1989","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.virs.2022.05.002_bib3","first-page":"236","article-title":"Establishment of three human liver carcinoma cell lines and some of their biological characteristics in vitro","volume":"23","author":"Chen","year":"1980","journal-title":"Sci. Sin."},{"key":"10.1016\/j.virs.2022.05.002_bib4","doi-asserted-by":"crossref","first-page":"6164","DOI":"10.1128\/JVI.02721-06","article-title":"Formation of hepatitis b virus covalently closed circular DNA: removal of genome-linked protein","volume":"81","author":"Gao","year":"2007","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib5","doi-asserted-by":"crossref","first-page":"3940","DOI":"10.1128\/jvi.67.7.3940-3950.1993","article-title":"Functional interaction of nuclear factors ef-c, hnf-4, and rxr alpha with hepatitis b virus enhancer i","volume":"67","author":"Garcia","year":"1993","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib6","doi-asserted-by":"crossref","first-page":"15655","DOI":"10.1073\/pnas.232137699","article-title":"Infection of a human hepatoma cell line by hepatitis b virus","volume":"99","author":"Gripon","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.virs.2022.05.002_bib7","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1128\/JVI.01921-09","article-title":"Production and function of the cytoplasmic deproteinized relaxed circular DNA of hepadnaviruses","volume":"84","author":"Guo","year":"2010","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib8","doi-asserted-by":"crossref","first-page":"12472","DOI":"10.1128\/JVI.01123-07","article-title":"Characterization of the intracellular deproteinized relaxed circular DNA of hepatitis b virus: an intermediate of covalently closed circular DNA formation","volume":"81","author":"Guo","year":"2007","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib9","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/0022-2836(67)90307-5","article-title":"Selective extraction of polyoma DNA from infected mouse cell cultures","volume":"26","author":"Hirt","year":"1967","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.virs.2022.05.002_bib10","article-title":"Investigation of cross-contamination and misidentification of 278 widely used tumor cell lines","volume":"12","author":"Huang","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.virs.2022.05.002_bib11","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1016\/j.bbrc.2013.12.052","article-title":"Evaluation and identification of hepatitis b virus entry inhibitors using hepg2 cells overexpressing a membrane transporter ntcp","volume":"443","author":"Iwamoto","year":"2014","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.virs.2022.05.002_bib12","doi-asserted-by":"crossref","first-page":"104992","DOI":"10.1016\/j.phrs.2020.104992","article-title":"Hbv induced hepatocellular carcinoma and related potential immunotherapy","volume":"159","author":"Jia","year":"2020","journal-title":"Pharmacol. Res."},{"key":"10.1016\/j.virs.2022.05.002_bib13","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/j.jhep.2014.05.018","article-title":"Kinetics of the bile acid transporter and hepatitis b virus receptor na+\/taurocholate cotransporting polypeptide (ntcp) in hepatocytes","volume":"61","author":"Konig","year":"2014","journal-title":"J.\u00a0Hepatol."},{"key":"10.1016\/j.virs.2022.05.002_bib14","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.jhep.2019.04.010","article-title":"Efficient long-term amplification of hepatitis b virus isolates after infection of slow proliferating hepg2-ntcp cells","volume":"71","author":"Konig","year":"2019","journal-title":"J.\u00a0Hepatol."},{"key":"10.1016\/j.virs.2022.05.002_bib15","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1128\/AAC.41.8.1715","article-title":"Inducible expression of human hepatitis b virus (hbv) in stably transfected hepatoblastoma cells: a novel system for screening potential inhibitors of hbv replication","volume":"41","author":"Ladner","year":"1997","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.virs.2022.05.002_bib16","article-title":"Hepatocyte nuclear factor 1alpha downregulates hbv gene expression and replication by activating the nf-kappab signaling pathway","volume":"12","author":"Lin","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.virs.2022.05.002_bib17","doi-asserted-by":"crossref","first-page":"5069","DOI":"10.1073\/pnas.87.13.5069","article-title":"Multiple liver-specific factors bind to the hepatitis b virus core\/pregenomic promoter: trans-activation and repression by ccaat\/enhancer binding