{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:43:17Z","timestamp":1790836997162,"version":"4.1.0"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"University of Quind\u00edo to JCCO"},{"name":"John Sealy Memorial Endowment Fund"},{"DOI":"10.13039\/100000124","name":"NIH ORWH","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000124","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NICHD"},{"name":"NIH\/NIAID"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virus Research"],"published-print":{"date-parts":[[2018,2]]},"DOI":"10.1016\/j.virusres.2017.12.013","type":"journal-article","created":{"date-parts":[[2017,12,30]],"date-time":"2017-12-30T02:40:13Z","timestamp":1514601613000},"page":"1-11","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"special_numbering":"C","title":["K48-linked polyubiquitination of dengue virus NS1 protein inhibits its interaction with the viral partner NS4B"],"prefix":"10.1016","volume":"246","author":[{"given":"Maria Isabel","family":"Giraldo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Vargas-Cuartas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Gallego-Gomez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Yong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Padilla-Sanabria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jhon Carlos","family":"Casta\u00f1o-Osorio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Rajsbaum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"6173","key":"10.1016\/j.virusres.2017.12.013_bib0005","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1126\/science.1247749","article-title":"Flavivirus NS1 structures reveal surfaces for associations with membranes and the immune system","volume":"343","author":"Akey","year":"2014","journal-title":"Science"},{"issue":"11","key":"10.1016\/j.virusres.2017.12.013_bib0010","doi-asserted-by":"crossref","first-page":"5408","DOI":"10.1128\/JVI.02188-08","article-title":"NS5 of dengue virus mediates STAT2 binding and degradation","volume":"83","author":"Ashour","year":"2009","journal-title":"J. Virol."},{"issue":"5","key":"10.1016\/j.virusres.2017.12.013_bib0015","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.chom.2010.10.007","article-title":"Mouse STAT2 restricts early dengue virus replication","volume":"8","author":"Ashour","year":"2010","journal-title":"Cell Host Microbe"},{"issue":"9","key":"10.1016\/j.virusres.2017.12.013_bib0020","doi-asserted-by":"crossref","first-page":"e1005880","DOI":"10.1371\/journal.ppat.1005880","article-title":"The matrix protein of nipah virus targets the E3-Ubiquitin ligase TRIM6 to inhibit the IKKepsilon kinase-mediated type-I IFN antiviral response","volume":"12","author":"Bharaj","year":"2016","journal-title":"PLoS Pathog."},{"issue":"18","key":"10.1016\/j.virusres.2017.12.013_bib0025","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00833-17","article-title":"The host E3-ubiquitin ligase TRIM6 ubiquitinates the ebola virus VP35 protein and promotes virus replication","volume":"91","author":"Bharaj","year":"2017","journal-title":"J. Virol."},{"issue":"7446","key":"10.1016\/j.virusres.2017.12.013_bib0030","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.virusres.2017.12.013_bib0035","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1146\/annurev.micro.56.012302.160654","article-title":"The molecular biology of West Nile Virus: a new invader of the western hemisphere","volume":"56","author":"Brinton","year":"2002","journal-title":"Annu. Rev. Microbiol."},{"issue":"3","key":"10.1016\/j.virusres.2017.12.013_bib0040","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00804-16","article-title":"Dengue virus genome uncoating requires ubiquitination","volume":"7","author":"Byk","year":"2016","journal-title":"MBio"},{"issue":"2","key":"10.1016\/j.virusres.2017.12.013_bib0045","doi-asserted-by":"crossref","first-page":"386","DOI":"10.3390\/cells3020386","article-title":"The ubiquitin-conjugating system: multiple roles in viral replication and infection","volume":"3","author":"Calistri","year":"2014","journal-title":"Cells"},{"issue":"4","key":"10.1016\/j.virusres.2017.12.013_bib0050","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1080\/10409238.2017.1325829","article-title":"Ubiquitin enzymes in the regulation of immune responses","volume":"52","author":"Ebner","year":"2017","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"issue":"11","key":"10.1016\/j.virusres.2017.12.013_bib0055","doi-asserted-by":"crossref","first-page":"4285","DOI":"10.1073\/pnas.1322036111","article-title":"Structural basis of Flavivirus NS1 assembly and antibody recognition","volume":"111","author":"Edeling","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"3","key":"10.1016\/j.virusres.2017.12.013_bib0060","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1007\/s12250-014-3459-1","article-title":"Critical role of dengue virus NS1 protein in viral replication","volume":"29","author":"Fan","year":"2014","journal-title":"Virol. Sin."