{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T19:18:14Z","timestamp":1774379894184,"version":"3.50.1"},"reference-count":23,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Virus Research"],"published-print":{"date-parts":[[2007,9]]},"DOI":"10.1016\/j.virusres.2007.03.030","type":"journal-article","created":{"date-parts":[[2007,5,12]],"date-time":"2007-05-12T11:13:13Z","timestamp":1178968393000},"page":"26-33","source":"Crossref","is-referenced-by-count":11,"title":["Immunological evaluation of Escherichia coli expressed E2 protein of Western equine encephalitis virus"],"prefix":"10.1016","volume":"128","author":[{"given":"Dipankar","family":"Das","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Les P.","family":"Nagata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mavanur R.","family":"Suresh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"Pt 6","key":"10.1016\/j.virusres.2007.03.030_bib1","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1099\/0022-1317-64-6-1405","article-title":"Neutralizing and non-neutralizing monoclonal antibodies to the E2 glycoprotein of Semliki Forest virus can protect mice from lethal encephalitis","volume":"64","author":"Boere","year":"1983","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.virusres.2007.03.030_bib2","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"issue":"2","key":"10.1016\/j.virusres.2007.03.030_bib3","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0166-3542(04)00126-3","article-title":"Evaluation of a Western Equine Encephalitis recombinant E1 protein for protective immunity and diagnostics","volume":"64","author":"Das","year":"2004","journal-title":"Antivir. Res."},{"issue":"10","key":"10.1016\/j.virusres.2007.03.030_bib4","doi-asserted-by":"crossref","first-page":"3579","DOI":"10.4049\/jimmunol.156.10.3579","article-title":"Novel vaccine approaches","volume":"156","author":"Ertl","year":"1996","journal-title":"J. Immunol."},{"issue":"21","key":"10.1016\/j.virusres.2007.03.030_bib5","doi-asserted-by":"crossref","first-page":"11371","DOI":"10.1073\/pnas.93.21.11371","article-title":"Alphavirus-based expression vectors: strategies and applications","volume":"93","author":"Frolov","year":"1996","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"2","key":"10.1016\/j.virusres.2007.03.030_bib6","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/0008-8749(86)90414-4","article-title":"Identification of the inflammatory cells present in the central nervous system of normal and mast cell-deficient mice during Sindbis virus encephalitis","volume":"97","author":"Griffin","year":"1986","journal-title":"Cell Immunol."},{"key":"10.1016\/j.virusres.2007.03.030_bib7","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.1600-065X.1997.tb01013.x","article-title":"The role of antibody in recovery from alphavirus encephalitis","volume":"159","author":"Griffin","year":"1997","journal-title":"Immunol. Rev."},{"issue":"16","key":"10.1016\/j.virusres.2007.03.030_bib8","doi-asserted-by":"crossref","first-page":"5997","DOI":"10.1073\/pnas.85.16.5997","article-title":"Western equine encephalitis virus is a recombinant virus","volume":"85","author":"Hahn","year":"1988","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"5259","key":"10.1016\/j.virusres.2007.03.030_bib9","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.virusres.2007.03.030_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/027245700315743","article-title":"Construction and characterization of a novel recombinant single-chain variable fragment antibody against Western equine encephalitis virus","volume":"19","author":"Long","year":"2000","journal-title":"Hybridoma"},{"issue":"1\u20132","key":"10.1016\/j.virusres.2007.03.030_bib11","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"issue":"17\u201318","key":"10.1016\/j.virusres.2007.03.030_bib12","doi-asserted-by":"crossref","first-page":"2280","DOI":"10.1016\/j.vaccine.2005.01.032","article-title":"Efficacy of DNA vaccination against western equine encephalitis virus infection","volume":"23","author":"Nagata","year":"2005","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.virusres.2007.03.030_bib13","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1099\/0022-1317-81-1-151","article-title":"Complete genomic RNA sequence of western equine encephalitis virus and expression of the structural genes","volume":"81","author":"Netolitzky","year":"2000","journal-title":"J. Gen. Virol."},{"issue":"5","key":"10.1016\/j.virusres.2007.03.030_bib14","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1093\/oxfordjournals.aje.a121416","article-title":"Isolation of the vaccine strain of Venezuelan equine encephalomyelitis virus from mosquitoes in Louisiana","volume":"95","author":"Pedersen","year":"1972","journal-title":"Am. J. Epidemiol."},{"key":"10.1016\/j.virusres.2007.03.030_bib15","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"10.1016\/j.virusres.2007.03.030_bib16","series-title":"Fields Virology","first-page":"825","article-title":"Togaviridae: the viruses and their replication","author":"Schlesinger","year":"1996"},{"issue":"2","key":"10.1016\/j.virusres.2007.03.030_bib17","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1006\/viro.2002.1677","article-title":"Recombinant chimeric western and eastern equine encephalitis viruses as potential vaccine candidates","volume":"302","author":"Schoepp","year":"2002","journal-title":"Virology"},{"issue":"3","key":"10.1016\/j.virusres.2007.03.030_bib18","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1128\/MMBR.58.3.491-562.1994","article-title":"The alphaviruses: gene expression, replication, and evolution","volume":"58","author":"Strauss","year":"1994","journal-title":"Microbiol. Rev."},{"issue":"9","key":"10.1016\/j.virusres.2007.03.030_bib19","doi-asserted-by":"crossref","first-page":"4654","DOI":"10.1128\/JVI.65.9.4654-4664.1991","article-title":"Identification of antigenically important domains in the glycoproteins of Sindbis virus by analysis of antibody escape variants","volume":"65","author":"Strauss","year":"1991","journal-title":"J. Virol."},{"issue":"9","key":"10.1016\/j.virusres.2007.03.030_bib20","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"2","key":"10.1016\/j.virusres.2007.03.030_bib21","doi-asserted-by":"crossref","first-page":"218","DOI":"10.4269\/ajtmh.2003.68.218","article-title":"Limited potential for mosquito transmission of genetically engineered, live-attenuated western equine encephalitis virus vaccine candidates","volume":"68","author":"Turell","year":"2003","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"5872","key":"10.1016\/j.virusres.2007.03.030_bib22","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/298347a0","article-title":"Synthesis and assembly of hepatitis B virus surface antigen particles in yeast","volume":"298","author":"Valenzuela","year":"1982","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.virusres.2007.03.030_bib23","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.pep.2004.05.016","article-title":"Characterisation of bacterially expressed structural protein E2 of hepatitis C virus","volume":"37","author":"Yurkova","year":"2004","journal-title":"Protein Exp. Purif."}],"container-title":["Virus Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170207001189?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170207001189?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T20:18:01Z","timestamp":1683836281000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168170207001189"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":23,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["S0168170207001189"],"URL":"https:\/\/doi.org\/10.1016\/j.virusres.2007.03.030","relation":{},"ISSN":["0168-1702"],"issn-type":[{"value":"0168-1702","type":"print"}],"subject":[],"published":{"date-parts":[[2007,9]]}}}