protein","volume":"87","author":"Lopez-Cabrera","year":"1990","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.virs.2022.05.002_bib18","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1002\/hep.24758","article-title":"Humanized chimeric upa mouse model for the study of hepatitis b and d virus interactions and preclinical drug evaluation","volume":"55","author":"Lutgehetmann","year":"2012","journal-title":"Hepatology"},{"key":"10.1016\/j.virs.2022.05.002_bib19","doi-asserted-by":"crossref","first-page":"2764","DOI":"10.1128\/jvi.70.5.2764-2773.1996","article-title":"A\u00a0bulged region of the hepatitis b virus rna encapsidation signal contains the replication origin for discontinuous first-strand DNA synthesis","volume":"70","author":"Nassal","year":"1996","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib20","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/S2468-1253(18)30056-6","article-title":"Global prevalence, treatment, and prevention of hepatitis b virus infection in 2016: a modelling study","volume":"3","author":"Polaris Observatory","year":"2018","journal-title":"Lancet Gastroenterol. Hepatol."},{"key":"10.1016\/j.virs.2022.05.002_bib21","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01255-18","article-title":"Robust human and murine hepatocyte culture models of hepatitis b virus infection and replication","volume":"92","author":"Qiao","year":"2018","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib22","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1099\/vir.0.006007-0","article-title":"Prospero-related homeobox protein (prox1) inhibits hepatitis b virus replication through repressing multiple cis regulatory elements","volume":"90","author":"Qin","year":"2009","journal-title":"J.\u00a0Gen. Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib23","doi-asserted-by":"crossref","first-page":"5774","DOI":"10.1128\/jvi.65.11.5774-5781.1991","article-title":"Promoter-specific transactivation of hepatitis b virus transcription by a glutamine- and proline-rich domain of hepatocyte nuclear factor 1","volume":"65","author":"Raney","year":"1991","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib24","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1006\/viro.2001.1169","article-title":"Transcription and replication of a natural hepatitis b virus nucleocapsid promoter variant is regulated in vivo by peroxisome proliferators","volume":"289","author":"Raney","year":"2001","journal-title":"Virology"},{"key":"10.1016\/j.virs.2022.05.002_bib25","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1016\/j.jhep.2017.08.002","article-title":"Note of caution: contaminations of hepatocellular cell lines","volume":"67","author":"Rebouissou","year":"2017","journal-title":"J.\u00a0Hepatol."},{"key":"10.1016\/j.virs.2022.05.002_bib26","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1124\/pr.54.1.129","article-title":"Liver-enriched transcription factors in liver function and development. Part i: the hepatocyte nuclear factor network and liver-specific gene expression","volume":"54","author":"Schrem","year":"2002","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.virs.2022.05.002_bib27","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1124\/pr.56.2.5","article-title":"Liver-enriched transcription factors in liver function and development. Part ii: the c\/ebps and d site-binding protein in cell cycle control, carcinogenesis, circadian gene regulation, liver regeneration, apoptosis, and liver-specific gene regulation","volume":"56","author":"Schrem","year":"2004","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.virs.2022.05.002_bib28","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1128\/JVI.01902-09","article-title":"Fine mapping of pre-s sequence requirements for hepatitis b virus large envelope protein-mediated receptor interaction","volume":"84","author":"Schulze","year":"2010","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib29","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1128\/MMBR.64.1.51-68.2000","article-title":"Hepatitis b virus biology","volume":"64","author":"Seeger","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.virs.2022.05.002_bib30","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.1038\/s41467-017-02304-7","article-title":"Hepatitis b virus persistence in mice reveals il-21 and il-33 as regulators of viral clearance","volume":"8","author":"Shen","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.virs.2022.05.002_bib31","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/S2468-1253(20)30307-1","article-title":"Estimating the proportion of people with chronic hepatitis b virus infection eligible for hepatitis b antiviral treatment worldwide: a systematic review and meta-analysis","volume":"6","author":"Tan","year":"2021","journal-title":"Lancet Gastroenterol. Hepatol."