},{"issue":"6","key":"10.1016\/j.virusres.2017.12.013_bib0065","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1128\/JVI.02483-10","article-title":"Appraising the roles of CBLL1 and the ubiquitin\/proteasome system for flavivirus entry and replication","volume":"85","author":"Fernandez-Garcia","year":"2011","journal-title":"J. Virol."},{"issue":"2","key":"10.1016\/j.virusres.2017.12.013_bib0070","doi-asserted-by":"crossref","first-page":"e86","DOI":"10.1371\/journal.pntd.0000086","article-title":"Host gene expression profiling of dengue virus infection in cell lines and patients","volume":"1","author":"Fink","year":"2007","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"6","key":"10.1016\/j.virusres.2017.12.013_bib0075","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.chom.2007.09.014","article-title":"Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses","volume":"2","author":"Frias-Staheli","year":"2007","journal-title":"Cell Host Microbe"},{"issue":"3","key":"10.1016\/j.virusres.2017.12.013_bib0080","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1128\/CMR.11.3.480","article-title":"Dengue and dengue hemorrhagic fever","volume":"11","author":"Gubler","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"issue":"19","key":"10.1016\/j.virusres.2017.12.013_bib0085","doi-asserted-by":"crossref","first-page":"8003","DOI":"10.1073\/pnas.1017338108","article-title":"Secreted dengue virus nonstructural protein NS1 is an atypical barrel-shaped high-density lipoprotein","volume":"108","author":"Gutsche","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"5","key":"10.1016\/j.virusres.2017.12.013_bib0090","doi-asserted-by":"crossref","DOI":"10.3390\/v9050097","article-title":"Historical perspectives on flavivirus research","volume":"9","author":"Holbrook","year":"2017","journal-title":"Viruses"},{"key":"10.1016\/j.virusres.2017.12.013_bib0095","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1146\/annurev-biochem-051810-094654","article-title":"Ubiquitin-binding proteins: decoders of ubiquitin-mediated cellular functions","volume":"81","author":"Husnjak","year":"2012","journal-title":"Annu. Rev. Biochem."},{"issue":"9","key":"10.1016\/j.virusres.2017.12.013_bib0100","doi-asserted-by":"crossref","first-page":"4687","DOI":"10.1128\/JVI.00118-14","article-title":"Ultrastructural characterization and three-dimensional architecture of replication sites in dengue virus-infected mosquito cells","volume":"88","author":"Junjhon","year":"2014","journal-title":"J. Virol."},{"issue":"10","key":"10.1016\/j.virusres.2017.12.013_bib0105","doi-asserted-by":"crossref","first-page":"4960","DOI":"10.1021\/pr100219y","article-title":"The ubiquitin-proteasome pathway is important for dengue virus infection in primary human endothelial cells","volume":"9","author":"Kanlaya","year":"2010","journal-title":"J. Proteome Res."},{"issue":"7","key":"10.1016\/j.virusres.2017.12.013_bib0110","doi-asserted-by":"crossref","first-page":"3253","DOI":"10.1128\/JVI.74.7.3253-3263.2000","article-title":"cis- and trans-acting elements in flavivirus RNA replication","volume":"74","author":"Khromykh","year":"2000","journal-title":"J. Virol."},{"issue":"9","key":"10.1016\/j.virusres.2017.12.013_bib0115","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02234-16","article-title":"Endoplasmic reticulum protein SCAP inhibits dengue virus NS2B3 protease by suppressing its K27-Linked polyubiquitylation","volume":"91","author":"Liu","year":"2017","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2017.12.013_bib0120","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.coviro.2015.09.005","article-title":"Interplay between the virus and the ubiquitin-proteasome system: molecular mechanism of viral pathogenesis","volume":"17","author":"Luo","year":"2016","journal-title":"Curr. Opin. Virol."