},{"key":"10.1016\/j.virs.2022.05.002_bib32","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1073\/pnas.98.4.1841","article-title":"Transcriptional regulation of hepatitis b virus by nuclear hormone receptors is a critical determinant of viral tropism","volume":"98","author":"Tang","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.virs.2022.05.002_bib33","doi-asserted-by":"crossref","first-page":"8572","DOI":"10.1128\/JVI.76.17.8572-8581.2002","article-title":"Mechanisms of inhibition of nuclear hormone receptor-dependent hepatitis b virus replication by hepatocyte nuclear factor 3beta","volume":"76","author":"Tang","year":"2002","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib34","first-page":"325","article-title":"Hepatitis b virus transcription and replication","volume":"14","author":"Tang","year":"2001","journal-title":"Drug News Perspect."},{"key":"10.1016\/j.virs.2022.05.002_bib35","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1016\/S0140-6736(14)60220-8","article-title":"Hepatitis b virus infection","volume":"384","author":"Trepo","year":"2014","journal-title":"Lancet"},{"key":"10.1016\/j.virs.2022.05.002_bib36","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1080\/19420862.2015.1134409","article-title":"A\u00a0human monoclonal antibody against small envelope protein of hepatitis b virus with potent neutralization effect","volume":"8","author":"Wang","year":"2016","journal-title":"mAbs"},{"key":"10.1016\/j.virs.2022.05.002_bib37","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.00049","article-title":"Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis b and d virus","volume":"1","author":"Yan","year":"2012","journal-title":"Elife"},{"key":"10.1016\/j.virs.2022.05.002_bib38","doi-asserted-by":"crossref","first-page":"8719","DOI":"10.1128\/jvi.70.12.8719-8726.1996","article-title":"Promoters for synthesis of the pre-c and pregenomic mrnas of human hepatitis b virus are genetically distinct and differentially regulated","volume":"70","author":"Yu","year":"1996","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib39","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1128\/JVI.77.4.2489-2499.2003","article-title":"Distinct modes of regulation of transcription of hepatitis b virus by the nuclear receptors hnf4alpha and coup-tf1","volume":"77","author":"Yu","year":"2003","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.virs.2022.05.002_bib40","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1002\/hep.29133","article-title":"Prmt5 restricts hepatitis b virus replication through epigenetic repression of covalently closed circular DNA transcription and interference with pregenomic rna encapsidation","volume":"66","author":"Zhang","year":"2017","journal-title":"Hepatology"},{"key":"10.1016\/j.virs.2022.05.002_bib41","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1038\/s41426-018-0189-8","article-title":"Upregulation of hbv transcription by sodium taurocholate cotransporting polypeptide at the postentry step is inhibited by the entry inhibitor myrcludex b","volume":"7","author":"Zhao","year":"2018","journal-title":"Emerg. Microb. Infect."},{"key":"10.1016\/j.virs.2022.05.002_bib42","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/s12250-017-3983-x","article-title":"Productive hbv infection of well-differentiated, hntcp-expressing human hepatoma-derived (huh7) cells","volume":"32","author":"Zhou","year":"2017","journal-title":"Virol. Sin."}],"container-title":["Virologica Sinica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1995820X22000815?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1995820X22000815?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T19:18:15Z","timestamp":1761851895000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1995820X22000815"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["S1995820X22000815"],"URL":"https:\/\/doi.org\/10.1016\/j.virs.2022.05.002","relation":{},"ISSN":["1995-820X"],"issn-type":[{"type":"print","value":"1995-820X"}],"subject":[],"published":{"date-parts":[[2022,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection","name":"articletitle","label":"Article Title"},{"value":"Virologica Sinica","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virs.2022.05.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.","name":"copyright","label":"Copyright"}]}}