},{"issue":"1","key":"10.1016\/j.virusres.2017.12.013_bib0125","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1006\/viro.1996.0307","article-title":"Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication","volume":"220","author":"Mackenzie","year":"1996","journal-title":"Virology"},{"issue":"304","key":"10.1016\/j.virusres.2017.12.013_bib0130","doi-asserted-by":"crossref","first-page":"304ra142","DOI":"10.1126\/scitranslmed.aaa3863","article-title":"Dengue virus NS1 protein activates cells via Toll-like receptor 4 and disrupts endothelial cell monolayer integrity","volume":"7","author":"Modhiran","year":"2015","journal-title":"Sci. Transl. Med."},{"issue":"3","key":"10.1016\/j.virusres.2017.12.013_bib0135","doi-asserted-by":"crossref","first-page":"e1003265","DOI":"10.1371\/journal.ppat.1003265","article-title":"Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling","volume":"9","author":"Morrison","year":"2013","journal-title":"PLoS Pathog."},{"issue":"2","key":"10.1016\/j.virusres.2017.12.013_bib0140","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.antiviral.2013.03.008","article-title":"The flavivirus NS1 protein: molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic biomarker","volume":"98","author":"Muller","year":"2013","journal-title":"Antiviral. Res."},{"issue":"13","key":"10.1016\/j.virusres.2017.12.013_bib0145","doi-asserted-by":"crossref","first-page":"8004","DOI":"10.1128\/JVI.79.13.8004-8013.2005","article-title":"Inhibition of alpha\/beta interferon signaling by the NS4B protein of flaviviruses","volume":"79","author":"Munoz-Jordan","year":"2005","journal-title":"J. Virol."},{"issue":"1","key":"10.1016\/j.virusres.2017.12.013_bib0150","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1006\/viro.1996.0406","article-title":"Mutagenesis of the N-linked glycosylation sites of the yellow fever virus NS1 protein: effects on virus replication and mouse neurovirulence","volume":"222","author":"Muylaert","year":"1996","journal-title":"Virology"},{"issue":"3","key":"10.1016\/j.virusres.2017.12.013_bib0155","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1007\/s00705-013-1849-6","article-title":"Inhibitory effects of curcumin on dengue virus type 2-infected cells in vitro","volume":"159","author":"Padilla","year":"2014","journal-title":"Arch. Virol."},{"issue":"2","key":"10.1016\/j.virusres.2017.12.013_bib0160","doi-asserted-by":"crossref","first-page":"32","DOI":"10.5501\/wjv.v2.i2.32","article-title":"Architecture and biogenesis of plus-strand RNA virus replication factories","volume":"2","author":"Paul","year":"2013","journal-title":"World J. Virol."},{"issue":"7","key":"10.1016\/j.virusres.2017.12.013_bib0165","doi-asserted-by":"crossref","first-page":"e1005738","DOI":"10.1371\/journal.ppat.1005738","article-title":"Dengue virus NS1 disrupts the endothelial glycocalyx: leading to hyperpermeability","volume":"12","author":"Puerta-Guardo","year":"2016","journal-title":"PLoS Pathog."},{"issue":"8","key":"10.1016\/j.virusres.2017.12.013_bib0170","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.tim.2013.06.006","article-title":"Viral evasion mechanisms of early antiviral responses involving regulation of ubiquitin pathways","volume":"21","author":"Rajsbaum","year":"2013","journal-title":"Trends Microbiol."},{"issue":"6208","key":"10.1016\/j.virusres.2017.12.013_bib0175","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1126\/science.1261509","article-title":"Virology. Unanchored ubiquitin in virus uncoating","volume":"346","author":"Rajsbaum","year":"2014","journal-title":"Science"},{"issue":"6","key":"10.1016\/j.virusres.2017.12.013_bib0180","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1016\/j.jmb.2013.12.005","article-title":"TRIMmunity: the roles of the TRIM E3-ubiquitin ligase family in innate antiviral immunity","volume":"426","author":"Rajsbaum","year":"2014","journal-title":"J. Mol. Biol."},{"issue":"6","key":"10.1016\/j.virusres.2017.12.013_bib0185","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1016\/j.immuni.2014.04.018","article-title":"Unanchored K48-linked polyubiquitin synthesized by the E3-ubiquitin ligase TRIM6 stimulates the interferon-IKKepsilon kinase-mediated antiviral response","volume":"40","author":"Rajsbaum","year":"2014","journal-title":"Immunity"},{"key":"10.1016\/j.virusres.2017.12.013_bib0190","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s12985-016-0590-7","article-title":"Flavivirus NS1: a multifaceted enigmatic viral protein","volume":"13","author":"Rastogi","year":"2016","journal-title":"Virol. J."},{"issue":"11","key":"10.1016\/j.virusres.2017.12.013_bib0195","doi-asserted-by":"crossref","first-page":"e1005277","DOI":"10.1371\/journal.ppat.1005277","article-title":"Dengue virus non-structural protein 1 modulates infectious particle production via interaction with the structural proteins","volume":"11","author":"Scaturro","year":"2015","journal-title":"PLoS Pathog."},{"issue":"3","key":"10.1016\/j.virusres.2017.12.013_bib0200","doi-asserted-by":"crossref","first-page":"674","DOI":"10.3390\/cells3030674","article-title":"Mechanisms of generating polyubiquitin chains of different topology","volume":"3","author":"Suryadinata","year":"2014","journal-title":"Cells"},{"key":"10.1016\/j.virusres.2017.12.013_bib0205","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1186\/1471-2105-9-310","article-title":"Computational identification of ubiquitylation sites from protein sequences","volume":"9","author":"Tung","year":"2008","journal-title":"BMC Bioinf."},{"issue":"3","key":"10.1016\/j.virusres.2017.12.013_bib0210","article-title":"The TRIMendous role of TRIMs in virus-host interactions","volume":"5","author":"van Tol","year":"2017","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.virusres.2017.12.013_bib0215","series-title":"Dengue Guidelines for Diagnosis, Treatment, Prevention and Control","author":"WHO,","year":"2009"},{"key":"10.1016\/j.virusres.2017.12.013_bib0220","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.antiviral.2016.02.014","article-title":"The many faces of the flavivirus NS1 protein offer a multitude of options for inhibitor design","volume":"130","author":"Watterson","year":"2016","journal-title":"Antiviral Res."},{"issue":"4","key":"10.1016\/j.virusres.2017.12.013_bib0225","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.chom.2009.03.007","article-title":"Composition and three-dimensional architecture of the dengue virus replication and assembly sites","volume":"5","author":"Welsch","year":"2009","journal-title":"Cell Host Microbe"},{"issue":"6","key":"10.1016\/j.virusres.2017.12.013_bib0230","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/ncb3358","article-title":"The increasing complexity of the ubiquitin code","volume":"18","author":"Yau","year":"2016","journal-title":"Nat. Cell Biol."},{"issue":"9","key":"10.1016\/j.virusres.2017.12.013_bib0235","doi-asserted-by":"crossref","first-page":"e75188","DOI":"10.1371\/journal.pone.0075188","article-title":"Heat shock protein 70 is associated with replicase complex of Japanese encephalitis virus and positively regulates viral genome replication","volume":"8","author":"Ye","year":"2013","journal-title":"PLoS One"},{"issue":"21","key":"10.1016\/j.virusres.2017.12.013_bib0240","doi-asserted-by":"crossref","first-page":"11815","DOI":"10.1128\/JVI.01022-12","article-title":"Flavivirus replication complex assembly revealed by DNAJC14 functional mapping","volume":"86","author":"Yi","year":"2012","journal-title":"J. Virol."},{"issue":"13","key":"10.1016\/j.virusres.2017.12.013_bib0245","doi-asserted-by":"crossref","first-page":"7360","DOI":"10.1128\/JVI.00157-12","article-title":"Evidence for a genetic and physical interaction between nonstructural proteins NS1 and NS4B that modulates replication of West Nile virus","volume":"86","author":"Youn","year":"2012","journal-title":"J. Virol."}],"container-title":["Virus Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170217305828?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170217305828?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T14:04:30Z","timestamp":1759154670000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168170217305828"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":49,"alternative-id":["S0168170217305828"],"URL":"https:\/\/doi.org\/10.1016\/j.virusres.2017.12.013","relation":{},"ISSN":["0168-1702"],"issn-type":[{"value":"0168-1702","type":"print"}],"subject":[],"published":{"date-parts":[[2018,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"K48-linked polyubiquitination of dengue virus NS1 protein inhibits its interaction with the viral partner NS4B","name":"articletitle","label":"Article Title"},{"value":"Virus Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virusres.2017.12.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}